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1. 发明申请

WO2007120183A2 A MICRO-BUBBLE PLATE FOR PATTERNING BIOLOGICAL AND NON-BIOLOGICAL MATERIALS 审中-公开
标题翻译：用于绘制生物和非生物材料的微孔板
公开(公告)号：WO2007120183A2
公开(公告)日：2007-10-25
申请号：PCT/US2006/039929
申请日：2006-10-10
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , WANG, Yuli , BACHMAN, Mark , SIMS, Christopher , LI, Guann-pyng , ALLBRITTON, Nancy
发明人： WANG, Yuli , BACHMAN, Mark , SIMS, Christopher , LI, Guann-pyng , ALLBRITTON, Nancy
IPC分类号： C12M1/22
CPC分类号： B01L3/5085 , B01L3/5088 , B01L2200/0642 , B01L2200/12 , B01L2300/0819 , C12M25/06 , C12M29/20
摘要： Systems and methods for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiments of the present invention include a method for placing materials on the plate at predetermined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micromechanical structures where the structures may be formed from living or nonliving material, organic or inorganic, and the like.
摘要翻译：用于在板上的特定位置图案化生物和非生物材料的系统和方法，以及生长三维结构。优选实施例包括具有区域的板，当板被浸没在液体中时，其具有通常以气泡形式捕获气体的区域。本发明的其它实施例包括通过使用截留气体将材料放置在预定位置处的材料的方法，以防止材料在不需要的区域收集。该板对于在特定位置（例如阵列）上的电镀细胞和组织具有很大的用途。所公开的方法还可以用于在特定位置涂覆板的表面用于图案化涂布应用，并且建立材料以产生三维结构，包括微机械结构，其中结构可以由活的或非活性的材料形成，有机或无机，等等。

2. 发明申请

WO2006041938A2 MICROPATTERNED PLATE WITH MICRO-PALLETS FOR ADDRESSABLE BIOCHEMICAL ANALYSIS 审中-公开
标题翻译：用于可寻址生物化学分析的微型玻璃微波炉
公开(公告)号：WO2006041938A2
公开(公告)日：2006-04-20
申请号：PCT/US2005/035860
申请日：2005-10-04
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , BACHMAN, Mark , LI, Guann-Pyng , ALLBRITTON, Nancy , SIMS, Christopher
发明人： BACHMAN, Mark , LI, Guann-Pyng , ALLBRITTON, Nancy , SIMS, Christopher
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L3/5085 , B01J19/0046 , B01J2219/00315 , B01J2219/00441 , B01J2219/00527 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00619 , B01J2219/00621 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00689 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B01L3/508 , B01L2300/0636 , B01L2300/0819 , B01L2300/0829
摘要： A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses.
摘要翻译：制造的板可使细胞，微生物，蛋白质，DNA，生物分子和其他生物培养基的样品定位在板上的特定位置或位点，以便对样品进行可寻址分析。优选地，一些或所有的部位由可移除的材料或托盘构建，使得感兴趣的样品的子集可以容易地与板分离以用于进一步的处理或分析。该板可以包含增强或促进样品的附着和/或功能的结构或化学处理，并且促进或辅助其分析。

3. 发明申请

WO2011103143A1 ARRAY OF MICROMOLDED STRUCTURES FOR SORTING ADHERENT CELLS 审中-公开
标题翻译：用于分离附着细胞的微结构阵列
公开(公告)号：WO2011103143A1
公开(公告)日：2011-08-25
申请号：PCT/US2011/025018
申请日：2011-02-16
申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , ALLBRITTON, Nancy , SIMS, Christopher , WANG, Yuli
发明人： ALLBRITTON, Nancy , SIMS, Christopher , WANG, Yuli
IPC分类号： C12M3/00
CPC分类号： C12M23/12 , B01J19/0046 , B01J2219/00452 , B01J2219/00743 , C12M23/20 , C12M23/22 , C12M25/04 , C12M25/16 , C12M47/02 , C12M47/04
摘要： An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).
摘要翻译：用于收集或培养细胞或细胞集落的装置包括：由柔性弹性聚合物材料形成且在其中形成有多个孔的共同基底; 以及可释放地连接到所述公共基板的多个刚性电池载体，所述载体以阵列的形式布置，并且每个载体弹性地容纳在其中一个孔中。使用这种装置收集或培养细胞或细胞集落的方法是通过在装置上沉积携带细胞的液体培养基进行的，以使所述细胞沉降或粘附到所述载体上; 然后（c）通过从其被接收的空腔（例如通过用探针推动）逐渐地将释放能量释放到每个载体上来释放至少一个具有所述电池的所选载流子。

