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21. 发明授权

US08183017B2 Method of producing L-lysine 有权
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US08183017B2
公开(公告)日：2012-05-22
申请号：US12915793
申请日：2010-10-29
申请人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C12P21/04
CPC分类号： C12N9/0016 , C12N9/1217 , C12N9/88 , C12N15/52 , C12N15/77 , C12P13/08
摘要： The ability and speed with which a coryneform bacterium can produce L-lysine are improved when the coryneform bacterium contains an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized. This is accomplished by successively enhancing the DNA coding for dihydrodipicolinate reductase, the DNA coding for dihydrodipicolinate synthase, the DNA coding for diaminopimelate decarboxylase, and the DNA coding for diaminopimelate dehydrogenase.
摘要翻译：当棒状细菌含有其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏的天冬氨酸激酶时，棒状细菌可以产生L-赖氨酸的能力和速度得到改善。这是通过连续增强编码二氢吡啶二羧酸还原酶的DNA，编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA来实现的。

22. 发明授权

US07846698B2 Method of producing L-lysine 有权
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US07846698B2
公开(公告)日：2010-12-07
申请号：US10226136
申请日：2002-08-23
申请人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C12P21/04
CPC分类号： C12N9/0016 , C12N9/1217 , C12N9/88 , C12N15/52 , C12N15/77 , C12P13/08
摘要： The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.
摘要翻译：通过连续增强编码二氢吡啶二羧酸还原酶的DNA，在含有赖氨酸和L-苏氨酸的反馈抑制实质上脱敏的天麸蛋白的棒状细菌的L-赖氨酸生产能力和L-赖氨酸生产速度得到改善，编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA，以及编码二氨基庚二酸脱氢酶的DNA。

23. 发明授权

US07833760B2 Process for the production of β-amino acids using acylase 有权
标题翻译：使用酰基转移酶生产β-氨基酸的方法
公开(公告)号：US07833760B2
公开(公告)日：2010-11-16
申请号：US11924760
申请日：2007-10-26
申请人： Tsuyoshi Nakamatsu , Hisashi Kawasaki , Kunihiko Watanabe , Masakazu Nakazawa , Kunisuke Izawa
发明人： Tsuyoshi Nakamatsu , Hisashi Kawasaki , Kunihiko Watanabe , Masakazu Nakazawa , Kunisuke Izawa
IPC分类号： C12P13/04 , C12N1/20
CPC分类号： C12P13/04 , C12P41/006 , C12R1/01
摘要： The present invention relates to a Variovorax sp. which produces an acylase having an asymmetric hydrolysis activity on an N-acetyl β-amino acid to selectively produce an R-β-amino acid, and a Burkholderia sp. which produces both an acylase having an asymmetric hydrolysis activity on an N-acetyl β-amino acid to selectively produce an S-β-amino acid and an acylase having an asymmetric hydrolysis activity of an N-acetyl β-amino acid to selectively produce an R-β-amino acid, and a process for the selective production of an S-, or R-β-amino acid using the above strains.
摘要翻译：本发明涉及一种Variovorax sp。其产生对N-乙酰基和氨基酸具有不对称水解活性的酰基转移酶以选择性地产生R-和β-氨基酸，以及伯克霍尔德氏菌属（Burkholderia sp。其产生对N-乙酰基和氨基酸具有不对称水解活性的酰基转移酶，以选择性地产生具有N-乙酰基 - 氨基酸的不对称水解活性的S-＆bgr。 - 氨基酸和酰基转移酶至选择性地产生R-和β-氨基酸，以及使用上述菌株选择性地制备S-或R-和 - - - - 氨基酸的方法。

24. 发明申请

US20070092953A1 METHOD FOR PRODUCING L-GLUTAMINE BY FERMENTATION AND L-GLUTAMINE PRODUCING BACTERIUM 审中-公开
标题翻译：通过发酵和L-谷氨酰胺生产细菌生产L-谷氨酰胺的方法
公开(公告)号：US20070092953A1
公开(公告)日：2007-04-26
申请号：US11616463
申请日：2006-12-27
申请人： Jun Nakamura , Hiroshi Izui , Kayo Moriguchi , Hiroki Kawashima , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Jun Nakamura , Hiroshi Izui , Kayo Moriguchi , Hiroki Kawashima , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/14 , C12N1/21
CPC分类号： C12N9/0016 , C12N9/1241 , C12N9/93 , C12P13/04 , C12P13/14
摘要： L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.
摘要翻译： L-谷氨酰胺通过培养具有L-谷氨酰胺生产能力并已被修饰以使其细胞内谷氨酰胺合成酶活性得到增强的棒状细菌产生，优选已被进一步修饰以使其细胞内谷氨酸脱氢酶活性应当增强，在培养基中产生和积累L-谷氨酰胺并收集L-谷氨酰胺的培养基。

