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1. 发明申请

WO2014120647A1 SIGNAL RATIO IN ASSAY CALIBRATORS 审中-公开
标题翻译：测量校准器中的信号比率
公开(公告)号：WO2014120647A1
公开(公告)日：2014-08-07
申请号：PCT/US2014/013305
申请日：2014-01-28
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC.
发明人： SINGH, Pratap , LI, Jie , JANZEN, Roland , ZOLOTARJOVA, Nina
IPC分类号： G01N33/53
CPC分类号： G01N33/82 , G01N33/5306 , G01N33/6854
摘要： Methods of enhancing signal ratio between calibrators in an assay for an analyte include conducting an assay for the analyte with zero concentration of analyte in a first calibrator to determine a first signal level. The reagents employed in the assay comprise an antibody reagent comprising an antibody for the analyte wherein a hinge region of the antibody is conjugated to a moiety. The assay for the analyte is also conducted with a second concentration of analyte in a second calibrator to determine a second signal level wherein the second analyte concentration is greater than zero and wherein the reagents employed in the assay comprise the antibody reagent. A ratio of the first signal level to the second signal level is determined and evaluated.
摘要翻译：在分析物测定中增强校准器之间的信号比率的方法包括在第一校准器中对零分析物进行分析物测定以确定第一信号水平。在测定中使用的试剂包含抗体试剂，其包含用于分析物的抗体，其中抗体的铰链区域与部分缀合。分析物的测定还用第二校准器中的第二浓度的分析物进行，以确定第二分析物浓度大于零的第二信号水平，并且其中测定中使用的试剂包含抗体试剂。确定并评估第一信号电平与第二信号电平的比率。

2. 发明申请

WO2013096528A1 COATED SUBSTRATES FOR HIGH ENERGY CAPTURE PHASE BINDING AND METHODS OF PRODUCTION AND USE THEREOF 审中-公开
标题翻译：用于高能量捕获相结合的涂覆基材及其生产和使用方法
公开(公告)号：WO2013096528A1
公开(公告)日：2013-06-27
申请号：PCT/US2012/070763
申请日：2012-12-20
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC. , PUGIA, Michael, J. , SINGH, Pratap
发明人： PUGIA, Michael, J. , SINGH, Pratap
IPC分类号： G06F19/20 , C40B60/00 , C40B60/12 , B05D3/00
CPC分类号： G01N33/54306 , B01J19/0046 , B01J2219/00628 , B01J2219/0063 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , C40B60/12 , G01N33/54393
摘要： A substrate, which is useful for performing biological, chemical and diagnostic assays, and a method for preparing the substrate are provided. The substrate has an upper surface with a coating disposed thereon. The coating comprises a charged polymer, a non- ionic polyether, and a silicate compound. The substrate can increase capture phase binding and reduce non-specific binding of biomolecules for a biological microarray.
摘要翻译：提供了可用于进行生物，化学和诊断测定的底物和制备基质的方法。基板具有设置在其上的涂层的上表面。涂层包含带电聚合物，非离子聚醚和硅酸盐化合物。底物可以增加捕获相结合并降低生物微阵列的生物分子的非特异性结合。

3. 发明申请

WO2010104683A1 IMMUNOASSAYS EMPLOYING NON-PARTICULATE CHEMILUMINESCENT REAGENT 审中-公开
标题翻译：免费使用非颗粒性发光试剂
公开(公告)号：WO2010104683A1
公开(公告)日：2010-09-16
申请号：PCT/US2010/025433
申请日：2010-02-25
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC. , SINGH, Pratap , ZHENG, Yi, Feng , GENG, Liping , JANZEN, Roland , SCHELP, Carsten
发明人： SINGH, Pratap , ZHENG, Yi, Feng , GENG, Liping , JANZEN, Roland , SCHELP, Carsten
IPC分类号： G01N33/50
CPC分类号： G01N33/78 , G01N33/582 , G01N2333/59
摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.
摘要翻译：公开了用于进行测定的方法和试剂。本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。该组合经受用于将分析物与分析物的结合配偶体结合的条件。敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

