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1. 发明申请

WO0248776A2 OPTICAL SCANNING SYSTEM APPARATUS 审中-公开
标题翻译：光学扫描系统设备
公开(公告)号：WO0248776A2
公开(公告)日：2002-06-20
申请号：PCT/GB0105565
申请日：2001-12-14
申请人： THE TECHNOLOGY PARTNERSHIP PLC , WITHERS MICHAEL , BILLINGTON DAVID , CASSELLS JOHN
发明人： WITHERS MICHAEL , BILLINGTON DAVID , CASSELLS JOHN
IPC分类号： G02B21/26 , B01L3/00 , G02B21/00 , G02B21/34
CPC分类号： B01L3/5085 , G02B21/34
摘要： An apparatus for use with an optical scanning or observation system. The apparatus comprises a drawer for receiving, in use, a sample carrier to be scanned or observed. A first clamp member, engages, in use, with one surface of a sample carrier in the drawer. A second clamp member is arranged to engage, in use, with the side of the sample carrier opposite to that of the first clamp member, the second clamp member having an optical window formed therein to enable viewing, in use, of a sample carrier. The first and second clamping members are arranged in use to move the sample carrier out of engagement with the drawer so that the sample carrier is supported solely on the clamping members.
摘要翻译：一种用于光学扫描或观察系统的设备。该设备包括用于在使用中接收待扫描或观察的样本载体的抽屉。第一夹紧构件在使用中与抽屉中的样品载体的一个表面接合。第二夹紧构件布置成在使用中与样品载体的与第一夹紧构件的侧面相反的侧面接合，第二夹紧构件具有形成在其中的光学窗口，以能够在使用中观察样品载体。第一和第二夹紧构件被设置成在使用中移动样品载体使其脱离与抽屉的接合，使得样品载体仅被支撑在夹紧构件上。

2. 发明申请

WO02073198A3 NON-SEPARATION ASSAY AND SYSTEM USING OPAQUE PARTICLES 审中-公开
标题翻译：非分离测试和使用OPAQUE颗粒的系统
公开(公告)号：WO02073198A3
公开(公告)日：2003-05-30
申请号：PCT/GB0200984
申请日：2002-03-08
申请人： THE TECHNOLOGY PARTNERSHIP PLC , COPE TRISTAN JOHN , CASSELLS JOHN
发明人： COPE TRISTAN JOHN , CASSELLS JOHN
IPC分类号： G01N33/53 , G01N33/542 , G01N33/543 , G01N33/58
CPC分类号： G01N33/582 , G01N33/5302 , G01N33/542 , G01N33/54326 , G01N33/585
摘要： A method for performing a non-separation assay for determining the level of binding of one component to another. A first component is provided incorporating a fluorescent probe dissolved or suspended in solution. A substantially opaque particle is provided onto or into which is incorporated binding sites for the first component and optionally incorporating a dye or fluorophore of different emission spectrum to the first component. The opaque particle is immersed in a solution or suspension of the first component, and the opaque particle is allowed to settle out of the solution, or be transported to a fixed position by an applied force. The solution and opaque particle are illustrated with a beam of light such that the opaque particle is in the foreground and attenuates and illuminating beam before it passes into the solution beyond. The intensity of received light (fluorescence) from the first component over an area of the sample with an imaging or scanning detector from the same side of the sample as the illuminating light is determined, and the position of the second component in the sample is determined by detecting attenuation of the received light from the sample and/or by detecting the presence of received light from a dye incorporated in the second component. An apparatus, as well as opaque particles for performing the method are also provided.
摘要翻译：一种用于进行非分离测定以确定一种组分与另一种组分的结合水平的方法。提供了溶解或悬浮在溶液中的荧光探针的第一组分。提供基本上不透明的颗粒，其中引入第一组分的结合位点，并且任选地将不同发射光谱的染料或荧光团掺入第一组分。将不透明颗粒浸入第一组分的溶液或悬浮液中，并使不透明颗粒从溶液中沉淀出来，或通过施加的力将其输送到固定位置。溶液和不透明颗粒用光束示出，使得不透明颗粒处于前景，并在其进入溶液之前衰减和照射光束。确定来自与照明光的样品相同侧的成像或扫描检测器的来自样品的区域的第一成分的接收光（荧光）的强度，并且确定样品中第二成分的位置通过检测来自样品的接收光的衰减和/或通过检测来自掺入第二组分的染料的接收光的存在。还提供了一种装置，以及用于执行该方法的不透明颗粒。

3. 发明申请

WO0163245A3 METHOD AND APPARATUS FOR HIGH THROUGHPUT CELL - BASED ASSAYS FOR SCREENING AND DIAGNOSTICS 审中-公开
标题翻译：用于筛选和诊断的基于高通量细胞的测定方法和装置
公开(公告)号：WO0163245A3
公开(公告)日：2002-03-14
申请号：PCT/GB0100804
申请日：2001-02-23
申请人： THE TECHNOLOGY PARTNERSHIP PLC , CASSELLS JOHN , MARTIN PAUL , DISLEY DARRIN
发明人： CASSELLS JOHN , MARTIN PAUL , DISLEY DARRIN
IPC分类号： G01N21/64 , C12M1/00 , C12Q1/00 , G01N15/10 , G01N15/14 , G01N33/48 , G01N33/483 , G06K9/00
CPC分类号： G01N15/1475 , G01N2015/1006 , G06K9/00127
摘要： A method of performing an assay to detect and quantify changes in morphology or intracellular events in living or dead cells. The method comprises the step of presenting the cells on a surface for analysis in a single sample or array of samples and providing a means of fluorescently labelling cell structures, engulfed or associated particles or molecules contained on or within the cells. The cells are scanned with a detection system to obtain a linearseries of intensity values at intervals of 10 microns or less across each cell to produce line amplitude data in at least one emission band. A threshold algorithm is applied to determine the beginning and end of each feature on the line amplitude data, whereina feature is any perturbation from a determined background signal. The determined line amplitude data is processed for each feature for one or more emission bands to generate a value for at leastone of area specific intensity, peak intensity, half-width, half-width specific intensity, total intensity, peak intensity, peak ratio, inflection ratio, ID gaussian fit, 2D gaussian fit or a mathematical combination of these values.
摘要翻译：进行测定以检测和量化活细胞或死细胞中形态或细胞内事件变化的方法。该方法包括在单个样品或样品阵列中的表面上呈现细胞以进行分析的步骤，并提供荧光标记细胞上包含的细胞结构，吞噬的或相关的颗粒或分子的方法。用检测系统扫描细胞以在每个细胞上获得10微米或更小的间隔的强度值的线性序列，以在至少一个发射带中产生线幅度数据。应用阈值算法来确定线幅度数据上每个特征的开始和结束，其中特征是来自确定的背景信号的任何扰动。对于一个或多个发射带的每个特征处理所确定的线幅数据，以产生区域特定强度，峰强度，半宽度，半宽比特征强度，总强度，峰强度，峰值比，弯曲比，ID高斯拟合，2D高斯拟合或这些值的数学组合。

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