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1. 发明申请

WO2009044985A1 CASCADE ENZYME-LINKED IMMUNOSORBENT ASSAY 审中-公开
标题翻译： CASCADE ENZYME连接免疫测定
公开(公告)号：WO2009044985A1
公开(公告)日：2009-04-09
申请号：PCT/KR2008/003067
申请日：2008-05-30
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Sang Jeon , LEE, Young-mi , JEONG, Yu-Jin , KANG, Hyo Jin , CHUNG, Bong Hyun
发明人： CHUNG, Sang Jeon , LEE, Young-mi , JEONG, Yu-Jin , KANG, Hyo Jin , CHUNG, Bong Hyun
IPC分类号： G01N33/53 , G01N33/68
CPC分类号： G01N33/54326 , G01N33/581 , G01N33/587 , G01N2333/976
摘要： The present invention relates to a cascade enzyme- linked immunosorbent assay, more precisely a cascade enzyme-linked immunosorbent assay using magnetic microparticles (MMPs) immobilized with the target antigen specific primary antibody and silica nanoparticles (SPs) immobilized with a cascade reaction initiator and the antigen-specific secondary antibody. When the method of the present invention is applied in the detection of an antigen in biosamples, the detection sensitivity can be significantly increased.
摘要翻译：本发明涉及级联酶联免疫吸附测定法，更确切地说，使用固定有靶抗原特异性一级抗体的磁性微粒（MMP）和固定有级联反应引发剂的二氧化硅纳米颗粒（SP）的级联酶联免疫吸附测定法，抗原特异性二抗。当将本发明的方法应用于生物样品中的抗原检测时，可以显着提高检测灵敏度。

2. 发明申请

WO2009044949A1 METHOD FOR PREPARING ANTIBODY MONOLAYERS WHICH HAVE CONTROLLED ORIENTATION USING PEPTIDE HYBRID 审中-公开
标题翻译：使用肽混合物制备具有控制方向的抗体单体的方法
公开(公告)号：WO2009044949A1
公开(公告)日：2009-04-09
申请号：PCT/KR2007/005106
申请日：2007-10-18
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Sang Jeon , CHUNG, Bong Hyun , JUNG, Yongwon , KANG, Hyo Jin , LEE, Jeong Min
发明人： CHUNG, Sang Jeon , CHUNG, Bong Hyun , JUNG, Yongwon , KANG, Hyo Jin , LEE, Jeong Min
IPC分类号： C07K17/00
CPC分类号： C07K17/00 , C07K7/02 , C07K7/08 , G01N33/54353 , G01N33/54393
摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinitiy. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.
摘要翻译：本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说，用于蛋白质固定的肽杂交体，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性蛋白质类型被设计为在固体底物和固定化蛋白之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。用于本发明的蛋白质固定化的肽杂合物有助于控制抗体在各种固体表面上的取向并固定不同来源的各种抗体或具有不同亲和力的不同同种型。因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

3. 发明申请

WO2009044988A1 METHOD FOR LARGE SCALE PREPARATION OF PROCASPASES, WHICH CAN BE ARTIFICIALLY ACTIVATED, IN E. COLI EXPRESSION SYSTEM AND THEIR ACTIVATION 审中-公开
标题翻译：在大肠杆菌表达系统及其活化中大规模制备可被人工激活的方法
公开(公告)号：WO2009044988A1
公开(公告)日：2009-04-09
申请号：PCT/KR2008/003589
申请日：2008-06-24
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , CHUNG, Sang Jeon , KANG, Hyo Jin , LEE, Young-mi , KIM, Moonil
发明人： CHUNG, Sang Jeon , KANG, Hyo Jin , LEE, Young-mi , KIM, Moonil
IPC分类号： C12N9/50
CPC分类号： C12N9/50
摘要： The present invention relates to a recombinant caspase and a method for preparing the same, more precisely the present invention relates to a recombinant caspase expression vector capable of being over-expressed without cytotoxicity because the auto-activation recognition site of caspase is replaced with a non-cysteine protease recognition site to nullify the auto-activation activity during the mass-expression in E. coli. Caspase mass- produced from the auto-activation prevented recombinant caspase precursor of the present invention can be effectively used for the development of a novel drug and for studies on biological functions of enzymes.
摘要翻译：本发明涉及一种重组胱天蛋白酶及其制备方法，更确切地说，本发明涉及一种能够由于caspase的自身激活识别位点被非天然氨基酸替换而能够过度表达而不具有细胞毒性的重组胱天蛋白酶表达载体 - 半胱氨酸蛋白酶识别位点，以消除大肠杆菌质量表达期间的自身活化活性。从自动激活产生的胱天蛋白酶阻止了本发明的重组胱天蛋白酶前体可以有效地用于开发新药和研究酶的生物学功能。

