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21. 发明申请

US20100124777A1 Bacterium producing L-glutamic acid and method for producing L-glutamic acid 审中-公开
标题翻译：产生L-谷氨酸的细菌和L-谷氨酸的生产方法
公开(公告)号：US20100124777A1
公开(公告)日：2010-05-20
申请号：US11785643
申请日：2007-04-19
申请人： Hiromi Ohtaki , Jun Nakamura , Hiroshi Izui , Tsuyoshi Nakamatsu
发明人： Hiromi Ohtaki , Jun Nakamura , Hiroshi Izui , Tsuyoshi Nakamatsu
IPC分类号： C12N1/21 , C07H21/04
CPC分类号： C12N9/1051 , C12N9/90 , C12P13/14 , C12R1/13 , C12R1/15
摘要： L-Glutamic acid is produced by culturing a coryneform bacterium having L-glutamic acid producing ability, in which trehalose synthesis ability is decreased or deleted by, for example, disrupting a gene coding for trehalose-6-phosphate synthase, a gene coding for maltooligosyltrehalose synthase, or both of these genes to produce and accumulate L-glutamic acid in the medium, and collecting the L-glutamic acid from the medium.
摘要翻译： L-谷氨酸是通过培养具有L-谷氨酸生产能力的棒状细菌产生的，其中海藻糖合成能力通过例如破坏编码海藻糖-6-磷酸合酶的基因减少或缺失，编码麦芽糖糖基海藻糖的基因合成酶或这两种基因，以在培养基中产生和积累L-谷氨酸，并从培养基中收集L-谷氨酸。

22. 发明授权

US07432085B2 Method for producing L-amino acid 有权
标题翻译：生产L-氨基酸的方法
公开(公告)号：US07432085B2
公开(公告)日：2008-10-07
申请号：US11281495
申请日：2005-11-18
申请人： Yoshihiko Hara , Hiroshi Izui , Takahiro Asano , Yasuyuki Watanabe , Tsuyoshi Nakamatsu
发明人： Yoshihiko Hara , Hiroshi Izui , Takahiro Asano , Yasuyuki Watanabe , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C12P13/06 , C12P13/16 , C12P13/20 , C12P13/24
CPC分类号： C12P13/04 , C12N9/88 , C12P13/14
摘要： In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.
摘要翻译：通过培养具有在培养基中产生L-氨基酸的能力的微生物来生产L-氨基酸的方法，以在培养基中产生和积累L-氨基酸并从培养基中收集L-氨基酸，具有Entner-Doudoroff途径并被修饰的革兰氏阴性细菌被用作微生物，使得6-磷酸葡萄糖酸脱水酶活性或2-酮-3-脱氧-6-磷酸葡萄糖酸醛缩酶活性或两者的活性增强。

23. 发明授权

US07351571B2 Process for the production of β-amino acids using acylase 有权
标题翻译：使用酰基转移酶生产β-氨基酸的方法
公开(公告)号：US07351571B2
公开(公告)日：2008-04-01
申请号：US11198242
申请日：2005-08-08
申请人： Tsuyoshi Nakamatsu , Hisashi Kawasaki , Kunihiko Watanabe , Masakazu Nakazawa , Kunisuke Izawa
发明人： Tsuyoshi Nakamatsu , Hisashi Kawasaki , Kunihiko Watanabe , Masakazu Nakazawa , Kunisuke Izawa
IPC分类号： C12N1/20 , C12P13/04 , C07H21/04
CPC分类号： C12P13/04 , C12P41/006 , C12R1/01
摘要： The present invention relates to a Variovorax sp. which produces an acylase having an asymmetric hydrolysis activity on an N-acetyl β-amino acid to selectively produce an R-β-amino acid, and a Burkholderia sp. which produces both an acylase having an asymmetric hydrolysis activity on an N-acetyl β-amino acid to selectively produce an S-β-amino acid and an acylase having an asymmetric hydrolysis activity of an N-acetyl β-amino acid to selectively produce an R-β-amino acid, and a process for the selective production of an S-, or R-β-amino acid using the above strains.
摘要翻译：本发明涉及一种Variovorax sp。其产生对N-乙酰基β-氨基酸具有不对称水解活性以选择性地产生R-β-氨基酸的酰基转移酶和伯克霍尔德氏菌属（Burkholderia sp。其产生对N-乙酰基β-氨基酸具有不对称水解活性的酰基转移酶以选择性地产生具有N-乙酰基β-氨基酸的不对称水解活性的S-β-氨基酸和酰基转移酶，以选择性地产生 R-β-氨基酸，以及使用上述菌株选择性生产S-或R-β-氨基酸的方法。

