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1. 发明申请

WO0116149A2 2-AZAPURINE COMPOUNDS AND THEIR USE 审中-公开
标题翻译： 2-AZAPURINE化合物及其用途
公开(公告)号：WO0116149A2
公开(公告)日：2001-03-08
申请号：PCT/EP0008371
申请日：2000-08-28
申请人： ROCHE DIAGNOSTICS GMBH , SEELA FRANK , ROSEMEYER HELMUT , SCHWEINBERGER ENNO , HEINDL DIETER , BERGMANN FRANK
发明人： SEELA FRANK , ROSEMEYER HELMUT , SCHWEINBERGER ENNO , HEINDL DIETER , BERGMANN FRANK
IPC分类号： G01N33/53 , C07H19/04 , C07H19/10 , C07H19/20 , C07H21/00 , C12N15/09 , C12N15/113 , C12P19/34 , C12Q1/08 , C12Q1/68 , G01N33/566 , C07H19/00
CPC分类号： C12N15/113 , C07H19/04 , C07H19/10 , C07H19/20 , C07H21/00 , C12N2310/333 , C12Q1/6813
摘要： Within oligonucleotides 2-azapurine and especially 2-azaadenine bases form specifically base pairs with guanine. This base pair is of analogous stability as an adenine-thymine but less stable than a guanine-cytosine base pair. Therefore, the incorporation of 2-azaadenine residues into oligonucleotides instead of cytosine leads specifically to hybridization complexes with nucleic acids with homogenous stability. This is useful for the adaptation of the stabilities of different oligonucleotide sequences in all kinds of hybridization techniques, for example in oligomer chip technology.
摘要翻译：寡核苷酸2-氮杂嘌呤，尤其是2-氮杂腺嘌呤碱基与鸟嘌呤特异性碱基对形成。该碱基对具有与腺嘌呤 - 胸腺嘧啶类似的稳定性，但不如鸟嘌呤 - 胞嘧啶碱基对稳定。因此，将2-氮杂腺嘌呤残基并入寡核苷酸而不是胞嘧啶，特别导致与具有均匀稳定性的核酸的杂交复合物。这对于在各种杂交技术中适应不同寡核苷酸序列的稳定性是有用的，例如在低聚物芯片技术中。

2. 发明申请

WO2013113822A3 OZONE SCAVENGERS FOR NUCLEIC ACID HYBRIDIZATION EXPERIMENTS 审中-公开
标题翻译：臭氧清除剂用于核酸杂交实验
公开(公告)号：WO2013113822A3
公开(公告)日：2013-10-10
申请号：PCT/EP2013051915
申请日：2013-01-31
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , BERGMANN FRANK , HEINDL DIETER , JOSEL HANS-PETER , LUCKEY JOHN
发明人： BERGMANN FRANK , HEINDL DIETER , JOSEL HANS-PETER , LUCKEY JOHN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6813 , C12Q2527/125 , C12Q2563/107
摘要： The present invention relates to a method, a buffer and a kit for scavenging reactive oxygen species in nucleic acid hybridization experiments.
摘要翻译：本发明涉及用于在核酸杂交实验中清除活性氧的方法，缓冲液和试剂盒。

3. 发明申请

WO2008071385A8 USE OF ACETALS FOR THE ISOLATION OF NUCLEIC ACIDS 审中-公开
标题翻译：用于分离核酸的乙缩醛的用途
公开(公告)号：WO2008071385A8
公开(公告)日：2009-04-09
申请号：PCT/EP2007010795
申请日：2007-12-11
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , DONNER HORST , BERGMANN FRANK , BUCHBERGER BERND , LASSONCZYK NINA
发明人： DONNER HORST , BERGMANN FRANK , BUCHBERGER BERND , LASSONCZYK NINA
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1013 , C12N15/1003 , C12Q1/6806 , C12Q2527/125
摘要： The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译：本发明提供了水混溶性液态环状缩醛用于将核酸吸附到固相如具有二氧化硅表面的基材上的用途。为此，本发明还提供了包含所述缩醛的组合物。公开了并要求保护纯化来自样品的核酸的方法，以及用于实施这些方法的试剂盒。通过本发明的方法纯化的核酸适用于旨在检测靶核酸的测定。

4. 发明申请

WO2008071385A2 USE OF ACETALS FOR THE ISOLATION OF NUCLEIC ACIDS 审中-公开
标题翻译：用于分离核酸的乙烯的使用
公开(公告)号：WO2008071385A2
公开(公告)日：2008-06-19
申请号：PCT/EP2007010795
申请日：2007-12-11
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , DONNER HORST , BERGMANN FRANK , BUCHBERGER BERND , LASSONCZYK NINA
发明人： DONNER HORST , BERGMANN FRANK , BUCHBERGER BERND , LASSONCZYK NINA
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1013 , C12N15/1003 , C12Q1/6806 , C12Q2527/125
摘要： The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译：本发明提供了水混溶性液体环状缩醛用于将核酸吸附到固相（例如具有二氧化硅表面的基底）的用途。为此，本发明还提供包含所述缩醛的组合物。方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。

