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1. 发明申请

WO2013000544A3 BIOREACTOR CONTAINER AND INTEGRITY CHECK METHOD FOR BIOREACTOR CONTAINERS 审中-公开
标题翻译：生物反应器罐和完整性测试程序容器生物反应器
公开(公告)号：WO2013000544A3
公开(公告)日：2013-04-04
申请号：PCT/EP2012002541
申请日：2012-06-15
申请人： SARTORIUS STEDIM BIOTECH GMBH , DAHLBERG MARTIN , GAY ISABELLE , BOETTCHER LARS , OBERMANN STEFAN , SANDROCK RAINER
发明人： DAHLBERG MARTIN , GAY ISABELLE , BOETTCHER LARS , OBERMANN STEFAN , SANDROCK RAINER
IPC分类号： A61J1/10 , A61J1/16 , B32B1/02 , B32B3/30 , B32B5/02 , B32B27/12 , G01M3/32
CPC分类号： C12M23/14 , A61J1/10 , A61J1/16 , B32B1/02 , B32B3/30 , B32B5/022 , B32B5/024 , B32B5/18 , B32B2307/726 , B32B2307/7265 , C12M23/02 , C12M23/26 , F04C2270/0421 , G01M3/04 , G01M3/22 , G01M3/3218 , G01M3/3272
摘要： One embodiment of the invention relates to a bioreactor container which comprises: walls that are flexible at least in sections and at least one container opening, the walls of the bioreactor container having a fluid-tight inner layer and the walls of the bioreactor container having an outer layer that is permeable to fluids or structured in at least sections thereof. The invention further relates to a method for checking the integrity of the bioreactor container.
摘要翻译：本发明的一个方面涉及一种生物反应器容器，包括： - 至少部分柔性的壁，以及 - 至少一个容器开口，其特征在于，所述生物反应器容器的壁具有流体密封的内层和所述生物反应器容器的壁具有至少部分流体可渗透的或结构化的外层，以及用于测试的方法生物反应容器的完整性。

2. 发明申请

WO2009106248A3 INCUBATOR COMPRISING A SHAKING DEVICE 审中-公开
标题翻译：孵化设备
公开(公告)号：WO2009106248A3
公开(公告)日：2009-11-05
申请号：PCT/EP2009001089
申请日：2009-02-17
申请人： SARTORIUS STEDIM BIOTECH GMBH , BAUMFALK REINHARD , OBERMANN STEFAN , SANDROCK RAINER
发明人： BAUMFALK REINHARD , OBERMANN STEFAN , SANDROCK RAINER
IPC分类号： C12M1/00 , C12M1/02
CPC分类号： C12M23/50 , B01F11/00 , B01F11/0008 , B01F11/0014 , B01F11/0094 , C12M23/48 , C12M27/00 , C12M27/16 , C12M41/14 , Y10S435/809
摘要： Disclosed is an incubator (1) comprising an incubation chamber (2), a shaking device (4) which can be driven by a drive unit and is used for receptacles (5) that can be placed in the incubation chamber (2) and contain cell cultures, and a device compartment (3) which adjoins the incubation chamber (2) and is used for accommodating at least parts of the shaking device (4) that projects into the incubation chamber (2). The shaking device (4) encompasses a base plate (8) which seals the incubation chamber (2) relative to the device compartment (4). On the internal side (9) facing the incubation chamber (2), the base plate (8) is equipped with a shaking table (11) that can be moved on a horizontal plane. On the external side (37) of the base plate (8) facing away from the internal side (9), a motor (34) is arranged which drives a drive shaft (14) that is rotatably mounted within the base plate (8) and is effectively connected to the driving arm (10).
摘要翻译：恒温箱（1）的温育室（2），对于在温育摇可驱动由驱动振动器（4）（2）anordnebaren容器（5）与细胞培养物，并在温育（2）相邻的设备空间（3），用于在孵育室的至少一部分接收突出（2）摇动器（4），其中，所述振荡器（4）包括基板（8），其是在温育（2）相对于所述装置的空间（4）密封，并在其温育（2 有）的面向内（9）A（在水平平面中移动振动台（11的驱动臂10）），上（从底板（8）的外侧（37）背向内侧9），电动机（34）被布置，通过该驱动臂可旋转地安装在驱动轴（14）的底板（8）中的驱动臂（10）可操作地与驱动臂（10）操作连接。

3. 发明申请

WO2007131596A8 METHOD FOR DETERMINING THE VIABILITY OF CELLS IN CELL CULTURES 审中-公开
标题翻译：法确定细胞活力细胞培养
公开(公告)号：WO2007131596A8
公开(公告)日：2008-10-02
申请号：PCT/EP2007003568
申请日：2007-04-24
申请人： SARTORIUS STEDIM BIOTECH GMBH , OBERMANN STEFAN , BAUMFALK REINHARD , REIF OSCAR-WERNER , WURM FLORIAN , DE JESUS MARIA , JORDAN MARTIN , STETTLER MATTHIEU
发明人： OBERMANN STEFAN , BAUMFALK REINHARD , REIF OSCAR-WERNER , WURM FLORIAN , DE JESUS MARIA , JORDAN MARTIN , STETTLER MATTHIEU
IPC分类号： G01N15/04 , G01N33/50 , G01N33/58
CPC分类号： G01N33/58 , G01N1/30 , G01N15/042 , G01N33/5005 , Y10T436/25375
摘要： The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: colouring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are coloured to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the coloured cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently coloured sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.
摘要翻译：本发明涉及一种用于确定在细胞培养物的细胞的活力，其包括以下步骤的方法：染色用染料，其能够穿透，这取决于该个体的细胞的生存力，确定所述比例不同程度的染色的细胞的样品。本发明的特征在于，所述着色细胞作为样品的在样品容器中的细胞悬浮液（10）进行离心分离，直至存在于样品固体部分作为压缩细胞饼（13）已经稳定，以及不同的卷染色分数判定压实的细胞滤饼（13）的部分（131，132，133）被确定为对于不同的存活力细胞的比例的量度。

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