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1. 发明申请

WO2004086050A3 LABELLING AGENTS FOR MASS SPECTROMETRY COMPRISING TERTIARY AMINES 审中-公开
公开(公告)号：WO2004086050A3
公开(公告)日：2004-10-07
申请号：PCT/GB2004/001167
申请日：2004-03-18
申请人： XZILLION GMBH & CO. KG , PROTEOME SCIENCES PLC , HAMON, Christian , KUHN, Karsten , THOMPSON, Andrew , REUSCHLING, Dieter , SCHAEFER, Juergen
发明人： HAMON, Christian , KUHN, Karsten , THOMPSON, Andrew , REUSCHLING, Dieter , SCHAEFER, Juergen
IPC分类号： G01N33/58
摘要： Provided is a method for characterising a molecule by mass spectrometry, which molecule comprises one or more free amino groups, which method comprises: (a) reacting one or more free amino groups in the molecule with a mass tag reagent comprising a reactive functionality capable of reacting with an amino group, and a tertiary amino group linked to the reactive functionality; and (b) characterising the molecule by mass spectrometry.

2. 发明申请

WO2003087839A1 METHOD FOR CHARACTERISING ANALYTES 审中-公开
标题翻译：表征分析的方法
公开(公告)号：WO2003087839A1
公开(公告)日：2003-10-23
申请号：PCT/GB2003/001485
申请日：2003-04-04
申请人： XZILLION GMBH & CO. KG , THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , MEYER, Markus , JUERGEN, Schafer , NEUMANN, Thomas
发明人： THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , MEYER, Markus , JUERGEN, Schafer , NEUMANN, Thomas
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , C07K1/13 , G01N33/6851
摘要： Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterise the analyte.
摘要翻译：提供了一种通过基质辅助激光解吸电离（MALDI）质谱鉴定分析物的方法，该方法包括：（a）用预吸收光的光吸收标记物以预定的频率标记分析物，形成标记的分析物; （b）将标记的分析物包埋在由吸收光的至少一种化合物形成的基质中，以形成嵌入的标记分析物; （c）通过将嵌入的标记分析物暴露于具有预定频率的光来解吸，形成解吸附的分析物; 和（d）通过质谱检测解吸附的分析物来表征分析物。

3. 发明申请

WO2002099435A1 METHOD FOR CHARACTERIZING POLYPEPTIDES 审中-公开
标题翻译：表征多糖的方法
公开(公告)号：WO2002099435A1
公开(公告)日：2002-12-12
申请号：PCT/GB2002/002605
申请日：2002-06-07
申请人： XZILLION GMBH & CO. KG , HAMON, Christian , THOMPSON, Andrew , NEUMANN, Thomas , JOHNSTONE, Robert , MOHAMMED, Abdul Karim Abed
发明人： HAMON, Christian , THOMPSON, Andrew , NEUMANN, Thomas , JOHNSTONE, Robert , MOHAMMED, Abdul Karim Abed
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide, which method comprises the steps of: (a) optionnally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analysing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.
摘要翻译：提供了表征多肽的方法，该方法包括以下步骤：（a）选择性地减少多肽中的半胱氨酸二硫桥，以形成游离硫醇，并封端游离硫醇; （b）用序列特异性切割试剂切割多肽以形成肽片段; （c）任选地使切割试剂失活; （d）封端与赖氨酸反应剂一起存在的一个或多个ε-氨基; （e）通过质谱分析肽片段以形成多肽的质量指纹图谱; 和（f）从质量指纹图谱确定多肽的身份。

