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1. 发明申请

US20050202489A1 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US20050202489A1
公开(公告)日：2005-09-15
申请号：US11071383
申请日：2005-03-03
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12N15/09 , B01F5/06 , B01F13/00 , B01F15/02 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/38 , C12N15/00 , C12Q1/68 , G01N35/00 , G01N35/08 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： Provided are a method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：提供了用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

2. 发明授权

US07579172B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07579172B2
公开(公告)日：2009-08-25
申请号：US11071383
申请日：2005-03-03
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/66 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： An apparatus for amplifying nucleic acids includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate, wherein the reactant aqueous solution contacts the fluid to create a plurality of droplets surrounded by the fluid in the reaction vessel and the nucleic acids are amplified in the reactant aqueous solution droplets.
摘要翻译：用于放大核酸的装置包括：基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许引入含有反应物的反应物水溶液，核酸扩增到反应容器中，形成在基底内的第二入口通道，连接到反应容器的末端，并允许引入与反应物水溶液相分离并且不参与扩增反应的流体反应容器和加热单元，其以与基底热接触并加热基底的方式安装在基底上，其中反应物水溶液接触流体以产生由反应容器中的流体包围的多个液滴，核酸在反应物水溶液液滴中被扩增。

3. 发明授权

US07943348B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07943348B2
公开(公告)日：2011-05-17
申请号：US12504271
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

4. 发明授权

US07915013B2 Method and apparatus for amplifying nucleic acids 有权
标题翻译：扩增核酸的方法和装置
公开(公告)号：US07915013B2
公开(公告)日：2011-03-29
申请号：US12504213
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： An apparatus for amplifying nucleic acids. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：扩增核酸的装置。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

5. 发明申请

US20100021972A1 METHOD AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS 有权
标题翻译：用于放大核酸的方法和装置
公开(公告)号：US20100021972A1
公开(公告)日：2010-01-28
申请号：US12504271
申请日：2009-07-16
申请人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
发明人： Yoon-kyoung Cho , Joon-ho Kim , Kak Namkoong , Geun-bae Lim , Jun-hong Min
IPC分类号： C12P19/34
CPC分类号： G01N35/08 , B01F5/0646 , B01F5/0647 , B01F5/0654 , B01F13/0071 , B01F2015/0221 , B01J2219/00783 , B01J2219/00824 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/0086 , B01J2219/00873 , B01J2219/00891 , B01L3/5027 , B01L3/502715 , B01L2300/0809 , B01L2300/0861 , B01L2300/0883 , G01N2035/00366 , G01N2035/00514
摘要： A method and apparatus for amplifying nucleic acids. The method includes introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction, creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel, and amplifying the nucleic acids in the reactant aqueous solution droplets. The apparatus includes a substrate, a reaction vessel formed inside of the substrate, at least one first inlet channel formed inside the substrate, connected to an end of the reaction vessel, and allowing introduction of a reactant aqueous solution containing reactants for nucleic acid amplification into the reaction vessel, a second inlet channel formed inside the substrate, connected to the end of the reaction vessel, and allowing introduction of a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction into the reaction vessel, and a heating unit installed on the substrate in such a way to thermally contact with the substrate and heating the substrate.
摘要翻译：用于扩增核酸的方法和装置。该方法包括通过不同的入口通道将含有用于核酸扩增的反应物的反应物水溶液和与反应物水溶液相分离并且不参与扩增反应的流体引入反应容器，形成多个反应物水溶液通过使反应物水溶液与反应容器中的流体接触，并且扩增反应物水溶液液滴中的核酸，由流体包围的液滴。该装置包括基板，形成在基板内部的反应容器，形成在基板内部的至少一个第一入口通道，与反应容器的端部连接，并且允许将含有用于核酸扩增的反应物的反应物水溶液引入到反应容器，形成在基板内部的第二入口通道，连接到反应容器的端部，并且允许引入与反应物水溶液相分离并且不参与扩增反应的流体进入反应容器，以及加热单元，其以与基板热接触并加热基板的方式安装在基板上。

6. 发明申请

US20050158777A1 Method of isolating nucleic acid by using carbon nanotube 审中-公开
标题翻译：使用碳纳米管分离核酸的方法
公开(公告)号：US20050158777A1
公开(公告)日：2005-07-21
申请号：US11024315
申请日：2004-12-28
申请人： Yoon-kyoung Cho , Joon-ho Kim , Jung-joo Hwang , Wan-jun Park , Geun-bae Lim
发明人： Yoon-kyoung Cho , Joon-ho Kim , Jung-joo Hwang , Wan-jun Park , Geun-bae Lim
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/04
CPC分类号： B82Y30/00 , C12N15/1006 , C12Q1/6806 , C12Q2563/155
摘要： A method of isolating nucleic acid using a carbon nanotube is provided. The method includes contacting a mixture of a sample containing nucleic acid and a salt solution with a carbon nanotube to form a nucleic acid-carbon nanotube composite; washing the nucleic acid-carbon nanotube composite with a washing buffer; and eluting the nucleic acid from the nucleic acid-carbon nanotube composite.
摘要翻译：提供了使用碳纳米管分离核酸的方法。该方法包括将含有核酸和盐溶液的样品与碳纳米管的混合物接触以形成核酸 - 碳纳米管复合物; 用洗涤缓冲液洗涤核酸 - 碳纳米管复合物; 并从核酸 - 碳纳米管复合物中洗脱核酸。

