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1. 发明申请

WO2014165198A1 GENERATION OF pH/TEMPERATURE/IONIC GRADIENTS IN LATERAL FLOW IMMUNOASSAYS FOR MODULATING BIOMOLECULAR INTERACTIONS AND ITS APPLICATIONS 审中-公开
标题翻译：用于调节生物分子相互作用的流动免疫测定中pH /温度/离子梯度的产生及其应用
公开(公告)号：WO2014165198A1
公开(公告)日：2014-10-09
申请号：PCT/US2014/024760
申请日：2014-03-12
申请人： ROBERT BOSCH GMBH , JOHNSON, Christopher , KAVUSI, Sam , AHMAD, Habib , LANG, Christoph
发明人： JOHNSON, Christopher , KAVUSI, Sam , AHMAD, Habib , LANG, Christoph
IPC分类号： B01L3/00 , G01N33/50
CPC分类号： G01N27/3276 , B01L3/5027 , B01L2300/0636 , B01L2300/0645 , B01L2300/069 , B01L2300/089 , B01L2400/0406 , G01N33/5438 , G01N33/558
摘要： Lateral flow assay devices comprising a multisite array of test sites (8) for measuring a biomolecule analyte (3) having a solid support (5) including absorbent material for providing capillary flow, one device being divided into parallel lanes (7) in the flow direction. The devices comprising a sample portion for receiving a sample; a diagnostic portion comprising a multisite array of test sites (8) each comprising a probe for the biomolecular analyte (3), wherein at each test site of the multisite array the conditions for the interaction between the biomolecular analyte and the probe can be varied independently; an absorbent portion of absorbent material for providing capillary flow; and electrodes (10) for actuating and controlling conditions. The sample portion, diagnostic portion, and absorbent portion are in capillary flow communication resulting in a capillary flow from sample portion towards absorbent portion, whereby the sample flows across the probe at each test site in the diagnostic portion to provide contact between the sample and such probe. Methods for measuring analyte concentration in a sample using such lateral flow assay devices.
摘要翻译：包括用于测量具有固体支持物（5）的生物分子分析物（3）的测试位点（8）的多部分阵列的横向流动测定装置，所述固体支撑物包括用于提供毛细血管流动的吸收材料，一个装置被分成流动的平行通道（7）方向。所述装置包括用于接收样品的样品部分; 诊断部分包括测试部位（8）的多部分阵列，每个测试部位包括用于生物分子分析物（3）的探针，其中在多位点阵列的每个测试位置处，生物分子分析物和探针之间的相互作用条件可以独立变化 ; 用于提供毛细管流的吸收材料的吸收部分; 以及用于致动和控制条件的电极（10）。样品部分，诊断部分和吸收部分处于毛细管流动连通，导致从样品部分向吸收部分的毛细管流动，由此样品在诊断部分的每个测试部位处流过探针，以提供样品与其间的接触探测。使用这种侧流测定装置测量样品中分析物浓度的方法。

2. 发明申请

WO2021122054A1 NANOSCALE TOPOGRAPHY SYSTEM FOR USE IN DNA SEQUENCING AND METHOD FOR FABRICATION THEREOF 审中-公开
公开(公告)号：WO2021122054A1
公开(公告)日：2021-06-24
申请号：PCT/EP2020/084623
申请日：2020-12-04
申请人： ROBERT BOSCH GMBH
发明人： FOMINA, Nadezda , BUCCI, Giovanna , JOHNSON, Christopher , CRAIG, Nathan P. , SHIN, Young Shik , GADELRAB, Karim
IPC分类号： B01L3/00 , B01L2200/0663 , B01L2300/0645 , B01L2300/0663 , B01L2300/0816 , B01L2300/0864 , B01L2300/0896 , B01L2400/086 , B01L3/502746 , B01L3/502761 , B82B1/001 , B82B3/0019 , C12Q1/6806 , G01N1/286
摘要： A method of fabricating a nanoscale topography system for inducing unfolding of a DNA molecule for sequencing includes providing a substrate and creating trench walls on the substrate which define a trench therebetween. The method further includes depositing a layer of a block copolymer (BCP) in the trench and forming cylindrical domains by self-assembly of the BCP between the trench walls, removing a first portion of the cylindrical domains to create a vacant region in the trench, and depositing a subsequent layer of the BCP in the vacant region and forming spherical domains by self-assembly of the BCP between the trench walls adjacent a second portion of the cylindrical domains. The spherical domains form staggered post structures for unfolding the DNA molecule and the cylindrical domains form parallel channel structures for entry of the DNA molecule for sequencing.