4. 发明申请

WO2004102180A2 MASS SPECTROMETRY 审中-公开
标题翻译：质谱
公开(公告)号：WO2004102180A2
公开(公告)日：2004-11-25
申请号：PCT/GB2004/002059
申请日：2004-05-13
申请人： XZILLION GMBH & CO. KG , PROTEOME SCIENCES PLC , BAUER, Ute , MORAGA-MARTINEZ, Roger, Alfonso , SCHWARZ, Josef
发明人： BAUER, Ute , MORAGA-MARTINEZ, Roger, Alfonso , SCHWARZ, Josef
IPC分类号： G01N30/00
CPC分类号： H01J49/0036
摘要： Provided is a method for processing data from a mass spectrum generated from a sample, which method comprises: (a) selecting a first peak in the mass spectrum; (b) selecting a first monoisotopic reference ion having a first charge state, which first reference ion could contribute to the first peak; (c) for one or more other isotopic forms of the first reference ion determining one or more further expected peaks in the mass spectrum; (d) comparing one or more of the determined further expected peaks with the mass spectrum to determine whether there are one or more peaks present in the spectrum that match the one or more determined further expected peaks; (e) if one or more of the determined further expected peaks match one or more of the peaks in the mass spectrum, designating the first peak as a data peak, and optionally designating the one or more peaks present in the spectrum that match the one or more determined further expected peaks as data peaks; (f) if the determined further expected peaks do not match peaks in the mass spectrum, repeating steps (b) to (e) with one or more further reference ions in one or more further charge states; (g) optionally if the first peak cannot be designated as a data peak for a reference ion in the first charge state, or for a further reference ion in the further charge states, designating the first peak as a non-data peak; (h) optionally repeating steps (a) - (g) for one or more further peaks in the mass spectrum.
摘要翻译：提供一种从样品生成的质谱中处理数据的方法，该方法包括：（a）选择质谱中的第一个峰; （b）选择具有第一电荷状态的第一单同位素参考离子，该第一参考离子可有助于第一峰; （c）对于确定质谱中一个或多个其它预期峰的第一参考离子的一种或多种其它同位素形式; （d）将确定的进一步预期峰中的一个或多个与质谱进行比较，以确定谱中是否存在与一个或多个确定的进一步预期峰相匹配的一个或多个峰; （e）如果确定的进一步预期的峰中的一个或多个与质谱中的一个或多个峰匹配，则将第一峰指定为数据峰，并且可选地指定存在于频谱中的与该一个相匹配的一个或多个峰或更多确定的进一步预期峰值作为数据峰; （f）如果确定的进一步预期的峰与质谱中的峰不匹配，则在一个或多个另外的电荷状态下用一个或多个另外的参考离子重复步骤（b）至（e）（g）可选地，如果第一峰不能被指定为在第一充电状态中的参考离子的数据峰，或者在另外的充电状态中指定另外的参考离子，则将第一峰指定为非数据峰; （h）任选地重复步骤（a） - （g）在质谱中的一个或多个另外的峰。

5. 发明申请

WO2003104813A3 MODIFICATION OF POLYPEPTIDES FOR CHARACTERISATION PURPOSES 审中-公开
公开(公告)号：WO2003104813A3
公开(公告)日：2003-12-18
申请号：PCT/GB2003/002451
申请日：2003-06-06
申请人： XZILLION GMBH & CO. KG , PROTEOME SCIENCES PLC , THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , BAUER, Ute , MEUMANN, Thomas
发明人： THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , BAUER, Ute , MEUMANN, Thomas
IPC分类号： C07K143/00
摘要： Provided is a method of solubilising a polypeptide, which polypeptide is insoluble or sparingly soluble in an aqueous medium, which method comprises either: (a) contacting the polypeptide with a sequence specific cleavage agent in a non-aqueous medium, to produce a product which is soluble in an aqueous medium; or (b) contacting the polypeptide with a dicarboxylic anhydride to modify one or more amino groups in the polypeptide, to produce a product which is soluble in an aqueous medium. Also provided is a relational database comprising: (a) a table of parent polypeptide sequences; and (b) a table of peptides, which peptides are expected products of putative reactions of polypeptides from the table of polypeptides; wherein each of the parent polypeptide sequences is relatable by the database to one or more peptides from the table of peptides.

