专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20060118417A1 Nucleic acid purification method using hydrogen bonding and electric field 审中-公开
标题翻译：使用氢键和电场的核酸纯化方法
公开(公告)号：US20060118417A1
公开(公告)日：2006-06-08
申请号：US11281226
申请日：2005-11-16
申请人： Young-a Kim , Jun-hong Min , Kui-hyun Kim , Myo-yong Lee , Su-hyeon Kim , Young-nam Kwon , Jeong-gun Lee , Joon-ho Kim
发明人： Young-a Kim , Jun-hong Min , Kui-hyun Kim , Myo-yong Lee , Su-hyeon Kim , Young-nam Kwon , Jeong-gun Lee , Joon-ho Kim
IPC分类号： C07H21/04
CPC分类号： C07H21/04
摘要： Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.
摘要翻译：提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

2. 发明授权

US07855069B2 Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
标题翻译：使用微磁珠和激光快速破坏细胞或病毒的方法和装置
公开(公告)号：US07855069B2
公开(公告)日：2010-12-21
申请号：US11253541
申请日：2005-10-19
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
IPC分类号： C12M1/42 , C12M1/33
CPC分类号： C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
摘要翻译：提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题裂解芯片可应用于LOC。

3. 发明申请

US20060110725A1 Apparatus for and method of purifying nucleic acids by different laser absorption of beads 审中-公开
标题翻译：通过不同激光吸收珠粒来纯化核酸的装置和方法
公开(公告)号：US20060110725A1
公开(公告)日：2006-05-25
申请号：US11190169
申请日：2005-07-26
申请人： Jeong-gun Lee , Young-nam Kwon , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Young-a Kim
发明人： Jeong-gun Lee , Young-nam Kwon , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Young-a Kim
IPC分类号： C12Q1/70 , C12Q1/68 , C12N7/02 , C12N1/08 , C12M1/34
CPC分类号： B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0647 , B01L2200/10 , B01L2300/0681 , B01L2300/0867 , B01L2300/087 , B01L2400/0487
摘要： An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples, magnetic beads, and solid support in the capillary; a laser generator attached to the capillary and irradiating a laser beam onto the capillary; a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall; a waste chamber attached to the capillary and discharging a lysate; an elution buffer chamber attached to the capillary and eluting nucleic acids from the solid support having nucleic acids bound thereto; and a neutralization buffer chamber attached to the capillary and supplying a neutralization buffer for neutralizing the eluted nucleic acid solution. According to the apparatus and method, PCR inhibitors can be removed to increase PCR yield and nucleic acids can be purified using a silicon substrate or silica beads. Thus, the apparatus and method can be applied to LOC fabrication.
摘要翻译：提供了一种纯化细胞或病毒核酸的装置和方法。核酸纯化装置包括：细胞裂解毛细管，其具有样品入口，通过所述样品入口引入样品，磁珠和固体支持物; 连接到毛细管并将样品，磁珠和固体支持物混合在毛细管中的振动器; 激光发生器附接到毛细管并将激光束照射到毛细管上; 附着到毛细管并将磁珠固定到毛细管壁的磁力发生器; 连接到毛细管并排出裂解物的废物室; 洗脱缓冲室连接到毛细管并从具有与其结合的核酸的固体支持物洗脱核酸; 以及中和缓冲室，其连接到毛细管并提供用于中和洗脱的核酸溶液的中和缓冲液。根据该装置和方法，可以除去PCR抑制剂以增加PCR产量，并且可以使用硅底物或二氧化硅珠纯化核酸。因此，该装置和方法可以应用于LOC制造。

4. 发明申请

US20060084165A1 Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
标题翻译：使用微磁珠和激光快速破坏细胞或病毒的方法和装置
公开(公告)号：US20060084165A1
公开(公告)日：2006-04-20
申请号：US11253541
申请日：2005-10-19
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
IPC分类号： C12N1/06
CPC分类号： C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
摘要翻译：提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决裂解芯片可应用于LOC。

