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1. 发明申请

WO2017064371A1 MUCOSA ANALYSIS 审中-公开
标题翻译：粘膜分析
公开(公告)号：WO2017064371A1
公开(公告)日：2017-04-20
申请号：PCT/FI2016/050721
申请日：2016-10-16
申请人： AQSENS OY
发明人： SIIVONEN, Joonas , TIITTANEN, Satu
IPC分类号： G01N33/574
CPC分类号： G01N33/57407 , G01N33/52 , G01N2458/40 , G01N2800/7028
摘要： The present invention is related to a method for determining mucosa alterations in a subject, in particular to a method for determining mucosa alterations in a sample using a reagent comprising a lanthanide(III) ion, and an array of indicator molecules configured to interact with the sample. The reagent comprising the lanthanide(III) ion does not have any sample or indicator molecule specific recognition elements. The invention relates also to an array for determining mucosa alterations, the array comprising one or more indicator molecules configured to interact specifically with the sample and a reagent comprising a lanthanide(III) ion. The reagent comprising a lanthanide(III)does not have any sample or indicator molecule specific recognition elements.
摘要翻译：本发明涉及用于确定受试者中粘膜改变的方法，具体涉及使用包含镧系元素（III）离子的试剂确定样品中的粘膜改变的方法，以及阵列的指示剂分子被配置成与样品相互作用。包含镧系元素（III）离子的试剂不具有任何样品或指示剂分子特异性识别元件。本发明还涉及用于确定粘膜改变的阵列，所述阵列包含一种或多种指示剂分子，所述指示剂分子被配置成与所述样品特异性相互作用，所述试剂包含镧系元素（III）离子。包含镧系元素（III）的试剂不具有任何样品或指示剂分子特异性识别元件。

2. 发明申请

WO2014116631A1 COMPOSITIONS AND METHODS FOR UPCONVERTING LUMINESCENCE WITH ENGINEERED EXCITATION AND APPLICATIONS THEREOF 审中-公开
标题翻译：用于工程激发的中断发光的组合物和方法及其应用
公开(公告)号：WO2014116631A1
公开(公告)日：2014-07-31
申请号：PCT/US2014/012437
申请日：2014-01-22
申请人： UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL
发明人： HAN, Gang , SHEN, Jie
IPC分类号： C09K11/08
CPC分类号： G01N21/6428 , C09K11/02 , C09K11/025 , C09K11/7773 , G01N21/6486 , G01N2021/6439 , G01N2458/30 , G01N2458/40 , Y10T436/143333
摘要： The invention generally relates to materials and methods for creating and/or utilizing upconverting luminescence. More particularly, the invention relates to novel compositions ( e.g. , nanoparticles) and related methods of preparation and use that enable upconverting luminescence with an efficient excitation optimized at about 800 nm. A unique class of cascade sensitized tri-doped UCNPs with a biocompatiable 800 nm excitable property are disclosed herein, for example, tri-doped β-NaYF 4 :Nd,Yb,Er(Tm)/NaYF 4 UCNPs, which employ Nd 3+ as 800 nm photon sensitizer and Yb 3+ as bridging ions, having strong green or blue upconversion emissions without photobleaching.
摘要翻译：本发明一般涉及用于产生和/或利用上转换发光的材料和方法。更具体地说，本发明涉及新颖的组合物（例如纳米颗粒）和相关的制备和使用方法，其能够通过在约800nm处优化的有效激发来上变频发光。本文公开了具有生物相容性800nm可激发特性的独特类级联致敏三掺杂UCNP，例如三掺杂的N a YF 4：Nd，Yb，Er（Tm）/ NaYF 4 UCNP，其采用Nd3 +为800nm 光子增感剂和Yb3 +作为桥接离子，具有强烈的绿色或蓝色上转换排放，无需光漂白。

3. 发明申请

WO2010051544A3 RAPID HOMOGENEOUS DIAGNOSTIC TESTING PLATFORM BASED ON LANTHANIDE FLUORESCENT RESONANCE ENERGY TRANSFER 审中-公开
标题翻译：基于兰萤荧光共振能量传递的快速均匀诊断测试平台
公开(公告)号：WO2010051544A3
公开(公告)日：2010-08-05
申请号：PCT/US2009063002
申请日：2009-11-02
申请人： LUMIPHORE INC , BUTLIN NATHANIEL G , MIMMS LARRY
发明人： BUTLIN NATHANIEL G , MIMMS LARRY
IPC分类号： A61K31/395 , A61K49/14 , A61K49/16 , G01N33/52 , G01N33/563
CPC分类号： G01N33/542 , G01N2458/40
摘要： The present invention provides compositions and methods for detecting the presence of an analyte in a sample. Exemplary compositions comprise macrocyclic lanthanide complexes that are useful for transferring energy to an energy transfer acceptor to indicate the presence of the analyte. The compositions and methods are particularly suited for detecting antibodies in a sample.
摘要翻译：本发明提供了用于检测样品中分析物的存在的组合物和方法。示例性组合物包含大环镧系元素络合物，其可用于将能量转移至能量转移受体以指示分析物的存在。该组合物和方法特别适用于检测样品中的抗体。

