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1. 发明申请

WO2022082179A2 ENGINEERED CAS ENDONUCLEASE VARIANTS FOR IMPROVED GENOME EDITING 审中-公开
公开(公告)号：WO2022082179A2
公开(公告)日：2022-04-21
申请号：PCT/US2021/071839
申请日：2021-10-13
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALARCON, Clara , GASIOR, Stephen Lawrence , HOU, Zhenglin , VAN GINKEL, Elizabeth Sommers , YOUNG, Joshua K.
IPC分类号： C12N9/22 , C12N15/11 , C12N15/82 , C12N15/85
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using novel engineered Cas endonucleases. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.

2. 发明申请

WO2016186953A1 GUIDE RNA/CAS ENDONUCLEASE SYSTEMS 审中-公开
标题翻译：指南RNA / CAS内切酶体系
公开(公告)号：WO2016186953A1
公开(公告)日：2016-11-24
申请号：PCT/US2016/032073
申请日：2016-05-12
申请人： PIONEER HI BRED INTERNATIONAL INC
发明人： CIGAN, Andrew Mark , GASIUNAS, Giedrius , KARVELIS, Tautvydas , SIKSNYS, Virginijus , YOUNG, Joshua K.
IPC分类号： C12N9/22 , C12N15/113 , C12N15/90
CPC分类号： C12N15/1093 , C12N9/22 , C12N15/102 , C12N15/1051 , C12N15/113 , C12N15/8213 , C12N15/902 , C12N2310/20 , C12N2320/10 , C12Q1/6811 , C12Q2521/301 , C12Q2525/179 , C12Q2525/191 , C12Q2535/122
摘要： Compositions and methods are provided for novel guide RNA/ Cas endonuclease systems. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri Mlc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp . SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
摘要翻译：提供了新的RNA / Cas内切核酸酶系统的组成和方法。 II型Cas9内切核酸酶系统来源于短孢杆菌（Brevibacillus soosporus），罗伊氏乳杆菌（Luterobacillus reuteri）Mlc3，罗氏假丝酵母（DSM）15814，戊糖片球菌SL4，乳酸乳杆菌JCM 14932， SCADC，双歧杆菌嗜热乳杆菌DSM 20210，Loktanella vestfoldensis，Sphingomonas sanxanigenens NX02，Epilithonimonas tenax DSM 16811，孢子体孢子虫粘球菌在本文中描述。本公开还描述了用于基因组修饰细胞基因组中的靶序列，用于基因编辑以及将感兴趣的多核苷酸插入细胞基因组的方法。

3. 发明申请

WO2009103049A2 PLANT GENOMIC DNA FLANKING SPT EVENT AND METHODS FOR IDENTIFYING SPT EVENT 审中-公开
标题翻译：植物基因组DNA定位SPT事件和识别SPT事件的方法
公开(公告)号：WO2009103049A2
公开(公告)日：2009-08-20
申请号：PCT/US2009/034212
申请日：2009-02-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , BRINK, Kent , CROWGEY, Erin , DIETRICH, Nina , HONDRED, David , YOUNG, Joshua K. , ZHONG, Cathy, Xiaoyan
发明人： BRINK, Kent , CROWGEY, Erin , DIETRICH, Nina , HONDRED, David , YOUNG, Joshua K. , ZHONG, Cathy, Xiaoyan
IPC分类号： C12Q1/68
CPC分类号： A01H5/00 , C12N15/8245 , C12N15/8289
摘要： Compositions and methods related to transgenic plants comprising seed production technology are provided. Specifically, maize plants having a E6611.32.1.38 event which confers seed production technology are provided. The plant harboring the E6611.32.1.38 event at the recited chromosomal location comprises the genomic/transgene junctions described. The plant genomic DNA flanking the integrated E6611.32.1.38 event can be used to design assays that will be specific for the E6611.32.1.38 event. The characterization of the genomic insertion site of the E6611.32.1.38 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the maize E6611.32.1.38 event are provided.
摘要翻译：提供了与包含种子生产技术的转基因植物相关的组合物和方法。具体而言，提供了赋予种子生产技术的E6611.32.1.38事件的玉米植物。在所述染色体位置处携带E6611.32.1.38事件的植物包括描述的基因组/转基因连接。集成的E6611.32.1.38事件侧翼的植物基因组DNA可用于设计特异于E6611.32.1.38事件的测定。 E6611.32.1.38事件的基因组插入位点的表征提供了增强的育种效率，并且能够使用分子标记来跟踪繁殖种群及其后代中的转基因插入物。提供了用于鉴定，检测和使用玉米E6611.32.1.38事件的各种方法和组合物。

