专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2003087839A1 METHOD FOR CHARACTERISING ANALYTES 审中-公开
标题翻译：表征分析的方法
公开(公告)号：WO2003087839A1
公开(公告)日：2003-10-23
申请号：PCT/GB2003/001485
申请日：2003-04-04
申请人： XZILLION GMBH & CO. KG , THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , MEYER, Markus , JUERGEN, Schafer , NEUMANN, Thomas
发明人： THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , MEYER, Markus , JUERGEN, Schafer , NEUMANN, Thomas
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , C07K1/13 , G01N33/6851
摘要： Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterise the analyte.
摘要翻译：提供了一种通过基质辅助激光解吸电离（MALDI）质谱鉴定分析物的方法，该方法包括：（a）用预吸收光的光吸收标记物以预定的频率标记分析物，形成标记的分析物; （b）将标记的分析物包埋在由吸收光的至少一种化合物形成的基质中，以形成嵌入的标记分析物; （c）通过将嵌入的标记分析物暴露于具有预定频率的光来解吸，形成解吸附的分析物; 和（d）通过质谱检测解吸附的分析物来表征分析物。

2. 发明申请

WO2003025576A2 MASS LABELS 审中-公开
标题翻译：质量标签
公开(公告)号：WO2003025576A2
公开(公告)日：2003-03-27
申请号：PCT/GB2002/004240
申请日：2002-09-16
申请人： XZILLION GMBH & CO. KG , THOMPSON, Andrew, Hugin , HAMON, Christian , SCHAFER, Jurgen , KUHN, Karsten , SCHWARZ, Josef , NEUMANN, Thomas
发明人： THOMPSON, Andrew, Hugin , HAMON, Christian , SCHAFER, Jurgen , KUHN, Karsten , SCHWARZ, Josef , NEUMANN, Thomas
IPC分类号： G01N33/58
CPC分类号： G01N33/6848 , G01N33/68 , G01N33/6851 , G01N2458/15 , H01J49/04
摘要： Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker having at least one amide bond to a mass normalisation moiety, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectromety, and wherein the mass marker moiety comprises an amino acid and the mass normalisation moiety comprises an amino acid.
摘要翻译：提供了一组两个或更多个质量标签，该组中的每个标签包含经由具有至少一个酰胺键的质量标准化部分连接到质量标准化部分的质量标记部分，其中该组中每个标签的总质量可以为相同或不同，组中每个标记物的质量标记物部分的质量可以相同或不同，并且其中在具有共同质量的质量标记物部分的组中的任何标签组中，每个标签具有总体质量不同于该组中的所有其它标记，并且其中在组中具有共同聚集体的任何标签组中，每个标记具有与该组中所有其他质量标记部分的质量不同的质量标记部分，使得全部组中的质量标记物通过质谱可以彼此区分，并且其中质量标记物部分包含氨基酸，质量标准化部分包含氨基酸。

3. 发明申请

WO2002099124A3 CHARACTERISING POLYPEPTIDES 审中-公开
公开(公告)号：WO2002099124A3
公开(公告)日：2002-12-12
申请号：PCT/GB2002/002601
申请日：2002-06-07
申请人： XZILLION GMBH & CO. KG , HAMON, Christian , THOMPSON, Andrew , KUHN, Karsten , NEUMANN, Thomas , JOUBERT, Richard , JOHNSTONE, Robert , SCHMIDT, Gunter
发明人： HAMON, Christian , THOMPSON, Andrew , KUHN, Karsten , NEUMANN, Thomas , JOUBERT, Richard , JOHNSTONE, Robert , SCHMIDT, Gunter
IPC分类号： C12Q1/37
摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.

4. 发明申请

WO2002099124A2 CHARACTERISING POLYPEPTIDES 审中-公开
标题翻译：表征多糖
公开(公告)号：WO2002099124A2
公开(公告)日：2002-12-12
申请号：PCT/GB2002/002601
申请日：2002-06-07
申请人： XZILLION GMBH & CO. KG , HAMON, Christian , THOMPSON, Andrew , KUHN, Karsten , NEUMANN, Thomas , JOUBERT, Richard , JOHNSTONE, Robert , SCHMIDT, Gunter
发明人： HAMON, Christian , THOMPSON, Andrew , KUHN, Karsten , NEUMANN, Thomas , JOUBERT, Richard , JOHNSTONE, Robert , SCHMIDT, Gunter
IPC分类号： C12Q1/37
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.
摘要翻译：提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）将包含一种或多种多肽的样品与赖氨酸反应剂接触以将ε-氨基封端; （b）任选地使多肽样品与胺反应性试剂反应以阻断α-氨基; （c）用裂解试剂消化多肽样品以产生肽片段; （d）任选地使切割试剂失活; （e）去除具有未封端或未封闭氨基的那些肽; 和（f）回收N-末端肽。