7. 发明申请

US20050186602A1 Method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or amino group 审中-公开
标题翻译：使用涂覆有羧基或氨基的固相材料来扩增核酸的方法
公开(公告)号：US20050186602A1
公开(公告)日：2005-08-25
申请号：US11042376
申请日：2005-01-25
申请人： Joon-ho Kim , Yoon-kyoung Cho , Jung-joo Hwang , Geun-bae Lim , Jeong-gun Lee
发明人： Joon-ho Kim , Yoon-kyoung Cho , Jung-joo Hwang , Geun-bae Lim , Jeong-gun Lee
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1006
摘要： A method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or an amino group is provided. The method includes contacting a mixture of a nucleic acid containing sample and a salt solution with the solid phase material coated with a carboxyl group or an amino group to form a nucleic acid-solid phase material complex, washing the nucleic acid-solid phase material complex with a wash buffer, and adding a reaction solution for amplifying a nucleic acid to the nucleic acid-solid phase material complex to perform an amplification reaction.
摘要翻译：提供了使用涂覆有羧基或氨基的固相材料来扩增核酸的方法。该方法包括将含有核酸的样品和盐溶液的混合物与涂覆有羧基或氨基的固相材料接触以形成核 - 固相物质络合物，洗涤核酸 - 固相物质络合物用洗涤缓冲液加入用于将核酸扩增至核酸 - 固相物质络合物的反应溶液进行扩增反应。

8. 发明申请

US20050136463A1 Method for isolating nucleic acid using alumina 审中-公开
标题翻译：使用氧化铝分离核酸的方法
公开(公告)号：US20050136463A1
公开(公告)日：2005-06-23
申请号：US11016447
申请日：2004-12-17
申请人： Joon-ho Kim , Yoon-kyoung Cho , Geun-bae Lim , Soo-hwan Jeong
发明人： Joon-ho Kim , Yoon-kyoung Cho , Geun-bae Lim , Soo-hwan Jeong
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6806 , C12Q2527/137 , C12Q2523/308
摘要： A method for isolating a nucleic acid is provided. The method includes mixing a nucleic acid-containing sample, a salt solution, and alumina; isolating the alumina having the nucleic acid attached thereto from the liquid; washing the alumina having the nucleic acid attached thereto; and eluting the nucleic acid from the alumina.
摘要翻译：提供了分离核酸的方法。该方法包括混合含核酸的样品，盐溶液和氧化铝; 从液体中分离具有与其连接的核酸的氧化铝; 洗涤附着有核酸的氧化铝; 并从氧化铝中洗脱核酸。

9. 发明授权

US07799557B2 Polymerase chain reaction (PCR) module and multiple PCR system using the same 有权
标题翻译：聚合酶链反应（PCR）模块和多重PCR系统使用相同
公开(公告)号：US07799557B2
公开(公告)日：2010-09-21
申请号：US11080705
申请日：2005-03-15
申请人： Kwang-wook Oh , Jin-tae Kim , Kak Namkoong , Chin-sung Park , Yoon-kyoung Cho
发明人： Kwang-wook Oh , Jin-tae Kim , Kak Namkoong , Chin-sung Park , Yoon-kyoung Cho
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N21/6486 , B01L3/50851 , B01L7/52 , B01L2300/0627 , B01L2300/18 , G01N21/0332
摘要： Provided are a DNA PCR module and a multiple PCR system using the same. More particularly, provided are a DNA PCR module with a combined PCR thermal cycler and PCR product detector, and a multiple PCR system using the same.
摘要翻译：提供了DNA PCR模块和使用其的多重PCR系统。更具体地，提供了具有组合的PCR热循环仪和PCR产物检测器的DNA PCR模块，以及使用其的多重PCR系统。

10. 发明申请

US20060140051A1 Microfluidic device including microchannel on which plurality of electromagnets are disposed, and methods of mixing sample and lysing cells using the microfluidic device 审中-公开
标题翻译：微流体装置包括其上设置有多个电磁体的微通道，以及使用微流体装置混合样品和裂解细胞的方法
公开(公告)号：US20060140051A1
公开(公告)日：2006-06-29
申请号：US11285924
申请日：2005-11-22
申请人： Jin-tae Kim , Kwang-wook Oh , Yoon-kyoung Cho , Sang-hyun Peak , Sook-young Kim , Chin-sung Park , Kak Namkoong
发明人： Jin-tae Kim , Kwang-wook Oh , Yoon-kyoung Cho , Sang-hyun Peak , Sook-young Kim , Chin-sung Park , Kak Namkoong
IPC分类号： B01F13/08
CPC分类号： C12N1/066 , B01F13/0059 , B01F13/0809
摘要： Provided is a microfluidic device including at least one inlet, at least one outlet, and a microchannel connecting the inlet and the outlet. The microfluidic device includes two or more electromagnets disposed on sidewalls of the microchannel and oriented in a predetermined direction with respect to the direction in which the microchannel extends.
摘要翻译：提供了一种微流体装置，其包括连接入口和出口的至少一个入口，至少一个出口和微通道。微流体装置包括设置在微通道的侧壁上并相对于微通道延伸的方向在预定方向上定向的两个或多个电磁体。

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