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1. 发明申请

WO2007131596A8 METHOD FOR DETERMINING THE VIABILITY OF CELLS IN CELL CULTURES 审中-公开
标题翻译：法确定细胞活力细胞培养
公开(公告)号：WO2007131596A8
公开(公告)日：2008-10-02
申请号：PCT/EP2007003568
申请日：2007-04-24
申请人： SARTORIUS STEDIM BIOTECH GMBH , OBERMANN STEFAN , BAUMFALK REINHARD , REIF OSCAR-WERNER , WURM FLORIAN , DE JESUS MARIA , JORDAN MARTIN , STETTLER MATTHIEU
发明人： OBERMANN STEFAN , BAUMFALK REINHARD , REIF OSCAR-WERNER , WURM FLORIAN , DE JESUS MARIA , JORDAN MARTIN , STETTLER MATTHIEU
IPC分类号： G01N15/04 , G01N33/50 , G01N33/58
CPC分类号： G01N33/58 , G01N1/30 , G01N15/042 , G01N33/5005 , Y10T436/25375
摘要： The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: colouring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are coloured to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the coloured cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently coloured sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.
摘要翻译：本发明涉及一种用于确定在细胞培养物的细胞的活力，其包括以下步骤的方法：染色用染料，其能够穿透，这取决于该个体的细胞的生存力，确定所述比例不同程度的染色的细胞的样品。本发明的特征在于，所述着色细胞作为样品的在样品容器中的细胞悬浮液（10）进行离心分离，直至存在于样品固体部分作为压缩细胞饼（13）已经稳定，以及不同的卷染色分数判定压实的细胞滤饼（13）的部分（131，132，133）被确定为对于不同的存活力细胞的比例的量度。

2. 发明申请

WO2010052002A8 DOSING SYSTEM AND METHOD FOR THE DOSING OF A MEDIUM 审中-公开
标题翻译：给药系统和给药方法
公开(公告)号：WO2010052002A8
公开(公告)日：2010-08-05
申请号：PCT/EP2009007940
申请日：2009-11-05
申请人： SARTORIUS STEDIM BIOTECH GMBH , SARTORIUS AG , REIF OSCAR-WERNER , BAUMFALK REINHARD , SCHMIDT DIETER , OLDENDORF CHRISTIAN , WEITEMEIER SWEN
发明人： REIF OSCAR-WERNER , BAUMFALK REINHARD , SCHMIDT DIETER , OLDENDORF CHRISTIAN , WEITEMEIER SWEN
IPC分类号： B01F13/10 , B01F15/04
CPC分类号： B01F13/1055 , B01F15/0445
摘要： The present invention relates to a dosing system and method for the dosing of a medium, having a dosing controller (4), at least one container (2) which can be exchanged and which contains the medium for dosing, and having a dosing valve (3, 3') which can be connected to the container (2). At least one part of the dosing valve (3, 3') which comes into contact with the medium is designed as an exchangeable system component (7, 10), and also has a coding which can be read by a machine and which serves for identification. The at least one container (2), as an exchangeable system component, has a coding which can be read by a machine and which serves for identification, and the exchangeable parts (2, 7, 10, 19) that come into contact with the medium are designed as single-use parts. The dosing controller (4) is connected to a communication unit (5) for reading the coding, and the communication unit (5) has a unique reading connection (22) to each exchangeable system component (2, 7, 10, 19).
摘要翻译：计量系统和方法，用于定量给料的介质与计量控制单元（4），含有介质的至少一个要被匀称可互换容器（2）和连接到所述容器（2）的计量阀（3,3“），至少一个与所述介质接触所述计量阀（3,3“）的传入一部分形成一可更换的系统组件（7,10），并具有用于识别的机器可读代码，所述至少一个容器（2）为可更换系统部件（2）用于识别的机器可读编码其中，所述进入与介质可交换部件接触（2,7，10,19）被设计为一次性部件，并且其中所述剂量控制单元（4）用的通信单元（5）连接，用于读取码，并且其中，所述通信单元（5 ）到每个可更换系统组件（2,7,10,19）或它们的编码具有其自己的读取连接（22）。