25. 发明授权

US07125977B2 Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria 有权
标题翻译：来自嗜热棒状杆菌的氨基酸生物合成途径耐热酶基因
公开(公告)号：US07125977B2
公开(公告)日：2006-10-24
申请号：US11073550
申请日：2005-03-08
申请人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
发明人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
IPC分类号： C07H21/04
CPC分类号： C12Y108/01004 , C07K14/34 , C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1205 , C12N9/2408 , C12N9/88 , C12Y101/01041 , C12Y102/01051 , C12Y102/04002 , C12Y104/01004 , C12Y203/03001 , C12Y207/01011 , C12Y302/01026 , C12Y401/01031 , C12Y401/03001 , C12Y402/01003 , C12Y604/01001 , C12Y604/01002
摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.
摘要翻译：基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为与谷氨酸棒杆菌相比在更高的温度下起作用的酶。通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

26. 发明授权

US06995250B1 Thermophilic amino acid biosynthesis system enzyme gene of thermotolerant coryneform bacterium 失效
标题翻译：耐热棒状杆菌型嗜热氨基酸生物合成酶基因
公开(公告)号：US06995250B1
公开(公告)日：2006-02-07
申请号：US10089057
申请日：2000-10-04
申请人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
发明人： Seiko Hirano , Eiichiro Kimura , Tsuyoshi Osumi , Kazuhiko Matsui , Yoshio Kawahara , Gen Nonaka , Yumi Matsuzaki , Naoki Akiyoshi , Kanae Nakamura , Osamu Kurahashi , Tsuyoshi Nakamatsu , Shinichi Sugimoto
IPC分类号： C07H21/02 , C07H21/04 , C12P21/06
CPC分类号： C12Y108/01004 , C07K14/34 , C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1205 , C12N9/2408 , C12N9/88 , C12Y101/01041 , C12Y102/01051 , C12Y102/04002 , C12Y104/01004 , C12Y203/03001 , C12Y207/01011 , C12Y302/01026 , C12Y401/01031 , C12Y401/03001 , C12Y402/01003 , C12Y604/01001 , C12Y604/01002
摘要： A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.
摘要翻译：基于对应于编码源自热棒状棒杆菌的L-氨基酸生物合成途径的酶的基因的已知基因序列的各种微生物，观察到在氨基酸水平上保持的区域的多个引物组，优选为与谷氨酸棒杆菌相比在更高的温度下起作用的酶。通过使用热源性棒状杆菌的引物和染色体DNA作为模板进行PCR。使用已经获得扩增片段的引物作为引物用于筛选，从嗜热氨基酸棒杆菌的染色体DNA的质粒文库中选择含有靶DNA片段的克隆。

27. 发明申请

US20060003424A1 Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
标题翻译：构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法
公开(公告)号：US20060003424A1
公开(公告)日：2006-01-05
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/22 , C12P13/14 , C12P13/08 , C12N1/21
CPC分类号： C12P13/14 , C12N15/67
摘要： A method of producing coryneform bacteria having an improved amino acid- or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid d- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method can construct a mutant capable of suitably enriching or controling the expression of an intended gene without using a plasmid and also capable of producing amino acids in a high yield, by the recombination or mutation.
摘要翻译：具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌的染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使其接近共有序列，获得棒状杆菌氨基酸d- 或产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。该方法可以构建能够适当地富集或控制预期基因的表达而不使用质粒并且还能够通过重组或突变以高产率产生氨基酸的突变体。

