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1. 发明申请

WO2006093380A1 THE EXPRESSION AND PURIFICATION METHOD OF HUMAN PROTEIN TYROSINE PHOSPHATASE USING E.COLI SYSTEM 审中-公开
标题翻译：人类蛋白质酪氨酸磷酸酶的表达和纯化方法
公开(公告)号：WO2006093380A1
公开(公告)日：2006-09-08
申请号：PCT/KR2006/000641
申请日：2006-02-24
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , RYU, Seong Eon , CHUNG, Sang Jeon , SON, Jeong Hee , KANG, Hyo Jin , KIM, Sun Kyung , KIM, Jae-hoon , KIM, Seung Jun , JEONG, Dae Gwin , WOO, Eui Jeon , CHO, Yoon-hae
发明人： RYU, Seong Eon , CHUNG, Sang Jeon , SON, Jeong Hee , KANG, Hyo Jin , KIM, Sun Kyung , KIM, Jae-hoon , KIM, Seung Jun , JEONG, Dae Gwin , WOO, Eui Jeon , CHO, Yoon-hae
IPC分类号： C12N15/70
CPC分类号： C12N15/72 , C12N9/16
摘要： The present invention relates to a preparation method of human protein tyrosine phosphatase (PTP) by using E. coli system, more precisely, a method for producing human PTP as an active form in E. coli using various vectors. The human PTPs produced by the method of the present invention can be effectively used for the production of a PTP antibody and/or for the screening of a drug for PTP related diseases.
摘要翻译：本发明涉及使用大肠杆菌系统的人蛋白质酪氨酸磷酸酶（PTP）的制备方法，更准确地说，涉及使用各种载体在大肠杆菌中生产人PTP作为活性形式的方法。通过本发明的方法生产的人PTP可以有效地用于制备PTP抗体和/或用于筛选PTP相关疾病的药物。

2. 发明申请

WO2009054608A1 WATER SOLUBLE PHOTOCHROMIC COMPOUNDS FOR LABELING OF BIOMOLECULES AND METHOD FOR DETECTING BIOMOLECULES USING THE SAME 审中-公开
标题翻译：用于生物分子标记的水溶性光致变色化合物和使用该分析物检测生物分子的方法
公开(公告)号：WO2009054608A1
公开(公告)日：2009-04-30
申请号：PCT/KR2008/004882
申请日：2008-08-21
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , CHUNG, Sang Jeon , LEE, Suh Hyun , CHUNG, Im Sik , PARK, Hyun Kyu , LEE, Chang Soo
发明人： CHUNG, Bong Hyun , CHUNG, Sang Jeon , LEE, Suh Hyun , CHUNG, Im Sik , PARK, Hyun Kyu , LEE, Chang Soo
IPC分类号： A61K49/00
CPC分类号： G01N33/533 , A61K49/0021 , A61K49/006 , C09B11/24
摘要： The present invention relates to water-soluble photochromic compounds for labeling biomolecules and a method of detecting biomolecules using the same. More specifically, relates to water-soluble photochromic compounds for labeling biomolecules, in which a functional group rendering photochromic molecules water-soluble is linked to a functional group capable of binding to biomolecules, and a method of detecting biomolecules using the same. Because the disclosed water-soluble photochromic compounds exhibit the color corresponding to the wavelength of visible light range, they allow signals to be easily detected not only with an UV-VIS spectrophotometer but also visually. Accordingly, the photochromic compounds can be advantageously used in sensors for diagnosing diseases.
摘要翻译：本发明涉及用于标记生物分子的水溶性光致变色化合物和使用其的生物分子的检测方法。更具体地，涉及用于标记生物分子的水溶性光致变色化合物，其中使可溶于水的光致变色分子的官能团与能够结合生物分子的官能团连接，以及使用该生物分子检测生物分子的方法。由于所公开的水溶性光致变色化合物表现出与可见光范围波长相对应的颜色，所以它们不仅可以使用UV-VIS分光光度计而且可视地容易地检测信号。因此，光致变色化合物可有利地用于诊断疾病的传感器中。

