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1. 发明授权

US08524476B2 Bacterium producing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity and a method for manufacturing the product 有权
标题翻译：产生由具有2-氧戊二酸依赖性酶活性的蛋白质催化的反应产物的细菌和制备该产物的方法
公开(公告)号：US08524476B2
公开(公告)日：2013-09-03
申请号：US12819485
申请日：2010-06-21
申请人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko , Tatyana Aleksandrovna Bachina , Yuki Imabayashi , Masakazu Sugiyama , Shunichi Suzuki
发明人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko , Tatyana Aleksandrovna Bachina , Yuki Imabayashi , Masakazu Sugiyama , Shunichi Suzuki
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12P13/06 , C12P1/04 , C07H21/04
CPC分类号： C12P13/06 , C12N9/0069 , C12N9/0071 , C12P13/24
摘要： A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.
摘要翻译：使用由DNA片段转化的细菌制造由具有2-氧戊二酸依赖性酶活性的蛋白质（例如（2S，3R，4S）-4-羟基-L-异亮氨酸或其盐）催化的反应产物的方法含有编码具有2-氧戊二酸依赖性酶活性的蛋白质的基因，例如L-异亮氨酸双加氧酶活性; 并且其中所述细菌具有产生如（2S，3R，4S）-4-羟基-L-异亮氨酸的产物的能力。

2. 发明申请

US20100330622A1 BACTERIUM PRODUCING A PRODUCT OF A REACTION CATALYZED BY A PROTEIN HAVING 2-OXOGLUTARATE-DEPENDENT ENZYME ACTIVITY AND A METHOD FOR MANUFACTURING THE PRODUCT 有权
标题翻译：生产由具有2-羟基脂肪酸依赖性酶活性的蛋白质催化的反应产物的细菌和制造该产品的方法
公开(公告)号：US20100330622A1
公开(公告)日：2010-12-30
申请号：US12819485
申请日：2010-06-21
申请人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko , Tatyana Aleksandrovna Bachina , Yuki Imabayashi , Masakazu Sugiyama , Shunichi Suzuki
发明人： Sergey Vasilievich Smirnov , Natalia Nikolaevna Samsonova , Veronika Aleksandrovna Kotliarova , Natalia Yurievna Rushkevich , Olga Sergeevna Beznoschenko , Tatyana Aleksandrovna Bachina , Yuki Imabayashi , Masakazu Sugiyama , Shunichi Suzuki
IPC分类号： C12P13/24 , C12N1/21 , C12P1/04 , C12P13/06
CPC分类号： C12P13/06 , C12N9/0069 , C12N9/0071 , C12P13/24
摘要： A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.
摘要翻译：使用由DNA片段转化的细菌制造由具有2-氧戊二酸依赖性酶活性的蛋白质（例如（2S，3R，4S）-4-羟基-L-异亮氨酸或其盐）催化的反应产物的方法含有编码具有2-氧戊二酸依赖性酶活性的蛋白质的基因，例如L-异亮氨酸双加氧酶活性; 并且其中所述细菌具有产生如（2S，3R，4S）-4-羟基-L-异亮氨酸的产物的能力。

3. 发明授权

US07858348B2 N-acetyl-(R,S)-beta-amino acid acylase gene 有权
标题翻译： N-乙酰基 - （R，S） - 氨基酸酰基转移酶基因
公开(公告)号：US07858348B2
公开(公告)日：2010-12-28
申请号：US11782260
申请日：2007-07-24
申请人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
发明人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04
CPC分类号： C12N9/80
摘要： The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.
摘要翻译：本发明提供编码N-乙酰基 - （R，S） - 氨基酸酰基转移酶的基因。从细菌细胞中分离纯化N-乙酰基 - （R，S） - 氨基酸酰基转移酶，测定核苷酸序列。宿主如大肠杆菌用于构建这些基因的高表达系统。由伯克霍尔德氏菌属（Burkholderia sp。）生产的N-乙酰基 - （R） - AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。由伯克霍尔德氏菌属（Burkholderia sp。）生产的N-乙酰基 - （S） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 9.由Variovorax sp。生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110348（FERM BP-10367）包括例如由SEQ ID NO：1所示的氨基酸序列组成的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 11。