28. 发明授权

US06962805B2 Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权
标题翻译：构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法
公开(公告)号：US06962805B2
公开(公告)日：2005-11-08
申请号：US09577005
申请日：2000-05-25
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12N15/37 , C12N1/21 , C12N15/67 , C12P13/04 , C12P13/14 , C07H21/04 , C12N1/20 , C12N15/74 , C12Q1/32
CPC分类号： C12P13/14 , C12N15/67
摘要： A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.
摘要翻译：制备具有改善的氨基酸或核酸生产力的棒状细菌的方法包括以下步骤：在棒状细菌染色体上的氨基酸或核酸 - 生物合成基因的启动子序列中引入突变，使其接近共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化使其接近共有序列，以获得棒状细胞氨基酸或核酸的突变体培养微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。该方法允许构建能够富集或控制预期基因的表达而不使用质粒的突变体，并通过重组或突变促进高产量的氨基酸的产生。

29. 发明授权

US06258573B1 Method of producing L-serine by fermentation 失效
标题翻译：通过发酵生产L-丝氨酸的方法
公开(公告)号：US06258573B1
公开(公告)日：2001-07-10
申请号：US09222786
申请日：1998-12-30
申请人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
发明人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
IPC分类号： C12P1306
CPC分类号： C12N9/0006 , C12P13/06 , C12R1/13 , C12R1/15 , C12R1/19
摘要： Disclosed is a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity. Also, disclosed are D-3-phosphoglycerate dehydrogenase derived from a coryneform bacterium, in which feedback inhibition by L-serine is desensitized; the D-3-phosphoglycerate dehydrogenase, which is obtainable from a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity; the D-3-phosphoglycerate dehydrogenase having an amino acid sequence depicted in SEQ ID NO: 12 in Sequence Listing or the sequence including substitution, addition or deletion of one or more amino acids, wherein an amino acid residue corresponding to the 325th glutamic acid residue of the amino acid sequence in the SEQ ID NO: 12 is replaced with an amino acid other than glutamic acid; the D-3-phosphoglycerate dehydrogenase that has an amino acid sequence depicted in SEQ ID NO: 11 in Sequence Listing; a DNA coding for the D-3-phosphoglycerate dehydrogenase described above; the DNA that has a base sequence depicted in SEQ ID NO: 13 in Sequence Listing; a coryneform bacterium which harbors a recombinant DNA containing the DNA; and a method of producing L-serine, comprising the steps of cultivating the bacterium described above in a medium to allow accumulation of L-serine in the medium, and collecting the L-serine from the medium.
摘要翻译：公开了具有对丝氨酸或β-（2-噻吩基）-DL-丙氨酸具有L-丝氨酸生产力的抗性的棒状细菌。此外，公开了衍生自棒状细菌的D-3-磷酸甘油酸脱氢酶，其中L-丝氨酸的反馈抑制被脱敏; D-3-磷酸甘油酸脱氢酶，其可从具有对β-丝氨酸或β-（2-噻吩基）-DL-丙氨酸具有抗丝氨酸生产力的棒状细菌获得; 具有序列表中SEQ ID NO：12所示氨基酸序列的D-3-磷酸甘油酸脱氢酶或包含一个或多个氨基酸的取代，添加或缺失的序列，其中对应于第325位谷氨酸残基的氨基酸残基 SEQ ID NO：12中的氨基酸序列被除了谷氨酸以外的氨基酸替换; 具有序列表中SEQ ID NO：11所示的氨基酸序列的D-3-磷酸甘油酸脱氢酶; 编码上述D-3-磷酸甘油酸脱氢酶的DNA; 具有序列表中SEQ ID NO：13所示碱基序列的DNA; 含有含DNA的重组DNA的棒状细菌; 以及生产L-丝氨酸的方法，包括以下步骤：在培养基中培养上述细菌以允许L-丝氨酸积累在培养基中，并从培养基中收集L-丝氨酸。

30. 发明授权

US06221636B1 Method for producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US06221636B1
公开(公告)日：2001-04-24
申请号：US08985916
申请日：1997-12-05
申请人： Atsushi Hayakawa , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Atsushi Hayakawa , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P1308
CPC分类号： C12N9/1217 , C12N9/88 , C12P13/08
摘要： A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA sequence coding for a diaminopimelate decarboxylase; a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a diaminopimelate decarboxylase; and a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture.
摘要翻译：在棒状杆菌细胞的细胞中自主复制的重组DNA，其中包含编码天冬氨酸激酶的DNA序列，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，以及编码二氨基庚二酸脱羧酶的DNA序列; 携带天冬氨酸激酶的棒状细菌，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，并且包含编码二氨基庚二酸脱羧酶的增强的DNA序列; 以及L-赖氨酸的制造方法，其特征在于，在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，从培养物中收集L-赖氨酸。

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