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1. 发明申请

WO2012033382A3 NOVEL RNA-BINDING PROTEIN AND LABEL-FREE DETECTION METHOD FOR DETECTING MICRORNA USING SAME 审中-公开
标题翻译：新型RNA结合蛋白和用于检测MICRORNA的无标记检测方法
公开(公告)号：WO2012033382A3
公开(公告)日：2012-06-28
申请号：PCT/KR2011006710
申请日：2011-09-09
申请人： KOREA RES INST OF BIOSCIENCE , JUNG YONG WON , CHUNG BONG HYUN , LEE JEONG MIN
发明人： JUNG YONG WON , CHUNG BONG HYUN , LEE JEONG MIN
IPC分类号： C07K19/00 , C12N15/62 , C12Q1/68 , G01N33/68
CPC分类号： C12Q1/6809 , C07K14/47 , C07K14/52 , C07K2319/00 , C12N9/22 , C12Q2522/101 , C12Q2525/207
摘要： The present invention relates to a novel RNA-binding protein, to a composition comprising same for detecting microRNA, and to a label-free detection method for detecting microRNA using same. According to the present invention, the method for detecting microRNA using the RNA-binding protein, which is a fusion protein comprising a PAZ domain and a double stranded RNA-binding domain and which binds to double stranded RNA having an overhanging 3' terminal, is a label-free detection method for detecting microRNA without an enzymatic labeling process or enzymatic amplifying reaction, which may overcome the drawbacks of conventional microRNA analysis methods, that is, methods for analyzing microRNA after enzymatic labeling or methods for coupling an antibody to microRNA, wherein the drawbacks may include complicated processes, or difficulties developing an RNA-structure-specific antibody resulting from an instable RNA structure. Thus, the method of the present invention may be utilized in the study of gene expression in a variety of diseases, in the detection of diagnostic markers of diseases, and in the evaluation of drug efficacy.
摘要翻译：本发明涉及一种新型RNA结合蛋白，涉及包含其的用于检测微小RNA的组合物以及使用其检测微小RNA的无标记检测方法。根据本发明，使用作为包含PAZ结构域和双链RNA结合结构域并与具有突出端3'末端的双链RNA结合的融合蛋白的RNA结合蛋白检测微小RNA的方法是用于检测没有酶标记过程或酶促扩增反应的微RNA的无标记检测方法，其可以克服常规微RNA分析方法的缺点，即在酶标记之后分析微小RNA的方法或将抗体偶联到微小RNA上的方法，其中缺点可能包括复杂的过程，或由不稳定的RNA结构产生的RNA结构特异性抗体的困难。因此，本发明的方法可用于研究各种疾病中的基因表达，检测疾病的诊断标志物和药物功效的评价。

2. 发明申请

WO2012067402A2 RED FLUORESCENT PROTEIN FRAGMENT HAVING A SELF-ASSEMBLY ACTIVITY, METHOD FOR PREPARING SAME, AND METHOD FOR ANALYZING PROTEIN-PROTEIN INTERACTIONS USING SAME 审中-公开
公开(公告)号：WO2012067402A2
公开(公告)日：2012-05-24
申请号：PCT/KR2011008697
申请日：2011-11-15
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG BONG HYUN , CHUNG YOUNG WON , KEEM JOO OAK
发明人： CHUNG BONG HYUN , CHUNG YOUNG WON , KEEM JOO OAK
IPC分类号： C07K14/435 , C12N15/12 , G01N33/52 , G01N33/68
CPC分类号： C07K14/43595 , C07K2319/00 , G01N33/533
摘要： The present invention relates to a red fluorescent protein fragment having a self-assembly activity, to a method for preparing same, and to a method for analyzing protein-protein interactions using same. The red fluorescent protein fragment (mPlum 1 E16V) according to the present invention is prepared by coupling the ß-strand at position 1 of the red fluorescent protein (mPlum) after the ß-strands at positions 2 to 11 via a flexible peptide linker so as to prepare a circularly permutated mPlum protein (CpmPlum), replacing glutamic acid (Glu) at position 16 of the amino acid sequence of the ß-strand at position 1 of the circularly permutated mPlum protein (CpmPlum) with valine (Val) so as to prepare a mutant (CpmPlum E16V), separating the mPlum 1 peptide (mPlum 1 E16V), which is mutated alone, from mPlum 2-11 protein and self-assembling the separated mPlum 1 peptide and mPlum 2-11 protein in order to emit a red fluorescent signal, thus enabling the red fluorescent protein fragment to have a self-assembly activity. Accordingly, the red fluorescent protein fragment (mPlum 1 E16V) having a self-assembly activity according to the present invention may serve as a biosensor that can be effectively used in analyzing the position of a protein in a living cell and the function of a protein such as protein-protein interaction.