4. 发明申请

WO2020131986A1 MULTIPLEX GENOME TARGETING 审中-公开
公开(公告)号：WO2020131986A1
公开(公告)日：2020-06-25
申请号：PCT/US2019/067032
申请日：2019-12-18
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： GAO, Huirong , YOUNG, Joshua K.
IPC分类号： C12N15/113 , C12N9/22 , C12N15/85
摘要： Methods and compositions are provided for the editing of a plurality of target polynucleotides, for example in the genome of a cell, using a Cas endonuclease guided by a plurality of discrete guide RNAs provided as a poly-guide RNA sequence, wherein each of the discrete guide RNAs are flanked by a sequence (or sequences) capable of recognition and cleavage by an endoribonuclease. Also provided compositions of an endoribonuclease, and methods of use thereof. The endoribonuclease may be obtained or derived from Streptococcus thermophilus.

5. 发明申请

WO2019217358A1 METHODS AND COMPOSITIONS FOR HOMOLOGY-DIRECTED REPAIR OF CAS ENDONUCLEASE MEDIATED DOUBLE STRAND BREAKS 审中-公开
公开(公告)号：WO2019217358A1
公开(公告)日：2019-11-14
申请号：PCT/US2019/031023
申请日：2019-05-07
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： GASIOR, Stephen Lawrence , SVITASHEV, Sergei , YOUNG, Joshua K.
IPC分类号： A61K48/00 , C12N5/10 , C12N9/22 , C12N15/09 , C12N15/11 , C12N15/82
摘要： Compositions and methods are provided for effecting homology-directed repair at the site of a double-strand-break repair in a polynucleotide. In some aspects, the frequency of HDR is improved as compared to a control method. In some aspects, the ratio of HR to NHEJ is increased. In some aspects, the percentage of HR relative to the total number of mutant reads is increased.

6. 发明申请

WO2017222773A1 NOVEL CAS SYSTEMS AND METHODS OF USE 审中-公开
标题翻译：新的CAS系统和使用方法
公开(公告)号：WO2017222773A1
公开(公告)日：2017-12-28
申请号：PCT/US2017/035425
申请日：2017-06-01
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew Mark , HOU, Zhenglin , KING, Matthew G. , LIN, Haining , YOUNG, Joshua K.
IPC分类号： C12N9/22 , C12N15/10 , C12N15/113 , C12N15/82
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/ Cas endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are Cas endonucleases comprising previously undefined nuclease domains and methods employing said Cas endonucleases for production of a guide polynucleotide/ Cas endonuclease systems, for genome editing of a nucleotide sequence in the genome of a prokaryotic or eukaryotic cell, and/or for inserting or deleting a polynucleotide of interest into or from the genome of an organism.
摘要翻译：提供组合物和方法用于基因组修饰细胞基因组中的靶序列。该方法和组合物使用引导多核苷酸/ Cas核酸内切酶系统来提供用于修饰或改变细胞或生物体基因组内的靶序列的有效系统。还提供了包含先前未确定的核酸酶结构域的Cas核酸内切酶和使用所述Cas核酸内切酶用于产生指导性多核苷酸/ Cas核酸内切酶系统，用于基因组编辑原核或真核细胞基因组中的核苷酸序列和/或用于插入或从感兴趣的生物基因组中删除感兴趣的多核苷酸。

