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    • 6. 发明申请
    • METHOD FOR ISOLATION OF BULK ANTHOCYANIDINS AND OTHER BIOACTIVES
    • 分离大肠杆菌和其他生物活性的方法
    • WO2015127118A1
    • 2015-08-27
    • PCT/US2015/016678
    • 2015-02-19
    • GUPTA, Ramesh, C.
    • GUPTA, Ramesh, C.
    • C07D311/62C07D311/76A61K36/45C07H17/06
    • C07D311/76A23L5/43C07D311/62
    • This invention describes a simple, rapid and cost-effective method to isolate bulk quantities of relatively pure and enriched anthocyanidins and other plant bioactives. The method is based on the principle of solubility. Some bioactives (anthocyanidins) were extracted in an aqueous solvent, transferred to a non-aqueous solvent and finally insolubilized by adding a miscible solvent in which the bioactive was insoluble. Thus, anthocyanidins were isolated from anthocyanin-enriched berries or non-enriched, dark-colored fruits, vegetables and grains by extraction of anthocyanins, acid hydrolysis, and extraction of the resulting anthocyanidins, followed by their insolubilization (precipitation). Some bioactives (hydrophobic and hydrophilic) were extracted in a solvent with high solubility and then directly insolubilized by adding a miscible solvent in which the bioactive was insoluble, for example, withaferin A from enriched Withania somnifera and punicalagins from enriched punica extract.
    • 本发明描述了分离大量相对纯的和富集的花青素和其它植物生物活性物的简单,快速和成本有效的方法。 该方法是基于溶解度的原理。 一些生物活性物质(花色素苷)在水性溶剂中提取,转移到非水溶剂中,最后通过加入其中生物活性不溶性的混溶溶剂而不溶解。 因此,通过提取花青素,酸水解和提取所得花青素,然后使其不溶(沉淀),从花青素浓缩的浆果或非浓缩的深色水果,蔬菜和谷物中分离出花青素。 一些生物活性物质(疏水性和亲水性)在高溶解度的溶剂中提取,然后通过加入其中生物活性不溶性的可混溶溶剂直接不溶,例如来自富集的有色金属藻的嗜铬蛋白A和来自浓缩的竹提提液的刺梧桐木。
    • 10. 发明申请
    • ANTHOCYANIN-BASED COLORANT
    • 基于蒽醌的颜料
    • WO2013079518A1
    • 2013-06-06
    • PCT/EP2012/073812
    • 2012-11-28
    • CHR. HANSEN A/S
    • MANE, CarineCHANFORAN, CélineLEMMONIER, PatrickJOUENNE, Eric
    • C09B61/00A23L1/27
    • A23G9/42A23G3/48A23L2/58A23L5/43A23L19/00C09B61/00
    • 1. A composition, which is an anthocyanin-based colorant composition comprising 50-90 mol-%, based on the total amount of anthocyanins, of pelargonidin-based anthocyanins, and wherein (i) > 70 mol-% of all anthocyanins are acylated with at least one phenolic acid; and (ii) > 20 mol-% of all anthocyanins are acylated with at least one hydroxycinnamic acid; and wherein the composition has a red color with a hue value H ≥ in the L*C*h* color system in the range of 10-30, measured at an L*-value of (70.0 ± 0.1) in a 0.1 mol/l trisodium citrate dihydrate buffer at pH 3 in a 1 cm-length quartz cell using Spectraflash 650 (Datacolor) in transmission mode under D65 illuminant 10 Deg. Also, the present invention relates to use of the above compositions as a food colorant.
    • 1.一种组合物,其是基于花青素总量的基于花青素的花青素的50-90mol%的基于花青素的花青素,并且其中(i)> 70mol%的所有花青素被酰化 与至少一种酚酸; 和(ii)> 20mol%的所有花青素用至少一种羟基肉桂酸酰化; 并且其中所述组合物在L * C * h *颜色体系中具有在10-30的范围内的色调值H> =的红色,在(70.0±0.1)的L *值下以0.1mol / l三磷酸三钠缓冲液,在1cm长的石英池中,使用Spectraflash 650(Datacolor)在透射模式下,在D65光源下10度。 此外,本发明涉及上述组合物作为食品着色剂的用途。