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1. 发明授权

US08183017B2 Method of producing L-lysine 有权
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US08183017B2
公开(公告)日：2012-05-22
申请号：US12915793
申请日：2010-10-29
申请人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C12P21/04
CPC分类号： C12N9/0016 , C12N9/1217 , C12N9/88 , C12N15/52 , C12N15/77 , C12P13/08
摘要： The ability and speed with which a coryneform bacterium can produce L-lysine are improved when the coryneform bacterium contains an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized. This is accomplished by successively enhancing the DNA coding for dihydrodipicolinate reductase, the DNA coding for dihydrodipicolinate synthase, the DNA coding for diaminopimelate decarboxylase, and the DNA coding for diaminopimelate dehydrogenase.
摘要翻译：当棒状细菌含有其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏的天冬氨酸激酶时，棒状细菌可以产生L-赖氨酸的能力和速度得到改善。这是通过连续增强编码二氢吡啶二羧酸还原酶的DNA，编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA来实现的。

2. 发明授权

US07846698B2 Method of producing L-lysine 有权
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US07846698B2
公开(公告)日：2010-12-07
申请号：US10226136
申请日：2002-08-23
申请人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Seiko Otsuna , Masakazu Sugimoto , Masako Izui , Atsushi Hayakawa , Eiichi Nakano , Masaki Kobayashi , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P13/04 , C12P21/04
CPC分类号： C12N9/0016 , C12N9/1217 , C12N9/88 , C12N15/52 , C12N15/77 , C12P13/08
摘要： The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.
摘要翻译：通过连续增强编码二氢吡啶二羧酸还原酶的DNA，在含有赖氨酸和L-苏氨酸的反馈抑制实质上脱敏的天麸蛋白的棒状细菌的L-赖氨酸生产能力和L-赖氨酸生产速度得到改善，编码二氢吡啶二羧酸合成酶的DNA，编码二氨基庚二酸脱羧酶的DNA，以及编码二氨基庚二酸脱氢酶的DNA。

3. 发明授权

US6090597A Method of producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US6090597A
公开(公告)日：2000-07-18
申请号：US852730
申请日：1997-05-07
申请人： Seiko Hirano , Masakazu Sugimoto , Eiichi Nakano , Masako Izui , Atsushi Hayakawa , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Seiko Hirano , Masakazu Sugimoto , Eiichi Nakano , Masako Izui , Atsushi Hayakawa , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/06 , C12N9/88 , C12N15/77 , C12P13/08 , C12R1/13 , C12N1/20 , C12N15/00
CPC分类号： C12N9/0016 , C12N15/77 , C12N9/88 , C12P13/08
摘要： A coryneform bacterium in which a DNA coding for a diaminopimelate decarboxylase and a DNA coding for a diaminopimelate dehydrogenase are enhanced is cultivated in a medium to allow L-lysine to be produced and accumulated in a culture, and L-lysine is collected from the culture.
摘要翻译：在培养基中培养其中编码二氨基庚二酸脱羧酶的DNA和编码二氨基庚二酸脱氢酶的DNA的棒状细菌在培养基中培养以使L-赖氨酸在培养物中产生并积累，从培养物中收集L-赖氨酸。

4. 发明授权

US06221636B1 Method for producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US06221636B1
公开(公告)日：2001-04-24
申请号：US08985916
申请日：1997-12-05
申请人： Atsushi Hayakawa , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Atsushi Hayakawa , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12P1308
CPC分类号： C12N9/1217 , C12N9/88 , C12P13/08
摘要： A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA sequence coding for a diaminopimelate decarboxylase; a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a diaminopimelate decarboxylase; and a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture.
摘要翻译：在棒状杆菌细胞的细胞中自主复制的重组DNA，其中包含编码天冬氨酸激酶的DNA序列，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，以及编码二氨基庚二酸脱羧酶的DNA序列; 携带天冬氨酸激酶的棒状细菌，其中L-赖氨酸和L-苏氨酸的反馈抑制基本上脱敏，并且包含编码二氨基庚二酸脱羧酶的增强的DNA序列; 以及L-赖氨酸的制造方法，其特征在于，在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，从培养物中收集L-赖氨酸。

