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1. 发明申请

WO2014066481A1 METHODS AND KITS FOR DETECTION OF A PATHOGEN IN SUGARCANE 审中-公开
标题翻译：用于检测糖尿病的方法和试剂盒
公开(公告)号：WO2014066481A1
公开(公告)日：2014-05-01
申请号：PCT/US2013/066338
申请日：2013-10-23
申请人： SYNGENTA PARTICIPATIONS AG , FAN, Chunyang , YU, Wenjin , CORREA, Natassia , ROSA, Daniel Dias , WOUDT, Lambertus Pieter , SAINZ, Manuel Benito , WHITE, Kimberly Ann
发明人： FAN, Chunyang , YU, Wenjin , CORREA, Natassia , ROSA, Daniel Dias , WOUDT, Lambertus Pieter , SAINZ, Manuel Benito , WHITE, Kimberly Ann
IPC分类号： C12Q1/04
CPC分类号： C12Q1/689 , C12Q1/04
摘要： Embodiments of the present invention provide a diagnostic approach utilizing quantitative polymerase chain reaction (PCR) to detect quantitatively a pathogen of the genus Leifsonia that causes ratoon stunting disease (RSD) in sugarcane. This is a rapid, cost-effective and/or high sensitivity methodology for detecting this pathogen. The present invention relates to methods and kits for detecting a pathogen in a plant, plant part or plant cell from the Gramineae/Poaceae family, such as plants of the Saccharum spp, including sugarcane.
摘要翻译：本发明的实施方案提供了一种利用定量聚合酶链反应（PCR）定量检测引起甘蔗中的oon发育迟缓病（RSD）的雷公氏菌属的病原体的诊断方法。这是用于检测该病原体的快速，成本效益和/或高灵敏度的方法。本发明涉及用于检测来自禾本科/禾本科家族的植物，植物部分或植物细胞中的病原体的方法和试剂盒，例如甘蔗属植物（包括甘蔗）的植物。

2. 发明申请

WO03048319A3 NUCLEOTIDE SEQUENCES ENCODING PROTEINS CONFERRING ABIOTIC STRESS TOLERANCE IN PLANTS 审中-公开
标题翻译：核糖体序列编码在植物中承认生物胁迫耐性的蛋白质
公开(公告)号：WO03048319A3
公开(公告)日：2003-08-14
申请号：PCT/US0238359
申请日：2002-11-27
申请人： SYNGENTA PARTICIPATIONS AG , SAINZ MANUEL B , SALMERON JOHN , WEISLO LAURA
发明人： SAINZ MANUEL B , SALMERON JOHN , WEISLO LAURA
IPC分类号： C07K14/415 , C07K14/47 , C12N15/29 , C12N15/82 , C12N5/04 , A01H1/00 , A01H5/00 , A01H5/10 , C07K14/00 , C12N15/09 , C12N15/52 , C12N15/63 , C12N15/87 , C12N15/90
CPC分类号： C07K14/415 , C07K14/47 , C12N15/8243 , C12N15/8261 , C12N15/8271 , C12N15/8279 , Y02A40/146
摘要： The present invention encompasses nucleic acid molecules isolated from Oryza sativa that encode proteins for conferring abiotic stress tolerance, enhanced yield, disease resistance, or altered nutritional composition in plants. The invention further relates to expression of these molecules in microorganisms and transgenic plants for altering these characteristics of the organism.
摘要翻译：本发明包括从水稻分离的核酸分子，其编码用于赋予非生物胁迫耐受性，增强的产量，抗病性或植物中改变的营养组成的蛋白质。本发明还涉及这些分子在微生物和转基因植物中的表达，以改变生物体的这些特征。

