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    • 5. 发明申请
    • METHOD AND APPARATUS FOR THE MONITORING OF SAMPLE MILLING IN A CHARGED PARTICLE INSTRUMENT
    • 用于监测充电颗粒仪器中样品铣削的方法和装置
    • WO2011011661A3
    • 2011-04-28
    • PCT/US2010043014
    • 2010-07-23
    • OMNIPROBE INCZAYKOVA-FELDMAN LYUDMILAMOORE THOMASAMADOR GONZALO
    • ZAYKOVA-FELDMAN LYUDMILAMOORE THOMASAMADOR GONZALO
    • H01J37/26H01J37/20H01J37/244
    • H01J37/3056G01N1/32H01J37/304H01J2237/20207H01J2237/2482H01J2237/30466H01J2237/31745
    • An apparatus for monitoring sample milling in a charged-particle instrument has a variable-tilt specimen holder (130) attached to the instrument tilt stage (120). The variable-tilt specimen holder (130) includes a first pivoting plate (260) having a slot (280) for holding a specimen (290) rotatably supported in the variable-tilt specimen holder (130). The first pivoting plate (260) has a range of rotation sufficient to move the preferred axis of thinning of the specimen (290) from a first position where the tilt stage (120) is placed at its maximum range of tilt and the angle between the preferred axis of thinning of the specimen (290) and the axis of the ion beam column (110) of the instrument is greater than zero, to a second position where the preferred axis for thinning of the specimen (290) is substantially parallel to the axis of the ion-beam column (110). A light detector (250) is positioned to intercept light passing through the specimen (290) as it is thinned by ion-beam milling. The intensity of the light passing through the specimen (290) may be compared to the intensity recorded for previous stages of milling to determine an endpoint for milling
    • 用于监测带电粒子仪器中的样品研磨的装置具有附接到仪器倾斜台(120)的可变倾斜试样保持器(130)。 可变倾斜试样架(130)包括第一枢转板(260),其具有用于保持可旋转地支撑在可变倾斜试样保持器(130)中的试样(290)的狭槽(280)。 第一枢转板(260)具有足够的旋转范围,以便将倾斜台(120)放置在其最大倾斜范围的第一位置移动样品(290)的优选稀疏轴线, 样品(290)的薄化的优选轴线和仪器的离子束柱(110)的轴线大于零,到第二位置,其中样品(290)的薄化优选轴线基本上平行于 离子束柱(110)的轴线。 光检测器(250)被定位成通过离子束铣削来遮蔽通过样品(290)的光线。 可以将通过样品(290)的光的强度与先前研磨阶段记录的强度进行比较,以确定铣削的终点
    • 7. 发明专利
    • DE19932270A1
    • 2001-01-11
    • DE19932270
    • 1999-07-05
    • UNIV SCHILLER JENAMOORE THOMAS
    • HORN ANTONKREUSCH STEFANMOORE THOMAS
    • G01N33/68G01N33/48
    • The invention relates to a method for the multi-dimensional analysis of a proteome. The method is used in the biochemical, biotechnological and medical fields and in the pharmaceutical industry for diagnostic purposes and for developing biologically active substances. The aim of the invention is to improve, facilitate and for certain proteins first of all to enable the quantification and identification of the proteins of a proteome. According to the invention, the proteins of a proteome are subjected to a large number n of different separation processes under standardised conditions in such a way, that each respective liquid fraction m1, obtained in a separation stage, delivers m2 liquid fractions in a separation stage which immediately follows. After n separation stages, m1* m2* ....mn = M liquid fractions have been produced which are identified qualitatively or quantitatively by known identification methods using o different analysis methods and which are quantitatively determined also by known quantification methods in such a way, that once the analysis data has been unified in a database, an n-dimensional image of the proteome is obtained which is characterised by identifiers and quantifiers and by the position in the n-dimensional network.