3. 发明申请

WO2020141350A1 PH-MODULATED IMAGING OF TARGETS CLOSE TO A SOLID SURFACE 审中-公开
公开(公告)号：WO2020141350A1
公开(公告)日：2020-07-09
申请号：PCT/IB2019/001438
申请日：2019-12-17
申请人： ROBERT BOSCH GMBH
发明人： SHIN, Young Shik , STALEY, Patrick A. , FOMINA, Nadia , JOHNSON, Christopher
IPC分类号： G01N21/17 , G01N27/327 , G01N33/53
摘要： Provided are methods for imaging a biospecimen, which involves the use of electrochemical pH modulation in combination with pH-sensitive labels to achieve localized imaging with a high vertical axial resolution.

4. 发明申请

WO2017005586A1 ELECTRONIC CONTROL OF THE PH OF A SOLUTION CLOSE TO AN ELECTRODE SURFACES 审中-公开
标题翻译：电解液接近电极表面的电解液的电子控制
公开(公告)号：WO2017005586A1
公开(公告)日：2017-01-12
申请号：PCT/EP2016/065249
申请日：2016-06-30
申请人： ROBERT BOSCH GMBH
发明人： MARUNIAK, Autumn , AHMAD, Habib , RAGHUNATHAN, Ashwin , JOHNSON, Christopher , KAVUSI, Sam , FOMINA, Nadezda , LANG, Christoph
IPC分类号： G05D21/02 , G01N27/416 , G01N27/30
CPC分类号： G01N27/4167 , C12Q1/001 , G01N27/302 , G01N27/34 , G01N27/36 , G05D21/02
摘要： Device and methods for use in a biosensor comprising a multisite array of test sites, the device and methods being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest by modulating the pH or ionic gradient near the electrodes in such biosensor. An electrochemically active agent that is suitable for use in biological buffers for changing the pH of the biological buffers. Method for changing the pH of biological buffers using the electrochemically active agents. The methods of modulating the binding interactions provided in a biosensor, analytic methods for more accurately controlling and measuring the pH or ionic gradient near the electrodes in such biosensor, and analytic methods for more accurately measuring an analyte of interest in a biological sample.
摘要翻译：用于生物传感器的装置和方法，包括测试点的多位阵列，所述装置和方法可用于通过调节（生物分子）探针或检测剂与所分析物之间的结合相互作用，通过调节附近的pH或离子梯度这种生物传感器中的电极。适用于改变生物缓冲液pH值的生物缓冲液中的电化学活性剂。使用电化学活性剂改变生物缓冲液的pH的方法。调节生物传感器中提供的结合相互作用的方法，用于更准确地控制和测量这些生物传感器中电极附近的pH或离子梯度的分析方法，以及用于更精确地测量生物样品中感兴趣分析物的分析方法。

5. 发明申请

WO2022016087A1 ELECTRONIC CONTROL OF THE PH OF A SOLUTION CLOSE TO AN ELECTRODE SURFACE 审中-公开
公开(公告)号：WO2022016087A1
公开(公告)日：2022-01-20
申请号：PCT/US2021/042031
申请日：2021-07-16
申请人： ROBERT BOSCH GMBH , JOHNSON, Christopher , KAVUSI, Sam , FOMINA, Nadezda , AHMAD, Habib , MARUNIAK, Autumn , LANG, Christoph , RAGHUNATHAN, Ashwin , SHIN, Young Shik , DARVISH, Armin , PAPAGEORGIOU, Efthymios
发明人： JOHNSON, Christopher , KAVUSI, Sam , FOMINA, Nadezda , AHMAD, Habib , MARUNIAK, Autumn , LANG, Christoph , RAGHUNATHAN, Ashwin , SHIN, Young Shik , DARVISH, Armin , PAPAGEORGIOU, Efthymios
IPC分类号： C12Q1/00 , G01N27/30 , G01N27/34
摘要： Device and methods for controlling pH or ionic gradient comprising a multisite array of feedback electrode sets comprising electrodes and pH sensing elements. The electrodes can include a reference electrode, counter electrode, and a working electrode. The device and methods iteratively select an amount of current and/or voltage to be applied to each working electrode, apply the selected amount of current and/or voltage to each working electrode to change pH of a solution close to the working electrode, and measure the signal output of the sensing element. The multisite array can include feedback and non-feedback electrode sets.