3. 发明申请

WO2003048339A2 NUCLEIC ACID MOLECULES FROM RICE ENCODING RAR1 DISEASE RESISTANCE PROTEINS AND USES THEREOF 审中-公开
标题翻译：来自大米编码RAR1抗病性蛋白质的核酸分子及其用途
公开(公告)号：WO2003048339A2
公开(公告)日：2003-06-12
申请号：PCT/US2002/039030
申请日：2002-11-27
申请人： SYNGENTA PARTICIPATIONS AG , SAINZ, Manuel, B. , SALMERON, John
发明人： SAINZ, Manuel, B. , SALMERON, John
IPC分类号： C12N
CPC分类号： C12N15/8281 , C07K14/415 , C12N15/8279 , C12N15/8282
摘要： The present invention pertains to nucleic acid molecules isolated from Orysa sativa comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypetides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention. The invention also relates to methods of cloning nucleic acid molecules encoding RAR1 proteins using polymerase chain reaction and primers of the invention.
摘要翻译：本发明涉及从包含编码参与抗病性的RAR1蛋白的核苷酸序列的Orysa sativa分离的核酸分子和RAR1多肽。本发明特别涉及改变转基因植物中编码RAR1蛋白的表达核酸分子以改变水平的抗病性的方法，以及改变增强的抗病性的转基因植物，后代和种子。本发明还涉及增强R抗性基因表达，抗病性信号转导基因，涉及介导抗病性或参与抗病性分子合成的基因的方法。本发明还涉及通过增加本发明的核酸分子的表达来调节其它编码序列的表达的方法。本发明还涉及使用本发明的聚合酶链反应和引物克隆编码RAR1蛋白的核酸分子的方法。

4. 发明申请

WO2014066481A8 METHODS AND KITS FOR DETECTION OF A PATHOGEN IN SUGARCANE 审中-公开
标题翻译：用于检测糖尿病的方法和试剂盒
公开(公告)号：WO2014066481A8
公开(公告)日：2015-05-21
申请号：PCT/US2013066338
申请日：2013-10-23
申请人： SYNGENTA PARTICIPATIONS AG , FAN CHUNYANG , YU WENJIN , CORREA NATASSIA , ROSA DANIEL DIAS , WOUDT LAMBERTUS PIETER , SAINZ MANUEL BENITO , WHITE KIMBERLY ANN
发明人： FAN CHUNYANG , YU WENJIN , CORREA NATASSIA , ROSA DANIEL DIAS , WOUDT LAMBERTUS PIETER , SAINZ MANUEL BENITO , WHITE KIMBERLY ANN
IPC分类号： C12Q1/04
CPC分类号： C12Q1/689 , C12Q1/04
摘要： Embodiments of the present invention provide a diagnostic approach utilizing quantitative polymerase chain reaction (PCR) to detect quantitatively a pathogen of the genus Leifsonia that causes ratoon stunting disease (RSD) in sugarcane. This is a rapid, cost-effective and/or high sensitivity methodology for detecting this pathogen. The present invention relates to methods and kits for detecting a pathogen in a plant, plant part or plant cell from the Gramineae/Poaceae family, such as plants of the Saccharum spp, including sugarcane.
摘要翻译：本发明的实施方案提供了一种利用定量聚合酶链反应（PCR）定量检测引起甘蔗中的oon发育迟缓病（RSD）的雷公氏菌属的病原体的诊断方法。这是用于检测该病原体的快速，成本效益和/或高灵敏度的方法。本发明涉及用于检测来自禾本科/禾本科家族的植物，植物部分或植物细胞中的病原体的方法和试剂盒，例如甘蔗属植物（包括甘蔗）的植物。