4. 发明授权

US07851187B2 Method of producing L-serine derivative and enzyme used therefore 有权
标题翻译：因此生产L-丝氨酸衍生物和酶的方法
公开(公告)号：US07851187B2
公开(公告)日：2010-12-14
申请号：US11942873
申请日：2007-11-20
申请人： Shinji Kuroda , Hiroyuki Nozaki , Kunihiko Watanabe , Kenzo Yokozeki , Yuki Imabayashi
发明人： Shinji Kuroda , Hiroyuki Nozaki , Kunihiko Watanabe , Kenzo Yokozeki , Yuki Imabayashi
IPC分类号： C12P13/06 , C12N9/00 , C12N9/10 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/1014 , C12P13/06 , C12Y201/02001
摘要： The present invention provides a new method for producing serine derivatives and their optically-activated derivatives in a convenient manner. In the presence of an enzyme, an L-α-amino acid of formula (I): (in the formula (I), R1 is a hydrocarbon group) is reacted with an aldehyde of formula (II): (in the formula (II), R2 is a hydrocarbon) to produce an L-serine derivative of formula (III). .
摘要翻译：本发明提供了以方便的方式制备丝氨酸衍生物及其光活性衍生物的新方法。在酶的存在下，式（I）:(式（I）中的R 1为烃基）的式（I）的L-α-氨基酸与式（II）的醛反应：（在式 II），R 2为烃），得到式（III）的L-丝氨酸衍生物。。

5. 发明申请

US20080241895A1 N-Acetyl-(R,S)-beta-Amino Acid Acylase Gene 有权
标题翻译： N-乙酰基 - （R，S） - 氨基酸酰基酶
公开(公告)号：US20080241895A1
公开(公告)日：2008-10-02
申请号：US11782260
申请日：2007-07-24
申请人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
发明人： Shunichi Suzuki , Yuki Imabayashi , Kunihiko Watanabe , Hisashi Kawasaki , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C07H21/04 , C12N9/80 , C12N1/21
CPC分类号： C12N9/80
摘要： The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.
摘要翻译：本发明提供编码N-乙酰基 - （R，S） - 氨基酸酰基转移酶的基因。从细菌细胞中分离纯化N-乙酰基 - （R，S） - 氨基酸酰基转移酶，确定核苷酸序列。宿主如大肠杆菌用于构建这些基因的高表达系统。伯克霍尔德氏菌属生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。由伯克霍尔德氏菌属生产的N-乙酰基 - （S） - 氨基酸酰基转移酶 AJ110349（FERM BP-10366）包括例如具有SEQ ID NO：1所示氨基酸序列的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 9.由Variovorax sp。生产的N-乙酰基 - （R） - 氨基酸酰基转移酶 AJ110348（FERM BP-10367）包括例如由SEQ ID NO：1所示的氨基酸序列组成的蛋白质。 ID。没有。编码该酶的基因包括例如具有SEQ ID NO：1所示核苷酸序列的DNA。 ID。没有。 11。

6. 发明申请

US20090170171A1 Method of Producing L-Serine Derivative and Enzyme Used Therefore 有权
标题翻译：因此生产L-丝氨酸衍生物和酶的方法
公开(公告)号：US20090170171A1
公开(公告)日：2009-07-02
申请号：US11942873
申请日：2007-11-20
申请人： Shinji Kuroda , Hiroyuki Nozaki , Kunihiko Watanabe , Kenzo Yokozeki , Yuki Imabayashi
发明人： Shinji Kuroda , Hiroyuki Nozaki , Kunihiko Watanabe , Kenzo Yokozeki , Yuki Imabayashi
IPC分类号： C12P13/06 , C12N9/00 , C07H21/00 , C12N1/00
CPC分类号： C12N9/1014 , C12P13/06 , C12Y201/02001
摘要： The present invention provides a new method for producing serine derivatives and their optically-activated derivatives in a convenient manner. In the presence of an enzyme, an L-α-amino acid of formula (I): (in the formula (I), R1 is a hydrocarbon group) is reacted with an aldehyde of formula (II): (in the formula (II), R2 is a hydrocarbon) to produce an L-serine derivative of formula (III).
摘要翻译：本发明提供了以方便的方式制备丝氨酸衍生物及其光活性衍生物的新方法。在酶的存在下，式（I）:(式（I）中的R 1为烃基）的式（I）的L-α-氨基酸与式（II）的醛反应：（在式 II），R 2为烃），得到式（III）的L-丝氨酸衍生物。

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