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1. 发明申请

WO2005100387A1 MONOMERIC RED FLUORESCENT PROTEINS 审中-公开
标题翻译：单体红色荧光蛋白
公开(公告)号：WO2005100387A1
公开(公告)日：2005-10-27
申请号：PCT/US2005/011753
申请日：2005-04-07
申请人： THE UNIVERSITY OF CHICAGO , THE UNIVERSITY OF WASHINGTON , GLICK, Benjamin, S. , BEVIS, Brooke , STRONGIN, Daniel, E. , BAKER, David , SCALLEY-KIM, Michelle
发明人： GLICK, Benjamin, S. , BEVIS, Brooke , STRONGIN, Daniel, E. , BAKER, David , SCALLEY-KIM, Michelle
IPC分类号： C07K14/435
CPC分类号： C07K14/43595 , C07K14/43504 , Y10S530/802
摘要： Disclosed are sequences encoding monomeric variants of DsRed fluorescent proteins and methods of use.
摘要翻译：公开了编码DsRed荧光蛋白的单体变体的序列和使用方法。

2. 发明申请

WO2003054158A3 RAPIDLY MATURING FLUORESCENT PROTEINS AND METHODS FOR USING THE SAME 审中-公开
公开(公告)号：WO2003054158A3
公开(公告)日：2003-07-03
申请号：PCT/US2002/040539
申请日：2002-12-18
申请人： THE UNIVERSITY OF CHICAGO , BEVIS, Brooke , GLICK, Benjamin
发明人： BEVIS, Brooke , GLICK, Benjamin
IPC分类号： C07H21/04
摘要： Nucleic acid compositions encoding rapidly maturing fluorescent proteins, as well as non-aggregating versions thereof (and mutants thereof) as well as the proteins encoding the same, are provided. The proteins of interest are proteins that are fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that, in certain embodiments, they are mutants of wild type proteins that are obtained either from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from Anthozoan non-Pennatulacean (sea pen) species. In certain embodiments, the subject proteins are mutants of wild type Discosoma sp. "red" fluorescent protein. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

3. 发明申请

WO2003054158A2 RAPIDLY MATURING FLUORESCENT PROTEINS AND METHODS FOR USING THE SAME 审中-公开
标题翻译：快速成熟的荧光蛋白及其使用方法
公开(公告)号：WO2003054158A2
公开(公告)日：2003-07-03
申请号：PCT/US2002/040539
申请日：2002-12-18
申请人： THE UNIVERSITY OF CHICAGO , BEVIS, Brooke , GLICK, Benjamin
发明人： BEVIS, Brooke , GLICK, Benjamin
IPC分类号： C12N
CPC分类号： C07K14/43595 , A01K67/0275 , A61K38/00 , C12N15/8257 , G01N33/582
摘要： Nucleic acid compositions encoding rapidly maturing fluorescent proteins, as well as non-aggregating versions thereof (and mutants thereof) as well as the proteins encoding the same, are provided. The proteins of interest are proteins that are fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that, in certain embodiments, they are mutants of wild type proteins that are obtained either from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from Anthozoan non-Pennatulacean (sea pen) species. In certain embodiments, the subject proteins are mutants of wild type Discosoma sp. "red" fluorescent protein. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
摘要翻译：提供了编码快速成熟的荧光蛋白的核酸组合物，以及其非聚集形式（及其突变体）以及编码它们的蛋白质。感兴趣的蛋白质是荧光的蛋白质，其中该特征来源于蛋白质的两个或更多个残基的相互作用。主题蛋白质的进一步特征在于，在某些实施方案中，它们是野生型蛋白质的突变体，其可以从非生物发光虫螨（例如，珊瑚虫）物种获得，或者从非生物珊瑚虫（Pennatulacean）物种获得。在某些实施方案中，主题蛋白质是野生型Discosoma sp。的突变体。＆QUOT;红色＆QUOT; 荧光蛋白。另外感兴趣的是与上述特定蛋白质基本相似或突变体的蛋白质。还提供了核酸的片段和由其编码的肽，以及针对主题蛋白质和转基因细胞和生物体的抗体。目标蛋白质和核酸组合物可用于各种不同的应用。最后，提供用于这种应用的试剂盒，例如包含目标核酸组合物的试剂盒。

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