5. 发明申请

WO2002099435A1 METHOD FOR CHARACTERIZING POLYPEPTIDES 审中-公开
标题翻译：表征多糖的方法
公开(公告)号：WO2002099435A1
公开(公告)日：2002-12-12
申请号：PCT/GB2002/002605
申请日：2002-06-07
申请人： XZILLION GMBH & CO. KG , HAMON, Christian , THOMPSON, Andrew , NEUMANN, Thomas , JOHNSTONE, Robert , MOHAMMED, Abdul Karim Abed
发明人： HAMON, Christian , THOMPSON, Andrew , NEUMANN, Thomas , JOHNSTONE, Robert , MOHAMMED, Abdul Karim Abed
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide, which method comprises the steps of: (a) optionnally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analysing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.
摘要翻译：提供了表征多肽的方法，该方法包括以下步骤：（a）选择性地减少多肽中的半胱氨酸二硫桥，以形成游离硫醇，并封端游离硫醇; （b）用序列特异性切割试剂切割多肽以形成肽片段; （c）任选地使切割试剂失活; （d）封端与赖氨酸反应剂一起存在的一个或多个ε-氨基; （e）通过质谱分析肽片段以形成多肽的质量指纹图谱; 和（f）从质量指纹图谱确定多肽的身份。

6. 发明申请

WO2002099436A2 CHARACTERISING POLYPEPTIDES 审中-公开
标题翻译：表征多糖
公开(公告)号：WO2002099436A2
公开(公告)日：2002-12-12
申请号：PCT/GB2002/002778
申请日：2002-06-07
申请人： XZILLION GMBH & CO. KG , THOMPSON, Andrew , HAMON, Christian , NEUMANN, Thomas , PICKERING-BROWN, Stuart
发明人： THOMPSON, Andrew , HAMON, Christian , NEUMANN, Thomas , PICKERING-BROWN, Stuart
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , C07K1/12 , C07K1/128 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of : (a) optionally reducing disulphide linkages in the polypeptides, if they are present and capping free thiols in the polypeptides, if they are present; (b) contacting a sample comprising one or more polypeptides with a cleavage reagent which cleaves one or more polypeptides on the C-terminal side of a lysine residue to produce peptide fragments; (c) optionally deactivating the cleavage reagent; (d) contacting the sample with a lysine reactive agent to cap ε-amino groups; (e) removing those peptides having capped ε-amino groups; and (f) recovering the C-terminal peptides.
摘要翻译：提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）如果它们存在，任选地还原多肽中的二硫键，如果它们存在则任选地还原多肽中的无帽硫醇; （b）使包含一种或多种多肽的样品与裂解试剂接触，所述切割试剂在赖氨酸残基的C末端侧切割一个或多个多肽以产生肽片段; （c）任选地使切割试剂失活; （d）使样品与赖氨酸反应剂接触以将ε-氨基封端; （e）除去具有封端的ε-氨基的那些肽; 和（f）回收C末端肽。

7. 发明申请

WO2003021274A3 VERFAHREN ZUR AUFTRENNUNG VON REVERSIBEL CHEMISCH MODIFIZIERTEN MAKROMOLEKÜLEN 审中-公开
公开(公告)号：WO2003021274A3
公开(公告)日：2003-03-13
申请号：PCT/EP2002/009808
申请日：2002-09-03
申请人： XZILLION GMBH & CO KG , TE KAAT, Kai, Hendrik , HAMON, Christian , JOUBERT, Richard , NEUMANN, Thomas , SCHWIER, Wolfgang
发明人： TE KAAT, Kai, Hendrik , HAMON, Christian , JOUBERT, Richard , NEUMANN, Thomas , SCHWIER, Wolfgang
IPC分类号： G01N33/68
摘要： Die vorliegende Erfindung betrifft ein Verfahren zur Auftrennung einer Probe von Makromolekülen, insbesondere zur elektrophoretischen Auftrennung von Proteinen, dadurch gekennzeichnet, dass mindestens ein Makromolekül aus der Probe vor Durchführung des Trennverfahrens durch mindestens eine teilweise oder vollständig unter milden Bedingungen wieder abspaltbare Gruppe modifiziert wird.