24. 发明授权

US07297521B2 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
标题翻译：棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
公开(公告)号：US07297521B2
公开(公告)日：2007-11-20
申请号：US11011701
申请日：2004-12-15
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12P13/10
CPC分类号： C12N9/93 , C12P13/10
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译：编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽序列表所示的SEQ ID NO：2中的50〜393的酸值，（b）至少具有序列表所示的序列号2所示的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代，一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰磷酸合成酶活性的蛋白质，其具有氨基酸序列SEQ ID NO：3的氨基甲酰磷酸合成酶的大亚基，（c ）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，（d）具有序列表所示的SEQ ID NO：3的氨基酸序列的多肽，包括取代，缺失，插入，添加，或反转一个或几个氨基酸，并可构成具有氨基酸 - 磷酸合成酶活性的蛋白质，其具有SEQ ID NO：2中氨基酸序列号为50〜393的氨基酸序列的氨基甲酰磷酸合成酶的小亚基。

25. 发明授权

US06881566B2 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
标题翻译：棒状细菌的氨基甲酰磷酸合成酶基因及L-精氨酸的制备方法
公开(公告)号：US06881566B2
公开(公告)日：2005-04-19
申请号：US09836470
申请日：2001-04-18
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12N9/00 , C12P13/10 , C12N1/20 , C07H21/04
CPC分类号： C12P13/10 , C12N9/93
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID No: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence comprising at least the amino acid numbers 55 to 1113 of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.
摘要翻译：编码以下（a）或（b）中定义的多肽的DNA片段和以下（c）或（d）中定义的多肽：（a）至少具有氨基酸的氨基酸序列的多肽序列表所示的SEQ ID NO：2中的酸值为50〜393，（b）至少具有序列表所示的SEQ ID No：2的氨基酸序列号为50〜393的氨基酸序列的多肽，包括取代，一个或几个氨基酸的缺失，插入，添加或倒位，并且可以构成具有氨基甲酰基 - 磷酸合成酶活性的蛋白质，其具有氨基酸 - 磷酸合成酶的大亚基，其氨基酸序列至少包含氨基酸数55至 SEQ ID NO：3的1113，（c）具有至少包含序列表所示的SEQ ID NO：3的氨基酸编号55至1113的氨基酸序列的多肽，（d）具有氨基酸的多肽序列至少包含氨基酸编号55至11 包括序列表中所示的SEQ ID NO：3的13个氨基酸残基，其包含一个或多个氨基酸的取代，缺失，插入，添加或倒位，并且可以构成具有氨甲酰磷酸合成酶活性的氨基甲酰磷酸合成酶的小亚基具有SEQ ID NO：2中氨基酸序列号50至393的氨基酸序列。

26. 发明授权

US07858348B2 N-acetyl-(R,S)-beta-amino acid acylase gene 有权
标题翻译： N-乙酰基 - （R，S） - 氨基酸酰基转移酶基因
公开(公告)号：US07858348B2
公开(公告)日：2010-12-28
申请号：US11782260
申请日：2007-07-24
申请人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
发明人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04
CPC分类号： C12N9/80
摘要： The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.
摘要翻译：本发明提供编码N-乙酰基 - （R，S） - 氨基酸酰基转移酶的基因。从细菌细胞中分离纯化N-乙酰基 - （R，S） - 氨基酸酰基转移酶，测定核苷酸序列。宿主如大肠杆菌用于构建这些基因的高表达系统。由伯克霍尔德氏菌属（Burkholderia sp。）生产的N-乙酰基 - （R） - AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。由伯克霍尔德氏菌属（Burkholderia sp。）生产的N-乙酰基 - （S） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 9.由Variovorax sp。生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110348（FERM BP-10367）包括例如由SEQ ID NO：1所示的氨基酸序列组成的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 11。