6. 发明申请

WO2002099124A2 CHARACTERISING POLYPEPTIDES 审中-公开
标题翻译：表征多糖
公开(公告)号：WO2002099124A2
公开(公告)日：2002-12-12
申请号：PCT/GB2002/002601
申请日：2002-06-07
申请人： XZILLION GMBH & CO. KG , HAMON, Christian , THOMPSON, Andrew , KUHN, Karsten , NEUMANN, Thomas , JOUBERT, Richard , JOHNSTONE, Robert , SCHMIDT, Gunter
发明人： HAMON, Christian , THOMPSON, Andrew , KUHN, Karsten , NEUMANN, Thomas , JOUBERT, Richard , JOHNSTONE, Robert , SCHMIDT, Gunter
IPC分类号： C12Q1/37
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.
摘要翻译：提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）将包含一种或多种多肽的样品与赖氨酸反应剂接触以将ε-氨基封端; （b）任选地使多肽样品与胺反应性试剂反应以阻断α-氨基; （c）用裂解试剂消化多肽样品以产生肽片段; （d）任选地使切割试剂失活; （e）去除具有未封端或未封闭氨基的那些肽; 和（f）回收N-末端肽。

7. 发明申请

WO2005012914A2 CHARACTERISING POLYPEPTIDES 审中-公开
标题翻译：表征多糖
公开(公告)号：WO2005012914A2
公开(公告)日：2005-02-10
申请号：PCT/GB2004/003139
申请日：2004-07-22
申请人： XZILLION GMBH & CO. KG , PROTEOME SCIENCES PLC , SCHAFER, Jurgen , HAMON, Christian , SCHWARZ, Josef , PEARCE, Christopher
发明人： SCHAFER, Jurgen , HAMON, Christian , SCHWARZ, Josef , PEARCE, Christopher
IPC分类号： G01N33/68
CPC分类号： G01N33/6851 , C07D403/14 , C07D413/14 , C07K1/13 , G01N33/533 , G01N33/6848
摘要： Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterise the analyte; wherein the light absorbing label comprises a fluorophore moiety, and wherein prior to detecting by mass spectrometry, the analyte is selected for detection on the basis of its fluorophore moiety.
摘要翻译：提供了一种用于通过基质辅助激光解吸电离（MALDI）质谱法来表征分析物的方法，该方法包括：（a）用吸收预定频率的光的吸光标签标记分析物，以形成标记分析物; （b）将标记的分析物包埋在由吸收光的至少一种化合物形成的基质中，形成嵌入的标记分析物; （c）通过将嵌入的标记分析物暴露于具有预定频率的光来解吸，形成解吸附的分析物; 和（d）通过质谱检测解吸附的分析物来表征分析物; 其中所述光吸收标签包含荧光团部分，并且其中在通过质谱法检测之前，选择所述分析物用于基于其荧光团部分进行检测。

8. 发明申请

WO2003104813A2 CHARACTERISING POLYPEPTIDES 审中-公开
标题翻译：表征多糖
公开(公告)号：WO2003104813A2
公开(公告)日：2003-12-18
申请号：PCT/GB2003/002451
申请日：2003-06-06
申请人： XZILLION GMBH & CO. KG , PROTEOME SCIENCES PLC , THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , BAUER, Ute , MEUMANN, Thomas
发明人： THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , BAUER, Ute , MEUMANN, Thomas
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/6803 , G01N33/6818
摘要： Provided is a method of solubilising a polypeptide, which polypeptide is insoluble or sparingly soluble in an aqueous medium, which method comprises either: (a) contacting the polypeptide with a sequence specific cleavage agent in a non-aqueous medium, to produce a product which is soluble in an aqueous medium; or (b) contacting the polypeptide with a dicarboxylic anhydride to modify one or more amino groups in the polypeptide, to produce a product which is soluble in an aqueous medium. Also provided is a relational database comprising: (a) a table of parent polypeptide sequences; and (b) a table of peptides, which peptides are expected products of putative reactions of polypeptides from the table of polypeptides; wherein each of the parent polypeptide sequences is relatable by the database to one or more peptides from the table of peptides.
摘要翻译：提供了一种增溶多肽的方法，该多肽在水性介质中不溶或微溶，该方法包括：（a）使多肽与非水介质中的序列特异性切割剂接触，以产生产物，可溶于水介质; 或（b）使多肽与二羧酸酐接触以改变多肽中的一个或多个氨基，以产生可溶于水性介质的产物。还提供了一种关系数据库，其包括：（a）亲本多肽序列表; 和（b）肽表，该肽是来自多肽表的多肽的推定反应的预期产物; 其中每个母体多肽序列可以通过数据库与肽表中的一种或多种肽相关。

