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71. 发明授权

US07947196B2 Ion exchangeable mixture and method of producing the same 有权
标题翻译：可离子交换混合物及其制备方法
公开(公告)号：US07947196B2
公开(公告)日：2011-05-24
申请号：US11453116
申请日：2006-06-14
申请人： Hun-joo Lee , Ji-na Namgoong , Soo-suk Lee , Joon-ho Kim
发明人： Hun-joo Lee , Ji-na Namgoong , Soo-suk Lee , Joon-ho Kim
IPC分类号： C01B31/16
CPC分类号： C08J5/2218 , B01J39/20 , B01J41/14 , B01J47/016 , B01J47/12 , B01L3/5027 , C08J5/20
摘要： An ion exchangeable mixture containing a polymeric compound consisting of an ion exchange resin, an acrylamide mixture containing at least one bisacrylamide and at least one acrylamide, and a copolymer obtained by reacting the polymeric compound with the acrylamide mixture, and a method of producing the same are provided. The ion exchangeable membrane produced by using the ion exchangeable mixture has significantly smaller electric resistance than conventional ion exchangeable membranes, and has excellent selective permeability because the ion exchangeable membrane is electrically charged. The ion exchangeable membrane can be produced under very mild production conditions, and thus can be produced very easily. Furthermore, the ion exchangeable membrane can be also formed into film during a crosslinking reaction in a solvent of water, and thus is advantageous in that the ion exchangeable membrane can be freely produced into desired sizes, shapes and forms.
摘要翻译：含有由离子交换树脂，含有至少一种双丙烯酰胺和至少一种丙烯酰胺的丙烯酰胺混合物组成的聚合物的离子交换性混合物和通过使聚合物与丙烯酰胺混合物反应得到的共聚物及其制造方法被提供。通过使用离子交换性混合物制造的离子交换膜的电阻比常规的离子交换膜的电阻小得多，并且由于可离子交换膜带电而具有优异的选择性渗透性。离子交换膜可以在非常温和的生产条件下生产，因此可以非常容易地生产。此外，在水的溶剂中的交联反应期间，也可以将离子交换膜形成为膜，因此有利的是可离子交换膜可以自由地制成所需的尺寸，形状和形状。

72. 发明授权

US07790440B2 Sensing switch and detecting method using the same 有权
标题翻译：感应开关及使用其的检测方法
公开(公告)号：US07790440B2
公开(公告)日：2010-09-07
申请号：US12272183
申请日：2008-11-17
申请人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
发明人： Kyu-tae Yoo , Joon-ho Kim , Jun-hong Min , Sung-ouk Jung , Ji-na Namgoong , Kui-hyun Kim , Jeo-young Shim , Kak Namkoong
IPC分类号： C12M3/00
CPC分类号： G01N33/54373
摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.
摘要翻译：提供了一种感测开关和使用该感测开关的感测方法。感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

73. 发明授权

US07771573B2 Microfluidic device for electrochemically regulating pH of fluid therein and method of regulating pH of fluid using the microfluidic device 有权
标题翻译：用于电化学调节流体pH值的微流体装置和使用微流体装置调节流体pH值的方法
公开(公告)号：US07771573B2
公开(公告)日：2010-08-10
申请号：US11489289
申请日：2006-07-19
申请人： Hun-joo Lee , Joon-ho Kim , Chin-sung Park , Young-sun Lee , Jong-Suk Chung
发明人： Hun-joo Lee , Joon-ho Kim , Chin-sung Park , Young-sun Lee , Jong-Suk Chung
IPC分类号： C25B9/00 , C02F1/461
CPC分类号： B01D67/0062 , B01D2311/2623
摘要： Provided is a microfluidic device for electrochemically regulating the pH of a fluid. The microfluidic device includes: an ion-exchange material; an anode chamber having a surface defined by a surface of the ion-exchange material and an anode electrode disposed along an edge of the surface of the anode chamber; and a cathode chamber having a surface defined by a surface of the ion-exchange material and a cathode electrode disposed along an edge of the surface of the cathode chamber, wherein the anode chamber and the cathode chamber are separated by an insulation material.
摘要翻译：提供了用于电化学调节流体pH的微流体装置。微流体装置包括：离子交换材料; 具有由所述离子交换材料的表面限定的表面的阳极室和沿着所述阳极室的表面的边缘设置的阳极电极; 以及阴极室，其具有由离子交换材料的表面限定的表面和沿着阴极室的表面的边缘设置的阴极电极，其中阳极室和阴极室由绝缘材料分隔开。

