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11. 发明授权

US06346615B1 Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US06346615B1
公开(公告)日：2002-02-12
申请号：US09656213
申请日：2000-09-06
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07H1904
CPC分类号： C07D321/00
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T½ kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
摘要翻译：具有烷氧基取代基的双金刚烷基二氧杂环丁烷和二氧杂环丁烷环上苯基或萘基的芳族取代基可以被活化成化学发光，如果芳族取代基具有指定为OX的部分，其中X被允许二氧杂环丁烷的酶切割在与接触。化学发光反应的T 1/2动力学，以及化学发光反应的信号强度或量子产率可以通过选择吸电子基团或给电子基团Z来改变芳香族取代基以外的芳香族取代基的位置邻近二氧杂环丁烷的连接点。信号强度可以通过认可的化学发光增强剂进一步增强。

12. 发明申请

US20070287825A1 Constrained cis-diol-borate bioconjugation system 审中-公开
标题翻译：约束顺式 - 二醇 - 硼酸盐生物共轭体系
公开(公告)号：US20070287825A1
公开(公告)日：2007-12-13
申请号：US11800341
申请日：2007-05-04
申请人： Ricky Baggio , Alison Sparks , Rouh-Rong Juo , Jaime Arenas
发明人： Ricky Baggio , Alison Sparks , Rouh-Rong Juo , Jaime Arenas
IPC分类号： C07C321/12 , C07C33/18 , C07C53/00 , C07D209/02 , C07D317/70 , C07D487/00 , C07H21/00 , C07K16/00 , C07K2/00 , C08B37/00
CPC分类号： B82Y30/00 , A61K47/54 , C07H21/04 , G01N33/54353 , Y10S530/812
摘要： The invention pertains to bioconjugation systems comprising sterically constrained cis-diols and borates.
摘要翻译：本发明涉及包含空间受限的顺式二醇和硼酸盐的生物共轭体系。

13. 发明申请

US20070184491A1 Dendritic chemiluminescent substrates 有权
标题翻译：树枝状化学发光底物
公开(公告)号：US20070184491A1
公开(公告)日：2007-08-09
申请号：US11730715
申请日：2007-04-03
申请人： Alison Sparks
发明人： Alison Sparks
IPC分类号： G01N33/53 , C12Q1/66
CPC分类号： G01N33/533 , G01N33/543 , G01N33/582 , Y10S436/805
摘要： Chemiluminescent substrate delivery systems comprising a conjugate a dendrimer and at least one chemiluminescent substrate are provided. The substrate delivery systems can also include a chemiluminescence enhancer. The dendrimer/chemiluminescent substrate conjugates can be used in kits including an enzyme capable of activating the chemiluminescent substrate to produce a peroxygenated intermediate that decomposes to produce light. The dendrimer/chemiluminescent substrate conjugates can be used in assays to detect the presence of an analyte (e.g., an enzyme, an antibody, an antigen or a nucleic acid) in a sample.
摘要翻译：提供包含树枝状大分子和至少一种化学发光底物的共轭物的化学发光底物递送系统。底物递送系统还可以包括化学发光增强剂。树枝状聚合物/化学发光底物共轭物可以用于包括能够激活化学发光底物以产生分解产生光的过氧化中间体的酶的试剂盒中。树枝状大分子/化学发光底物共轭物可用于检测样品中分析物（例如酶，抗体，抗原或核酸）的存在。

14. 发明申请

US20050277143A1 Constrained cis-diol-borate bioconjugation system 失效
标题翻译：约束顺式 - 二醇 - 硼酸盐生物共轭体系
公开(公告)号：US20050277143A1
公开(公告)日：2005-12-15
申请号：US11126837
申请日：2005-05-11
申请人： Ricky Baggio , Alison Sparks , Rouh-Rong Juo , Jaime Arenas
发明人： Ricky Baggio , Alison Sparks , Rouh-Rong Juo , Jaime Arenas
IPC分类号： A61K47/48 , C07H21/04 , C07K14/47 , C12Q1/68 , G01N33/53 , G01N33/543
CPC分类号： B82Y30/00 , A61K47/54 , C07H21/04 , G01N33/54353 , Y10S530/812
摘要： The invention pertains to bioconjugation systems comprising sterically constrained cis-diols and borates.
摘要翻译：本发明涉及包含空间受限的顺式二醇和硼酸盐的生物共轭体系。