4. 发明申请

WO2006093380A1 THE EXPRESSION AND PURIFICATION METHOD OF HUMAN PROTEIN TYROSINE PHOSPHATASE USING E.COLI SYSTEM 审中-公开
标题翻译：人类蛋白质酪氨酸磷酸酶的表达和纯化方法
公开(公告)号：WO2006093380A1
公开(公告)日：2006-09-08
申请号：PCT/KR2006/000641
申请日：2006-02-24
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , RYU, Seong Eon , CHUNG, Sang Jeon , SON, Jeong Hee , KANG, Hyo Jin , KIM, Sun Kyung , KIM, Jae-hoon , KIM, Seung Jun , JEONG, Dae Gwin , WOO, Eui Jeon , CHO, Yoon-hae
发明人： RYU, Seong Eon , CHUNG, Sang Jeon , SON, Jeong Hee , KANG, Hyo Jin , KIM, Sun Kyung , KIM, Jae-hoon , KIM, Seung Jun , JEONG, Dae Gwin , WOO, Eui Jeon , CHO, Yoon-hae
IPC分类号： C12N15/70
CPC分类号： C12N15/72 , C12N9/16
摘要： The present invention relates to a preparation method of human protein tyrosine phosphatase (PTP) by using E. coli system, more precisely, a method for producing human PTP as an active form in E. coli using various vectors. The human PTPs produced by the method of the present invention can be effectively used for the production of a PTP antibody and/or for the screening of a drug for PTP related diseases.
摘要翻译：本发明涉及使用大肠杆菌系统的人蛋白质酪氨酸磷酸酶（PTP）的制备方法，更准确地说，涉及使用各种载体在大肠杆菌中生产人PTP作为活性形式的方法。通过本发明的方法生产的人PTP可以有效地用于制备PTP抗体和/或用于筛选PTP相关疾病的药物。

5. 发明公开

EP2205641A1 METHOD FOR PREPARING ANTIBODY MONOLAYERS WHICH HAVE CONTROLLED ORIENTATION USING PEPTIDE HYBRID 有权
标题翻译：过程产生抗体能力MONO层，控取向以肽HYBRIDS
公开(公告)号：EP2205641A1
公开(公告)日：2010-07-14
申请号：EP07833415.8
申请日：2007-10-18
申请人： Korea Research Institute of Bioscience and Biotechnology
发明人： CHUNG, Sang Jeon , CHUNG, Bong Hyun , JUNG, Yongwon , KANG, Hyo Jin , LEE, Jeong Min
IPC分类号： C07K17/00
CPC分类号： C07K17/00 , C07K7/02 , C07K7/08 , G01N33/54353 , G01N33/54393
摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinitiy. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.

6. 发明公开

EP2725356A2 MICROSCOPE APPARATUS FOR DETECTING OR IMAGING PROTEIN USING IFRET PROBE, AND METHOD FOR DETECTING OR IMAGING PROTEIN USING SAME 审中-公开
标题翻译：显微镜装置获认可或蛋白质的插图一个IFRET探针和方法的身材和PROTEINERKENNUNGS-
公开(公告)号：EP2725356A2
公开(公告)日：2014-04-30
申请号：EP12802701.8
申请日：2012-04-23
申请人： Korea Research Institute Of Bioscience And Biotechnology
发明人： CHUNG, Sang Jeon , KANG, Hyo Jin , KIM, Ju Hwan
IPC分类号： G01N33/483 , G01N21/64 , G01N33/68
CPC分类号： G01N21/6428 , G01N21/6458 , G01N33/542 , G01N33/6803 , G01N2021/6441
摘要： A microscope apparatus for detecting or imaging a target protein using a probe for intrinsic fluorescence resonance energy transfer (iFRET) according to the present invention comprises: a light irradiation unit that irradiates a first light having a wavelength range for exciting an amino acid in the target protein and a second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET; an objective lens that allows the lights irradiated from the light irradiation unit to be incident onto a sample into which the probe for iFRET is introduced; and a recognition unit that detects a first light emitting signal generated from the probe for iFRET by irradiating the first light having a wavelength range for exciting an amino acid in the target protein onto the sample and a second light emitting signal generated from the probe for iFRET by irradiating the second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET onto the sample, wherein the probe for iFRET includes: a binding site specific to a target protein or a molecule which has the binding site; and a fluorescent molecule having an acceptor function with respect to intrinsic fluorescence of the target protein, which are bonded to each other directly or by a linker.
摘要翻译：用于检测或成像使用固有荧光共振能量转移（iFRET）雅丁到本发明的样品的靶蛋白的显微镜装置，包括：光照射部做照射具有用于激发的波长范围内的第一光在氨基酸中的目标蛋白，并具有用于所述样品的iFRET激发荧光分子的波长范围的第二光; 物镜的确实允许从光照射部照射的光能够入射到样品引入其中用于iFRET被引入所述样品; 和识别单元做了检测第一光通过照射具有在靶蛋白氨基酸的波长范围用于激发到样品上，并从所述探针用于iFRET产生的第二发光信号的第一发光从探头对iFRET生成的信号通过照射具有用于样品iFRET到样品的激发荧光分子的波长范围内的第二光，对于worin iFRET的样品包括：特定于靶蛋白或具有结合位点的分子的结合位点; 和荧光分子具有左到受体功能相对于所述靶蛋白，其直接或通过接合到海誓山盟的固有荧光。