5. 发明申请

WO2006024541A3 DNA DECONTAMINATION METHOD 审中-公开
标题翻译： DNA去除方法
公开(公告)号：WO2006024541A3
公开(公告)日：2006-06-08
申请号：PCT/EP2005009475
申请日：2005-09-02
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , BERGMANN FRANK , MARKERT-HAHN CHRISTINE
发明人： BERGMANN FRANK , MARKERT-HAHN CHRISTINE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6858 , C12Q2523/125 , C12Q2521/331
摘要： The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.
摘要翻译：本发明涉及一种用于扩增样品中的甲基化靶核酸的方法，同时避免通过使其失活来扩增非甲基化靶核酸。这是通过目标核酸亚硫酸氢盐处理后的限制酶消化完成的。本发明进一步涉及使用限制酶避免扩增未甲基化的靶核酸同时扩增样品中的甲基化靶核酸和用于实施根据本发明的方法的试剂盒。

6. 发明申请

WO9933867A3 MODIFIED NUCLEIC ACID ANALOGUES 审中-公开
标题翻译：修饰的核酸类似物
公开(公告)号：WO9933867A3
公开(公告)日：1999-09-02
申请号：PCT/EP9808317
申请日：1998-12-18
申请人： ROCHE DIAGNOSTICS GMBH , BERGMANN FRANK , ELTZ HERBERT VON DER , HERRMANN RUPERT , KOEHLER STEFANIE , SCHLUETER VOLKER
发明人： BERGMANN FRANK , VON DER ELTZ HERBERT , HERRMANN RUPERT , KOEHLER STEFANIE , SCHLUETER VOLKER
IPC分类号： C07H21/00 , C07K14/00 , C12Q1/68
CPC分类号： C07K14/003 , C07H21/00 , C12Q1/6832 , Y02P20/55 , C12Q2563/173 , C12Q2527/119 , C12Q2525/113
摘要： Oligomers comprising at least one non-nucleoside unit containing an acid function with a pKa that is lower than 3.0 are particularly suitable as detection probes for nucleic acids as non-specific interactions are reduced in comparison with unmodified oligomers. Even when the concentration of hybridisation buffers is low, it is possible to obtain high signal dynamics and high sensitivity for a low blank signal. Said oligomers are also suitable as primers for lengthening reactions using polymerases and for transfection.
摘要翻译：具有包含具有小于3.0的pKa的酸功能的至少一个非核苷单元的低聚物，特别适合作为检测探针对核酸，因为非特异性相互作用比较了与未修饰的低聚物减小，特别是在杂交的低盐浓度缓冲液在高信号的动态和灵敏度可以在低空白信号下实现。另外，它们也适合用作使用聚合酶进行延伸反应和用于转染的引物。

7. 发明申请

WO2014019707A2 NEW IRIDIUM-BASED COMPLEXES FOR ECL 审中-公开
标题翻译：用于ECL的新的铱基配合物
公开(公告)号：WO2014019707A2
公开(公告)日：2014-02-06
申请号：PCT/EP2013002321
申请日：2013-08-02
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , UNIV MUENSTER WILHELMS , ROCHE DIAGNOSTICS OPERATIONS
发明人： BERGMANN FRANK , CYSEWSKI ROBERT , DE COLA LUISA , DZIADEK SEBASTIAN , FERNANDEZ HERNANDEZ JESUS MIGUEL , JOSEL HANS-PETER , SEIDEL CHRISTOPH
IPC分类号： C07F15/00 , C09K11/06
CPC分类号： C07F15/0033 , C09K11/06 , C09K2211/1007 , C09K2211/1011 , C09K2211/1029 , C09K2211/14 , C09K2211/1408 , C09K2211/1416 , C09K2211/1425 , C09K2211/1441 , C09K2211/1466 , C09K2211/185 , G01N33/582
摘要： The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte.
摘要翻译：本发明涉及新颖的基于铱的Ir（III）发光复合物，包含这些复合物作为标记的缀合物及其应用，例如，在基于电化学发光的分析物检测中。

8. 发明申请

WO2014019709A3 NEW IRIDIUM-BASED COMPLEXES FOR ECL 审中-公开
公开(公告)号：WO2014019709A3
公开(公告)日：2014-04-03
申请号：PCT/EP2013002323
申请日：2013-08-02
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , UNIV MUENSTER WILHELMS , ROCHE DIAGNOSTICS OPERATIONS
发明人： BERGMANN FRANK , CYSEWSKI ROBERT , DE COLA LUISA , DZIADEK SEBASTIAN , FERNANDEZ HERNANDEZ JESUS MIGUEL , JOSEL HANS-PETER , SEIDEL CHRISTOPH
IPC分类号： C09K11/06 , C07F15/00
CPC分类号： C07F15/0033 , C09K11/06 , C09K11/07 , C09K2211/1007 , C09K2211/1029 , C09K2211/14 , C09K2211/1408 , C09K2211/1416 , C09K2211/1425 , C09K2211/1441 , C09K2211/1466 , C09K2211/185 , G01N33/582
摘要： The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte.