9. 发明申请

WO2003025576A2 MASS LABELS 审中-公开
标题翻译：质量标签
公开(公告)号：WO2003025576A2
公开(公告)日：2003-03-27
申请号：PCT/GB2002/004240
申请日：2002-09-16
申请人： XZILLION GMBH & CO. KG , THOMPSON, Andrew, Hugin , HAMON, Christian , SCHAFER, Jurgen , KUHN, Karsten , SCHWARZ, Josef , NEUMANN, Thomas
发明人： THOMPSON, Andrew, Hugin , HAMON, Christian , SCHAFER, Jurgen , KUHN, Karsten , SCHWARZ, Josef , NEUMANN, Thomas
IPC分类号： G01N33/58
CPC分类号： G01N33/6848 , G01N33/68 , G01N33/6851 , G01N2458/15 , H01J49/04
摘要： Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker having at least one amide bond to a mass normalisation moiety, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectromety, and wherein the mass marker moiety comprises an amino acid and the mass normalisation moiety comprises an amino acid.
摘要翻译：提供了一组两个或更多个质量标签，该组中的每个标签包含经由具有至少一个酰胺键的质量标准化部分连接到质量标准化部分的质量标记部分，其中该组中每个标签的总质量可以为相同或不同，组中每个标记物的质量标记物部分的质量可以相同或不同，并且其中在具有共同质量的质量标记物部分的组中的任何标签组中，每个标签具有总体质量不同于该组中的所有其它标记，并且其中在组中具有共同聚集体的任何标签组中，每个标记具有与该组中所有其他质量标记部分的质量不同的质量标记部分，使得全部组中的质量标记物通过质谱可以彼此区分，并且其中质量标记物部分包含氨基酸，质量标准化部分包含氨基酸。

10. 发明申请

WO2002099436A2 CHARACTERISING POLYPEPTIDES 审中-公开
标题翻译：表征多糖
公开(公告)号：WO2002099436A2
公开(公告)日：2002-12-12
申请号：PCT/GB2002/002778
申请日：2002-06-07
申请人： XZILLION GMBH & CO. KG , THOMPSON, Andrew , HAMON, Christian , NEUMANN, Thomas , PICKERING-BROWN, Stuart
发明人： THOMPSON, Andrew , HAMON, Christian , NEUMANN, Thomas , PICKERING-BROWN, Stuart
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , C07K1/12 , C07K1/128 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of : (a) optionally reducing disulphide linkages in the polypeptides, if they are present and capping free thiols in the polypeptides, if they are present; (b) contacting a sample comprising one or more polypeptides with a cleavage reagent which cleaves one or more polypeptides on the C-terminal side of a lysine residue to produce peptide fragments; (c) optionally deactivating the cleavage reagent; (d) contacting the sample with a lysine reactive agent to cap ε-amino groups; (e) removing those peptides having capped ε-amino groups; and (f) recovering the C-terminal peptides.
摘要翻译：提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）如果它们存在，任选地还原多肽中的二硫键，如果它们存在则任选地还原多肽中的无帽硫醇; （b）使包含一种或多种多肽的样品与裂解试剂接触，所述切割试剂在赖氨酸残基的C末端侧切割一个或多个多肽以产生肽片段; （c）任选地使切割试剂失活; （d）使样品与赖氨酸反应剂接触以将ε-氨基封端; （e）除去具有封端的ε-氨基的那些肽; 和（f）回收C末端肽。

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