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    • 3. 发明申请
    • BIOCATALYTIC PROCESSES AND MATERIALS FOR ENHANCED CARBON UTILIZATION
    • 生物催化工艺和材料提高碳利用率
    • WO2017143194A2
    • 2017-08-24
    • PCT/US2017/018375
    • 2017-02-17
    • INVISTA NORTH AMERICA S.A.R.LINVISTA TECHNOLOGIES S.À.R.L.
    • SMITH, GaryCONRADIE, Alex, Van EckKIRBY, Gregory, S.PEARLMAN, Paul, S.
    • C12P5/00C12P5/02C12P7/24C12P13/00
    • C12P13/001C12M29/00C12M41/00C12N1/20C12P5/00C12P5/007C12P5/02C12P7/065C12P7/24C12P7/625C12P13/00C25B1/04F01D15/10F01K7/16F01K11/02F05D2220/31Y02E50/343Y02E60/366
    • The present disclosure describes biocatalytic processes for producing a product, comprising providing an aqueous stream (AS) comprising at least one fermentable substrate and a gaseous stream (GS) comprising at least one of CO 2 /H 2 , H 2 , methane, and/or CO to a fermentation zone, wherein the GS and AS stream are optionally contacted and/or mixed; the fermentation zone comprising at least one organism capable of metabolizing an AS substrate and a GS substrate, wherein the fermentation operates at conditions to mixotrophically metabolize at least one gaseous substrate in the GS and at least one substrate in the AS, producing the product. The present disclosure also describes compositions comprising an AS, a GS, and an organism, wherein the organism or an equivalent or engineered equivalent thereof is a methanotroph or a hydrogen-metabolizing or CO-metabolizing chemolithotrophic organism, and wherein the organism is capable of mixotrophic metabolism of at least one gaseous substrate in the GS and at least one substrate in the AS. The present disclosure also describes a process wherein said fermentation operates at conditions to mixotrophically metabolize at least H 2 in the gaseous stream and glycerol and lactic acid in the aqueous stream. The present disclosure also describes a system for producing a fermentation or bio-derived product.
    • 本公开描述了用于生产产物的生物催化方法,包括提供包含至少一种可发酵底物的水性流(AS)和包含CO 2中的至少一种的气态流(GS) 甲烷和/或CO到发酵区,其中所述GS和AS流任选地被接触和/或混合; 所述发酵区包含至少一种能够代谢AS底物和GS底物的生物体,其中所述发酵在使所述GS中的至少一种气态底物与所述AS中的至少一种底物混合营养代谢的条件下操作,产生所述产物。 本公开还描述了包含AS,GS和生物体的组合物,其中所述生物体或其等同物或工程等同物是甲烷氧化菌或氢代谢或CO代谢化学无机生物体,并且其中所述生物体能够混养 GS中的至少一种气态底物和AS中的至少一种底物的代谢。 本发明还描述了一种方法,其中所述发酵在使气态流中的至少H 2和甘油和水性流中的乳酸混合营养代谢的条件下操作。 本公开还描述了用于生产发酵或生物衍生产品的系统。
    • 4. 发明申请
    • ENGINEERED STRAIN OF ESCHERICHIA COLI FOR PRODUCTION OF POLY-R-3-HYDROXYALKANOATE POLYMERS WITH DEFINED MONOMER UNIT COMPOSITION AND METHODS BASED THEREON
    • 用于生产具有确定的单体单元组合物的聚-R-3-羟基丙酸酯聚合物的工程化大肠杆菌菌株及基于其的方法
    • WO2017087278A1
    • 2017-05-26
    • PCT/US2016/061596
    • 2016-11-11
    • THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK
    • NOMURA, Christopher T.PINTO, Atahualpa
    • C08G63/06C12N9/10C12P7/62
    • C08G63/06C08G63/6852C12N9/1029C12N9/88C12P7/625C12Y402/01119
    • Methods and systems for producing prescribed unit size azido-poly(3-hydroxyalkanoate) (azido-PHA) polymers and copolymers are provided. The methods and systems can employ recombinant bacteria that are not native producers of PHA or lack enzymes to degrade PHA once synthesized, metabolize short to long chain fatty acids without induction, and express an ( R )-specific enoyl-CoA hydratase and a PHA synthase, the ( R )-specific enoyl-CoA hydratase and PHA synthase having wide substrate specificities. The recombinant bacteria are fed at least one ω-azidofatty acid substrate that is equal in carbon length to the prescribed or desired unit size of an azido-PHA polymer to be produced. Azido-PHA polymers or azido-PHA co-polymers can be conjugated via copper-catalyzed alkyne-azide cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions. The prescribed unit size conjugated azido-PHA polymer or orthogonally conjugated azido-PHA co-polymer that is produced is then isolated and/or purified.
    • 提供了用于生产规定单位尺寸的叠氮基聚(3-羟基链烷酸酯)(叠氮基-PHA)聚合物和共聚物的方法和系统。 所述方法和系统可以使用不是PHA的天然生产者或者缺乏一旦合成就降解PHA的酶的重组细菌,在没有诱导的情况下代谢短链到长链脂肪酸,并且表达(R) - 特异性烯酰 -CoA水合酶和PHA合酶,(R)特异性烯醇-CoA水合酶和PHA合酶具有很宽的底物特异性。 将至少一种碳长度相等的ω-叠氮基脂肪酸底物加入到待生产的叠氮基-PHA聚合物的处方或所需单位尺寸中。 可通过铜催化的炔 - 叠氮环加成(CuAAC)或菌株促进的叠氮 - 炔环加成(SPAAC)反应将Azido-PHA聚合物或叠氮基-PHA共聚物缀合。 然后分离和/或纯化所产生的规定单位大小的共轭叠氮基-PHA聚合物或正交共轭叠氮基-PHA共聚物。