74. 发明授权

US07736050B2 Fluid mixing device using cross channels 有权
标题翻译：流体混合装置使用交叉通道
公开(公告)号：US07736050B2
公开(公告)日：2010-06-15
申请号：US11433594
申请日：2006-05-12
申请人： Chin-sung Park , Joon-ho Kim , Kak Namkoong , Kwang-wook Oh
发明人： Chin-sung Park , Joon-ho Kim , Kak Namkoong , Kwang-wook Oh
IPC分类号： B01F5/00
CPC分类号： B01F13/1013 , B01F5/0475 , B01F5/0478 , B01F5/0646 , B01F5/0647 , B01F13/0059 , B01F13/1019 , B01F15/0203 , B01F15/0404 , B01F2015/0221 , G01N1/38
摘要： Provided is a fluid mixing device which produces a series of solutions with a concentration gradient. The fluid mixing device includes: a plurality of first channels disposed parallel to each other on a layer, and into which an equal amount of diluent flows from its upstream; a plurality of second channels formed perpendicular to the first channels on an adjacent layer to the layer on which the first channels are formed, and into which an equal amount of sample solution flows from its upstream; and via holes formed at at least one intersection between each of the first channel and a plurality of second channels so that a predetermined amount of sample solution flows from the second channels into corresponding first channels, wherein a series of solutions with different concentrations is produced in the first channels depending on the amount of sample solution that flows into the first channels through the via holes. Thus, a series of solutions with different concentrations is output from the first channels.
摘要翻译：提供了一种流体混合装置，其产生具有浓度梯度的一系列溶液。流体混合装置包括：在层上彼此平行设置的多个第一通道，等量的稀释剂从其上游流入其中; 多个第二通道，其垂直于与形成有第一通道的层相邻的第一通道形成，并且等量的样品溶液从其上游流入其中; 以及形成在每个第一通道和多个第二通道之间的至少一个相交处的通孔，使得预定量的样品溶液从第二通道流入相应的第一通道，其中一系列具有不同浓度的溶液在第一通道取决于通过通孔流入第一通道的样品溶液的量。因此，从第一通道输出一系列具有不同浓度的溶液。

75. 发明授权

US07579172B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07579172B2
公开(公告)日：2009-08-25
申请号：US11071383
申请日：2005-03-03
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/66 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： An apparatus for amplifying nucleic acids includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate, wherein the reactant aqueous solution contacts the fluid to create a plurality of droplets surrounded by the fluid in the reaction vessel and the nucleic acids are amplified in the reactant aqueous solution droplets.
摘要翻译：用于放大核酸的装置包括：基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许引入含有反应物的反应物水溶液，核酸扩增到反应容器中，形成在基底内的第二入口通道，连接到反应容器的末端，并允许引入与反应物水溶液相分离并且不参与扩增反应的流体反应容器和加热单元，其以与基底热接触并加热基底的方式安装在基底上，其中反应物水溶液接触流体以产生由反应容器中的流体包围的多个液滴，核酸在反应物水溶液液滴中被扩增。

76. 发明申请

US20060118417A1 Nucleic acid purification method using hydrogen bonding and electric field 审中-公开
标题翻译：使用氢键和电场的核酸纯化方法
公开(公告)号：US20060118417A1
公开(公告)日：2006-06-08
申请号：US11281226
申请日：2005-11-16
申请人： Young-a Kim , Jun-hong Min , Kui-hyun Kim , Myo-yong Lee , Su-hyeon Kim , Young-nam Kwon , Jeong-gun Lee , Joon-ho Kim
发明人： Young-a Kim , Jun-hong Min , Kui-hyun Kim , Myo-yong Lee , Su-hyeon Kim , Young-nam Kwon , Jeong-gun Lee , Joon-ho Kim
IPC分类号： C07H21/04
CPC分类号： C07H21/04
摘要： Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.
摘要翻译：提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

77. 发明申请

US20050186602A1 Method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or amino group 审中-公开
标题翻译：使用涂覆有羧基或氨基的固相材料来扩增核酸的方法
公开(公告)号：US20050186602A1
公开(公告)日：2005-08-25
申请号：US11042376
申请日：2005-01-25
申请人： Joon-ho Kim , Yoon-kyoung Cho , Jung-joo Hwang , Geun-bae Lim , Jeong-gun Lee
发明人： Joon-ho Kim , Yoon-kyoung Cho , Jung-joo Hwang , Geun-bae Lim , Jeong-gun Lee
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1006
摘要： A method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or an amino group is provided. The method includes contacting a mixture of a nucleic acid containing sample and a salt solution with the solid phase material coated with a carboxyl group or an amino group to form a nucleic acid-solid phase material complex, washing the nucleic acid-solid phase material complex with a wash buffer, and adding a reaction solution for amplifying a nucleic acid to the nucleic acid-solid phase material complex to perform an amplification reaction.
摘要翻译：提供了使用涂覆有羧基或氨基的固相材料来扩增核酸的方法。该方法包括将含有核酸的样品和盐溶液的混合物与涂覆有羧基或氨基的固相材料接触以形成核 - 固相物质络合物，洗涤核酸 - 固相物质络合物用洗涤缓冲液加入用于将核酸扩增至核酸 - 固相物质络合物的反应溶液进行扩增反应。