5. 发明申请

US20060199199A1 Method of removing nucleic acid amplification inhibitor from biological sample and PCR system 审中-公开
标题翻译：从生物样品和PCR系统中去除核酸扩增抑制剂的方法
公开(公告)号：US20060199199A1
公开(公告)日：2006-09-07
申请号：US11336568
申请日：2006-01-20
申请人： Kui-hyun Kim , Young-a Kim , Jun-hong Min , Jeong-gun Lee , In-ho Lee
发明人： Kui-hyun Kim , Young-a Kim , Jun-hong Min , Jeong-gun Lee , In-ho Lee
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34
CPC分类号： C12Q1/6848 , B01L3/5027 , C12Q2563/149 , C12Q2527/125
摘要： Provided is a method of removing a nucleic acid amplification inhibitor from a biological sample. The method includes contacting the biological sample to a carboxyl group-coated solid support. Provided is also a micro-PCR system including a sample pretreatment chamber including a carboxyl group-coated solid support; a PCR chamber; and a channel connecting the sample pretreatment chamber and the PCR chamber.
摘要翻译：提供从生物样品中除去核酸扩增抑制剂的方法。该方法包括使生物样品与羧基涂覆的固体支持物接触。还提供了一种微型PCR系统，其包括含有羧基的固体支持物的样品预处理室; PCR室; 以及连接样品预处理室和PCR室的通道。

6. 发明授权

US07429470B2 Method for purification of nucleic acids by phase separation using laser and beads 有权
标题翻译：使用激光和珠子通过相分离纯化核酸的方法
公开(公告)号：US07429470B2
公开(公告)日：2008-09-30
申请号：US11265461
申请日：2005-11-02
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim
IPC分类号： C12N13/00 , C12N1/08 , G01N33/00 , C07H21/02
CPC分类号： B01L3/502761 , B01L3/502738 , B01L7/52 , B01L2200/0668 , B01L2300/0838 , B01L2300/087 , B01L2400/0633 , G01N35/0098 , Y10T436/143333
摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.
摘要翻译：提供了用于纯化细胞或病毒核酸的装置和方法。核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混合在毛细管中的振动器; 激光发生器，附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。使用电磁铁确保去除PCR抑制剂。此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。

7. 发明申请

US20060094051A1 Apparatus and method for purification of nucleic acids by phase separation using laser and beads 有权
标题翻译：使用激光和珠子通过相分离纯化核酸的装置和方法
公开(公告)号：US20060094051A1
公开(公告)日：2006-05-04
申请号：US11265461
申请日：2005-11-02
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim
IPC分类号： C12Q1/68 , C12N1/08 , C12M1/34
CPC分类号： B01L3/502761 , B01L3/502738 , B01L7/52 , B01L2200/0668 , B01L2300/0838 , B01L2300/087 , B01L2400/0633 , G01N35/0098 , Y10T436/143333
摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.
摘要翻译：提供了用于纯化细胞或病毒核酸的装置和方法。核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混合在毛细管中的振动器; 激光发生器附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。使用电磁铁确保去除PCR抑制剂。此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。

8. 发明授权

US07531138B2 Device and method for rapidly lysing cells or viruses 失效
标题翻译：用于快速裂解细胞或病毒的装置和方法
公开(公告)号：US07531138B2
公开(公告)日：2009-05-12
申请号：US11560890
申请日：2006-11-17
申请人： Su-hyeon Kim , Jeong-gun Lee
发明人： Su-hyeon Kim , Jeong-gun Lee
IPC分类号： B01L3/00 , G01N1/00 , C12M1/00
CPC分类号： C12M47/06 , B01L3/5027 , C12N1/066 , C12N13/00 , G01N1/44 , Y10T137/2496 , Y10T436/25
摘要： A cell lysis device for lysing cells or viruses, comprising a cell lysis tube having a sample inlet; a pump connected to the cell lysis tube for transferring a sample into the tube; a sealing unit for reversibly sealing a specific region of the tube; and a laser source for generating a laser is provided. Further, a method of lysing cells or viruses using the cell lysis device is provided. The method comprises introducing a sample containing cells or viruses and optionally magnetic beads to the cell lysis tube through the sample inlet; transferring the sample to a specific region in the cell lysis tube by means of the pump; temporarily sealing the region of the cell lysis tube where the sample is placed with the sealing unit; irradiating the sample with the laser; removing the sealing unit from the cell lysis tube; and discharging the sample from the cell lysis tube by means of the pump.
摘要翻译：一种用于裂解细胞或病毒的细胞裂解装置，包括具有样品入口的细胞裂解管; 连接到细胞裂解管的泵，用于将样品转移到管中; 用于可逆地密封管的特定区域的密封单元; 并提供用于产生激光的激光源。此外，提供了使用细胞裂解装置裂解细胞或病毒的方法。该方法包括通过样品入口将含有细胞或病毒的样品和任选的磁珠引入细胞裂解管; 通过泵将样品转移到细胞裂解管中的特定区域; 用密封单元临时密封放置样品的细胞溶解管的区域; 用激光照射样品; 从细胞裂解管中取出密封单元; 并通过泵从细胞裂解管排出样品。

