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1. 发明申请

WO2016144973A1 PORE-FORMING PROTEIN CONJUGATE COMPOSITIONS AND METHODS 审中-公开
标题翻译：形成蛋白质的蛋白质组合物和方法
公开(公告)号：WO2016144973A1
公开(公告)日：2016-09-15
申请号：PCT/US2016/021396
申请日：2016-03-08
申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK , LI, Zengmin , KALACHIKOV, Sergey , JU, Jingyue , FULLER, Carl, W.
发明人： LI, Zengmin , KALACHIKOV, Sergey , JU, Jingyue , FULLER, Carl, W.
IPC分类号： A61K47/48 , C07D237/26 , C07D403/04
CPC分类号： C12Q1/6874 , A61K47/545 , A61K47/62 , A61K47/6889 , A61K47/69 , C07K14/31 , C12N9/1252 , C12N9/96 , C12Y207/07007
摘要： The present disclosure relates to compositions and methods based on a fast, efficient chemical reaction for conjugating a pore- forming protein, such as α-hemolysin, to a biomolecule, such as antibodies, receptors, and enzymes, such as DNA polymerase, and the use of such pore-forming protein conjugates in nanopore devices and methods.
摘要翻译：本公开涉及基于用于将成孔蛋白（例如α-溶血素）与生物分子（例如抗体，受体和酶如DNA聚合酶）共轭的快速有效的化学反应的组合物和方法，以及在纳米孔装置和方法中使用这种造孔蛋白缀合物。

2. 发明申请

WO2020181129A1 HIGH ACCURACY NANOPORE-BASED SINGLE MOLECULE SEQUENCING BY SYNTHESIS WITH TAGGED NUCLEOTIDES 审中-公开
公开(公告)号：WO2020181129A1
公开(公告)日：2020-09-10
申请号：PCT/US2020/021253
申请日：2020-03-05
申请人： THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK , JU, Jingyue , FULLER, Carl, W. , KUMAR, Shiv , KALACHIKOV, Sergey , LI, Xiaoxu , MOROZOVA, Irina , RUSSO, James , JOCKUSCH, Steffen
发明人： JU, Jingyue , FULLER, Carl, W. , KUMAR, Shiv , KALACHIKOV, Sergey , LI, Xiaoxu , MOROZOVA, Irina , RUSSO, James , JOCKUSCH, Steffen
IPC分类号： G01N33/487 , C12Q1/6869 , C12Q1/6874 , C12N9/12 , C12M1/34 , C12P19/34 , C07H21/04
摘要： Disclosed herein are highly accurate approaches for single molecule electronic nanopore-based SBS.

3. 发明申请

WO2015148402A1 CHEMICAL METHODS FOR PRODUCING TAGGED NUCLEOTIDES 审中-公开
标题翻译：用于生产标记核酸的化学方法
公开(公告)号：WO2015148402A1
公开(公告)日：2015-10-01
申请号：PCT/US2015/022063
申请日：2015-03-23
申请人： THE TRUSTEES OF COLUMBIA UNIVERISTY IN THE CITY OF NEW YORK , GENIA TECHNOLOGIES, INC. , FULLER, Carl, W. , KUMAR, Shiv , JU, Jingyue , DAVIS, Randall , CHEN, Roger
发明人： FULLER, Carl, W. , KUMAR, Shiv , JU, Jingyue , DAVIS, Randall , CHEN, Roger
IPC分类号： C07H19/04
CPC分类号： C12Q1/6874 , C07H17/02 , C07H19/10 , C07H19/20 , C12Q1/6806
摘要： This disclosure provides systems and methods for attaching nano pore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disdosed tagged nudeotides. Provided herein are nudeotides with attached tags and methods for attaching tags to nudeotides. The tags can be attached by chemical reactions, such as "dick chemistry". In an aspect, the present disdosure provides a tagged nudeotide, comprising: (a) a poly-phosphate moiety having a terminal phosphate; and (b) a tag covalently coupled to the terminal phosphate of the nudeotide by a triazole, a 1,2-diazine, a disulfide, a secondary amine, a hydrazone, a thio-acetamide, or a maleimide-thioadduct.
摘要翻译：本公开提供了将纳米孔可检测标签附着于核苷酸的系统和方法。本公开还提供了使用剔除的标记的核苷酸对核酸进行测序的方法。本文提供了具有附加标签的核苷酸和将标签附着到裸核苷酸的方法。标签可以通过化学反应来附着，例如“dick chemistry”。在一方面，本发明提供了一种标记的核苷酸，其包含：（a）具有末端磷酸酯的聚磷酸酯部分; 和（b）通过三唑，1,2-二嗪，二硫化物，仲胺，腙，硫代乙酰胺或马来酰亚胺 - 硫代加成酸共价偶联到核苷酸的末端磷酸酯上的标签。