7. 发明申请

WO2011075593A1 MAIZE EVENT DP-040416-8 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-040416-8及其检测方法
公开(公告)号：WO2011075593A1
公开(公告)日：2011-06-23
申请号：PCT/US2010/060841
申请日：2010-12-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
发明人： DIEHN, Scott , LU, Albert L. , NOWATZKI, Timothy M. , NUBEL, Douglas Stuart , REGISTER, III, James Calvin , SCELONGE, Christopher Jay , VILLANELO, M. Alejandra Pascual , YOUNG, Gregory James , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
IPC分类号： C12N15/82 , A01H1/02 , C07K14/325 , A01H5/00
CPC分类号： C12N15/8286 , A01H1/02 , Y02A40/162 , Y10T436/143333
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-040416-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-040416-8事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

8. 发明申请

WO2019217816A1 METHODS FOR THE IDENTIFICATION AND CHARACTERIZATION OF DOUBLE-STRAND BREAK SITES AND COMPOSITIONS AND USES THEREOF 审中-公开
公开(公告)号：WO2019217816A1
公开(公告)日：2019-11-14
申请号：PCT/US2019/031719
申请日：2019-05-10
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , VILNIUS UNIVERSITY
发明人： DESCHAMPS, Stephane , SIKSNYS, Virginijus , YOUNG, Joshua K. , ZAREMBA, Mindaugas
IPC分类号： C12Q1/6869 , C12N9/22 , C12Q1/6855
摘要： Methods and compositions are provided for the identification, detection, characterization, and/or utilization of double strand breaks in a target polynucleotide; the identification, detection, characterization, and/or utilization of cutting sites for double-strand-break-inducing agents; and the identification, detection, characterization, and/or utilization of double-strand-break-inducing agents.

9. 发明申请

WO2019165168A1 NOVEL CAS9 ORTHOLOGS 审中-公开
公开(公告)号：WO2019165168A1
公开(公告)日：2019-08-29
申请号：PCT/US2019/019086
申请日：2019-02-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： HOU, Zhenglin , YOUNG, Joshua K. , GASIUNAS, Giedrius , SIKSNYS, Virginijus
IPC分类号： C12N9/22 , C12N15/10 , C12N15/113 , C12N15/82
摘要： Compositions and methods are provided for novel Cas9 orthologs, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for creating a double strand break in a target polynucleotide, methods for genome modification of a target sequence under various in vivo and in vitro conditions, in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having a modified target site or altered polynucleotide of interest produced by the methods described herein.

10. 发明申请

WO2017218185A1 USE OF CPF1 ENDONUCLEASE FOR PLANT GENOME MODIFICATIONS 审中-公开
标题翻译： CPF1内切核酸酶在植物基因组修饰中的应用
公开(公告)号：WO2017218185A1
公开(公告)日：2017-12-21
申请号：PCT/US2017/035175
申请日：2017-05-31
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew Mark , DJUKANOVIC, Vesna , YOUNG, Joshua K.
IPC分类号： C12N9/22 , C12N15/10 , C12N15/82
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, for genome editing of a nucleotide sequence in the genome of a plant or plant cell, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a plant. The methods and compositions employ a guide polynucleotide/ Cpf1 endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a plant, plant cell or seed. Also provided are guide polynucleotides/Cpf1 endonucleases complexes, guide polynucleotides, plant-optimized Cpf1 endonuclease sequences, recombinant DNA constructs comprising plant-optimized Cpf1 endonucleases genes, and combinations thereof.
摘要翻译：提供组合物和方法用于基因组修饰植物或植物细胞基因组中的靶序列，用于基因组编辑植物或植物细胞基因组中的核苷酸序列，和/或用于将目的多核苷酸插入或从植物基因组中缺失。该方法和组合物使用引导多核苷酸/ Cpf1内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子的基因组内的靶序列的有效系统。还提供了指导性多核苷酸/ Cpf1内切核酸酶复合物，指导性多核苷酸，植物优化的Cpf1核酸内切酶序列，包含植物优化的Cpf1内切核酸酶基因的重组DNA构建体，以及它们的组合。

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