5. 发明授权

US6004773A Method for producing L-lysine 失效
标题翻译：生产L-赖氨酸的方法
公开(公告)号：US6004773A
公开(公告)日：1999-12-21
申请号：US985908
申请日：1997-12-05
申请人： Masayuki Araki , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Masayuki Araki , Masakazu Sugimoto , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N1/21 , C12N9/02 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/53 , C12N15/54 , C12N15/60 , C12N15/77 , C12P13/08 , C12P1/00 , C07H21/04 , C12N15/63 , C12P21/02
CPC分类号： C12N9/001 , C12N15/77 , C12N9/1096 , C12N9/1217 , C12N9/88 , C12P13/08
摘要： A coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a dihydrodipicolinate reductase, an enhanced DNA sequence coding for dihydropicolinate reductase, an enhance DNA sequence coding for dihydropicolinate synthase, an enhanced DNA sequence coding for diaminopimelate decarboxylase and an enhanced DNA sequence coding for aspartate aminotransferase; a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture; and a recombinant DNA usable for production of the coryneform bacterium.
摘要翻译：含有赖氨酸和L-苏氨酸的反馈抑制基本脱敏的天冬氨酸激酶的棒状细菌，包含编码二氢吡啶二羧酸还原酶的增强型DNA序列，编码二氢吡啶啉酮还原酶的增强型DNA序列，编码二氢吡啶酸盐的增强型DNA序列合成酶，编码二氨基庚二酸脱羧酶的增强的DNA序列和编码天冬氨酸转氨酶的增强的DNA序列; 一种生产L-赖氨酸的方法，包括以下步骤：在合适的培养基中培养棒状细菌，使L-赖氨酸在细菌培养物中产生并积累，并从培养物中收集L-赖氨酸; 以及可用于生产棒状细菌的重组DNA。

6. 发明授权

US5929221A Gene derived from coryneform bacteria and use thereof 失效
标题翻译：衍生自棒状细菌的基因及其用途
公开(公告)号：US5929221A
公开(公告)日：1999-07-27
申请号：US693228
申请日：1996-08-22
申请人： Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N15/09 , C07K14/34 , C12N1/21 , C12N15/31 , C12P13/08 , C12P13/14 , C12R1/15 , C07H21/02 , C07H21/04 , C12N15/00 , C12Q1/68
CPC分类号： C12P13/14 , C07K14/34 , C12P13/08
摘要： The L-lysine productivity of an L-lysine producing Coryneform bacterium is enhanced by amplifying a novel gene derived from a Coryneform bacterium and participating in L-glutamic acid production, while the L-glutamic acid productivity of an L-glutamic acid-producing Coryneform bacterium is enhanced by suppressing the function of the gene.
摘要翻译： PCT No.PCT / JP95 / 00269 Sec。 371日期：1996年8月22日 102（e）日期1996年8月22日PCT提交1995年2月23日PCT公布。 WO95 / 23224 PCT公开号日期1995年8月31日L-赖氨酸生产棒状杆菌细菌的L-赖氨酸生产力通过扩增衍生自棒状杆菌属细菌的新基因并参与L-谷氨酸生产而增强，而L-谷氨酸生产力为L- 通过抑制基因的功能来增强产生谷氨酸的棒状细菌。

7. 发明授权

US5846790A Methods of producing L-lysine and L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-赖氨酸和L-谷氨酸的方法
公开(公告)号：US5846790A
公开(公告)日：1998-12-08
申请号：US776597
申请日：1997-02-18
申请人： Eiichiro Kimura , Yoko Asakura , Akinori Uehara , Sumio Inoue , Yoshio Kawahara , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
发明人： Eiichiro Kimura , Yoko Asakura , Akinori Uehara , Sumio Inoue , Yoshio Kawahara , Yasuhiko Yoshihara , Tsuyoshi Nakamatsu
IPC分类号： C12N1/21 , C12N15/09 , C12P13/08 , C12P13/14
CPC分类号： C12P13/14 , C12P13/08 , Y10S435/84 , Y10S435/843
摘要： A mutant strain having an ability to produce L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to produce and accumulate L-glutamic acid in the medium. A mutant strain having an ability to produce L-lysine and L-glutamic acid in the absence of any biotin action-suppressing agent in a medium containing an excessive amount of biotin is obtained by giving temperature sensitivity with respect to a biotin action-suppressing agent and giving L-lysine productivity to a coryneform L-glutamic acid-producing bacterium. This strain is cultivated in a liquid medium to simultaneously produce and accumulate L-lysine and L-glutamic acid in the medium.
摘要翻译： PCT No.PCT / JP95 / 01586 Sec。 371日期1997年2月18日 102（e）1997年2月18日PCT PCT 1995年8月9日PCT公布。出版物WO96 / 06180 日期：1996年2月29日在具有生物素活性抑制剂的情况下，在含有过量生物素的培养基中，不存在生成L-谷氨酸的能力的突变株是通过赋予生物素作用抑制代谢产L-谷氨酸棒状细菌。将该菌株在液体培养基中培养以在培养基中产生并积累L-谷氨酸。通过赋予生物素活性抑制剂的温度敏感度，获得在含有过量生物素的培养基中不存在任何生物素活性抑制剂时能够产生L-赖氨酸和L-谷氨酸的突变株并向L-谷氨酸棒状杆菌产生细菌提供L-赖氨酸生产力。该菌株在液体培养基中培养，以在培养基中同时产生和积累L-赖氨酸和L-谷氨酸。