3. 发明申请

WO2009044988A1 METHOD FOR LARGE SCALE PREPARATION OF PROCASPASES, WHICH CAN BE ARTIFICIALLY ACTIVATED, IN E. COLI EXPRESSION SYSTEM AND THEIR ACTIVATION 审中-公开
标题翻译：在大肠杆菌表达系统及其活化中大规模制备可被人工激活的方法
公开(公告)号：WO2009044988A1
公开(公告)日：2009-04-09
申请号：PCT/KR2008/003589
申请日：2008-06-24
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Sang Jeon , KANG, Hyo Jin , LEE, Young-mi , KIM, Moonil
发明人： CHUNG, Sang Jeon , KANG, Hyo Jin , LEE, Young-mi , KIM, Moonil
IPC分类号： C12N9/50
CPC分类号： C12N9/50
摘要： The present invention relates to a recombinant caspase and a method for preparing the same, more precisely the present invention relates to a recombinant caspase expression vector capable of being over-expressed without cytotoxicity because the auto-activation recognition site of caspase is replaced with a non-cysteine protease recognition site to nullify the auto-activation activity during the mass-expression in E. coli. Caspase mass- produced from the auto-activation prevented recombinant caspase precursor of the present invention can be effectively used for the development of a novel drug and for studies on biological functions of enzymes.
摘要翻译：本发明涉及一种重组胱天蛋白酶及其制备方法，更确切地说，本发明涉及一种能够由于caspase的自身激活识别位点被非天然氨基酸替换而能够过度表达而不具有细胞毒性的重组胱天蛋白酶表达载体 - 半胱氨酸蛋白酶识别位点，以消除大肠杆菌质量表达期间的自身活化活性。从自动激活产生的胱天蛋白酶阻止了本发明的重组胱天蛋白酶前体可以有效地用于开发新药和研究酶的生物学功能。

4. 发明申请

WO2009044949A1 METHOD FOR PREPARING ANTIBODY MONOLAYERS WHICH HAVE CONTROLLED ORIENTATION USING PEPTIDE HYBRID 审中-公开
标题翻译：使用肽混合物制备具有控制方向的抗体单体的方法
公开(公告)号：WO2009044949A1
公开(公告)日：2009-04-09
申请号：PCT/KR2007/005106
申请日：2007-10-18
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Sang Jeon , CHUNG, Bong Hyun , JUNG, Yongwon , KANG, Hyo Jin , LEE, Jeong Min
发明人： CHUNG, Sang Jeon , CHUNG, Bong Hyun , JUNG, Yongwon , KANG, Hyo Jin , LEE, Jeong Min
IPC分类号： C07K17/00
CPC分类号： C07K17/00 , C07K7/02 , C07K7/08 , G01N33/54353 , G01N33/54393
摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinitiy. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.
摘要翻译：本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说，用于蛋白质固定的肽杂交体，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性蛋白质类型被设计为在固体底物和固定化蛋白之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。用于本发明的蛋白质固定化的肽杂合物有助于控制抗体在各种固体表面上的取向并固定不同来源的各种抗体或具有不同亲和力的不同同种型。因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

5. 发明申请

WO2009072726A1 METHOD FOR LARGE SCALE PREPARATION OF THE ACTIVE DOMAIN OF HUMAN PROTEIN TYROSINE PHOSPHATASE WITHOUT FUSION PROTEIN 审中-公开
标题翻译：不含融合蛋白的人蛋白酪氨酸磷酸酶活性域的大规模制备方法
公开(公告)号：WO2009072726A1
公开(公告)日：2009-06-11
申请号：PCT/KR2008/004524
申请日：2008-08-04
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , RYU, Seong Eon , JEONG, Dae Gwin , KIM, jae Hoon , KIM, Seung Jun , CHUNG, Sang Jeon , SON, Jeong Hee
发明人： RYU, Seong Eon , JEONG, Dae Gwin , KIM, jae Hoon , KIM, Seung Jun , CHUNG, Sang Jeon , SON, Jeong Hee
IPC分类号： C12N15/09
CPC分类号： C12Q1/42 , C12N9/16
摘要： The present invention relates to protein tyrosine phosphatase (PTP) and a method for preparing the same, precisely, a method for expressing PTP active domain with high activity and stability without help of a fusion protein, by using computer based protein structure prediction technique. PTP prepared by the method of the present invention can be effectively used as a protein for high efficiency drug screening for the development of a novel drug, as an antigen protein for the construction of a selective antibody and as a protein for the studies of PTP structure and functions.
摘要翻译：本发明涉及蛋白酪氨酸磷酸酶（PTP）及其制备方法，准确地说，通过使用基于计算机的蛋白质结构预测技术，在没有融合蛋白的帮助下，高活性和稳定表达PTP活性结构域的方法。通过本发明方法制备的PTP可以有效地用作用于高效药物筛选的蛋白质，用于开发新型药物，作为构建选择性抗体的抗原蛋白和用于研究PTP结构的蛋白质和功能。