3. 发明申请

WO2011136521A2 WATER-SOLUBLE FLUORESCENT FULLERENE DERIVATIVE, AND PREPARATION METHOD THEREOF 审中-公开
标题翻译：水溶性氟化物衍生物及其制备方法
公开(公告)号：WO2011136521A2
公开(公告)日：2011-11-03
申请号：PCT/KR2011002991
申请日：2011-04-25
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG BONG HYUN , JEONG JIN YOUNG
发明人： CHUNG BONG HYUN , JEONG JIN YOUNG
IPC分类号： C09K11/06
CPC分类号： C07C41/06 , B82Y30/00 , C07C2604/00 , C09K11/06 , C09K2211/1011 , Y10S977/74 , Y10S977/847 , C07C43/196
摘要： The present invention relates to a water-soluble fluorescent fullerene derivative, and a preparation method thereof, and more specifically, to a method for preparing a water-soluble fluorescent fullerene derivative by mixing a fullerene and a ligand containing a hydroxyl group at end in a first solvent and reacting the mixture with a catalyst, thereby simply preparing a fullerene derivative with excellent fluorescence, and the water-soluble fluorescent fullerene derivative prepared thereby. It is possible to simply prepare a water-soluble fullerene derivative with strong fluorescence by using the preparation method of a water-soluble fluorescent fullerene derivative according to the present invention, to easily control the intensity and wavelength of fluorescent light according to the amount of fullerene and the type of catalyst, and to easily use the fullerene derivative as a biological fluorescent dye by containing a ligand with bioaffinity. The prepared fluorescent fullerene derivative is fluorescent and has excellent solubility in solvents, and thus can be useful in the biological, medical or nanotechnology field and the like.
摘要翻译：本发明涉及一种水溶性荧光富勒烯衍生物及其制备方法，更具体地说，涉及一种通过将富勒烯和末端具有羟基的配体混合而制备水溶性荧光富勒烯衍生物的方法第一溶剂并使混合物与催化剂反应，从而简单地制备具有优异荧光的富勒烯衍生物，由此制备的水溶性荧光富勒烯衍生物。可以通过使用本发明的水溶性荧光富勒烯衍生物的制备方法简单地制备具有强荧光的水溶性富勒烯衍生物，以容易地根据富勒烯的量控制荧光的强度和波长和催化剂的类型，并且通过含有具有生物亲和性的配体容易地使用富勒烯衍生物作为生物荧光染料。制备的荧光富勒烯衍生物是荧光的并且在溶剂中的溶解性优异，因此可用于生物，医学或纳米技术领域等。