3. 发明申请

WO2009106248A3 INCUBATOR COMPRISING A SHAKING DEVICE 审中-公开
标题翻译：孵化设备
公开(公告)号：WO2009106248A3
公开(公告)日：2009-11-05
申请号：PCT/EP2009001089
申请日：2009-02-17
申请人： SARTORIUS STEDIM BIOTECH GMBH , BAUMFALK REINHARD , OBERMANN STEFAN , SANDROCK RAINER
发明人： BAUMFALK REINHARD , OBERMANN STEFAN , SANDROCK RAINER
IPC分类号： C12M1/00 , C12M1/02
CPC分类号： C12M23/50 , B01F11/00 , B01F11/0008 , B01F11/0014 , B01F11/0094 , C12M23/48 , C12M27/00 , C12M27/16 , C12M41/14 , Y10S435/809
摘要： Disclosed is an incubator (1) comprising an incubation chamber (2), a shaking device (4) which can be driven by a drive unit and is used for receptacles (5) that can be placed in the incubation chamber (2) and contain cell cultures, and a device compartment (3) which adjoins the incubation chamber (2) and is used for accommodating at least parts of the shaking device (4) that projects into the incubation chamber (2). The shaking device (4) encompasses a base plate (8) which seals the incubation chamber (2) relative to the device compartment (4). On the internal side (9) facing the incubation chamber (2), the base plate (8) is equipped with a shaking table (11) that can be moved on a horizontal plane. On the external side (37) of the base plate (8) facing away from the internal side (9), a motor (34) is arranged which drives a drive shaft (14) that is rotatably mounted within the base plate (8) and is effectively connected to the driving arm (10).
摘要翻译：恒温箱（1）的温育室（2），对于在温育摇可驱动由驱动振动器（4）（2）anordnebaren容器（5）与细胞培养物，并在温育（2）相邻的设备空间（3），用于在孵育室的至少一部分接收突出（2）摇动器（4），其中，所述振荡器（4）包括基板（8），其是在温育（2）相对于所述装置的空间（4）密封，并在其温育（2 有）的面向内（9）A（在水平平面中移动振动台（11的驱动臂10）），上（从底板（8）的外侧（37）背向内侧9），电动机（34）被布置，通过该驱动臂可旋转地安装在驱动轴（14）的底板（8）中的驱动臂（10）可操作地与驱动臂（10）操作连接。

4. 发明申请

WO2010145735A2 CONTAINER WITH A SENSOR ADAPTER 审中-公开
标题翻译：用传感器器容器
公开(公告)号：WO2010145735A2
公开(公告)日：2010-12-23
申请号：PCT/EP2010002808
申请日：2010-05-07
申请人： SARTORIUS STEDIM BIOTECH GMBH , BAUMFALK REINHARD , KAHLERT WOLFGANG , GRELLER GERHARD
发明人： BAUMFALK REINHARD , KAHLERT WOLFGANG , GRELLER GERHARD
IPC分类号： G01D11/30
CPC分类号： G01D11/30 , C21C5/527 , C21C5/565 , C22B1/005 , C22B7/006 , C22B19/30 , Y02P10/216 , Y02P10/234
摘要： The invention relates to a container with a sensor adapter for accommodating a sensor arrangement for measuring at least one parameter of media contained in an inner space of the container, wherein the sensor adapter is arranged at the wall to the inner space of the container and comprises a receiving opening, which is accessible from the outside, of a receiving channel, the boundaries of which facing the inner space of the container for adapting the sensor arrangement. According to the invention, the receiving channel of the sensor adapter comprises towards the inner space of the container at least one boundary surface formed by a membrane, by which the medium or media to be measured are supplied to the sensor arrangement.
摘要翻译：容器与传感器适配器，用于接收一个传感器装置，用于测量包含在容器内部的媒体，其中所述传感器适配器被布置在壁到所述容器内部的至少一个参数，并朝向内部容器的外部可访问的接收开口有界接收信道，用于适配所述传感器装置，其中，所述朝向至少接收传感器适配器的信道向容器内部通过，通过该传感器装置，或者介质将被测量可以被供给的膜形成的边界表面。

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