4. 发明申请

WO2010068743A1 SYSTEMS AND METHODS FOR ISOLATING CELLS IN CELL COLONIES IN CULTURE 审中-公开
标题翻译：用于在细胞培养中分离细胞的系统和方法
公开(公告)号：WO2010068743A1
公开(公告)日：2010-06-17
申请号：PCT/US2009/067482
申请日：2009-12-10
申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , ALLBRITTON, Nancy , SIMS, Christopher , XU, Wei , SHADPOUR, Hamed
发明人： ALLBRITTON, Nancy , SIMS, Christopher , XU, Wei , SHADPOUR, Hamed
IPC分类号： C12Q1/24
CPC分类号： C12Q1/24 , B01L3/5025 , B01L3/5085 , B01L2200/0668 , B01L2300/0609 , B01L2300/0819
摘要： Selecting and propagating a cell colony of interest from among a plurality of cell colonies carried on a common substrate in culture is carried out by: (a) selecting a cell colony of interest from among the plurality of cell colonies; (b) isolating a cell subset from the cell colony of interest; (c) analyzing (for example, by a destructive analysis) the cell subset isolated from the cell colony of interest to confirm the presence or absence of a desired feature therein; and then (d) propagating the cell colony of interest when the desired feature is present in the cell subset. A micropallet apparatus may include: (a) a substrate; (b) a plurality of discrete arrays formed on the substrate, each of the arrays comprising a plurality of releasable pallets, and (c) a plurality of gap forming regions, wherein the gap forming regions surround the pallets and separate the pallets from one another.
摘要翻译：通过以下步骤进行选择和繁殖培养中携带在共同底物上的多个细胞集落中的感兴趣的细胞集落：（a）从多个细胞集落中选择感兴趣的细胞集落; （b）从感兴趣的细胞集落分离细胞亚群; （c）分析（例如，通过破坏性分析）从感兴趣的细胞集落分离的细胞亚群以确认其中存在或不存在所需特征; 然后（d）当所需特征存在于细胞子集中时，传播感兴趣的细胞集落。微孔装置可以包括：（a）基底; （b）形成在所述基板上的多个分立阵列，每个所述阵列包括多个可释放的托盘，以及（c）多个间隙形成区域，其中所述间隙形成区域围绕所述托盘并将所述托盘彼此分离。

5. 发明申请

WO2010016916A3 METHOD OF CREATING REMOVABLE BARRIERS IN MICROFABRICATED FLUIDIC DEVICES 审中-公开
公开(公告)号：WO2010016916A3
公开(公告)日：2010-02-11
申请号：PCT/US2009/004510
申请日：2009-08-06
申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , ALLBRITTON, Nancy , SIMS, Christopher , XU, Wei , BEAR, James , UETRECHT, Andrea
发明人： ALLBRITTON, Nancy , SIMS, Christopher , XU, Wei , BEAR, James , UETRECHT, Andrea
IPC分类号： G01N35/00 , G01N37/00 , C12Q1/02
摘要： A method for creating a removable barrier in a microfluidic apparatus is carried out by: (a) providing a microfluidic apparatus having at least a first chamber and a second chamber, each including at least an inlet, wherein the first and second chamber are separated from one another by a plurality of barrier elements, the plurality of barrier elements defining a barrier zone, (b) introducing a liquid sample into the apparatus through the inlet of the first or second chamber to create a retained continuous liquid phase in one chamber, a continuous gas phase in the other chamber, and a gas/liquid interface between the first and second chamber at the barrier zone, (c) releasing the interface from the barrier elements, and (d) advancing the liquid phase past the barrier zone into the gas chamber displacing the gas phase. In some embodiments the barrier elements are hydrophobic (for example, the barrier elements comprise a hydrophobic polymer. Devices such as chemotaxis devices incorporating such methods are also described.