8. 发明申请

WO2003021274A2 VERFAHREN ZUR AUFTRENNUNG VON REVERSIBEL CHEMISCH MODIFIZIERTEN MAKROMOLEKÜLEN 审中-公开
标题翻译：分离可逆化学修饰的大分子的方法
公开(公告)号：WO2003021274A2
公开(公告)日：2003-03-13
申请号：PCT/EP2002/009808
申请日：2002-09-03
申请人： XZILLION GMBH & CO KG , TE KAAT, Kai, Hendrik , HAMON, Christian , JOUBERT, Richard , NEUMANN, Thomas , SCHWIER, Wolfgang
发明人： TE KAAT, Kai, Hendrik , HAMON, Christian , JOUBERT, Richard , NEUMANN, Thomas , SCHWIER, Wolfgang
IPC分类号： G01N33/68
CPC分类号： G01N27/44726 , C07K1/006 , C07K1/26 , C07K1/28
摘要： Die vorliegende Erfindung betrifft ein Verfahren zur Auftrennung einer Probe von Makromolekülen, insbesondere zur elektrophoretischen Auftrennung von Proteinen, dadurch gekennzeichnet, dass mindestens ein Makromolekül aus der Probe vor Durchführung des Trennverfahrens durch mindestens eine teilweise oder vollständig unter milden Bedingungen wieder abspaltbare Gruppe modifiziert wird.
摘要翻译：
本发明涉及一种用于大分子导航使用LEN的样品的分离的方法，特别是用于蛋白质的电泳分离，通过至少进行分离过程的导航用途引导之前其特征在于，至少一个大分子导航用途升从样品在温和条件下部分或完全修改的组被修改。

9. 发明申请

WO2012020080A3 LIGANDS FOR AFFINITY CHROMATOGRAPHY 审中-公开
公开(公告)号：WO2012020080A3
公开(公告)日：2012-02-16
申请号：PCT/EP2011/063831
申请日：2011-08-11
申请人： GRAFFINITY PHARMACEUTICALS GMBH , BITTERMANN, Holger , BURKERT, Klaus , ARNOLD, Marc , KEIL, Oliver , NEUMANN, Thomas , OTT, Inge , SCHMIDT, Kristina , SCHWIZER, Daniel , SEKUL, Renate
发明人： BITTERMANN, Holger , BURKERT, Klaus , ARNOLD, Marc , KEIL, Oliver , NEUMANN, Thomas , OTT, Inge , SCHMIDT, Kristina , SCHWIZER, Daniel , SEKUL, Renate
IPC分类号： C07K1/22 , B01D15/38 , B01J20/286 , B01J20/32
摘要： The present invention relates to the use of a ligand-substituted matrix comprising a support material and at least one ligand covalently bonded to the support material, the being represented by formula (I) wherein L is the linking point on the support material to which the ligand is attached; V is a spacer group; v is 0 or 1; R 1 is hydrogen or C 1 to C 4 alkyl, preferably hydrogen or methyl; and more preferably hydrogen; R 2 is hydrogen or C 1 to C 4 alkyl, preferably hydrogen; Ar 1 is a divalent 5- or 6-membered substituted or unsubstituted aromatic ring; and Ar 2 is 5- or 6-membered aromatic ring which may be unsubstituted or which is (a) attached to a further 5- or 6-membered aromatic ring via a single bond; or (b) fused to a further 5- or 6-membered aromatic or non aromatic ring, as part of a multicyclic ring system; or (c) attached to at least one substituent selected from C 1 to C 4 alkyl; C 2 to C 4 alkenyl; C 2 to C 4 alkynyl; a halogen; C 1 to C 4 haloalkyl; hydroxyl-substituted C 1 to C 4 alkyl; C 1 to C 4 alkoxy; hydroxyl-substituted C 1 to C 4 alkoxy; C 1 to C 4 alkylamino; C 1 to C 4 alkylthio; and combinations thereof, for affinity purification of a protein.