5. 发明申请

WO2010151634A1 METHODS FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF SUGAR CANE 审中-公开
标题翻译：农杆菌介导的蔗糖转化方法
公开(公告)号：WO2010151634A1
公开(公告)日：2010-12-29
申请号：PCT/US2010/039774
申请日：2010-06-24
申请人： SYNGENTA PARTICIPATIONS AG , DE LUCCA, Paulo, Cezar , DONG, Shujie , GEIJSKES, Robert, Jason, Christopher , DUNDER, Erik, Martin , SAINZ, Manuel, Benito
发明人： DE LUCCA, Paulo, Cezar , DONG, Shujie , GEIJSKES, Robert, Jason, Christopher , DUNDER, Erik, Martin , SAINZ, Manuel, Benito
IPC分类号： C12N15/87 , A01H5/00 , A01H1/00
CPC分类号： C12N15/8205
摘要： The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with an Agrobacterium inoculation suspension, said Agrobacterium comprising a nucleic acid of interest, to obtain an Agrobacterium -inoculated sugar cane tissue or cell thereof; b) co-cultivating said Agrobacterium -inoculated sugar cane tissue or cell thereof on a surface in the absence of co-culture media for a time period sufficient to reduce original weight of said Agrobacterium -inoculated-inoculated sugar cane tissue or cell thereof; and c) selecting a transformed sugar cane tissue or a cell thereof comprising said nucleic acid of interest. The transformation methods of the invention provide for increased transformation frequency and recovery of transgenic sugar cane plants.
摘要翻译：本发明提供了生产转化的甘蔗组织或其细胞的方法，所述方法包括：a）用农杆菌接种悬浮液接种甘蔗组织或其细胞，所述农杆菌包含感兴趣的核酸，以获得农杆菌接种的甘蔗组织或其细胞; b）在不存在共培养基的情况下，将所述农杆菌接种的甘蔗组织或其细胞共培养一段足以减少所述农杆菌接种的甘蔗组织或其细胞的原始重量的时间段; 和c）选择转化的甘蔗组织或其包含所述感兴趣的核酸的细胞。本发明的转化方法提供了转基因甘蔗植物的转化频率和恢复的增加。

6. 发明申请

WO2002102430A1 METODO PARA LA OBTENCION DE IMPLANTES BIOACTIVOS UTILES COMO SISTEMAS DE LIBERACION CONTROLADA DE ANTIBIOTICOS 审中-公开
标题翻译：用于生产用于控制释放抗生素的系统的生物活性植入物的方法
公开(公告)号：WO2002102430A1
公开(公告)日：2002-12-27
申请号：PCT/ES2002/000301
申请日：2002-06-14
申请人： UNIVERSIDAD COMPLUTENSE DE MADRID RECTORADO , VALLET REGI, Maria , ARCOS NAVARRETE, Daniel , CLAVEL SAINZ, Manuel , MESEGUER OLMO, Luis , RAGEL PRUDENCIO, Carmen, Victoria
发明人： VALLET REGI, Maria , ARCOS NAVARRETE, Daniel , CLAVEL SAINZ, Manuel , MESEGUER OLMO, Luis , RAGEL PRUDENCIO, Carmen, Victoria
IPC分类号： A61L27/12
CPC分类号： A61L27/10 , A61L27/54 , A61L2300/406 , A61L2300/602
摘要： Método para la obtención de implantes bioactivos útiles como sistemas de liberación controlada de antibióticos. La invención consiste en un método en el que se lleva a cabo el control del tamaño de grano de un vidrio bioactivo, la mezcla de dicho vidrio con un antibiótico, el conformado de la mezcla aplicando presión de tipo uniaxial y posterior prensado isostático. Se obtienen piezas compactas para ser implantadas en el tejido óseo, capaces de proporcionar dosis efectivas de antibiótico a nivel local. Los implantes presentan capacidad para promover la regeneración ósea. De aplicación en cirugía ortopedica para relleno de defectos óseos de cualquier etiología y en la prevención y tratamiento de infecciones óseas de origen bacteriano.
摘要翻译：本发明涉及生物活性植入物的制备方法，其包括：控制生物活性玻璃的晶粒尺寸; 将所述玻璃与抗生素混合; 通过施加单轴压力形成由此获得的混合物; 并随后使混合物经受等静压。以这种方式，可以获得可以植入骨组织中并可局部施用有效剂量的抗生素的紧凑件。本发明的植入物也可以刺激骨再生。所述植入物可用于骨科手术中以填充任何细菌性骨缺损以及用于预防和治疗细菌来源的骨感染。

