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    • 1. 发明申请
    • MAPPING METHOD FOR POLYPLOID SUBJECTS
    • 聚对苯二甲酸乙二酯的映射方法
    • WO2009121091A1
    • 2009-10-08
    • PCT/AU2008/001397
    • 2008-09-19
    • MOLECULAR PLANT BREEDING NOMINEES LTDHAYDEN, Matthew JamesTABONE, Tania
    • HAYDEN, Matthew JamesTABONE, Tania
    • A01H5/00C12Q1/68
    • C12Q1/6827C12Q1/6869G06F19/22
    • The present invention provides a method for identifying a polymorphism or mutation in a genome of a polyploid subject, comprising comparing sequences within a target nucleic acid from genomes of two or more polyploid subjects, and determining linkage between one or more variable nucleotides in the sequences and one or more nucleotides within a specific genome of a polyploid subject, thereby identifying a polymorphism or mutation in a genome of a polyploid subject. The present invention also provides a method for producing a probe or primer comprising a sequence capable of preferentially or specifically annealing or hybridizing to a nucleic acid comprising a polymorphism or mutation identified by the method of the invention. The present invention also provides for the use of the method to determine variation in populations, associations between polymorphisms or mutations and specific traits, and in breeding.
    • 本发明提供了一种用于鉴定多倍体受试者的基因组中的多态性或突变的方法,其包括比较来自两个或多个多倍体受试者的基因组的靶核酸内的序列,以及确定序列中一个或多个可变核苷酸与 在多倍体受试者的特定基因组内的一个或多个核苷酸,从而鉴定多倍体受试者的基因组中的多态性或突变。 本发明还提供了一种用于生产探针或引物的方法,其包括能够优先或特异性退火或杂交的核酸的序列,所述核酸包含通过本发明的方法鉴定的多态性或突变。 本发明还提供了该方法用于确定人群变异,多态性或突变之间的关联以及特定性状以及育种中的用途。