6. 发明申请

WO2019086305A1 ELECTROCHEMICAL SEQUENCING OF DNA USING AN EDGE ELECTRODE 审中-公开
公开(公告)号：WO2019086305A1
公开(公告)日：2019-05-09
申请号：PCT/EP2018/079133
申请日：2018-10-24
申请人： ROBERT BOSCH GMBH
发明人： AHMAD, Habibullah , LAERMER, Franz , YAMA, Gary , JOHNSON, Christopher , HOFFMANN, Jochen , STALEY, Patrick , SHIN, Young Shik , FOMINA, Nadezda , LANG, Christoph
IPC分类号： G01N33/487 , C12Q1/6869
CPC分类号： G01N27/3277 , C12Q1/6825 , C12Q1/6869 , G01N27/3278 , G01N33/48721 , C12Q2563/113 , C12Q2565/607
摘要： A device to electrochemically sequence DNA (108) that includes a redox species includes at least one edge electrode (102), at least one stack of insulator material (104), and a pair of DNA translocation electrodes including a DNA translocation working electrode (106a) and a DNA translocation counter electrode (106b), where the thickness of the at least one edge electrode (102) is about 0.5 nanometers, and the thickness of the at least one stack of insulator material (104) is about 10 nanometers. Methods of electrochemically sequencing a strand of DNA are also provided.

7. 发明申请

WO2018002002A1 ELECTRODE ARRANGEMENT WITH IMPROVED ELECTRON TRANSFER RATES FOR REDOX OF MOLECULES 审中-公开
标题翻译：改进分子氧化还原电子转移速率的电极排列
公开(公告)号：WO2018002002A1
公开(公告)日：2018-01-04
申请号：PCT/EP2017/065777
申请日：2017-06-27
申请人： ROBERT BOSCH GMBH
发明人： MARUNIAK, Autumn , AHMAD, Habib , JOHNSON, Christopher , KAVUSI, Sam , FOMINA, Nadezda
IPC分类号： G01N27/327 , C12Q1/00
摘要： A coated electrode includes an electrode, a coating configured to immobilize biomolecules, and a coating configured to improve electron transfer rate. Methods of making the coated electrode are also provided. A biosensor comprises a plurality of electrodes, each electrode including the coated electrode.
摘要翻译：涂覆的电极包括电极，构造成固定生物分子的涂层和构造成改善电子传递速率的涂层。还提供了制造涂覆电极的方法。生物传感器包括多个电极，每个电极包括涂覆的电极。

8. 发明申请

WO2019014317A1 SYSTEM AND METHOD FOR SINGLE-STEP ELISA VIA LOCAL PH MODULATION 审中-公开
公开(公告)号：WO2019014317A1
公开(公告)日：2019-01-17
申请号：PCT/US2018/041591
申请日：2018-07-11
申请人： ROBERT BOSCH GMBH , SHIN, Young, Shik , FOMINA, Nadezda , JOHNSON, Christopher , LANG, Christoph , STALEY, Patrick
发明人： SHIN, Young, Shik , FOMINA, Nadezda , JOHNSON, Christopher , LANG, Christoph , STALEY, Patrick
IPC分类号： G01N33/543 , G01N33/58 , G01N27/327
摘要： A method for detecting a presence and/or a concentration of a target substance in a reagent solution using enzyme-linked immunosorbent assay (ELIS A) includes binding the target substance directly or indirectly to an electrode, and binding a detection agent directly or indirectly to the bound target substance. The method further includes modulating a pH of only a portion of the reagent solution in which the bound target substance and the bound detection agent are located using the electrode, the modulated pH of the portion of the reagent solution causing the bound detection agent to undergo a change, and detecting the change in the bound detection agent. The detected change corresponds to the presence of the target substance in the reagent solution and/or the concentration of the target substance in the reagent solution.

