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    • 3. 发明申请
    • PROCESS FOR SEPARATING LIPID MATERIALS
    • 分离脂肪材料的方法
    • WO2007123424A1
    • 2007-11-01
    • PCT/NZ2007/000087
    • 2007-04-20
    • CATCHPOLE, Owen John,TALLON, Stephen John,
    • CATCHPOLE, Owen John,TALLON, Stephen John,
    • C11B7/00C11B1/10A23L1/48
    • C11B7/005A23C2240/05A23J7/00A23L5/23A23V2002/00A23V2300/18A23V2300/44
    • The present invention relates to processes for separating a feed material into soluble and insoluble components, by contacting a feed material and a solvent and subsequently separating the solvent containing the soluble components from the insoluble components, wherein the feed material comprises one or more of: at least 1% by mass phosphatidyl serine, at least 1% by mass sphingomyelin, at least 0.3 % by mass acylalkylphospholipids and/or plasmalogens, at least 0.5 % by mass aminoethylphosphonate and/or other phosphonolipids, at least 1% by mass cardiolipin, and at least 0.3% by mass gangliosides; and wherein the solvent comprises: supercritical or near-critical CO 2 , and a co-solvent comprising one or more C 1 -C 3 monohydric alcohols, and water, wherein the co-solvent makes up at least 10% by mass of the CO 2 , and the water content of the co-solvent is 0 to 40 % by mass. The present invention also relates to processes for separating a feed material into soluble and insoluble components, comprising contacting a feed material and a first solvent and subsequently separating the first solvent containing the first soluble components from the first insoluble components, wherein the feed material comprises one or more of: at least 1 % by mass phosphatidyl serine, at least 1% by mass sphingomyelin, at least 0.3 % by mass acylalkylphospholipids and/or plasmalogens, at least 0.5 % by mass aminoethylphosphonate and/or other phosphonolipids, at least 1% by mass cardiolipin, or at least 0.3% by mass gangliosides; and wherein the first solvent comprises supercritical or near-critical CO 2 . The process then provides contacting the first insoluble components with a second solvent and subsequently separating the second solvent containing the second soluble components from the second insoluble components, wherein the second solvent comprises supercritical or near- critical CO 2 , and a co-solvent comprising one or more C 1 -C 3 monohydric alcohols, and water, wherein the co-solvent makes up at least 10% by mass of the CO 2, and the water content of the co-solvent is 0 to 40% by mass.
    • 本发明涉及通过使进料和溶剂接触并随后将含有可溶性组分的溶剂与不溶性组分分离而将进料分离成可溶和不溶组分的方法,其中所述进料包括以下中的一种或多种: 至少1质量%的磷脂酰丝氨酸,至少1质量%的鞘磷脂,至少0.3质量%的酰基烷基磷脂和/或等离子体发生剂,至少0.5质量%的氨基乙基膦酸盐和/或其它磷脂脂质,至少1质量%的心磷脂,和 至少0.3%质量的神经节苷脂; 并且其中所述溶剂包括:超临界或近临界CO 2 2,以及包含一个或多个C 1 -C 3 -C 3一元的共溶剂 醇和水,其中共溶剂占CO 2的至少10质量%,共溶剂的水含量为0〜40质量%。 本发明还涉及用于将进料分离成可溶性和不溶性组分的方法,包括使进料和第一溶剂接触并随后将含有第一可溶组分的第一溶剂与第一不溶组分分离,其中进料包含一种 至少1质量%的磷脂酰丝氨酸,至少1质量%的鞘磷脂,至少0.3质量%的酰基烷基磷脂和/或等离子体发生剂,至少0.5质量%的氨基乙基膦酸酯和/或其它磷脂脂,至少1重量% 或者至少0.3质量%的神经节苷脂; 并且其中所述第一溶剂包括超临界或近临界CO 2。 然后该方法使第一不溶组分与第二溶剂接触,随后将含有第二可溶组分的第二溶剂与第二不溶组分分离,其中第二溶剂包括超临界或近临界CO 2, 和包含一种或多种C 1 -C 3 - 一元醇的共溶剂和水,其中所述助溶剂占所述CO的至少10质量% 共溶剂的水分含量为0〜40质量%。
    • 7. 发明申请
    • METHOD OF DETECTING EXPRESSION OF AND ISOLATING THE PROTEIN ENCODED BY THE BRCA1 GENE
    • 检测BRCA1基因编码的蛋白质的表达和分离方法
    • WO1998008394A1
    • 1998-03-05
    • PCT/US1997014515
    • 1997-08-19
    • CORNELL RESEARCH FOUNDATION, INC.
    • CORNELL RESEARCH FOUNDATION, INC.SPITSBERG, Vitaly, L.GOREWIT, Ronald, C.
    • A23C13/00
    • G01N33/57488A23C2240/05C07K14/4702G01N33/57415G01N2333/47G01N2333/4703G01N2333/82Y10S436/813Y10S530/827Y10S530/832
    • The present discloser teaches that the proteins encoded by the BRCA1 and BRCA2 genes are found in the milk fat globule membranes from humans and cows. Therefore, BRCA1 and BRCA2 proteins can be isolated from milk produced by lactating animals. The level of expression of BRCA1 or BRCA2 can be determined by sampling the levels of BRCA1 or BRCA2 proteins found in the MFGM of lactating animals. The present invention also includes a method of determining the likelihood that a woman will develop breast cancer by measuring the amount of the BRCA1 or BRCA2 expression during lactation. The detection of expression of BRCA1 or BRCA2 can be accomplished with an antibody raised specifically against BRCA1 or BRCA2 proteins, by isolating BRCA1 or BRCA2 from the milk fat globule membranes or by detecting activity of BRCA1 or BRCA2. The level of BRCA1 or BRCA2 is compared to a reference scale of propensity for breast cancer development correlated to normally active BRCA1 or BRCA2 levels. BRCA1, BRCA2, fatty acid binding proteins and phosphorylated proteins are found in the MFGM and the MFGM can be isolated from milk and provided in a form suitable for oral consumption, i.e. a tablet or capsule of separated milk fat globule membranes, a food additive, or a fortified dairy commodity.
    • 本发明人教导了由BRCA1和BRCA2基因编码的蛋白质存在于人和牛的乳脂肪球膜中。 因此,BRCA1和BRCA2蛋白可以从哺乳动物产生的乳汁中分离出来。 可以通过对哺乳动物的MFGM中发现的BRCA1或BRCA2蛋白的水平进行取样来确定BRCA1或BRCA2的表达水平。 本发明还包括一种通过测量哺乳期BRCA1或BRCA2表达量来确定女性会发展成乳腺癌的可能性的方法。 BRCA1或BRCA2的表达检测可以通过用BRCA1或BRCA2蛋白特异性产生的抗体,通过从乳脂肪球膜分离BRCA1或BRCA2或通过检测BRCA1或BRCA2的活性来完成。 将BRCA1或BRCA2的水平与与正常活动的BRCA1或BRCA2水平相关的乳腺癌发展倾向的参考量表进行比较。 在MFGM中发现BRCA1,BRCA2,脂肪酸结合蛋白和磷酸化蛋白质,并且MFGM可以从牛奶中分离并以适合于口服消费的形式提供,即分离的乳脂肪球膜的片剂或胶囊,食品添加剂, 或强化乳制品。