7. 发明申请

WO2012099664A1 METHODS AND COMPOSITIONS FOR MODIFIED ETHANOL INDUCIBLE PROMOTER SYSTEMS 审中-公开
标题翻译：改性乙醇可溶性促进剂体系的方法和组合
公开(公告)号：WO2012099664A1
公开(公告)日：2012-07-26
申请号：PCT/US2011/065184
申请日：2011-12-15
申请人： SYNGENTA PARTICIPATIONS AG , QUEENSLAND UNIVERSITY OF TECHNOLOGY , SAINZ, Manuel , KINKEMA, Mark David
发明人： SAINZ, Manuel , KINKEMA, Mark David
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12N15/8238 , C12N15/113 , C12N15/8241
摘要： The present invention provides nucleic acid molecules comprising one or more nucleotide sequences selected from the group consisting of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and/or SEQ ID NO:10 that can be operably linked to a promoter, thereby making the promoter inducible by a chemical compound that can induce the expression of the alcohol dehydrogenase system of Aspergillus nidulans . Methods for making an inducible promoter and for making the expression of a nucleotide sequence of interest inducible are also provided. Further provided are plants, plant parts, and plant cells, comprising the compositions of the present invention.
摘要翻译：本发明提供了包含一个或多个选自下组的核酸序列的核酸分子：SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：：6，SEQ ID NO：7，SEQ ID NO：8和/或SEQ ID NO：10，其可以可操作地连接到启动子，从而使启动子可诱导可诱导醇脱氢酶表达的化学化合物构巢曲霉系统。还提供了制备诱导型启动子和用于诱导感兴趣的核苷酸序列的表达的方法。还提供植物，植物部分和植物细胞，其包含本发明的组合物。

8. 发明申请

WO2003048339A3 NUCLEIC ACID MOLECULES FROM RICE ENCODING RAR1 DISEASE RESISTANCE PROTEINS AND USES THEREOF 审中-公开
公开(公告)号：WO2003048339A3
公开(公告)日：2003-06-12
申请号：PCT/US2002/039030
申请日：2002-11-27
申请人： SYNGENTA PARTICIPATIONS AG , SAINZ, Manuel, B. , SALMERON, John
发明人： SAINZ, Manuel, B. , SALMERON, John
IPC分类号： C12N15/09
摘要： The present invention pertains to nucleic acid molecules isolated from Orysa sativa comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypetides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention. The invention also relates to methods of cloning nucleic acid molecules encoding RAR1 proteins using polymerase chain reaction and primers of the invention.

9. 发明申请

WO2003048319A2 NUCLEIC ACID MOLECULES FROM RICE ENCODING PROTEINS FOR ABIOTIC STRESS TOLERANCE, ENHANCED YIELD, DISEASE RESISTANCE AND ALTERED NUTRITIONAL QUALITY AND USES THEREOF 审中-公开
标题翻译：来自大米编码蛋白质的核酸分子，用于抗生素耐受性，增强的耐药性，抗病性和改变的营养质量及其用途
公开(公告)号：WO2003048319A2
公开(公告)日：2003-06-12
申请号：PCT/US2002/038359
申请日：2002-11-27
申请人： SYNGENTA PARTICIPATIONS AG , SAINZ, Manuel, B. , SALMERON, John , WEISLO, Laura
发明人： SAINZ, Manuel, B. , SALMERON, John , WEISLO, Laura
IPC分类号： C12N
CPC分类号： C07K14/415 , C07K14/47 , C12N15/8243 , C12N15/8261 , C12N15/8271 , C12N15/8279 , Y02A40/146
摘要： The present invention encompasses nucleic acid molecules isolated from Oryza sativa that encode proteins for conferring abiotic stress tolerance, enhanced yield, disease resistance, or altered nutritional composition in plants. The invention further relates to expression of these molecules in microorganisms and transgenic plants for altering these characteristics of the organism.
摘要翻译：本发明包括从水稻分离的核酸分子，其编码用于赋予非生物胁迫耐受性，增强的产量，抗病性或植物中改变的营养组成的蛋白质。本发明还涉及这些分子在微生物和转基因植物中的表达，以改变生物体的这些特征。

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