27. 发明授权

US07608437B2 Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
标题翻译：构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法
公开(公告)号：US07608437B2
公开(公告)日：2009-10-27
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/14 , C12Q1/48 , C12Q1/32 , C12Q1/68 , C12N9/02 , C12N9/10 , C12N1/21 , C12P21/00 , C12N15/00 , C07K14/00 , C07H21/00
CPC分类号： C12P13/14 , C12N15/67
摘要： A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.
摘要翻译：具有改进的氨基酸或核酸生产力的棒状杆菌细菌的方法包括以下步骤：将氨基酸或核酸 - 生物合成基因的启动子序列中的突变引入棒状细菌的染色体上，使其接近共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使得其接近共有序列，获得棒状杆菌型氨基酸的突变体或产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。该方法允许本领域技术人员通过重组或突变来构建能够富集或控制预期基因的表达而不使用质粒并以高产率促进氨基酸产生的突变体。

28. 发明申请

US20080241895A1 N-Acetyl-(R,S)-beta-Amino Acid Acylase Gene 有权
标题翻译： N-乙酰基 - （R，S） - 氨基酸酰基酶
公开(公告)号：US20080241895A1
公开(公告)日：2008-10-02
申请号：US11782260
申请日：2007-07-24
申请人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
发明人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C07H21/04 , C12N9/80 , C12N1/21
CPC分类号： C12N9/80
摘要： The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.
摘要翻译：本发明提供编码N-乙酰基 - （R，S） - 氨基酸酰基转移酶的基因。从细菌细胞中分离纯化N-乙酰基 - （R，S） - 氨基酸酰基转移酶，确定核苷酸序列。宿主如大肠杆菌用于构建这些基因的高表达系统。伯克霍尔德氏菌属生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。由伯克霍尔德氏菌属生产的N-乙酰基 - （S） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 9.由Variovorax sp。生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110348（FERM BP-10367）包括例如由SEQ ID NO：1所示的氨基酸序列组成的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 11。

29. 发明申请

US20050095680A1 Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine 有权
公开(公告)号：US20050095680A1
公开(公告)日：2005-05-05
申请号：US11011701
申请日：2004-12-15
申请人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
发明人： Yoko Kuwabara , Kenichi Hashiguchi , Tsuyoshi Nakamatsu , Osamu Kurahashi , Yukiko Mori , Hisao Ito
IPC分类号： C12N1/21 , C12N9/00 , C12P13/10 , C07H21/04 , C12N9/12
CPC分类号： C12N9/93 , C12P13/10
摘要： A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d): (a) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing, (b) a polypeptide which has at least the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a large subunit of carbamoyl-phosphate synthetase having the amino acid sequence of SEQ ID NO: 3, (c) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (d) a polypeptide which has the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and can constitute a protein having a carbamoyl-phosphate synthetase activity with a small subunit of carbamoyl-phosphate synthetase having the amino acid sequence of the amino acid numbers 50 to 393 in SEQ ID NO: 2.

30. 发明授权

US06338956B1 Stress-resistant microorganism and method of producing fermentative product 有权
标题翻译：耐应力微生物和发酵产物的生产方法
公开(公告)号：US06338956B1
公开(公告)日：2002-01-15
申请号：US09634632
申请日：2000-08-08
申请人： Eiichiro Kimura , Yoshimi Kikuchi , Yoshio Kawahara , Shinya Goto , Osamu Kurahashi , Tsuyoshi Nakamatsu
发明人： Eiichiro Kimura , Yoshimi Kikuchi , Yoshio Kawahara , Shinya Goto , Osamu Kurahashi , Tsuyoshi Nakamatsu
IPC分类号： C12P1304
CPC分类号： C12P13/222 , C12P13/04 , C12P13/08 , C12P13/14
摘要： A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a &sgr; factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.
摘要翻译：利用微生物通过在培养基中培养微生物发酵生产有用物质如氨基酸，使发酵产物在培养基中产生和积累，收集发酵产物，其中所用的微生物通过引入进行修饰编码热休克蛋白的基因中的至少一种和编码特异性用于热休克蛋白基因的西格玛因子的基因以增强细胞中的热休克蛋白的表达量，由此允许微生物添加否则会抑制微生物的生长和/或发酵产物的产生。

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