15. 发明申请

US20050026151A1 Simultaneous generation of multiple chemiluminescent signals on solid supports 审中-公开
标题翻译：在固体支持物上同时产生多种化学发光信号
公开(公告)号：US20050026151A1
公开(公告)日：2005-02-03
申请号：US10620333
申请日：2003-07-17
申请人： John Voyta , Robert Smith , Gary Schroth , Alison Sparks , Brooks Edwards
发明人： John Voyta , Robert Smith , Gary Schroth , Alison Sparks , Brooks Edwards
IPC分类号： G01N33/58 , C12Q1/68
CPC分类号： G01N33/581
摘要： A chemiluminescent assay to determine the presence and/or amount of one or more labeled target molecules in a sample is described in which the surface layer of a solid support is contacted with a composition comprising first and second chemiluminescent substrates capable of being activated by first and second enzymes, respectively. A plurality of probes are disposed on the surface layer in discrete areas. At least some of the probes are bound to a first enzyme conjugate comprising the first enzyme and at least some of the probes are bound to a second enzyme conjugate comprising the second enzyme. The resulting chemiluminescent signals are then detected. The method can be used to compare two biological samples (e.g., mRNA populations from different cells) on the same support surface or to provide a chemiluminescent control signal for normalizing chemiluminescent assay data from a biological sample.
摘要翻译：描述了用于确定样品中一种或多种标记的靶分子的存在和/或量的化学发光测定法，其中固体支持物的表面层与包含能够被第一和第二化学发光底物活化的第一和第二化学发光底物的组合物接触，第二种酶。多个探针设置在离散区域的表面层上。至少一些探针与包含第一酶的第一酶结合物结合，并且至少一些探针与包含第二酶的第二酶缀合物结合。然后检测所得到的化学发光信号。该方法可用于比较两个生物样品（例如，来自不同细胞的mRNA群体）在相同的支持物表面上，或提供化学发光控制信号，用于从生物样品中归一化化学发光测定数据。

16. 发明授权

US5869699A 1,2 chemiluminescent dioxetanes of improved performance 失效
公开(公告)号：US5869699A
公开(公告)日：1999-02-09
申请号：US871963
申请日：1997-06-10
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： C07D321/00 , C07F9/655 , C07G3/00 , C12Q1/37 , G01N33/58 , C07F9/28 , C07D305/14
CPC分类号： G01N33/582 , C07D321/00 , C07F9/65512 , C07G3/00 , C12Q1/37 , Y02P20/55
摘要： A new class of stable dioxetanes bears a polycyclic stabilizing group and aryloxy moiety, the oxygen atom of which is provided with a protective group which can be removed by an enzymatic or chemical trigger admixed with the dioxetane. The polycyclic stabilizing group is preferably spiroadamantane. The group further bears an alkoxy, aryloxy, aralkyloxy or cycloalkyloxy moiety which is partially or completely substituted with halogens, particularly fluorine and chlorine. Proper selection of electron active groups on the stabilizing moiety, the aryl group and the OR group yields enhanced enzyme kinetics, superior light intensity and excellent detection sensitivity in various assays.

17. 发明授权

US5851771A Chemiluminescent 1,2-dioxetanes 失效
公开(公告)号：US5851771A
公开(公告)日：1998-12-22
申请号：US588810
申请日：1996-01-19
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： G01N33/532 , A43B3/00 , C07C43/178 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/67 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07C69/00 , C07C69/017 , C07C69/24 , C07C69/78 , C07C69/84 , C07C69/92 , C07C205/06 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C233/75 , C07C271/28 , C07C271/58 , C07C309/75 , C07C311/04 , C07C311/08 , C07C311/16 , C07D321/00 , C07F7/08 , C07F7/18 , C07F9/12 , C07F9/40 , C07F9/655 , C07F9/6574 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/52 , G01N33/535 , G01N33/58
CPC分类号： G01N33/582 , A43B1/0036 , A43B1/0072 , A43B3/0031 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C271/28 , C07C271/58 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/673 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07D321/00 , C07F9/12 , C07F9/4056 , C07F9/65512 , C07F9/65742 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , G01N21/76 , G01N33/581 , C07C2103/74 , C12Q2334/00 , Y10S435/81 , Y10S435/975
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.

