专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2019012058A1 CELL FACTORY HAVING IMPROVED IRON-SULFUR CLUSTER DELIVERY 审中-公开
公开(公告)号：WO2019012058A1
公开(公告)日：2019-01-17
申请号：PCT/EP2018/068989
申请日：2018-07-12
申请人： BIOSYNTIA APS
发明人： GENEE, Hans Jasper , BALI, Anne Pihl , MYLING-PETERSEN, Nils
IPC分类号： C07K14/245 , C12P17/18 , C12N9/10 , C12N9/88 , C12P7/40 , C12P17/16 , C12N1/21
摘要： The invention provides a genetically modified bacterial cell capable of improved iron-sulfur cluster delivery, characterized by a modified gene encoding a mutant Iron Sulfur Cluster Regulator (IscR) as well as one or more transgenes encoding polypeptides that enhance the biosynthesis of either biotin, lipoic acid or thiamine. The invention provides a method for producing either biotin, lipoic acid or thiamine using the genetically modified bacterium of the invention; as well as for the use of the genetically modified bacterial cell for either biotin, lipoic acid or thiamine production.

2. 发明申请

WO2017103221A1 A GENETICALLY MODIFIED BACTERIAL CELL FACTORY FOR THIAMINE PRODUCTION 审中-公开
标题翻译：一种经遗传修饰的细菌细胞工厂，用于生产亚胺
公开(公告)号：WO2017103221A1
公开(公告)日：2017-06-22
申请号：PCT/EP2016/081598
申请日：2016-12-16
申请人： BIOSYNTIA APS
发明人： GRONENBERG, Luisa , SALOMONSEN, Bo , FERLA, Matteo , GENEE, Hans Jasper
IPC分类号： C12N1/21 , C12N9/06 , C12N9/10 , C12N9/12 , C12N9/16 , C12N9/88 , C12N15/55 , C12P17/16
CPC分类号： C12N9/16 , C12N9/0022 , C12N9/1085 , C12N9/1229 , C12N9/1241 , C12N9/13 , C12N9/88 , C12P17/167 , C12Y301/03
摘要： The invention provides a genetically modified bacterium for production of thiamine; where the bacterium is characterized by a transgene encoding a thiamine monophosphate phosphatase (TMP phosphatase having EC 3.1.3.-) as well as transgenes encoding polypeptides that catalyze steps in the thiamine pathway. The genetically modified bacterium is characterized by enhanced synthesis and release of thiamine into the extracellular environment. The invention further provides a method for producing thiamine using the genetically modified bacterium of the invention; as well as the use of the genetically modified bacterium for extracellular thiamine production.
摘要翻译：本发明提供了用于生产硫胺素的遗传修饰的细菌; 其中细菌的特征在于编码硫胺素单磷酸磷酸酶（具有EC 3.1.3.-的TMP磷酸酶）的转基因以及编码在硫胺素途径中催化步骤的多肽的转基因。该基因修饰的细菌的特征在于增强了硫胺进入细胞外环境的合成和释放。本发明还提供了使用本发明的基因修饰细菌生产硫胺素的方法; 以及使用转基因细菌进行细胞外硫胺素生产。

3. 发明申请

WO2014187829A1 REGULATABLE GENE EXPRESSION 审中-公开
标题翻译：可调节的基因表达
公开(公告)号：WO2014187829A1
公开(公告)日：2014-11-27
申请号：PCT/EP2014/060353
申请日：2014-05-20
申请人： BIOSYNTIA APS
发明人： GENEE, Hans Jasper , SOMMER, Morten
IPC分类号： C12Q1/68 , C12N15/10 , C12N15/63 , C12N15/67 , C40B40/02 , C40B40/06
CPC分类号： C12N15/635 , C12N15/1093 , C12N15/63
摘要： The present invention relates to a regulatable gene expression construct comprising a nucleic acid molecule comprising two or more regulation sequences encoding respective RNA molecules comprising a riboswitch responsive to an effector compound, said riboswitch being operably linked to a respective coding region which encodes a respective modulator compound for modulating the action of a respective growth regulator compound and each said riboswitch in each regulation sequence being selected be responsive to the same effector compound to trigger expression of its respective modulator compound. The invention also relates to a method of using the regulatable gene expression construct for selecting from a metagenomic library a primary modulator compound which effects a chemical transformation of a substrate into said effector compound or transports said effector compound into a micro-organism comprising the regulatable gene expression construct.
摘要翻译：本发明涉及可调控基因表达构建体，其包含核酸分子，其包含两个或多个编码各自RNA分子的调节序列，所述调节序列包含响应于效应化合物的核糖开关，所述核糖开关可操作地连接到编码相应调节剂化合物的相应编码区用于调节各生长调节剂化合物的作用，并且每个调节序列中的每个所述核糖开关被选择以响应相同的效应子化合物以触发其各自的调节剂化合物的表达。本发明还涉及使用可调控基因表达构建体从宏基因组文库中选择一种主要调节剂化合物，其将底物化学转化成所述效应化合物或将所述效应子化合物转运到包含可调控基因的微生物中表达式构造。

4. 发明申请

WO2023285585A2 MICROBIAL CELL FACTORIES PRODUCING VITAMIN B COMPOUNDS 审中-公开
公开(公告)号：WO2023285585A2
公开(公告)日：2023-01-19
申请号：PCT/EP2022/069711
申请日：2022-07-14
申请人： BIOSYNTIA APS
发明人： ACEVEDO-ROCHA, Carlos, Guillermo , GRONENBERG, Luisa, Simona , GENEE, Hans Jasper
IPC分类号： C12N1/20 , C12N15/52 , C07K14/245 , C12N9/88 , C12P17/167 , C12P17/186 , C12P19/42 , C12R2001/19 , C12Y406/01001
摘要： The present disclosure relates to a genetically modified host cell having increased production of one or more vitamin B compounds, wherein the host cell is genetically modified by mutating one or more native polynucleotide constructs for reducing formation of a CRP-cAMP complex in the host cell and/or introducing one or more genetic alterations increasing the degradation and/or non-CRP binding of cAMP in the host cell; whereby the production of the vitamin B compound in the genetically modified host cell is increased compared to a parent host cell.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式