4. 发明申请

WO2006073892A3 PHI 15 DNA POLYMERASE 审中-公开
公开(公告)号：WO2006073892A3
公开(公告)日：2006-07-13
申请号：PCT/US2005/046799
申请日：2005-12-22
申请人： GE HEALTHCARE BIO-SCIENCES CORP. , FULLER, Carl, W. , LAM, Cuong
发明人： FULLER, Carl, W. , LAM, Cuong
IPC分类号： C12N9/12 , C12N15/54 , C12P21/02 , C12Q1/68
摘要： A purified recombinant DNA polymerase having high processivity and strand-displacement activity. An isolated nucleic acid that encodes the bacteriophage DNA polymerase. A kit and method for amplifying DNA is also disclosed.

5. 发明申请

WO2007076461A1 THERMOSTABLE DNA POLYMERASE FROM THERMUS SCOTODUCTUS 审中-公开
标题翻译：热稳定DNA聚合酶
公开(公告)号：WO2007076461A1
公开(公告)日：2007-07-05
申请号：PCT/US2006/062528
申请日：2006-12-22
申请人： GE HEALTHCARE BIO-SCIENCES CORP. , RECH, Renee , HAMILTON, Scott , FULLER, Carl, W.
发明人： RECH, Renee , HAMILTON, Scott , FULLER, Carl, W.
IPC分类号： C12N15/54 , C12N15/63 , C12N9/12 , C12N1/21 , C12Q1/68
CPC分类号： C12N9/1252
摘要： Disclosed is a thermostable DNA polymerase from Thermus scotoductus . This enzyme is useful for procedures requiring strand-displacing DNA synthesis such as SDA, for DNA sequencing, reverse transcription and polymerase chain reaction. Included within the scope of the present invention are various mutants (deletion and substitution) that retain thermostability and the ability to replicate DNA with substantially the same efficiency as the native Thermus scotoductus polymerase.
摘要翻译：公开了来自Thermus scotoductus的热稳定DNA聚合酶。该酶可用于需要链置换DNA合成的方法，如SDA，用于DNA测序，逆转录和聚合酶链反应。在本发明的范围内包括保留热稳定性的各种突变体（缺失和取代）以及以与天然的Thermus scotoductus聚合酶基本相同的效率复制DNA的能力。

6. 发明申请

WO2005123957A2 METHOD FOR NUCLEIC ACID ANALYSIS 审中-公开
标题翻译：核酸分析方法
公开(公告)号：WO2005123957A2
公开(公告)日：2005-12-29
申请号：PCT/US2005/020378
申请日：2005-06-09
申请人： AMERSHAM BIOSCIENCES CORP , FULLER, Carl, W. , NELSON, John, R.
发明人： FULLER, Carl, W. , NELSON, John, R.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6869 , C12Q2565/519 , C12Q2565/301 , C12Q2563/137 , C12Q2527/127 , C12Q2523/113
摘要： This invention provides methods for nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the labeled nucleotide complementary to the next template nucleotide in the closed complex. Detection of the label allows determination of the identity of this next correct nucleotide. Identification can be either in place, as part of the complex, or as the dye is eluted from the complex when the reaction cycle is completed by the addition of divalent metal ion. In this way, sequential nucleotides of a DNA can be identified, effectively determining the DNA sequence. This method can be applied to nucleic acid single molecules or to collections of identical or nearly identical sequence such as PCR products or clones. Multiple templates can be sequenced in parallel, particularly if they are immobilized on a solid support.
摘要翻译：本发明提供核酸分析方法。可以在聚合酶反应期间形成核酸模板，核苷酸和聚合酶的闭合复合物，不存在二价金属离子。这用于捕获与封闭复合物中下一个模板核苷酸互补的标记核苷酸。标签的检测允许确定下一个正确的核苷酸的身份。识别可以作为复合物的一部分就位，或当通过添加二价金属离子完成反应循环时染料从络合物中洗脱出来。以这种方式，可以鉴定DNA的连续核苷酸，有效地确定DNA序列。该方法可以应用于核酸单分子或相同或几乎相同序列的集合，例如PCR产物或克隆。多个模板可以并行测序，特别是如果它们固定在固体支持物上。

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