7. 发明公开

EP2193372A1 CASCADE ENZYME-LINKED IMMUNOSORBENT ASSAY 有权
标题翻译： CASCADES酶联免疫吸附试验
公开(公告)号：EP2193372A1
公开(公告)日：2010-06-09
申请号：EP08766029.6
申请日：2008-05-30
申请人： Korea Research Institute of Bioscience and Biotechnology
发明人： CHUNG, Sang Jeon , LEE, Young-mi , JEONG, Yu-Jin , KANG, Hyo Jin , CHUNG, Bong Hyun
IPC分类号： G01N33/53 , G01N33/68
CPC分类号： G01N33/54326 , G01N33/581 , G01N33/587 , G01N2333/976
摘要： The present invention relates to a cascade enzyme- linked immunosorbent assay, more precisely a cascade enzyme-linked immunosorbent assay using magnetic microparticles (MMPs) immobilized with the target antigen specific primary antibody and silica nanoparticles (SPs) immobilized with a cascade reaction initiator and the antigen-specific secondary antibody. When the method of the present invention is applied in the detection of an antigen in biosamples, the detection sensitivity can be significantly increased.

8. 发明授权

EP2205641B1 METHOD FOR PREPARING ANTIBODY MONOLAYERS WHICH HAVE CONTROLLED ORIENTATION USING PEPTIDE HYBRID 有权
标题翻译：过程产生抗体能力MONO层，控取向以肽HYBRIDS
公开(公告)号：EP2205641B1
公开(公告)日：2014-07-09
申请号：EP07833415.8
申请日：2007-10-18
申请人： Korea Research Institute of Bioscience and Biotechnology
发明人： CHUNG, Sang Jeon , CHUNG, Bong Hyun , JUNG, Yongwon , KANG, Hyo Jin , LEE, Jeong Min
IPC分类号： C07K17/00 , G01N33/543 , C07K17/06
CPC分类号： C07K17/00 , C07K7/02 , C07K7/08 , G01N33/54353 , G01N33/54393

9. 发明公开

EP2725357A2 PROBE FOR IFRET AND USAGE OF SAME 审中-公开
标题翻译： IFRET-SONDE UND IHRE VERWENDUNG
公开(公告)号：EP2725357A2
公开(公告)日：2014-04-30
申请号：EP12802627.5
申请日：2012-06-25
申请人： Korea Research Institute Of Bioscience And Biotechnology
发明人： CHUNG, Sang Jeon , KIM, Ju Hwan , RUCHKINA, Elena , KANG, Hyo Jin
IPC分类号： G01N33/52 , G01N33/53 , G01N33/68 , G01N21/64 , C09K11/06
CPC分类号： G01N33/582 , G01N21/6428 , G01N33/542 , G01N33/573 , G01N2021/6441 , G01N2458/00
摘要： The present invention relates to a probe for iFRET and use thereof. Specifically, the present invention relates to a novel probe for iFRET, a method for preparing the probe for iFRET, a method for searching a target protein-specific binding site or a molecule having the binding site using the probe for iFRET, and a method for imaging the target protein using the probe for iFRET. The probe for iFRET according to the present invention utilizes an amino acid in a protein as a fluorescent donor, unlike the conventional FRET method. Therefore, only one fluorescent material is used, and its emission wavelength is distinct from the intrinsic fluorescence of the protein. Thus, high specificity and sensitivity are ensured, and the quantity, activity and mechanism of various proteins can be analyzed in an easy and accurate manner.
摘要翻译：本发明涉及一种用于iFRET的探针及其用途。具体地说，本发明涉及一种用于iFRET的新型探针，一种用于制备iFRET探针的方法，一种使用该探针进行iFRET检测靶蛋白特异性结合位点或具有该结合位点的分子的方法，以及一种使用iFRET的探针成像靶蛋白。根据本发明的iFRET的探针利用蛋白质中的氨基酸作为荧光供体，与常规FRET方法不同。因此，仅使用一种荧光材料，其发射波长与蛋白质的固有荧光不同。因此，确保了高度的特异性和灵敏度，可以容易且准确地分析各种蛋白质的数量，活性和机理。

10. 发明授权

EP2193372B1 CASCADE ENZYME-LINKED IMMUNOSORBENT ASSAY 有权
标题翻译： CASCADES酶联免疫吸附试验
公开(公告)号：EP2193372B1
公开(公告)日：2012-11-21
申请号：EP08766029.6
申请日：2008-05-30
申请人： Korea Research Institute of Bioscience and Biotechnology
发明人： CHUNG, Sang Jeon , LEE, Young-mi , JEONG, Yu-Jin , KANG, Hyo Jin , CHUNG, Bong Hyun
IPC分类号： G01N33/53 , G01N33/68
CPC分类号： G01N33/54326 , G01N33/581 , G01N33/587 , G01N2333/976

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