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1. 发明申请

WO2006094360A1 METHOD OF AMPLIFYING NUCLEIC ACID 审中-公开
标题翻译：放大核酸的方法
公开(公告)号：WO2006094360A1
公开(公告)日：2006-09-14
申请号：PCT/AU2006/000318
申请日：2006-03-10
申请人： MOLECULAR PLANT BREEDING NOMINEES LTD , HAYDEN, Matthew, James
发明人： HAYDEN, Matthew, James
IPC分类号： C12Q1/68 , C12N15/00
CPC分类号： C12Q1/6851 , C12Q1/686 , C12Q2547/101 , C12Q2537/143 , C12Q2537/101 , C12Q2527/143 , C12Q2527/107 , C12Q2525/155
摘要： The present invention provides a method of amplifying nucleic acid using a PCR-based assay. For example, the present invention provides a method for amplifying a nucleic acid in a single closed-tube, the method comprising performing a first round amplification with an amount of a tagged locus-specific primer or set thereof suitable for performing exhaustive PCR. In a second round amplification, the first round amplification product is amplified using tag primers having lower melting temperature than the tagged locus-specific primers and annealed to the incorporated tag sequence in said first round amplification product at a lower annealing temperature than used in the first round. The present invention also provides a multiplex method of amplification. The present method also provides methods of characterising or identifying individuals or for diagnosing a disease or disorder.
摘要翻译：本发明提供使用基于PCR的测定来扩增核酸的方法。例如，本发明提供了用于在单个封闭管中扩增核酸的方法，所述方法包括用适于进行穷尽PCR的一定量的标记基因座特异性引物或其组合进行第一轮扩增。在第二轮扩增中，使用具有比标记的基因座特异性引物低的融合温度的标签引物来扩增第一轮扩增产物，并且在所述第一轮扩增产物中以比在第一轮扩增产物中使用的退火温度更低的退火温度退火回合。本发明还提供了扩增的多重方法。本方法还提供了表征或鉴定个体或用于诊断疾病或病症的方法。

2. 发明申请

WO2009036514A3 METHOD OF AMPLIFYING NUCLEIC ACID 审中-公开
标题翻译：放大核酸的方法
公开(公告)号：WO2009036514A3
公开(公告)日：2009-06-04
申请号：PCT/AU2008001396
申请日：2008-09-19
申请人： MOLECULAR PLANT BREEDING NOMINEES LTD , HAYDEN MATTHEW JAMES , TABONE TANIA
发明人： HAYDEN MATTHEW JAMES , TABONE TANIA
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6858 , C12Q2549/119 , C12Q2535/125
摘要： The present invention provides a method for detecting a polymorphism or mutation in nucleic acid comprising a first phase to amplify or enrich for a sequence comprising a polymorphism or mutation and a second phase for detecting the polymorphism or mutation, wherein both phases are performed in the same reaction vessel.
摘要翻译：本发明提供了一种检测核酸多态性或突变的方法，其包括第一相以扩增或富集包含多态性或突变的序列和用于检测多态性或突变的第二相，其中两相进行相同反应容器

3. 发明申请

WO2009036514A2 METHOD OF AMPLIFYING NUCLEIC ACID 审中-公开
标题翻译：放大核酸的方法
公开(公告)号：WO2009036514A2
公开(公告)日：2009-03-26
申请号：PCT/AU2008/001396
申请日：2008-09-19
申请人： MOLECULAR PLANT BREEDING NOMINEES LTD , HAYDEN, Matthew James , TABONE, Tania
发明人： HAYDEN, Matthew James , TABONE, Tania
CPC分类号： C12Q1/6858 , C12Q2549/119 , C12Q2535/125
摘要： The present invention provides a method for detecting a polymorphism or mutation in nucleic acid comprising a first phase to amplify or enrich for a sequence comprising a polymorphism or mutation and a second phase for detecting the polymorphism or mutation, wherein both phases are performed in the same reaction vessel.
摘要翻译：本发明提供了用于检测核酸中的多态性或突变的方法，所述方法包括扩增或富集包含多态性或突变的序列的第一阶段和用于检测多态性或突变的第二阶段，其中两个相在相同的反应容器中进行。

4. 发明申请

WO2009121091A1 MAPPING METHOD FOR POLYPLOID SUBJECTS 审中-公开
标题翻译：聚对苯二甲酸乙二酯的映射方法
公开(公告)号：WO2009121091A1
公开(公告)日：2009-10-08
申请号：PCT/AU2008/001397
申请日：2008-09-19
申请人： MOLECULAR PLANT BREEDING NOMINEES LTD , HAYDEN, Matthew James , TABONE, Tania
发明人： HAYDEN, Matthew James , TABONE, Tania
IPC分类号： A01H5/00 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , G06F19/22
摘要： The present invention provides a method for identifying a polymorphism or mutation in a genome of a polyploid subject, comprising comparing sequences within a target nucleic acid from genomes of two or more polyploid subjects, and determining linkage between one or more variable nucleotides in the sequences and one or more nucleotides within a specific genome of a polyploid subject, thereby identifying a polymorphism or mutation in a genome of a polyploid subject. The present invention also provides a method for producing a probe or primer comprising a sequence capable of preferentially or specifically annealing or hybridizing to a nucleic acid comprising a polymorphism or mutation identified by the method of the invention. The present invention also provides for the use of the method to determine variation in populations, associations between polymorphisms or mutations and specific traits, and in breeding.
摘要翻译：本发明提供了一种用于鉴定多倍体受试者的基因组中的多态性或突变的方法，其包括比较来自两个或多个多倍体受试者的基因组的靶核酸内的序列，以及确定序列中一个或多个可变核苷酸与在多倍体受试者的特定基因组内的一个或多个核苷酸，从而鉴定多倍体受试者的基因组中的多态性或突变。本发明还提供了一种用于生产探针或引物的方法，其包括能够优先或特异性退火或杂交的核酸的序列，所述核酸包含通过本发明的方法鉴定的多态性或突变。本发明还提供了该方法用于确定人群变异，多态性或突变之间的关联以及特定性状以及育种中的用途。

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