会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 29. 发明授权
    • Method of producing L-serine by fermentation
    • 通过发酵生产L-丝氨酸的方法
    • US06258573B1
    • 2001-07-10
    • US09222786
    • 1998-12-30
    • Mikiko SugaMasakazu SugimotoTsuyoshi OsumiTsuyoshi NakamatsuWataru HibinoMika Ito
    • Mikiko SugaMasakazu SugimotoTsuyoshi OsumiTsuyoshi NakamatsuWataru HibinoMika Ito
    • C12P1306
    • C12N9/0006C12P13/06C12R1/13C12R1/15C12R1/19
    • Disclosed is a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity. Also, disclosed are D-3-phosphoglycerate dehydrogenase derived from a coryneform bacterium, in which feedback inhibition by L-serine is desensitized; the D-3-phosphoglycerate dehydrogenase, which is obtainable from a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity; the D-3-phosphoglycerate dehydrogenase having an amino acid sequence depicted in SEQ ID NO: 12 in Sequence Listing or the sequence including substitution, addition or deletion of one or more amino acids, wherein an amino acid residue corresponding to the 325th glutamic acid residue of the amino acid sequence in the SEQ ID NO: 12 is replaced with an amino acid other than glutamic acid; the D-3-phosphoglycerate dehydrogenase that has an amino acid sequence depicted in SEQ ID NO: 11 in Sequence Listing; a DNA coding for the D-3-phosphoglycerate dehydrogenase described above; the DNA that has a base sequence depicted in SEQ ID NO: 13 in Sequence Listing; a coryneform bacterium which harbors a recombinant DNA containing the DNA; and a method of producing L-serine, comprising the steps of cultivating the bacterium described above in a medium to allow accumulation of L-serine in the medium, and collecting the L-serine from the medium.
    • 公开了具有对丝氨酸或β-(2-噻吩基)-DL-丙氨酸具有L-丝氨酸生产力的抗性的棒状细菌。 此外,公开了衍生自棒状细菌的D-3-磷酸甘油酸脱氢酶,其中L-丝氨酸的反馈抑制被脱敏; D-3-磷酸甘油酸脱氢酶,其可从具有对β-丝氨酸或β-(2-噻吩基)-DL-丙氨酸具有抗丝氨酸生产力的棒状细菌获得; 具有序列表中SEQ ID NO:12所示氨基酸序列的D-3-磷酸甘油酸脱氢酶或包含一个或多个氨基酸的取代,添加或缺失的序列,其中对应于第325位谷氨酸残基的氨基酸残基 SEQ ID NO:12中的氨基酸序列被除了谷氨酸以外的氨基酸替换; 具有序列表中SEQ ID NO:11所示的氨基酸序列的D-3-磷酸甘油酸脱氢酶; 编码上述D-3-磷酸甘油酸脱氢酶的DNA; 具有序列表中SEQ ID NO:13所示碱基序列的DNA; 含有含DNA的重组DNA的棒状细菌; 以及生产L-丝氨酸的方法,包括以下步骤:在培养基中培养上述细菌以允许L-丝氨酸积累在培养基中,并从培养基中收集L-丝氨酸。
    • 30. 发明授权
    • Method for producing L-lysine
    • 生产L-赖氨酸的方法
    • US06221636B1
    • 2001-04-24
    • US08985916
    • 1997-12-05
    • Atsushi HayakawaMasakazu SugimotoYasuhiko YoshiharaTsuyoshi Nakamatsu
    • Atsushi HayakawaMasakazu SugimotoYasuhiko YoshiharaTsuyoshi Nakamatsu
    • C12P1308
    • C12N9/1217C12N9/88C12P13/08
    • A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA sequence coding for a diaminopimelate decarboxylase; a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a diaminopimelate decarboxylase; and a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture.
    • 在棒状杆菌细胞的细胞中自主复制的重组DNA,其中包含编码天冬氨酸激酶的DNA序列,其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏,以及编码二氨基庚二酸脱羧酶的DNA序列; 携带天冬氨酸激酶的棒状细菌,其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏,并且包含编码二氨基庚二酸脱羧酶的增强的DNA序列; 以及L-赖氨酸的制造方法,其特征在于,在合适的培养基中培养棒状细菌,使L-赖氨酸在细菌培养物中产生并积累,从培养物中收集L-赖氨酸。