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1. 发明申请

WO2002093131A3 METHODS FOR ISOLATING AND LABELING SAMPLE MOLECULES 审中-公开
公开(公告)号：WO2002093131A3
公开(公告)日：2002-11-21
申请号：PCT/US2002/015500
申请日：2002-05-14
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , UNIVERSITY OF WASHINGTON , APPLERA CORPORATION , AEBERSOLD, Rudolf, H. , ZHOU, Huilin , RIST, Beate , VELLA, George, J. , PURKAYASTHA, Subhasish , PILLAI, Sasi
发明人： AEBERSOLD, Rudolf, H. , ZHOU, Huilin , RIST, Beate , VELLA, George, J. , PURKAYASTHA, Subhasish , PILLAI, Sasi
IPC分类号： G01N24/00
摘要： The invention provides methods for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups, and a reactive group for the sample molecule, under conditions allowing the sample molecule to covalently bind to the reactive group; and cleaving the cleavable functional group, thereby releasing the sample molecule comprising the one or more functional groups, which can be a tag. The invention also provides a solid support covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are positioned relative to each other to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group.

2. 发明申请

WO2002093131A2 METHODS FOR ISOLATING AND LABELING SAMPLE MOLECULES 审中-公开
标题翻译：分离和标记样品分子的方法
公开(公告)号：WO2002093131A2
公开(公告)日：2002-11-21
申请号：PCT/US2002/015500
申请日：2002-05-14
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , AEBERSOLD, Rudolf, H. , ZHOU, Huilin
发明人： AEBERSOLD, Rudolf, H. , ZHOU, Huilin
IPC分类号： G01N
CPC分类号： G01N33/533 , G01N33/532 , G01N33/58 , G01N33/582 , G01N2458/15 , Y10T436/24
摘要： The invention provides methods for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups, and a reactive group for the sample molecule, under conditions allowing the sample molecule to covalently bind to the reactive group; and cleaving the cleavable functional group, thereby releasing the sample molecule comprising the one or more functional groups, which can be a tag. The invention also provides a solid support covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are positioned relative to each other to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group.
摘要翻译：本发明提供了通过使样品分子与固体支持物接触的方法，所述固体支持物与样品分子在允许样品分子形成的条件下连接至包含可切割官能团，一个或多个官能团和反应性基团的化学基团共价结合反应基团; 并切断可切割的官能团，从而释放包含一个或多个可以是标签的官能团的样品分子。本发明还提供了共价偶联到化学基团上的固体支持物，该化学基团包含可切割官能团，质谱标签和用于共价连接样品分子的反应性基团，其中可切割官能团，标签和反应性基团相对于彼此允许在裂解可裂解官能团时将标签转移到样品分子。

3. 发明申请

WO2004019000A2 CHEMICAL REAGENTS AND METHODS FOR DETECTION AND QUANTIFICATION OF PROTEINS IN COMPLEX MIXTURES 审中-公开
标题翻译：用于检测和定量复杂混合物中蛋白质的化学试剂和方法
公开(公告)号：WO2004019000A2
公开(公告)日：2004-03-04
申请号：PCT/US2003/026266
申请日：2003-08-20
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , UNIVERSITY OF WASHINGTON , AEBERSOLD, Rudolf, H. , BOTTARI, Patricia, Q. , GELB, Michael, H. , TURECEK, Frantisek
发明人： AEBERSOLD, Rudolf, H. , BOTTARI, Patricia, Q. , GELB, Michael, H. , TURECEK, Frantisek
IPC分类号： G01N
CPC分类号： G01N33/585 , G01N33/6803 , G01N33/6809
摘要： The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.
摘要翻译：本发明提供了包含亲和标签，可检测部分，接头，同位素标签和反应性基团的试剂。本发明还提供了使用本发明试剂的方法。该方法可用于在允许反应基团结合样品中的一种或多种多肽的条件下通过使样品与本发明的试剂接触来标记样品中的多肽。本发明另外提供了分离，鉴定和定量样品中多肽的方法。本发明还提供了使用本发明的试剂诊断疾病的方法。