4. 发明申请

WO2010012962A2 METHOD FOR DETECTING MEMBRANE PROTEIN INTERNALIZATION 审中-公开
标题翻译：检测膜蛋白内含物的方法
公开(公告)号：WO2010012962A2
公开(公告)日：2010-02-04
申请号：PCT/FR2009051538
申请日：2009-07-30
申请人： CIS BIO INT , ZWIER JURRIAAN , POOLE ROBERT , ANSANAY HERVE , FINK MICHEL , TRINQUET ERIC
发明人： ZWIER JURRIAAN , POOLE ROBERT , ANSANAY HERVE , FINK MICHEL , TRINQUET ERIC
IPC分类号： G01N33/566 , G01N33/58
CPC分类号： G01N33/542 , G01N33/5035 , G01N33/58 , G01N2458/40 , Y10T436/13
摘要： A method for detecting the internalization of a transmembrane protein of interest expressed at the surface of a cell, comprising the following steps: a) labelling the protein of interest with a fluorescent metal complex, the lifetime of which is greater than 0.1 ms; b) adding to the reaction medium a composition capable of causing the internalization of the protein of interest; c) adding to the reaction medium a modulating agent selected from: a. a fluorescent or nonfluorescent FRET acceptor compound compatible with said fluorescent metal complex, the final concentration of which in the reaction medium is greater than 10-7 M; b. a reducing agent, the redox potential of which is less than +0.1 V and preferably between 0.25 and 0.75 V; c. an agent which binds specifically, by noncovalent bonding, with the fluorescent metal complex; d. a metal ion which competes with the rare earth so as to form a nonfluorescent metal complex; d) measuring the luminescence emitted by the reaction medium at the emission wavelength of the fluorescent metal complex and/or at the emission wavelength of the modulating compound when said compound is a fluorescent acceptor compound; e) comparing the signal measured in step d) with a reference signal measured on cells having been subjected only to steps a) and c). Use: Method for detecting membrane protein internalization.
摘要翻译：一种用于检测在细胞表面表达的目标跨膜蛋白的内在化的方法，包括以下步骤：a）用荧光金属络合物标记感兴趣的蛋白质，其寿命大于0.1ms; b）向反应介质中加入能够引起目的蛋白质内化的组合物; c）向反应介质中加入选自以下的调节剂：a。与所述荧光金属络合物相容的荧光或非荧光FRET受体化合物，其最终浓度在反应介质中大于10-7M; 湾还原剂，其氧化还原电位小于+0.1V，优选0.25至0.75V; C。通过非共价键合与荧光金属络合物特异性结合的试剂; 天。与稀土竞争以形成非荧光金属络合物的金属离子; d）当所述化合物是荧光受体化合物时，测量在荧光金属络合物的发射波长处和/或调节化合物的发射波长处的反应介质发射的发光; e）将步骤d）中测量的信号与在仅经受步骤a）和c）的细胞上测量的参考信号进行比较。用途：检测膜蛋白内化的方法。

5. 发明申请

WO2008063721A3 LUMINESCENT MACROCYCLIC LANTHANIDE COMPLEXES 审中-公开
公开(公告)号：WO2008063721A3
公开(公告)日：2008-11-06
申请号：PCT/US2007076047
申请日：2007-08-15
申请人： UNIV CALIFORNIA , RAYMOND KENNETH N , CORNEILLIE TODD M , XU JIDE
发明人： RAYMOND KENNETH N , CORNEILLIE TODD M , XU JIDE
IPC分类号： C07D245/04
CPC分类号： G01N33/582 , G01N2458/40
摘要： The present invention provides a novel class of macrocyclic compounds as well as complexes formed between a metal (e.g., lanthanide) ion and the compounds of the invention. Preferred complexes exhibit high stability as well as high quantum yields of lanthanide ion luminescence in aqueous media without the need for secondary activating agents. Preferred compounds incorporate hydroxy-isophthalamide moieties within their macrocyclic structure and are characterized by surprisingly low, non-specific binding to a variety of polypeptides such as antibodies and proteins as well as high kinetic stability. These characteristics distinguish them from known, open-structured ligands.
摘要翻译：本发明提供了一类新的大环化合物以及在金属（例如镧系元素）离子和本发明的化合物之间形成的络合物。优选的配合物在水性介质中表现出高稳定性以及镧系元素离子发光的高量子产率，而不需要第二活化剂。优选的化合物在其大环结构内掺入羟基间苯二酰胺部分，其特征在于与各种多肽例如抗体和蛋白质以及高动力学稳定性令人惊奇的低非特异性结合。这些特征将它们与已知的开放结构的配体区分开来。