4. 发明申请

WO2011031025A3 METHOD FOR DETECTING AND QUANTIFYING A TARGET SUBSTANCE USING A BIOCHIP 审中-公开
标题翻译：用生物薄片检测和定量目标物质的方法
公开(公告)号：WO2011031025A3
公开(公告)日：2011-07-07
申请号：PCT/KR2010005785
申请日：2010-08-27
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG BONG HYUN , KIM SANG KYU , CHO HYUN MIN
发明人： CHUNG BONG HYUN , KIM SANG KYU , CHO HYUN MIN
IPC分类号： G01N33/68 , G01N33/50
CPC分类号： C12Q1/6837 , C12Q2565/113 , C12Q2563/155 , C12Q2563/137 , C12Q2523/31
摘要： The present invention relates to a method for detecting and quantifying a target substance using a biochip having a substrate onto which probe molecules are fixed, and more particularly, to a method which involves preparing a biochip having a substrate onto which probe molecules are fixed, and enabling the biochip to contact a specimen containing a target substance having electric charges, conducting a reaction between the target substance and nanoparticles having electric charges that are opposite to those of the target substance, and conducting a reaction with a metal enhancing solution such that the sizes of the nanoparticles are amplified and the target substance can be detected and quantified using the biochip which can be seen by the naked eye.
摘要翻译：本发明涉及使用具有固定有探针分子的基体的生物芯片来检测和定量目标物质的方法，更具体地涉及制备具有固定探针分子的基体的生物芯片的方法，以及使生物芯片与包含具有电荷的目标物质的样本接触，使目标物质与具有与目标物质的电荷相反的电荷的纳米粒子之间发生反应，并且与金属增强溶液进行反应，使得尺寸的纳米颗粒被扩增，并且可以使用肉眼可见的生物芯片来检测和定量目标物质。

5. 发明申请

WO2009134091A3 METHOD FOR MANUFACTURING UNIFORM SIZE POLYMERIC NANOPARTICLES CONTAINING POORLY SOLUBLE DRUGS 审中-公开
标题翻译：制造含有不溶性药物的均匀聚合物纳米颗粒的方法
公开(公告)号：WO2009134091A3
公开(公告)日：2010-02-11
申请号：PCT/KR2009002289
申请日：2009-04-30
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG BONG HYUN , LIM YONG TAIK , HAN JUNG HYUN
发明人： CHUNG BONG HYUN , LIM YONG TAIK , HAN JUNG HYUN
IPC分类号： B82B3/00
CPC分类号： A61K9/1652 , A61K9/5153 , A61K31/235 , A61K31/343 , A61K31/513 , A61K31/519 , A61K31/5415 , A61K31/573 , A61K31/585 , A61K31/704 , A61K31/7048 , B01D65/00 , C08J3/03 , Y10S516/929
摘要： The present invention relates to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, and more particularly, to a method for manufacturing uniform size polymeric nanoparticles containing poorly soluble drugs, including a first step of dissolving a biodegradable polymer in a non-volatile polar organic solvent, a second step of adding poorly soluble drugs to water and the biodegradable polymer solution to produce a dispersion, and a third step of adding the dispersion to emulsifier solutions in a batch under the condition of low mechanical energy level stirring. The polymeric nanoparticles of the present invention is capable of manufacturing nano-sized small and uniform polymeric nanoparticles through a simple method of employing a non-volatile polar solvent, especially a solvent having a polarity similar to that of water, as a solvent for a mixture solution of biodegradable polymer and poorly soluble materials, and using a low mechanical energy condition and batch-adding of dispersion in an emulsifying process. The polymeric nanoparticles of the present invention are advantageous in that the dissolution rate of the poorly soluble drugs contained in polymeric particles is dramatically improved, and the poorly soluble drugs are gradually and steadily released and maintained at a constant density over a long period of time.
摘要翻译：本发明涉及一种制备均匀尺寸的含有难溶性药物的聚合物纳米颗粒的方法，更具体地说，涉及一种制备均匀尺寸的含有难溶性药物的聚合物纳米颗粒的方法，包括将生物可降解聚合物溶解在非挥发性物质中的第一步骤极性有机溶剂，向水中添加难溶性药物和生物降解性聚合物溶液以产生分散体的第二步骤，以及在低机械能级搅拌的条件下将分散体分批加入到乳化剂溶液中的第三步骤。本发明的聚合物纳米颗粒能够通过使用非挥发性极性溶剂，特别是极性类似于水的溶剂的简单方法作为混合物的溶剂来制造纳米尺寸的小且均匀的聚合物纳米颗粒可生物降解聚合物和难溶物质的溶液，并使用低机械能条件和在乳化过程中分批添加分散体。本发明的聚合物纳米颗粒的优点在于，包含在聚合物颗粒中的难溶性药物的溶解速度显着提高，并且难溶性药物在长时间内逐渐稳定地释放并保持在恒定的密度。