10. 发明申请

WO2012017021A2 LIGANDS FOR ANTIBODY AND Fc-FUSION PROTEIN PURIFICATION BY AFFINITY CHROMATOGRAPHY 审中-公开
标题翻译：亲和层析技术用于抗体和Fc融合蛋白的亲和层析纯化
公开(公告)号：WO2012017021A2
公开(公告)日：2012-02-09
申请号：PCT/EP2011/063392
申请日：2011-08-03
申请人： GRAFFINITY PHARMACEUTICALS GMBH , BITTERMANN, Holger , BURKERT, Klaus , ARNOLD, Marc , KEIL, Oliver , NEUMANN, Thomas , OTT, Inge , SCHMIDT, Kristina , SCHWIZER, Daniel , SEKUL, Renate
发明人： BITTERMANN, Holger , BURKERT, Klaus , ARNOLD, Marc , KEIL, Oliver , NEUMANN, Thomas , OTT, Inge , SCHMIDT, Kristina , SCHWIZER, Daniel , SEKUL, Renate
IPC分类号： C07K1/22
CPC分类号： C07K1/22 , B01D15/3804 , B01J20/289 , B01J20/3251 , B01J20/3253 , B01J20/3255 , C07D209/42 , C07D213/82 , C07D217/26 , C07D231/14 , C07D231/56 , C07D235/24 , C07D261/18 , C07D261/20 , C07D271/06 , C07D277/46 , C07D277/56 , C07D277/68 , C07D277/82 , C07D307/87 , C07D403/04 , C07D405/04 , C07D409/04 , C07D413/04 , C07D417/04 , C07D417/14 , C07D471/04 , C07D487/04 , C07D513/04 , C07D519/00 , C07K16/00 , C08B37/0039
摘要： The present invention relates to the use for affinity purification of an antibody or an fragment of an antibody, of a ligand-substituted matrix comprising a support material and at least one ligand covalently bonded to the support material, the ligand being represented by formula (I) wherein Sp is a spacer group; v is 0 or 1; Am is an amide group -NR 1 -C(O)-, and wherein either NR 1 is attached to Ar 1 and C(O)- is attached to Ar 2 , or -C(O)- is attached to Ar 1 and NR 1 is attached to Ar 2 ; and R 1 is hydrogen or C 1 to C 4 alkyl, preferably hydrogen or methyl; and more preferably hydrogen; Ar 1 is a divalent 5- or 6-membered substituted or unsubstituted aromatic ring; Ar 2 is 5- or 6-membered heterocyclic aromatic ring which is (a) attached to a further 5- or 6-membered aromatic ring via a single bond; or (b) fused to a further 5- or 6-membered aromatic ring as part of a multicyclic ring system; or (c) attached to at least one substituent selected from C 1 to C 4 alkyl; C 2 to C 4 alkenyl; C 2 to C 4 alkynyl; a halogen; C 1 to C 4 haloalkyl; hydroxyl-substituted C 1 to C 4 alkyl; C 1 to C 4 alkoxy; hydroxyl-substituted C 1 to C 4 alkoxy; C 1 to C 4 alkylamino; C 1 to C 4 alkylthio; and combinations thereof.
摘要翻译：本发明涉及亲和纯化抗体或抗体片段，包含载体材料和至少一种共价结合于载体材料的配体的配体取代基质的用途，该配体由式（I）表示，其中Sp是间隔基团; v是0或1; Am是酰胺基-NR 1 -C（O） - ，其中或者NR 1与Ar 1和C（O）连接，） - 连接到Ar 2上，或者-C（O） - 连接到Ar 1上并且NR 1连接到Ar 2 ; 和R 1是氢或C 1至C 4烷基，优选氢或甲基; 更优选氢; Ar 1是二价5-或6-元取代或未取代的芳族环; Ar 2是5或6元杂环芳环，其（a）通过单键与另外的5或6元芳环连接; 或（b）作为多环体系的一部分与另一个5或6元芳族环稠合; 或（c）连接至少一个选自C 1至C 4烷基的取代基; C 2至C 4链烯基; C≡C2至C≡4炔基; 卤素; C 1至C 4卤代烷基; 羟基取代的C 1至C 4烷基; C 1〜C 4烷氧基; C 1〜C 4烷氧基; C 1〜C 4烷氧基; 羟基取代的C 1至C 4烷氧基; C 1至C 4烷基氨基; C 1至C 4烷硫基; 和它们的组合。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式