4. 发明申请

WO2003102018A2 METHODS FOR QUANTITATIVE PROTEOME ANALYSIS OF GLYCOPROTEINS 审中-公开
标题翻译：糖蛋白定量分析方法
公开(公告)号：WO2003102018A2
公开(公告)日：2003-12-11
申请号：PCT/US2003/017635
申请日：2003-06-03
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , AEBERSOLD, Rudolf, H. , ZHANG, Hui
发明人： AEBERSOLD, Rudolf, H. , ZHANG, Hui
IPC分类号： C07K
CPC分类号： G01N33/6848 , C12Q1/34 , C12Q1/37 , G01N33/68 , G01N33/6803 , G01N33/6842 , G01N33/6851 , G01N2400/00 , G01N2458/15 , Y10T436/24 , Y10T436/25125
摘要： The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method can include the steps of immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method can further include the step of identifying one or more glycopeptides, for example, using mass spectrometry.
摘要翻译：本发明提供了一种用于鉴定和定量样品中的聚糖肽的方法。该方法可以包括将糖多肽固定在固体支持物上的步骤; 切割固定化的糖多肽，从而释放非糖基化肽并保留固定的糖肽; 从固体支持物释放糖肽; 并分析释放的糖肽。该方法还可以包括鉴定一种或多种糖肽的步骤，例如使用质谱法。

5. 发明申请

WO2005007677A3 AFFINITY CAPTURE OF PEPTIDES BY MICROARRAY AND RELATED METHODS 审中-公开
公开(公告)号：WO2005007677A3
公开(公告)日：2005-01-27
申请号：PCT/US2004/022135
申请日：2004-07-09
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , AEBERSOLD, Rudolf, H. , ZHANG, Hui
发明人： AEBERSOLD, Rudolf, H. , ZHANG, Hui
IPC分类号： G01N33/53
摘要： The invention provides methods of detecting polypeptides in a sample. The method can include the steps of cleaving polypeptides in a test sample to generate peptides; adding a predetermined amount of isotopically labeled peptide standards to the cleaved test sample, wherein the peptide standards correspond to peptides cleaved with the same reagent used to cleave the test sample; contacting the cleaved test sample containing peptide standards with an array of immobilized binding agents specific for the peptide standards; washing the array to remove unbound peptides, thereby retaining affinity captured sample peptides and standard peptides; analyzing the affinity captured peptides using mass spectrometry; and determining the presence of bound test peptides and standard peptides. The method can further include the step of quantifying the amount of the test peptides by comparing the ratio of test peptide to corresponding standard peptide.

6. 发明申请

WO2004058051A2 ANDROGEN-REGULATED GENES AND USES FOR DIAGNOSIS, PROGNOSIS AND TREATMENT OF PROSTATE NEOPLASTIC CONDITIONS 审中-公开
标题翻译：用于诊断，预防和治疗前列腺增生症状的原发性基因和用途
公开(公告)号：WO2004058051A2
公开(公告)日：2004-07-15
申请号：PCT/US2003/040741
申请日：2003-12-19
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , AEBERSOLD, Rudolf, H. , WRIGHT, Michael, E.
发明人： AEBERSOLD, Rudolf, H. , WRIGHT, Michael, E.
IPC分类号： A61B
CPC分类号： G01N33/57434 , G01N2333/726 , G01N2500/00
摘要： The invention provides a method for diagnosing or predicting susceptibility to a prostate neoplastic condition in an individual. The method involves (a) determining a level of RDC1 in a sample from the individual, and (b) comparing the level of RDC1 in the sample to a reference level of RDC1, wherein a level of RDCl in the sample 2-fold or more higher than the reference level indicates the presence of,or susceptibility to, a prostate neoplastic condi tion in the individual.
摘要翻译：本发明提供了用于诊断或预测个体对前列腺肿瘤病症的易感性的方法。该方法包括（a）确定来自个体的样品中RDC1的水平，和（b）将样品中RDC1的水平与RDC1的参考水平进行比较，其中样品中的RDCl水平为2倍以上高于参考水平表明个体中前列腺肿瘤病症的存在或易感性。