78. 发明申请

US20050136463A1 Method for isolating nucleic acid using alumina 审中-公开
标题翻译：使用氧化铝分离核酸的方法
公开(公告)号：US20050136463A1
公开(公告)日：2005-06-23
申请号：US11016447
申请日：2004-12-17
申请人： Joon-ho Kim , Yoon-kyoung Cho , Geun-bae Lim , Soo-hwan Jeong
发明人： Joon-ho Kim , Yoon-kyoung Cho , Geun-bae Lim , Soo-hwan Jeong
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6806 , C12Q2527/137 , C12Q2523/308
摘要： A method for isolating a nucleic acid is provided. The method includes mixing a nucleic acid-containing sample, a salt solution, and alumina; isolating the alumina having the nucleic acid attached thereto from the liquid; washing the alumina having the nucleic acid attached thereto; and eluting the nucleic acid from the alumina.
摘要翻译：提供了分离核酸的方法。该方法包括混合含核酸的样品，盐溶液和氧化铝; 从液体中分离具有与其连接的核酸的氧化铝; 洗涤附着有核酸的氧化铝; 并从氧化铝中洗脱核酸。

79. 发明授权

US06364342B1 Retainer ring having air deflector and airbag mounting structure using the same 有权
标题翻译：具有导风板的保持环和使用其的安全气囊安装结构
公开(公告)号：US06364342B1
公开(公告)日：2002-04-02
申请号：US09478178
申请日：2000-01-05
申请人： Joon-ho Kim
发明人： Joon-ho Kim
IPC分类号： B60R2126
CPC分类号： B60R21/217
摘要： A retainer ring having an air deflector, for use with an airbag mounting structure, includes a flange in which an inflater opening for receiving an inflater is formed; and a wall, a base of which is connected to the flange, extending upward and outward from the inflater opening at a predetermined angle to diffuse and deflect exploding gas upward. The wall, which realizes a simplified configuration for the retainer ring, may include convex portions projecting inwardly towards the inflater opening, being regularly spaced, and rib formations integrally formed on an inner side of the wall between the convex portions. The rib formations are evenly spaced, as a pair of rows covering the height of the inner side of the wall, and slope downward on either side of the convex portions. The airbag mounting structure clamps the inflater and the airbag between the retainer ring and a baseplate, which is mounted to a support within an automobile. To determine a mounting position and orientation, a tabbed cut-out is formed in the retainer ring, juxtaposed to a coupling hole thereof, and is bent at a right angle to pass through an alignment notch formed in a flange of the inflater and a series of alignment holes formed in the other components.
摘要翻译：具有用于气囊安装结构的导风板的保持环包括其中形成有用于接收充气器的充气器开口的凸缘; 并且其底部连接到凸缘的壁，其以预定角度从充气器开口向上和向外延伸，以将爆炸气体向上扩散和偏转。实现保持环的简化构造的壁可以包括向内朝向充气器开口向内突出的凸起部分，其规则地间隔开，并且肋构形一体地形成在凸起部分之间的壁的内侧上。肋骨形成均匀的间隔，作为覆盖壁的内侧的高度的一对排，并且在凸部的任一侧向下倾斜。安全气囊安装结构将充气器和气囊夹在保持环和安装到汽车内的支撑件的基板之间。为了确定安装位置和方向，在保持环中形成有与其联接孔并列的标签切口，并且以直角弯曲以穿过形成在充气器的凸缘中的对准凹口和一系列的对准孔形成在其他部件中。

80. 发明授权

US08945484B2 Microfluidic device including microvalve 有权
标题翻译：微流控器件包括微型阀
公开(公告)号：US08945484B2
公开(公告)日：2015-02-03
申请号：US13188075
申请日：2011-07-21
申请人： Kak Namkoong , Joon-ho Kim , Won-jong Jung , Chin-sung Park
发明人： Kak Namkoong , Joon-ho Kim , Won-jong Jung , Chin-sung Park
IPC分类号： F16K3/00 , B01L3/00 , F16K99/00
CPC分类号： B01L3/502738 , B01L2300/0816 , B01L2300/123 , B01L2400/0655 , F16K37/0066 , F16K99/0015 , F16K99/0057 , F16K99/0059 , F16K2099/0084
摘要： A microfluidic device including at least one microvalve which controls a flow of a fluid, where the at least one microvalve includes: a pneumatic layer, a fluidic layer disposed opposite to the pneumatic layer; an elastomer membrane disposed between the pneumatic layer and the fluidic layer; and an asymmetric valve seat protruding from a surface of the fluidic layer toward a surface of the elastomer membrane and asymmetrically dividing a space between the fluidic layer and the elastomer membrane.
摘要翻译：一种微流体装置，包括控制流体流动的至少一个微型阀，其中所述至少一个微型阀包括：气动层，与所述气动层相对设置的流体层; 设置在气动层和流体层之间的弹性体膜; 以及从流体层的表面朝向弹性体膜的表面突出的不对称的阀座，并且不对称地划分流体层和弹性体膜之间的空间。

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