6. 发明申请

WO2009044985A1 CASCADE ENZYME-LINKED IMMUNOSORBENT ASSAY 审中-公开
标题翻译： CASCADE ENZYME连接免疫测定
公开(公告)号：WO2009044985A1
公开(公告)日：2009-04-09
申请号：PCT/KR2008/003067
申请日：2008-05-30
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Sang Jeon , LEE, Young-mi , JEONG, Yu-Jin , KANG, Hyo Jin , CHUNG, Bong Hyun
发明人： CHUNG, Sang Jeon , LEE, Young-mi , JEONG, Yu-Jin , KANG, Hyo Jin , CHUNG, Bong Hyun
IPC分类号： G01N33/53 , G01N33/68
CPC分类号： G01N33/54326 , G01N33/581 , G01N33/587 , G01N2333/976
摘要： The present invention relates to a cascade enzyme- linked immunosorbent assay, more precisely a cascade enzyme-linked immunosorbent assay using magnetic microparticles (MMPs) immobilized with the target antigen specific primary antibody and silica nanoparticles (SPs) immobilized with a cascade reaction initiator and the antigen-specific secondary antibody. When the method of the present invention is applied in the detection of an antigen in biosamples, the detection sensitivity can be significantly increased.
摘要翻译：本发明涉及级联酶联免疫吸附测定法，更确切地说，使用固定有靶抗原特异性一级抗体的磁性微粒（MMP）和固定有级联反应引发剂的二氧化硅纳米颗粒（SP）的级联酶联免疫吸附测定法，抗原特异性二抗。当将本发明的方法应用于生物样品中的抗原检测时，可以显着提高检测灵敏度。

7. 发明申请

WO2012176977A3 MICROSCOPE APPARATUS FOR DETECTING OR IMAGING PROTEIN USING IFRET PROBE, AND METHOD FOR DETECTING OR IMAGING PROTEIN USING SAME 审中-公开
标题翻译：用于使用IFRET探针检测或成像蛋白质的微阵列装置，以及使用其检测或成像蛋白质的方法
公开(公告)号：WO2012176977A3
公开(公告)日：2013-02-14
申请号：PCT/KR2012003126
申请日：2012-04-23
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG SANG JEON , KANG HYO JIN , KIM JU HWAN
发明人： CHUNG SANG JEON , KANG HYO JIN , KIM JU HWAN
IPC分类号： G01N33/483 , G01N21/64 , G01N33/68
CPC分类号： G01N21/6428 , G01N21/6458 , G01N33/542 , G01N33/6803 , G01N2021/6441
摘要： A microscope apparatus for detecting or imaging a target protein using an induced fluorescence resonance energy transfer (iFRET) probe, according to the present invention, comprises: a light irradiation portion for irradiating a first light within a wavelength which excites an amino acid inside the target protein, and a second light within a wavelength which excites a fluorescent molecule in the iFRET probe; an objective lens for incidenting the light which is irradiated from the light irradiation portion on a sample into which the iFRET probe is introduced; and a recognition portion for detecting a first light emission signal, which is generated from the iFRET probe by irradiating on the sample the first light within the wavelength that excites the amino acid inside the target protein, and a second light emission signal, which is generated from the iFRET probe by irradiating the second light within the wavelength that excites the fluorescent molecule in the iFRET probe, wherein the iFRET probe is comprised by binding directly or through a linker a target protein-specific binding site or a molecule having the binding site, and a fluorescent molecule having an acceptor function to the intrinsic fluorescence of the target protein.
摘要翻译：根据本发明的使用感应荧光共振能量转移（iFRET）探针检测或成像靶蛋白的显微镜装置包括：光照射部分，用于照射激发靶内的氨基酸的波长内的第一光蛋白质和在iFRET探针中激发荧光分子的波长内的第二光; 用于将从光照射部分照射的光入射到引入了iFRET探针的样品上的物镜; 以及识别部分，用于检测从iFRET探针产生的第一发光信号，通过在样品上照射激发靶蛋白内的氨基酸的波长内的第一光和产生的第二发光信号通过在iFRET探针中激发荧光分子的波长内照射第二个光，通过iFRET探针直接或通过接头结合靶蛋白特异性结合位点或具有结合位点的分子，以及对靶蛋白的固有荧光具有受体功能的荧光分子。