6. 发明申请

WO2010093766A1 METHOD AND DEVICE FOR CELL SELECTION AND COLLECTION IN AN ISOLATED CULTURING ENVIRONMENT 审中-公开
标题翻译：用于分离培养环境中细胞选择和收集的方法和装置
公开(公告)号：WO2010093766A1
公开(公告)日：2010-08-19
申请号：PCT/US2010/023854
申请日：2010-02-11
申请人： THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL , ALLBRITTON, Nancy , SIMS, Christopher , XU, Wei
发明人： ALLBRITTON, Nancy , SIMS, Christopher , XU, Wei
IPC分类号： C12M1/22
CPC分类号： C12M25/04 , C12M23/16
摘要： An apparatus for collecting or culturing cells or cell colonies has a common substrate and a plurality of cell carriers releasably connected to the common substrate. The carriers are arranged in the form of an array. The invention employs microcups as the cell carriers. The substrate is preferably free of barriers between the microcups, and in some embodiments the microcups have porous walls. Methods of using the apparatus are also described.
摘要翻译：用于收集或培养细胞或细胞集落的装置具有公共基底和可释放地连接到共同基底的多个细胞载体。载体以阵列的形式排列。本发明采用微型杯作为细胞载体。基底优选在微杯之间不存在阻挡层，并且在一些实施例中，微型杯具有多孔壁。还描述了使用该装置的方法。

7. 发明申请

WO2003093791A3 FAST ELECTRICAL LYSIS OF CELLS AND RAPID COLLECTION OF THE CONTENTS THEREOF USING CAPILLARY ELECTROPHORESIS 审中-公开
公开(公告)号：WO2003093791A3
公开(公告)日：2003-11-13
申请号：PCT/US2003/013599
申请日：2003-05-02
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： ALBRITTON, Nancy , LI, Guann-Pyng , BACHMAN, Mark , SIMS, Christopher , HAN, Futian
IPC分类号： B01L3/00
摘要： The invention provides apparatus and methods for subsecond lysis of selected cells (58) in a cell chamber (10) using a voltage pulse of 10 ms to 10 µs in duration followed by nearly simultaneous collection of the lysed cellular contents (59) into a capillary electrophoresis tube (42) or other suitable micro-collection device (15). Cell chambers (10) and capillary electrophoresis (42) tubes configured with electrodes (18, 19) for performing the electrical lysis are described. The influence of variables that govern the rate of cell lysis, such as the inter-electrode distance, pulse duration, and pulse strength are also described. The methods are illustrated using fluorophores that are loaded into a cell (10) and then collected following electrical lysis, separated by electrophoresis, and then detected by laser-induced fluorescence detection in a capillary electrophoresis system.

8. 发明申请

WO2002092199A1 METHOD AND APPARATUS FOR DETECTING CANCEROUS CELLS USING MOLECULES THAT CHANGE ELECTROPHORETIC MOBILITY 审中-公开
标题翻译：使用电视机改变电泳移动的检测粪便细胞的方法和装置
公开(公告)号：WO2002092199A1
公开(公告)日：2002-11-21
申请号：PCT/US2002/014755
申请日：2002-05-09
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： ALLBRITTON, Nancy , SIMS, Christopher
IPC分类号： B01D57/02
CPC分类号： C12Q1/00 , B01D53/62 , C12Q1/485 , G01N27/44721 , G01N27/44743 , G01N33/50 , G01N33/5017
摘要： The activity of encogenic intercellular chemical reactions of molecules is measured by the use of fluorescently labeled substrate molecules that underdo a change in electrophoretic mobility upon a chemical reaction such as that catalyzed by an enzyme or kinase. Specificity is achieved by using labeled substrate molecules that can be acted upon only by specific oncogenic enzymes can be determined. Measurements are made with the intercellular presence of such substrate molecules, at some time of interest. To ensure accuracy, measurements must be made in a timely manner so as to minimize chemical reactions occuring subsequent to the time of interest. Fast controllable laser lysis is used to obtain the contents of said cell or cells into which reporter substrate molecules have been introduced. The cell contents are then subjected to capillary electrophoresis and oncogenic enzymatic activity is determined by comparing amounts of unaltered substrate molecules to the amounts of altered substrate molecules which are separated by the electrophoresis and identified by the presence of a fluorescent label.
摘要翻译：通过使用荧光标记的底物分子来测量分子的细胞间化学反应的活性，所述底物分子在诸如由酶或激酶催化的化学反应引起的电泳迁移率发生变化。通过使用可以仅通过特异性致癌酶作用的标记的底物分子来实现特异性。在感兴趣的某个时间，用这种底物分子的细胞间存在进行测量。为了确保准确性，必须及时进行测量，以尽可能减少感兴趣的时间后发生的化学反应。使用快速可控的激光裂解来获得引入了报告基质分子的所述细胞或细胞的内容物。然后对细胞内容物进行毛细管电泳，并且通过将未改变的底物分子的量与通过电泳分离并通过荧光标记的存在鉴定的改变的底物分子的量进行比较来确定致癌酶活性。

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