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1. 发明申请

WO2003048395A1 METHODS FOR THE SELECTION AND CLONING OF NUCLEIC ACID MOLECULES FREE OF UNWANTED NUCLEOTIDE SEQUENCE ALTERATIONS 审中-公开
标题翻译：不含核苷酸序列变异的核酸分子的选择和克隆方法
公开(公告)号：WO2003048395A1
公开(公告)日：2003-06-12
申请号：PCT/US2002/038467
申请日：2002-12-03
申请人： ZYMOGENETICS, INC.
发明人： LOK, Si , TANNHEIMER, Stacey
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2521/325 , C12Q2563/131
摘要： The accurate synthesis of nucleic acid molecules is important for use of amplified nucleic acid molecules as hybridization probes, in the regulation of gene expression, as templates for the production of recombinant proteins, as diagnostic probes, and in forensic analyses. Methods are provided to separate nucleic acid molecules that are free of mutations from a population of nucleic acid molecules that contain unwanted nucleotide alternations.
摘要翻译：核酸分子的精确合成对于使用扩增的核酸分子作为杂交探针，调节基因表达，作为生产重组蛋白的模板，作为诊断探针，以及在法医分析中是重要的。提供方法来分离不含突变的核酸分子，所述核酸分子含有不想要的核苷酸交替的核酸分子群体。

2. 发明申请

WO1998005782A1 TESTIS-SPECIFIC INSULIN HOMOLOG POLYPEPTIDES 审中-公开
标题翻译：特异性胰岛素同源多肽
公开(公告)号：WO1998005782A1
公开(公告)日：1998-02-12
申请号：PCT/US1997013879
申请日：1997-08-01
申请人： ZYMOGENETICS, INC.
发明人： ZYMOGENETICS, INC. , LOK, Si , CONKLIN, Darrell, C. , ADAMS, Robyn, L. , JELMBERG, Anna, C. , LOFTON-DAY, Catherine, E. , JASPERS, Stephen, R.
IPC分类号： C12N15/16
CPC分类号： C07K14/62 , A61K38/00 , C07K14/64
摘要： The present invention provides testis-specific insulin homolog polypeptides and polynucleotides encoding the polypeptides, as well as related compositions and methods are disclosed. The polypeptides and polynucleotides may be used within methods for enhancing viability of cryopreserved sperm, for enhancing sperm motility, to enhance fertilization in methods of assisted reproduction, as contraceptives and other related uses.
摘要翻译：本发明提供了睾丸特异性胰岛素同源物多肽和编码多肽的多核苷酸，以及相关的组合物和方法。多肽和多核苷酸可以用于增强冷冻保存的精子的活力的方法，用于增强精子活力，增强辅助生殖方法中的受精作为避孕药具和其他相关用途。

3. 发明申请

WO1996020955A1 PURIFIED THROMBOPOIETIN AND METHOD OF MAKING IT 审中-公开
标题翻译：纯化色氨酸蛋白酶及其制备方法
公开(公告)号：WO1996020955A1
公开(公告)日：1996-07-11
申请号：PCT/US1995016626
申请日：1995-12-20
申请人： ZYMOGENETICS, INC.
发明人： ZYMOGENETICS, INC. , FORSTROM, John, W. , LOFTON-DAY, Catherine, E. , LOK, Si
IPC分类号： C07K14/52
CPC分类号： C07K14/715 , A61K38/00 , C07K14/52
摘要： Purified mammalian thrombopoietin proteins and methods of making them are disclosed. The proteins are characterized by a Mr=70,000 +/- 10,000 daltons as determined by SDS-polyacrylamide gel electrophoresis under denaturing conditions and are at least 90 % pure with respect to contaminating proteins. The proteins can be prepared by a method in which thrombopoietin is adsorbed to and eluted from a polypeptide comprising a ligand-binding domain of an MPL receptor, then the eluted thrombopoieting is fractionated by anion exchange chromatography.
摘要翻译：公开了纯化的哺乳动物血小板生成蛋白及其制备方法。蛋白质的特征在于Mr = 70,000 +/- 10,000道尔顿，在变性条件下通过SDS-聚丙烯酰胺凝胶电泳测定，并且相对于污染蛋白质至少为90％纯度。蛋白质可以通过其中血小板生成素被吸附到包含MPL受体的配体结合结构域的多肽洗脱的方法来制备，然后通过阴离子交换色谱分离洗脱的血小板。