6. 发明申请

WO2009128610A3 CELL LABELING AND IMAGING USING MULTIFUNCTIONAL PERFLUOROCARBON NANOEMULSION 审中-公开
标题翻译：细胞标记和成像使用多功能全氟碳纳米管
公开(公告)号：WO2009128610A3
公开(公告)日：2010-01-07
申请号：PCT/KR2009001488
申请日：2009-03-24
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG BONG HYUN , LIM YONG TAIK
发明人： CHUNG BONG HYUN , LIM YONG TAIK
IPC分类号： G01N33/532 , A61K49/00 , A61K51/00
CPC分类号： G01N33/532 , B82Y15/00 , G01N33/5005 , G01N33/582 , G01N33/587 , G01N33/588
摘要： The present invention relates to cell labeling and imaging using a multifunctional perfluorocarbon (PFC) nanoemulsion. In more detail, the perfluorocarbon nanoemulsion containing optically characteristic nanoparticles is provided with both optical characteristics and 19F magnetic resonance characteristics, and is thus capable of carrying out magnetic resonance imaging (MRI) and optical imaging (OI) at the same time. Cell labeling and imaging using a perfluorocarbon nanoemulsion containing quantum dot nanoparticles exerts no influence on cell viability. The above-described perfluorocarbon nanoemulsion is multifunctional and can be valuably and effectively used in cell labeling and imaging.
摘要翻译：本发明涉及使用多功能全氟化碳（PFC）纳米乳液的细胞标记和成像。更具体地说，含有光学特性纳米粒子的全氟化碳纳米乳剂具有光学特性和19F磁共振特性，因此能够同时进行磁共振成像（MRI）和光学成像（OI）。使用含有量子点纳米颗粒的全氟化碳纳米乳液进行细胞标记和成像对细胞活力没有影响。上述全氟化碳纳米乳液是多功能的，并且可以有价值地有效地用于细胞标记和成像。

7. 发明申请

WO2009154425A9 MULTIMODAL IMAGING METHOD USING NANO-EMULSION COMPRISING OPTICAL NANO-PARTICLES AND PERFLUOROCARBONS 审中-公开
公开(公告)号：WO2009154425A9
公开(公告)日：2009-12-23
申请号：PCT/KR2009/003308
申请日：2009-06-19
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , LIM, Yong Taik
发明人： CHUNG, Bong Hyun , LIM, Yong Taik
IPC分类号： A61K49/06
摘要： The present invention relates to a multimodal imaging method using a nano-emulsion comprising optical nano-particles and perfluorocarbons, and more particularly, to a method of obtaining multimodal images using a nano-emulsion consisted of perfluorocarbons for multispectral magnetic resonance imaging (MRI) and nano-particles for multicolor fluorescent detection. In the multimodal imaging method, various multispectral MRI images and multicolor fluorescent rays can be obtained by varing the kind and combination of perfluorocarbons and the kind and combination of optical nano-particles, so that multiplexed analysis is possible. Accordingly, the multimodal imaging method can be applied to various fields, including biological and medical fields in which studies on cell molecular imaging has been conducted.