6. 发明申请

WO2006039505A2 LUMINESCENT LANTHANIDE COMPLEXES 审中-公开
标题翻译： LUMINESCENT LANTHANIDE复合物
公开(公告)号：WO2006039505A2
公开(公告)日：2006-04-13
申请号：PCT/US2005035215
申请日：2005-09-30
申请人： MOLECULAR DEVICES CORP
发明人： PARKER DAVID , ATKINSON PAUL , KIELAR FILIP , BOGE ANNEGRET , SPORTSMAN RICHARD J , GAUDET ELIZABETH , YI GEORGE G
IPC分类号： G01N33/53
CPC分类号： C07D471/04 , C07D403/02 , C07D471/14 , C07D491/04 , C07D495/04 , C09B57/10 , G01N33/582 , G01N2458/40 , Y10T436/13
摘要： Systems, including compositions, kits, and methods, particularly for photoluminescence applications. The systems may include, among others, (1) organic chelators, (2) complexes between the chelators and lanthanide ions, and (3) precursors, derivatives, and uses thereof. The chelators may include a 1,4,7,10-tetraazacyclododecane ring system, for example, having the formula:(I) Here, R
摘要翻译：系统，包括组合物，试剂盒和方法，特别是用于光致发光应用。该系统可以包括（1）有机螯合剂，（2）螯合剂和镧系离子之间的络合物，和（3）前体，衍生物及其用途。螯合剂可以包括例如具有下式的1,4,7,10-四氮杂环十二烷环体系：（I）这里，R

7. 发明申请

WO2004097372A2 ELEMENT-CODED AFFINITY TAGS 审中-公开
标题翻译：元素编码的标签
公开(公告)号：WO2004097372A2
公开(公告)日：2004-11-11
申请号：PCT/US2004013248
申请日：2004-04-28
申请人： UNIV CALIFORNIA , MEARES CLAUDE F , WHETSTONE PAUL A , CORNEILLIE TODD M , BUTLIN NATHANIEL G
发明人： MEARES CLAUDE F , WHETSTONE PAUL A , CORNEILLIE TODD M , BUTLIN NATHANIEL G
IPC分类号： C12Q1/68 , G01N33/48 , G01N33/50 , G01N33/543 , G01N33/574 , G01N33/58 , G06F19/00 , G01N
CPC分类号： G01N33/543 , C12Q1/6806 , C12Q1/6886 , G01N33/582 , G01N2458/40 , Y10S435/81 , Y10S435/973 , Y10S435/975 , Y10T436/145555 , Y10T436/24 , C12Q2563/167 , C12Q2563/137
摘要： The present invention provides compositions and methods for detecting, analyzing, and identifying biomolecules. More particularly, the invention provides Element Coded Affinity Tags comprising a metal chelate and a metal ion and methods of using the tags to detect, analyze, and identify biomolecules including polypeptides, nucleic acids, lipids, and polysaccharides.
摘要翻译：本发明提供用于检测，分析和鉴定生物分子的组合物和方法。更具体地，本发明提供了包含金属螯合物和金属离子的元件编码亲和标签，以及使用标签来检测，分析和鉴定包括多肽，核酸，脂质和多糖的生物分子的方法。