9. 发明申请

WO2008064667A8 METHOD FOR THE PRODUCTION OF ELEMENTAL MERCURY FROM MERCURY COMPOUNDS 审中-公开
标题翻译：从汞化合物生产元素汞的方法
公开(公告)号：WO2008064667A8
公开(公告)日：2008-09-04
申请号：PCT/DE2007002165
申请日：2007-11-30
申请人： UNIV HALLE WITTENBERG , KANEFKE RICO , KOESER HEINZ , VOSTEEN BERNHARD , FEHRE KRISTINA , BERGMANN FRANK , STOLLE RAIK
发明人： KANEFKE RICO , KOESER HEINZ , VOSTEEN BERNHARD , FEHRE KRISTINA , BERGMANN FRANK , STOLLE RAIK
IPC分类号： G01N33/00 , G01N31/10
CPC分类号： G01N33/0045 , G01N33/0013
摘要： The aim of the invention is to develop a method allowing mercury to be determined in exhaust gases. All previously known methods for processing exhaust gases use wet chemical processes or thermocatalytic reactors, resulting in not inconsiderable secondary reactions of the components contained in the exhaust gases in addition to mercury. The aim of the invention is to develop a method which reliably converts Hg compounds into measurable Hg(el) without causing said secondary reactions in the presence of other flue gas components. Said aim is achieved by a low-temperature method that uses copper or copper alloys as a catalyst material for converting mercury compounds into elemental mercury. Red brass RG7 is particularly suitable, having the advantageous property of binding free halogens and thus preventing back reaction into mercury halides. Because of the low temperatures, said method prevents SO 2 from being converted into SO 3 while the metal surfaces of the measuring instrument are not additionally corroded. The test gas flows through an Hg converter in which the oxidized Hg compounds (e.g. HgCl 2 ) are converted into elemental Hg with the help of the copper and other metals (Sn, Zn, Pb) contained in the red brass. The invention is used for determining exhaust gases in coal-fired power plants, waste incinerators, and cement factories.
摘要翻译：本发明的目的是开发一种能够测定废气中的汞的方法。所有已知的用于处理废气用的所谓湿化学或热催化反应器工作，导致由废气中添加汞的不可忽视的副反应的方法进一步包含的成分。其目的是开发一种将汞化合物可靠转化为可测量汞（el）的工艺，该工艺在其他烟气成分的存在下不会产生上述副反应。使用低温工艺，其使用铜或铜合金作为将汞化合物转化为元素汞的催化剂材料。特别合适的是铜合金炮铜RG7。这种材料具有结合游离卤素的有利性质并因此防止了对汞卤化物的逆反应。该方法不会导致由于低温下SO _{2 的转化在SO _{3 ，不会发生所述测量装置的金属表面上的附加攻击。样品气体流过汞转换器，其中氧化的汞化合物（如氯化汞_{2 ）。随着铜的助剂（以及其它包含在红黄铜的金属：锡，锌，铅）的元素汞被转化。测定燃煤电厂，垃圾焚烧厂，水泥厂的废气。}}}

10. 发明申请

WO0248274A3 FLEXIBLE AND VISCOPLASTIC METHACRYLATE ADHESIVES 审中-公开
标题翻译：柔韧和弹性的甲基丙烯酸酯粘合剂
公开(公告)号：WO0248274A3
公开(公告)日：2002-09-19
申请号：PCT/EP0114374
申请日：2001-12-07
申请人： HENKEL KGAA , NICOLAISEN HEINZ-CHRISTIAN , BERGMANN FRANK , RENZ ANDREAS , HUVER THOMAS
发明人： NICOLAISEN HEINZ-CHRISTIAN , BERGMANN FRANK , RENZ ANDREAS , HUVER THOMAS
IPC分类号： C09J4/00 , C09J4/06 , C09J121/00 , C09J175/00
CPC分类号： C09J4/06 , C08F290/147 , C09J4/00 , C08F220/28 , C08F279/00
摘要： The invention relates to a single or dual component adhesive compositions based on at least one (meth)acrylate monomer, at least one urethane (meth)acrylate, in addition to dissolved rubbers, and an initiator / activator system which can be used according to anaerobic or aerobic hardening mechanisms as single component adhesives, and used as dual component adhesives in the so-called A-B method. In comparison to known adhesives of said type, the inventive adhesives exhibit high flexibility and viscoplasticity, and are characterised by high tensile strength and a high T-peel resistance.
摘要翻译： 1分别。的至少一种（甲基）arcylatmonomeren，至少一个氨基甲酸酯的基础上的双组分粘合剂组合物 - （甲基）丙烯酸酯，和溶解在其中的橡胶，以及一种引发剂/活化剂体系可用于根据比1组分厌氧或需氧固化机理粘合剂，和作为根据所谓的AB方法使用的双组分粘合剂。与这种类型的先前已知的粘合剂相比，它们具有高的柔软性和韧性和弹性并且通过霍霍埃剥离强度它们的高拉伸剪切强度的特征。

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