8. 发明申请

WO2009120049A3 MULTICHANNEL STRIP FOR A BIOSENSOR 审中-公开
标题翻译：生物传感器的多通道条
公开(公告)号：WO2009120049A3
公开(公告)日：2009-12-17
申请号：PCT/KR2009001612
申请日：2009-03-30
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG BONG HYUN , YOU JUNG MIN , PARK HYE JUNG , KIM SANG KYU
发明人： CHUNG BONG HYUN , YOU JUNG MIN , PARK HYE JUNG , KIM SANG KYU
IPC分类号： G01N35/00
CPC分类号： G01N33/525 , B01L3/508 , B01L2300/0636 , G01N33/54366
摘要： The invention relates to a multichannel strip for a biosensor, comprising: a lower substrate provided with a plurality of reaction portions, each having a defined region; and an upper substrate which comes in contact with the top of each of the plurality of reaction portions and is provided with a plurality of sample inlets and air outlets. The multichannel strip may be effectively used for the assay of various samples and for the diagnosis of all kinds of diseases or the like because it can analyze a number of materials concurrently, has a simple configuration, is easy to produce and can receive a small amount of sample without any pretreatment.
摘要翻译：本发明涉及一种用于生物传感器的多通道条带，包括：下基板，设置有多个反应部分，每个反应部分具有限定的区域; 以及与上述多个反应部的顶部接触的上部基板，设有多个试样入口和空气出口。多通道条可以有效地用于各种样品的测定和各种疾病的诊断等，因为它可以同时分析多种材料，具有简单的结构，易于生产并且可以接收少量的样品没有任何预处理。

9. 发明申请

WO2009044985A1 CASCADE ENZYME-LINKED IMMUNOSORBENT ASSAY 审中-公开
标题翻译： CASCADE ENZYME连接免疫测定
公开(公告)号：WO2009044985A1
公开(公告)日：2009-04-09
申请号：PCT/KR2008/003067
申请日：2008-05-30
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Sang Jeon , LEE, Young-mi , JEONG, Yu-Jin , KANG, Hyo Jin , CHUNG, Bong Hyun
发明人： CHUNG, Sang Jeon , LEE, Young-mi , JEONG, Yu-Jin , KANG, Hyo Jin , CHUNG, Bong Hyun
IPC分类号： G01N33/53 , G01N33/68
CPC分类号： G01N33/54326 , G01N33/581 , G01N33/587 , G01N2333/976
摘要： The present invention relates to a cascade enzyme- linked immunosorbent assay, more precisely a cascade enzyme-linked immunosorbent assay using magnetic microparticles (MMPs) immobilized with the target antigen specific primary antibody and silica nanoparticles (SPs) immobilized with a cascade reaction initiator and the antigen-specific secondary antibody. When the method of the present invention is applied in the detection of an antigen in biosamples, the detection sensitivity can be significantly increased.
摘要翻译：本发明涉及级联酶联免疫吸附测定法，更确切地说，使用固定有靶抗原特异性一级抗体的磁性微粒（MMP）和固定有级联反应引发剂的二氧化硅纳米颗粒（SP）的级联酶联免疫吸附测定法，抗原特异性二抗。当将本发明的方法应用于生物样品中的抗原检测时，可以显着提高检测灵敏度。

10. 发明申请

WO2009014404A3 MULTIFUNCTIONAL PROTEIN SIMULTANEOUSLY DELIVERING ANTIBODIES AND NANOPARTICLES 审中-公开
标题翻译：多功能蛋白同时递送抗体和纳米颗粒
公开(公告)号：WO2009014404A3
公开(公告)日：2009-04-02
申请号：PCT/KR2008004376
申请日：2008-07-25
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG BONG HYUN , LIM YONG TAIK , CHO MI YOUNG , LEE JEONG MIN
发明人： CHUNG BONG HYUN , LIM YONG TAIK , CHO MI YOUNG , LEE JEONG MIN
IPC分类号： C07K19/00
CPC分类号： C07K14/31 , C07K2319/03 , C07K2319/09
摘要： The present invention relates to a polypeptide structure which can simultaneously deliver an antibody and a nanoparticle into cells, more specifically, to a polypeptide structure for intracellular delivery of an antibody and a nanoparticle, which comprises a nanoparticle-binding region, an antibody-binding region and a signaling capable of delivering substances into cells.
摘要翻译：本发明涉及能够将抗体和纳米粒子同时递送到细胞中的多肽结构，更具体地说，涉及用于细胞内递送抗体和纳米粒子的多肽结构，其包含纳米粒子结合区域，抗体结合区域和能够将物质输送到细胞中的信号。

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