4. 发明申请

WO2009146166A2 BIOTIN-RECEPTOR REAGENTS FOR SENSITIVITY MODULATION IN ASSAYS 审中-公开
标题翻译：用于测定灵敏度调节的生物受体试剂
公开(公告)号：WO2009146166A2
公开(公告)日：2009-12-03
申请号：PCT/US2009/040465
申请日：2009-04-14
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC. , LEWISCH, Sandra, A. , SINGH, Pratap , DESAI, Viral , KRAKOWSKI, Karen, L. , DUFFY, James, E.
发明人： LEWISCH, Sandra, A. , SINGH, Pratap , DESAI, Viral , KRAKOWSKI, Karen, L. , DUFFY, James, E.
IPC分类号： G01N33/536 , G01N33/566
CPC分类号： G01N33/532 , G01N33/54393 , G01N33/566
摘要： Methods are disclosed for designing an antibody reagent for use in an assay for the detection of an analyte to obtain an optimum assay sensitivity and/or dynamic range. The antibody reagent is a conjugate of a small molecule attached by a spacer group to an antibody for the analyte. The method comprises controlling, in the preparation of the conjugate, reaction parameters comprising the hydrophobicity or hydrophiHcity of the spacer group, the length of the spacer group, the number of molecules of the small molecule attached to the antibody and the point of attachment of the small molecule to the antibody to obtain an optimum assay sensitivity and/or dynamic range. In some embodiments the method comprises preparing two or more conjugates by selecting a set of parameters for each conjugate wherein the set of parameters is different for each conjugate, conducting an assay for the analyte employing each conjugate and selecting for use in the assay the conjugate that provides the optimum assay sensitivity and/or dynamic range.
摘要翻译：公开了设计用于分析物检测以获得最佳测定灵敏度和/或动态范围的测定中的抗体试剂的方法。抗体试剂是通过间隔基团与分析物的抗体连接的小分子的缀合物。该方法包括在共轭物的制备中控制包含间隔基团的疏水性或亲水性，间隔基团的长度，与抗体连接的小分子的分子数目和小分子到抗体以获得最佳测定灵敏度和/或动态范围。在一些实施方案中，该方法包括通过为每个缀合物选择一组参数来制备两种或更多种缀合物，其中该组参数对于每个缀合物是不同的，使用每个缀合物进行分析物的测定，并选择用于测定缀合物提供最佳测定灵敏度和/或动态范围。

5. 发明申请

WO2021030166A1 ANTI-HOG TCN1 MONOCLONAL ANTIBODIES AND METHODS OF PRODUCTION AND USE THEREOF 审中-公开
公开(公告)号：WO2021030166A1
公开(公告)日：2021-02-18
申请号：PCT/US2020/045318
申请日：2020-08-07
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC.
发明人： SHARMA, Manoj , MAREEVA, Tatiana , SMIRNOV, Yuriy , SINGH, Pratap , BEDZYK, William
IPC分类号： A61K38/00 , A61K39/00 , A61K39/395 , C07K16/18 , C07K16/46 , C07K19/00
摘要： Anti-hog TCN1 monoclonal antibodies are disclosed, along with epitopes recognized by same. Also disclosed are kits containing the monoclonal antibodies and methods of producing the antibodies. Further disclosed are methods of using the monoclonal antibodies, such as (but not limited to) in methods of estimating and/or removing TCN1 from hog intrinsic factor preparations.

6. 发明申请

WO2013101265A1 REDUCING NON-COVALENTLY BOUND POLYSACCHARIDE ON SUPPORTS 审中-公开
标题翻译：在支持下减少非共轭多糖
公开(公告)号：WO2013101265A1
公开(公告)日：2013-07-04
申请号：PCT/US2012/025342
申请日：2012-02-16
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC. , LELE, Bhalchandra , SINGH, Pratap , CHATTERJEE, Asoke
发明人： LELE, Bhalchandra , SINGH, Pratap , CHATTERJEE, Asoke
IPC分类号： C07H1/00 , B05D3/00
CPC分类号： C08B37/0021 , C08B37/00 , C08L5/06 , C08L25/06
摘要： Methods and reagents are disclosed for reducing an amount of non-covalently bound polysaccharide on a support. The method comprises contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.
摘要翻译：公开了减少载体上非共价结合多糖的量的方法和试剂。该方法包括将包含共价结合的多糖和非共价结合的多糖的载体与包含一定量的有效从载体中除去非共价结合的多糖的离液剂的水溶液接触。

7. 发明申请

WO2011159548A1 COMPOSITION FOR USE AS AN ASSAY REAGENT 审中-公开
标题翻译：用作测定试剂的组合物
公开(公告)号：WO2011159548A1
公开(公告)日：2011-12-22
申请号：PCT/US2011/039825
申请日：2011-06-09
申请人： SIEMENS HEALTHCARE DIAGNOSTICS INC. , LELE, Bhalchandra , SINGH, Pratap , BARTZ, Raphael , KELLOGG, Jason, A.
发明人： LELE, Bhalchandra , SINGH, Pratap , BARTZ, Raphael , KELLOGG, Jason, A.
IPC分类号： A61K9/00
CPC分类号： C09D133/26 , C08F120/60 , C08F126/10 , C08F220/06 , C08F220/26 , C08F220/38 , C08F220/58 , C08F2800/20 , C09D139/06 , C09D177/06 , C09D179/04 , C09D181/00 , G01N33/533 , G01N33/582
摘要： A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.
摘要翻译：用作测定试剂的组合物包括固体支持物，其包括信号产生系统的成员和合成共聚物的涂层。合成共聚物包含第一聚合单体，其包括包含反应性官能团或反应性官能团的衍生物的侧基部分和包含至少包含1个碳原子和至少2个杂原子的侧链部分的第二聚合单体。在一些实施方案中，共聚物包含包含侧链部分的聚乙烯主链，其包含反应性官能团或反应性官能团的衍生物，并且所述侧链部分包含至少1个碳原子和至少2个杂原子。

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