18. 发明授权

US5582980A Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US5582980A
公开(公告)日：1996-12-10
申请号：US231673
申请日：1994-04-25
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks
IPC分类号： G01N33/532 , A43B3/00 , C07C43/178 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/67 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07C69/00 , C07C69/017 , C07C69/24 , C07C69/78 , C07C69/84 , C07C69/92 , C07C205/06 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C233/75 , C07C271/28 , C07C271/58 , C07C309/75 , C07C311/04 , C07C311/08 , C07C311/16 , C07D321/00 , C07F7/08 , C07F7/18 , C07F9/12 , C07F9/40 , C07F9/655 , C07F9/6574 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N33/52 , G01N33/535 , G01N33/58 , C12Q1/00 , C07H15/24 , G01N33/53
CPC分类号： G01N33/582 , A43B1/0036 , A43B1/0072 , A43B3/0031 , C07C205/37 , C07C205/44 , C07C217/84 , C07C233/18 , C07C271/28 , C07C271/58 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C43/225 , C07C43/23 , C07C43/315 , C07C45/673 , C07C47/565 , C07C47/575 , C07C65/03 , C07C65/21 , C07D321/00 , C07F9/12 , C07F9/4056 , C07F9/65512 , C07F9/65742 , C07G3/00 , C07H15/203 , C07H15/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , G01N21/76 , G01N33/581 , C07C2103/74 , C12Q2334/00 , Y10S435/81 , Y10S435/975
摘要： Spiroadamantyl dioxetanes bearing an alkoxy substituent, and a phenyl substituted on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a meta-substituted moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity and/or quantum yield of the chemiluminescent reaction, can be altered by addition of a chlorine substituent at position 4 on the phenyl ring. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
摘要翻译：如果芳香族取代基具有指定为OX的间位取代的部分，则可以将带有烷氧基取代基的双金刚烷基二氧杂环丁烷和在二氧杂环丁烷环上取代的苯基活化成化学发光，其中X被允许进行二氧杂环丁烷的酶切割接触。化学发光反应的T + E，fra 1/2 + EE动力学以及化学发光反应的信号强度和/或量子产率可以通过在苯环上的第4位加入氯取代基来改变。信号强度可以通过认可的化学发光增强剂进一步增强。

19. 发明申请

US20050019778A1 Sequential generation of multiple chemiluminescent signals on solid supports 审中-公开
标题翻译：在固体支持物上顺序生成多种化学发光信号
公开(公告)号：US20050019778A1
公开(公告)日：2005-01-27
申请号：US10620332
申请日：2003-07-17
申请人： John Voyta , Robert Smith , Gary Schroth , Alison Sparks , Brooks Edwards , Carolyn Gonzalez
发明人： John Voyta , Robert Smith , Gary Schroth , Alison Sparks , Brooks Edwards , Carolyn Gonzalez
IPC分类号： C12Q20060101 , C12Q1/68 , G01N33/53 , G01N33/535 , G01N33/537 , G01N33/543 , G01N33/545 , G01N33/552 , G01N33/58
CPC分类号： G01N33/543 , C12Q1/6837 , G01N33/58 , C12Q2565/102 , C12Q2563/103 , C12Q2537/149
摘要： Chemiluminescent assays to determine the presence and/or amount of one or more labeled analytes in a sample are described wherein a solid support is contacted sequentially with first and second chemiluminescent substrates each of which are capable of being activated by an enzyme and the resulting chemiluminescent signals are detected. A plurality of probes are disposed on a surface layer of the solid support in a plurality of discrete areas. Some of the probes are bound to a conjugate of the first enzyme and some of the probes are bound to a conjugate of the second enzyme. The assay can be used to compare biological samples (e.g., mRNA populations from different cells) on the same support surface. Alternatively, one of the chemiluminescent signals generated can be used as a control signal for normalizing the chemiluminescent assay data.
摘要翻译：描述了用于确定样品中一种或多种标记的分析物的存在和/或量的化学发光测定法，其中固体支持物依次与第一和第二化学发光底物接触，每个化学发光底物能够被酶活化，并且得到的化学发光信号被检测到。多个探针设置在多个离散区域中的固体支持体的表面层上。一些探针与第一酶的缀合物结合，一些探针与第二酶的缀合物结合。该测定法可用于比较同一支持物表面上的生物学样品（例如来自不同细胞的mRNA群体）。或者，所生成的化学发光信号之一可以用作控制信号，用于归一化化学发光测定数据。

20. 发明授权

US6132956A Chemiluminescent 1,2-dioxetanes 失效
标题翻译：化学发光的1,2-二氧杂环丁烷
公开(公告)号：US6132956A
公开(公告)日：2000-10-17
申请号：US964552
申请日：1997-11-05
申请人： Irena Bronstein , Brooks Edwards , Alison Sparks , John C. Voyta
发明人： Irena Bronstein , Brooks Edwards , Alison Sparks , John C. Voyta
IPC分类号： G01N31/00 , C07D305/06 , C07D321/00 , C07F7/18 , C09K11/07 , C12Q1/34 , G01N21/78 , G01N33/52 , C12Q1/00
CPC分类号： C12Q1/34 , C07D321/00 , C07F7/1856 , G01N33/52 , Y10S435/975
摘要： Dioxetanes that can be triggered by bases are provided which can be used to detect bases in organic solvents, aqueous preparations and detect gaseous bases in the atmosphere. The dioxetanes can be used to detect the release of bases from various labels, as a means to detect the presence of a base released by physical or natural processes, to calibrate light measuring apparatus and to determine the amount of reducing or oxidizing agent present in the base.
摘要翻译：提供可由碱触发的二氧杂环丁烷，可用于检测有机溶剂，含水制剂中的碱，并检测大气中的气态碱。二氧杂环丁烷可用于检测各种标记物中碱的释放，作为检测通过物理或天然过程释放的碱的存在的手段，校准光测量装置并确定存在于基础。

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