8. 发明申请

WO2012177106A3 PROBE FOR IFRET AND USAGE OF SAME 审中-公开
标题翻译：探索和使用它们
公开(公告)号：WO2012177106A3
公开(公告)日：2013-04-11
申请号：PCT/KR2012005009
申请日：2012-06-25
申请人： KOREA RES INST OF BIOSCIENCE , CHUNG SANG JEON , KIM JU HWAN , RUCHKINA ELENA , KANG HYO JIN
发明人： CHUNG SANG JEON , KIM JU HWAN , RUCHKINA ELENA , KANG HYO JIN
IPC分类号： G01N33/52 , C09K11/06 , G01N21/64 , G01N33/53 , G01N33/68
CPC分类号： G01N33/582 , G01N21/6428 , G01N33/542 , G01N33/573 , G01N2021/6441 , G01N2458/00
摘要： The present invention relates to a probe for iFRET and to a usage of same. More particularly, the present invention relates to the probe for iFRET, a method for manufacturing the probe for iFRET, a method for searching a target protein-specific binding site or a molecule having the binding site using the probe for iFRET, and a method for imaging the target protein through the probe for iFRET. The probe for iFRET, according to the present invention, unlike the existing FRET method, uses an amino acid inside a protein as a fluorescence donor, thereby using only one fluorescent substance, and has an light emitting wavelength which is separated from a protein intrinsic fluorescence, thereby having high specificity and sensitivity and thus allowing easier and more accurate analysis of the amount, activity, and mechanism, among others, of a variety of proteins.
摘要翻译：本发明涉及一种用于iFRET的探针及其用途。更具体地，本发明涉及用于iFRET的探针，用于制造用于iFRET的探针的方法，使用所述iFRET探针来搜索靶蛋白特异性结合位点或具有所述结合位点的分子的方法，以及用于通过iFRET的探针成像靶蛋白。根据本发明的用于iFRET的探针与现有的FRET方法不同，使用蛋白质内部的氨基酸作为荧光供体，从而仅使用一种荧光物质，并且具有与蛋白质固有荧光分离的发光波长，因此具有高特异性和灵敏度，因此允许更容易且更准确地分析各种蛋白质的量，活性和机制等。

9. 发明申请

WO2007132998A1 LINKER MOLECULES FOR SUBSTRATE SURFACE TREATMENT AND SPECIFIC PROTEIN IMMOBILIZATION, AND METHOD FOR PREPARING THE SAME 审中-公开
标题翻译：用于基板表面处理和特定蛋白质固定的连接分子及其制备方法
公开(公告)号：WO2007132998A1
公开(公告)日：2007-11-22
申请号：PCT/KR2007/002250
申请日：2007-05-08
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Bong Hyun , HA, Tai Hwan , JUNG, Sun Ok , CHUNG, Sang Jeon , LEE, Suh Hyun , KIM, Jung-Won
发明人： CHUNG, Bong Hyun , HA, Tai Hwan , JUNG, Sun Ok , CHUNG, Sang Jeon , LEE, Suh Hyun , KIM, Jung-Won
IPC分类号： C07K19/00
CPC分类号： C07K19/00
摘要： The present invention relates to a linker molecule for immobilizing a protein on a substrate surface and a preparation method thereof. More specifically, relates to a linker molecule, which has a functional group binding to a substrate, and a functional group binding to a protein, at both ends of a protein-immobilizing ligand, respectively, as well as a preparation method thereof and a method for immobilizing a protein through the linker molecule. The disclosed linker molecule can form a self-assembled monolayer on a substrate selected from the group consisting of gold, silver, semiconductor, glass, silicon and polymer, so as to minimize non-specific binding in a process of immobilizing a protein on the substrate. Accordingly, the linker molecule will be useful for biochips, biosensors and other supports for protein immobilization.
摘要翻译：本发明涉及用于将蛋白质固定在基材表面上的连接分子及其制备方法。更具体地，涉及分别具有与底物结合的官能团的接头分子和与蛋白质固定化配体两端的蛋白质结合的官能团，以及其制备方法和方法用于通过连接分子固定蛋白质。所公开的接头分子可以在选自金，银，半导体，玻璃，硅和聚合物的基底上形成自组装单层，以便在将蛋白质固定在基底上的过程中使非特异性结合最小化。因此，连接分子可用于生物芯片，生物传感器和用于蛋白质固定的其它载体。

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