9. 发明申请

WO2017207298A1 AZLACTONE FUNCTIONALIZED SUBSTRATES FOR CONJUGATION OF BIOMOLECULES 审中-公开
标题翻译：用于生物分子共轭的AZLACTONE功能化基质
公开(公告)号：WO2017207298A1
公开(公告)日：2017-12-07
申请号：PCT/EP2017/062083
申请日：2017-05-19
申请人： ROBERT BOSCH GMBH
发明人： MARUNIAK, Autumn , AHMAD, Habib , JOHNSON, Christopher , KAVUSI, Sam , FOMINA, Nadezda
IPC分类号： C12N11/00 , C08L1/00 , C08L3/00 , C08L5/00 , C08L23/00 , C08L33/12 , C08L33/26 , C08L71/02 , C08L77/04 , C08L79/02
摘要： A bifunctional polymer is functionalized at one end with an azlactone end group to conjugate biomolecules of interest, and is functionalized at another end with an azide anchor group to attach the polymer to a substrate. Methods of making the bifunctional polymer are also provided. A coated substrate includes the bifunctionalized polymers on the surface of a substrate. Methods of making the coated substrate are also provided. A microarray includes a plurality of discrete regions, each region including the coated substrate.
摘要翻译：双功能聚合物在一端用吖内酯端基官能化以缀合感兴趣的生物分子，并且在另一端用叠氮基锚定基团官能化以将聚合物附着到基底上。还提供了制备双官能聚合物的方法。涂覆的基材包括在基材表面上的双官能化聚合物。还提供了制造涂覆基材的方法。微阵列包括多个分立的区域，每个区域包括涂覆的基底。

10. 发明申请

WO2017005587A1 ELECTROCHEMICALLY ACTIVE AGENTS FOR PH MODULATION IN BIOLOGICAL BUFFERS 审中-公开
标题翻译：用于生物缓冲液中PH调节的电化学活性剂
公开(公告)号：WO2017005587A1
公开(公告)日：2017-01-12
申请号：PCT/EP2016/065252
申请日：2016-06-30
申请人： ROBERT BOSCH GMBH
发明人： MARUNIAK, Autumn , AHMAD, Habib , RAGHUNATHAN, Ashwin , JOHNSON, Christopher , KAVUSI, Sam , FOMINA, Nadezda , LANG, Christoph
IPC分类号： C12Q1/00 , G01N33/543 , C07C37/00
CPC分类号： C07C49/84 , C07C37/00 , C07C37/07 , C07C39/10 , C07C39/11 , C07C41/01 , C07C41/24 , C07C41/30 , C07C43/1783 , C07C43/2055 , C07C43/225 , C07C43/23 , C07C45/42 , C07C46/06 , C07C49/82 , C12Q1/001 , C12Q1/004 , G01N27/302 , G01N27/327 , G01N27/3275 , G01N27/4163 , G01N27/4167 , G01N33/5438
摘要： Device and methods for use in a biosensor comprising a multisite array of test sites, the device and methods being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest by modulating the pH or ionic gradient near the electrodes in such biosensor. An electrochemically active agent comprising quinone derivatives that is suitable for use in biological buffers for changing the pH of the biological buffers. Method for changing the pH of biological buffers using the electrochemically active agents. The methods of modulating the binding interactions provided in a biosensor, analytic methods for more accurately controlling and measuring the pH or ionic gradient near the electrodes in such biosensor, and analytic methods for more accurately measuring an analyte of interest in a biological sample.
摘要翻译：用于生物传感器的装置和方法，包括测试点的多位阵列，所述装置和方法可用于通过调节（生物分子）探针或检测剂与所分析物之间的结合相互作用，通过调节附近的pH或离子梯度这种生物传感器中的电极。包含适于用于改变生物缓冲液的pH的生物缓冲液中的醌衍生物的电化学活性剂。使用电化学活性剂改变生物缓冲液的pH的方法。调节生物传感器中提供的结合相互作用的方法，用于更准确地控制和测量这些生物传感器中电极附近的pH或离子梯度的分析方法，以及用于更精确地测量生物样品中感兴趣分析物的分析方法。

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