9. 发明授权

US07943088B2 Biochemical analyzer and method of controlling internal temperature of the biochemical analyzer 有权
标题翻译：生化分析仪和生化分析仪内部温度控制方法
公开(公告)号：US07943088B2
公开(公告)日：2011-05-17
申请号：US11972708
申请日：2008-01-11
申请人： Jeong-gun Lee , Yong-moo Shin , Kui-hyun Kim , Jin-han Chung
发明人： Jeong-gun Lee , Yong-moo Shin , Kui-hyun Kim , Jin-han Chung
IPC分类号： C12Q1/68 , G01N15/06
CPC分类号： B01L7/52 , B01L3/5027 , B01L9/527 , B01L2300/0681 , B01L2300/0806 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2300/1883 , B01L2400/0409 , G01N35/00069 , G01N2035/00346 , Y10T436/11 , Y10T436/25
摘要： Provided is a biochemical analyzer in which a microfluidic biochemical assay may be performed. The analyzer includes: a microfluidic device loading space including a microfluidic device supporting unit detachably supporting a microfluidic device including an energy application region in which an energy is applied; an energy source loading space including an energy source applying the energy to the radiation application region; and an isolation wall isolating the microfluidic device loading space and the energy source loading space to prevent heat transfer between the microfluidic device loading space and the energy source loading space and including a window through which the energy can be transmitted. A method of controlling an internal temperature of the biochemical analyzer is also provided.
摘要翻译：提供了可以进行微流体生化测定的生化分析仪。该分析仪包括：微流体装置装载空间，其包括微流体装置支撑单元，其可拆卸地支撑包括其中施加能量的能量施加区域的微流体装置; 能量源装载空间，其包括将能量施加到所述辐射施加区域的能量源; 隔离壁隔离微流体装置加载空间和能量源装载空间，以防止微流体装置加载空间与能量源装载空间之间的热传递并且包括能够传输能量的窗口。还提供了一种控制生化分析仪内部温度的方法。

10. 发明授权

US08367422B2 Biochemical analyzer and method of controlling internal temperature of the biochemical analyzer 有权
标题翻译：生化分析仪和生化分析仪内部温度控制方法
公开(公告)号：US08367422B2
公开(公告)日：2013-02-05
申请号：US13081193
申请日：2011-04-06
申请人： Jeong-gun Lee , Yong-moo Shin , Kui-hyun Kim , Jin-han Chung
发明人： Jeong-gun Lee , Yong-moo Shin , Kui-hyun Kim , Jin-han Chung
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48
CPC分类号： B01L7/52 , B01L3/5027 , B01L9/527 , B01L2300/0681 , B01L2300/0806 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2300/1883 , B01L2400/0409 , G01N35/00069 , G01N2035/00346 , Y10T436/11 , Y10T436/25
摘要： Provided is a biochemical analyzer including: a microfluidic device loading space including a microfluidic device supporting unit detachably supporting a microfluidic device including an electromagnetic radiation application region in which electromagnetic energy is applied; an energy source loading space including an energy source applying the electromagnetic energy to the electromagnetic radiation application region; and an isolation wall isolating the microfluidic device loading space and the energy source loading space to prevent heat transfer between the microfluidic device loading space and the energy source loading space and including a transparent window through which the electromagnetic energy can be transmitted. A method of controlling an internal temperature of the biochemical analyzer is also provided.
摘要翻译：提供了一种生物化学分析仪，其包括：微流体装置装载空间，包括微流体装置支撑单元，其可拆卸地支撑包括施加电磁能的电磁辐射施加区域的微流体装置; 能量源装载空间，其包括将电磁能施加到电磁辐射施加区域的能量源; 以及隔离壁，隔离微流体装置加载空间和能量源装载空间，以防止微流体装置加载空间和能量源装载空间之间的热传递，并且包括可透射电磁能的透明窗口。还提供了一种控制生化分析仪内部温度的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式