4. 发明申请

WO1996040904A1 SECRETION LEADER TRAP CLONING METHOD 审中-公开
标题翻译：分析领导者追踪克隆方法
公开(公告)号：WO1996040904A1
公开(公告)日：1996-12-19
申请号：PCT/US1996008936
申请日：1996-06-04
申请人： ZYMOGENETICS, INC.
发明人： ZYMOGENETICS, INC. , LOK, Si
IPC分类号： C12N15/10
CPC分类号： C12N15/1051 , C07K2319/02 , C07K2319/32 , C07K2319/90 , C12N15/625 , C12Q1/6858
摘要： Expression vectors and methods for cloning amino-terminal signal sequences are disclosed. These vectors and methods are useful tools for selecting, expressing and isolating previously unknown signal sequences. In turn, an orphan signal sequence can be used to clone, identify and isolate the corresponding orphan proteins that the orphan signal sequence directs to the surface of a cell. Using these expression vectors and methods, an orphan protein can be cloned in the absence of a functional assay that detects such protein.
摘要翻译：公开了用于克隆氨基末端信号序列的表达载体和方法。这些载体和方法是选择，表达和分离先前未知信号序列的有用工具。反过来，孤儿信号序列可用于克隆，鉴定和分离孤儿信号序列指向细胞表面的相应的孤儿蛋白。使用这些表达载体和方法，可以在不存在检测这种蛋白质的功能测定的情况下克隆孤儿蛋白质。

5. 发明申请

WO1996021025A1 BOVINE FACTOR XIII 审中-公开
标题翻译：牛肉因子十三
公开(公告)号：WO1996021025A1
公开(公告)日：1996-07-11
申请号：PCT/US1995017026
申请日：1995-12-27
申请人： ZYMOGENETICS, INC.
发明人： ZYMOGENETICS, INC. , LOK, Si
IPC分类号： C12N15/54
CPC分类号： C12N9/1044 , A61K38/00
摘要： The present invention provides bovine Factor XIII proteins, DNA molecules and cultured cells expressing bovine Factor XIII. The present invention also provides methods for producing bovine Factor XIII. The invention also includes methods of increasing the water binding capacity of proteins and food products using bovine Factor XIII. Also provided are methods for modifying the amino acid composition of a protein and methods of binding a protein to another insoluble protein using bovine Factor XIII.
摘要翻译：本发明提供牛因子XIII蛋白，DNA分子和表达牛因子XIII的培养细胞。本发明还提供了生产牛因子XIII的方法。本发明还包括使用牛因子XIII增加蛋白质和食品的水结合能力的方法。还提供了用于修饰蛋白质的氨基酸组成的方法和使用牛因子XIII将蛋白质与另一种不溶性蛋白质结合的方法。

6. 发明申请

WO1994005789A1 GLUCAGON RECEPTORS 审中-公开
标题翻译： GLUCAGON受体
公开(公告)号：WO1994005789A1
公开(公告)日：1994-03-17
申请号：PCT/US1993008174
申请日：1993-08-30
申请人： ZYMOGENETICS, INC.
发明人： ZYMOGENETICS, INC. , KINDSVOGLE, Wayne, R. , JELINEK, Laura, J. , SHEPPARD, Paul, O. , GRANT, Francis, J. , KUIJPER, Joseph, L. , FOSTER, Donald, C. , LOK, Si , O'HARA, Patrick, J.
IPC分类号： C12N15/12
CPC分类号： C07K14/723 , A61K38/00 , C07K14/72 , C07K2319/00 , C07K2319/02 , C12Q1/66 , G01N33/74 , G01N2333/605 , G01N2333/72
摘要： The present invention provides isolated DNA molecules comprising a DNA segment encoding a glucagon receptor. Also provided are DNA constructs comprising a first DNA segment encoding a glucagon receptor operably linked to additional DNA segments required for the expression of the first DNA segment, as well as host cells containing such DNA constructs. The present invention also provides amethod for detecting the presence of glucagon antagonists, comprising the steps of (a) exposing a compound in the presence of a glucagon agonist to a recombinant glucagon receptor coupled to a response pathway under conditions and for time sufficient to allow binding of the compound to the receptor and an associated response through the pathway, and (b) detecting a reduction in the stimulation of the response pathway resulting from the binding of the compound to the glucagon receptor, relative to the stimulation of the response pathway by the glucagon agonist alone and therefrom determining the presence of a glucagon antagonist.
摘要翻译：本发明提供了分离的DNA分子，其包含编码胰高血糖素受体的DNA区段。还提供了DNA构建体，其包含编码与第一DNA片段表达所需的另外DNA片段可操作地连接的胰高血糖素受体的第一DNA片段，以及含有该DNA构建体的宿主细胞。本发明还提供了检测胰高血糖素拮抗剂的存在的方法，其包括以下步骤：（a）在有足够的条件和足以允许结合的时间内将化合物在胰高血糖素激动剂存在下暴露于与响应途径偶联的重组胰高血糖素受体化合物与受体的相关反应和通过该途径的相关应答，以及（b）检测由化合物与胰高血糖素受体的结合产生的反应途径的刺激相对于由胰高血糖素激动剂单独和由此决定胰高血糖素拮抗剂的存在。