8. 发明授权

US06852516B2 Method for producing L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-谷氨酸的方法
公开(公告)号：US06852516B2
公开(公告)日：2005-02-08
申请号：US10307320
申请日：2002-12-02
申请人： Wataru Hibino , Yasuhiko Yoshihara , Masakazu Sugimoto , Harufumi Miwa
发明人： Wataru Hibino , Yasuhiko Yoshihara , Masakazu Sugimoto , Harufumi Miwa
IPC分类号： C12N15/09 , C07C51/00 , C12N1/21 , C12N15/00 , C12P13/14 , C12R1/13 , C12R1/15 , C07H21/04 , C12N1/20 , C12N9/00
CPC分类号： C12P13/14
摘要： A coryneform bacterium which has enhanced intracellular pyruvate carboxylase activity obtained by increasing copy number of a gene encoding the intracellular pyruvate carboxylase, or by enhancing function of a expression regulatory sequence for the gene, and has L-glutamic acid-producing ability is cultured in a medium so that L-glutamic acid should be produced and accumulated in the culture, and L-glutamic acid is collected from the culture.
摘要翻译：通过增加编码细胞内丙酮酸羧化酶的基因的拷贝数或通过增强基因的表达调节序列的功能而获得的具有增强的细胞内丙酮酸羧化酶活性并具有L-谷氨酸生产能力的棒状细菌被培养在培养基中生成L-谷氨酸并在培养物中积累，从培养物中收集L-谷氨酸。

9. 发明授权

US5179010A Fermentation process for producing L-lysine 失效
标题翻译：发酵L-赖氨酸的发酵工艺
公开(公告)号：US5179010A
公开(公告)日：1993-01-12
申请号：US469687
申请日：1990-01-26
申请人： Yasuhiko Yoshihara , Yoshio Kawahara , Shigeho Ikeda
发明人： Yasuhiko Yoshihara , Yoshio Kawahara , Shigeho Ikeda
IPC分类号： C12N9/02 , C12P13/08
CPC分类号： C12N9/0089 , C12P13/08 , Y10S435/84 , Y10S435/843
摘要： A fermentative process for producing L-lysine is disclosed. The process is based on growing in a culture medium a mutant strain of the genus Brevibacterium or Corynebacterium (1) capable of producing L-lysine, and (2) having an intensified superoxide dismutase activity.
摘要翻译：公开了生产L-赖氨酸的发酵方法。该方法基于在培养基中生长能够产生L-赖氨酸的短杆菌属或棒状杆菌属（1）的突变菌株，和（2）具有增强的超氧化物歧化酶活性。

10. 发明授权

US5196326A Method for concurrent fermentation of basic amino acid and acidic amino acid 失效
标题翻译：碱性氨基酸和酸性氨基酸同时发酵的方法
公开(公告)号：US5196326A
公开(公告)日：1993-03-23
申请号：US655993
申请日：1991-02-15
申请人： Hideo Kuronuma , Harufumi Miwa , Shigeru Nakamori , Toshimasa Ishii , Yasuhiko Yoshihara
发明人： Hideo Kuronuma , Harufumi Miwa , Shigeru Nakamori , Toshimasa Ishii , Yasuhiko Yoshihara
IPC分类号： C12P13/04
CPC分类号： C12P13/04 , Y10S435/84 , Y10S435/843
摘要： A basic L-amino acid and an acidic L-amino acid may be concurrently produced by either culturing a basic L-amino acid-producing bacteria under conditions for producing an acidic L-amino acid or mix-culturing a basic L-amino acid-producing bacteria and an acidic L-amino acid-producing bacteria.
摘要翻译：碱性L-氨基酸和酸性L-氨基酸可以通过在产生酸性L-氨基酸的条件下培养碱性L-氨基酸生产细菌或混合培养碱性L-氨基酸 - 产生细菌和产生酸性L-氨基酸的细菌。

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