7. 发明申请

WO2008066629A2 METHODS FOR QUANTITATIVE PROTEOME ANALYSIS OF GLYCOPROTEINS 审中-公开
标题翻译：糖蛋白定量分析方法
公开(公告)号：WO2008066629A2
公开(公告)日：2008-06-05
申请号：PCT/US2007/022624
申请日：2007-10-24
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , AEBERSOLD, Rudolf, H. , ZHANG, Hui , HOOD, Leroy, E. , SUN, Bingyun
发明人： AEBERSOLD, Rudolf, H. , ZHANG, Hui , HOOD, Leroy, E. , SUN, Bingyun
IPC分类号： G01N33/00 , G01N33/53 , G01N37/00
CPC分类号： C12Q1/34 , C12Q1/37 , G01N33/68 , G01N33/6803 , G01N33/6842 , G01N33/6848 , G01N33/6851 , Y10T436/13
摘要： The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method can include the steps of immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method can further include the step of identifying one or more glycopeptides, for example, using mass spectrometry.
摘要翻译：本发明提供了一种用于鉴定和定量样品中的聚糖肽的方法。该方法可以包括将糖多肽固定在固体支持物上的步骤; 切割固定化的糖多肽，从而释放非糖基化肽并保留固定的糖肽; 从固体支持物释放糖肽; 并分析释放的糖肽。该方法还可以包括鉴定一种或多种糖肽的步骤，例如使用质谱法。

8. 发明申请

WO2003102220A3 METHODS FOR HIGH THROUGHPUT AND QUANTITATIVE PROTEOME ANALYSIS 审中-公开
公开(公告)号：WO2003102220A3
公开(公告)日：2003-12-11
申请号：PCT/US2003/017686
申请日：2003-06-04
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , AEBERSOLD, Rudolf, H.
发明人： AEBERSOLD, Rudolf, H.
IPC分类号： C12N13/00
摘要： The invention provides methods for identifying and quantifying polypeptides in a sample. The methods include the steps of labeling peptides in a polypeptide sample with an isotope tag; adding a plurality of peptide standards to the polypeptide sample, wherein the peptide standards are labeled with an isotopically distinct version of the isotope tag; resolving the labeled sample and standard peptides into a plurality of fractions, analyzing the resolved fractions using mass spectrometry, identifying an isotope-tagged sample peptide in an analyzed fraction; and determining the amount of the identified isotope-tagged sample peptide in the analyzed fraction by comparison to the amount of isotope tagged standard peptide in the same fraction.

9. 发明申请

WO2003102220A2 METHODS FOR HIGH THROUGHPUT AND QUANTITATIVE PROTEOME ANALYSIS 审中-公开
标题翻译：高通量和定量保护分析方法
公开(公告)号：WO2003102220A2
公开(公告)日：2003-12-11
申请号：PCT/US2003/017686
申请日：2003-06-04
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , AEBERSOLD, Rudolf, H.
发明人： AEBERSOLD, Rudolf, H.
IPC分类号： C12Q
CPC分类号： G01N33/6848 , G01N33/60 , G01N33/6803 , G01N33/6818 , G01N33/6842
摘要： The invention provides methods for identifying and quantifying polypeptides in a sample. The methods include the steps of labeling peptides in a polypeptide sample with an isotope tag; adding a plurality of peptide standards to the polypeptide sample, wherein the peptide standards are labeled with an isotopically distinct version of the isotope tag; resolving the labeled sample and standard peptides into a plurality of fractions, analyzing the resolved fractions using mass spectrometry, identifying an isotope-tagged sample peptide in an analyzed fraction; and determining the amount of the identified isotope-tagged sample peptide in the analyzed fraction by comparison to the amount of isotope tagged standard peptide in the same fraction.
摘要翻译：本发明提供了用于鉴定和定量样品中多肽的方法。所述方法包括在具有同位素标签的多肽样品中标记肽的步骤; 向所述多肽样品中加入多个肽标准物，其中所述肽标准物用同位素不同型号的同位素标签标记; 将标记的样品和标准肽分解成多个级分，使用质谱分析分辨级分，在分析的级分中鉴定同位素标记的样品肽; 并通过与相同级分中的同位素标记的标准肽的量进行比较来确定所分析的级分中鉴定的同位素标记的样品肽的量。

10. 发明申请

WO2003102018A3 METHODS FOR QUANTITATIVE PROTEOME ANALYSIS OF GLYCOPROTEINS 审中-公开
公开(公告)号：WO2003102018A3
公开(公告)日：2003-12-11
申请号：PCT/US2003/017635
申请日：2003-06-03
申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY , AEBERSOLD, Rudolf, H. , ZHANG, Hui
发明人： AEBERSOLD, Rudolf, H. , ZHANG, Hui
IPC分类号： G01N33/53
摘要： The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method can include the steps of immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method can further include the step of identifying one or more glycopeptides, for example, using mass spectrometry.

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