7. 发明申请

WO1998016635A1 INSULIN HOMOLOGS 审中-公开
标题翻译：胰岛素HOMOLOGS
公开(公告)号：WO1998016635A1
公开(公告)日：1998-04-23
申请号：PCT/US1997018593
申请日：1997-10-15
申请人： ZYMOGENETICS, INC.
发明人： ZYMOGENETICS, INC. , CONKLIN, Darrell, C. , LOFTON-DAY, Catherine, E. , LOK, Si , JASPERS, Stephen, R.
IPC分类号： C12N15/00
CPC分类号： C07K14/64 , A01K2217/05
摘要： The present invention relates to polynucleotide and polypeptide molecules for a novel insulin homolog. The polypeptides, and polynucleotides encoding them, have located to chromosome 12. The present invention also includes antibodies to the insulin homolog polypeptides.
摘要翻译：本发明涉及用于新型胰岛素同系物的多核苷酸和多肽分子。多肽和编码它们的多核苷酸已经位于染色体12上。本发明还包括对胰岛素同源多肽的抗体。

8. 发明申请

WO1996017062A1 LOW MOLECULAR WEIGHT THROMBOPOIETIN 审中-公开
标题翻译：低分子重量血小板计数
公开(公告)号：WO1996017062A1
公开(公告)日：1996-06-06
申请号：PCT/US1995014929
申请日：1995-11-15
申请人： ZYMOGENETICS, INC.
发明人： ZYMOGENETICS, INC. , BUDDLE, Michele, M. , DOWNEY, William , FOSTER, Donald, C. , FORSTROM, John, W. , LOK, Si , OSBORN, Sherri, G.
IPC分类号： C12N15/19
CPC分类号： C07K14/524 , A01K2217/05 , A61K38/00
摘要： Isolated mammalian thrombopoietins are disclosed. The thrombopoietins include polypeptides characterized by an amino acid sequence selected from the group consisting of (a) a sequence of amino acids as shown in SEQ ID NO:2 having an amino terminus at Ser(45) and a carboxyl terminus between Ser(208) and Asn(216), inclusive; a sequence of amino acids as shown in SEQ ID NO:4 having an amino terminus at Ser(22) and a carboxyl terminus between Arg(185) and Asn(193), inclusive; a sequence of amino acids as shown in SEQ ID NO:4 having an amino terminus at Ser(22) and a carboxyl terminus at Arg(198); a sequence of amino acids as shown in SEQ ID NO:4 having an amino terminus at Ser(22) and a carboxyl terminus at Phe(207); and allelic variants of (a)-(d). Also disclosed are isolated DNA molecules encoding these polypeptides as well as vectors, cells and methods that can be used for the production of the polypeptides.
摘要翻译：公开了分离的哺乳动物血小板生成素。血小板生成素包括以氨基酸序列为特征的多肽，所述氨基酸序列选自（a）SEQ ID NO：2所示氨基酸序列，其具有Ser（45）的氨基末端和Ser（208）之间的羧基末端，和Asn（216）（含）; 如SEQ ID NO：4所示的氨基酸序列，其具有Ser（22）的氨基末端和Arg（185）和Asn（193）之间的羧基末端（包括端值）; 如SEQ ID NO：4所示的氨基酸序列，其在Ser（22）具有氨基末端，在Arg处具有羧基末端（198）; 如SEQ ID NO：4所示的氨基酸序列，其在Ser（22）具有氨基末端，在Phe（207）具有羧基末端; 和（a） - （d）的等位变体。还公开了编码这些多肽的分离的DNA分子以及可用于产生多肽的载体，细胞和方法。

9. 发明申请

WO1995021920A1 HEMATOPOIETIC PROTEIN AND MATERIALS AND METHODS FOR MAKING IT 审中-公开
标题翻译： HEMATOPOIETIC蛋白质及其制备方法
公开(公告)号：WO1995021920A1
公开(公告)日：1995-08-17
申请号：PCT/US1994008806
申请日：1994-08-05
申请人： ZYMOGENETICS, INC. , UNIVERSITY OF WASHINGTON
发明人： ZYMOGENETICS, INC. , UNIVERSITY OF WASHINGTON , HOLLY, Richard, D. , LOK, Si , FOSTER, Donald, C. , HAGEN, Frederick, S. , KAUSHANSKY, Kenneth , KUIJPER, Joseph, L. , LOFTON-DAY, Catherine , OORT, Pieter, J. , BURKHEAD, Steven, K.
IPC分类号： C12N15/19
CPC分类号： G01N33/5055 , A61K38/196 , C07K14/705 , C07K14/715 , C12N5/0642 , C12N2501/145 , C12N2501/22 , G01N33/5005 , G01N33/5008 , G01N33/5088 , G01N33/5094
摘要： Hematopoietic proteins and polypeptide fragments thereof are provided, including proteins and polypeptides from mice and humans. Also provided are DNA molecules encoding the proteins and polypeptides, as well as vectors and cells useful in their production. Antibodies that bind to an epitope on the proteins are also provided. The proteins and polypeptides are useful for in vivo and ex vivo therapy, and as reagents for cell culture and investigation of cell proliferation and development.
摘要翻译：提供了造血蛋白及其多肽片段，包括来自小鼠和人的蛋白质和多肽。还提供了编码蛋白质和多肽的DNA分子，以及可用于其生产的载体和细胞。还提供了与蛋白质上的表位结合的抗体。蛋白质和多肽可用于体内和离体治疗，以及作为细胞培养和细胞增殖和发育调查的试剂。

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