8. 发明申请

WO2003002974A2 ECL LABELS HAVING IMPROVED NON-SPECIFIC BINDING PROPERTIES, METHODS OF USING AND KITS CONTAINING THE SAME 审中-公开
标题翻译：具有改进的非特异性结合特性的ECL标签，使用方法和包含其的工具
公开(公告)号：WO2003002974A2
公开(公告)日：2003-01-09
申请号：PCT/US2002/019788
申请日：2002-06-21
申请人： IGEN INTERNATIONAL, INC.
发明人： SIGAL, George, B. , TJIONG, Howie , DONG, Liwen , MASOOD, Athar , TITMAS, Richard, C.
IPC分类号： G01N
CPC分类号： C07D213/30 , C07D213/34 , C07D213/40 , C07D213/81 , C07D471/04 , C07F9/582 , C07F9/6561 , C07F15/0053 , C09B57/00 , C09B57/10 , G01N33/582 , G01N2458/40
摘要： Bipyridine or phenanthroline ligands presenting functional groups that prevent non-specific binding (in particular, negatively charged functional groups that are unaffected by standard conditions for conjugating biological reagents through amide bonds) are described as are luminescent metal complexes comprising these ligands. The use of luminescent ruthenium and osmium complexes comprising these ligands in electrochemiluminescence assays shows that the use of these labels can significantly reduce the amount of non-specific binding observed relative to assays carried out using reagents labeled with analogous labels that don't present functional groups that decrease non-specific binding.
摘要翻译：描述了呈现阻止非特异性结合的官能团的联吡啶或菲咯啉配体（特别是不受标准条件影响的通过酰胺键缀合生物试剂的带负电荷的官能团）描述为包含这些配体的发光金属络合物。在电化学发光测定中使用包含这些配体的发光钌和锇络合物显示，使用这些标记物的使用可以显着减少相对于使用不具有官能团的类似标记标记的试剂进行的测定而观察到的非特异性结合的量这降低了非特异性结合。

9. 发明申请

WO0196877A2 NOVEL RARE EARTH METAL CRYPTATES WHICH ARE NOT VERY SENSITIVE TO THE FLUORESCENCE QUENCHING 审中-公开
标题翻译：对新鲜稀土金属泡沫非常敏感的新型稀土金属泡沫
公开(公告)号：WO0196877A2
公开(公告)日：2001-12-20
申请号：PCT/EP0106642
申请日：2001-06-12
申请人： CIS BIO INT , AUTIERO HERVE , BAZIN HERVE , MATHIS GERARD
发明人： AUTIERO HERVE , BAZIN HERVE , MATHIS GERARD
IPC分类号： G01N33/533 , C07D471/22 , G01N21/76 , G01N21/78 , G01N33/58
CPC分类号： G01N33/582 , C07D471/22 , G01N2458/40
摘要： The invention relates to a process for reducing the fluorescence quenching caused by the measuring medium in a fluorescence assay, by introducing into said medium of rare earth metal cryptates comprising at least one pyrdine radical which is substituted one or more times or unsubstituted. The invetion also relates to novel rare metal cryptates which are not very sensitive to the fluorescence quenching caused by the measuring medium.
摘要翻译：本发明涉及一种通过在所述介质中引入含有至少一个被取代一次或多次或未被取代的哒嗪基的稀土金属沉淀物来减少由荧光测定法中的测量介质引起的荧光猝灭的方法。这种侵袭还涉及对由测量介质引起的荧光猝灭不太敏感的新型稀有金属隐孢子虫。

10. 发明申请

WO01092882A1 METHOD OF DETECTING THE PRESENCE OF A LIQUID IN A MIX 审中-公开
标题翻译：检测液体在混合物中的存在方法
公开(公告)号：WO01092882A1
公开(公告)日：2001-12-06
申请号：PCT/IB2001/000944
申请日：2001-05-28
IPC分类号： G01N21/64 , G01N33/542 , G01N35/00 , G01N35/02 , G01N35/10
CPC分类号： G01N21/6428 , G01N33/542 , G01N35/00603 , G01N35/1009 , G01N2021/6439 , G01N2021/6443 , G01N2458/40
摘要： The invention relates to a method of detecting the presence of a liquid introduced into a mix of constituents. The method comprises the following steps: excitation of the mix by a light signal in the absence of the liquid to be detected; measurement of a fluorescence signal over a time DELTA t characteristic of the liquid to be detected, so as to produce a reference quantity Ro; introduction of an amount of liquid to be detected in the mix; excitation of the mix by a light signal; measurement of a fluorescence signal over a time DELTA t so as to produce a quantity R; comparison of the quantity R with a quantity kxRo. The invention applies to the detection of serum pipetting errors within the context of bioassays, especially immunological tests.
摘要翻译：本发明涉及检测引入到组分混合物中的液体的存在的方法。该方法包括以下步骤：在不存在待检测液体的情况下通过光信号激发混合物; 在待检测液体的时间DELTA t特性上测量荧光信号，以产生参考量Ro; 在混合物中引入待检测的液体量; 通过光信号激发混合物; 在一段时间内测量荧光信号DELTA t以产生量R; 数量R与数量kxRo的比较。本发明适用于在生物测定的上下文中检测血液移液误差，特别是免疫学测试。

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