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1. 发明申请

WO2017155717A1 NOVEL CAS9 SYSTEMS AND METHODS OF USE 审中-公开
标题翻译：新型CAS9系统和使用方法
公开(公告)号：WO2017155717A1
公开(公告)日：2017-09-14
申请号：PCT/US2017/019640
申请日：2017-02-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew Mark , KING, Matthew G. , LIN, Haining , YOUNG, Joshua K.
IPC分类号： C12N15/113 , C12N9/22 , C12N15/82
摘要： Compositions and methods are provided for novel Cas9 systems, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having an altered target site or altered polynucleotide of interest produced by the methods described herein.
摘要翻译：为新的Cas9系统提供了组合物和方法，包括但不限于新的指导性多核苷酸/ Cas9内切核酸酶复合物，单或双引导RNA，引导RNA元件和Cas9内切核酸酶。本公开还描述了用于基因组修饰细胞基因组中的靶序列，用于基因编辑以及用于将感兴趣的多核苷酸插入细胞基因组中的方法。还提供了具有通过本文所述方法产生的改变的靶位点或改变的目的多核苷酸的核酸构建体和细胞。

2. 发明申请

WO2019023590A1 SYSTEMS AND METHODS FOR TARGETED GENOME EDITING 审中-公开
公开(公告)号：WO2019023590A1
公开(公告)日：2019-01-31
申请号：PCT/US2018/044112
申请日：2018-07-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： BAUMGARTEN, Andrew , GERKE, Justin P. , GUO, Hui , KING, Matthew G. , LIN, Haining , MEELEY, Robert B. , PETERSON-BURCH, Brooke , ZHANG, Yun
IPC分类号： G06F19/22 , C12N15/113 , C12N15/63 , C12N9/22
摘要： Systems and methods are described for designing nucleotide guides for site-specific genome editing that also minimize off-target genome edits. Systems and methods are described for using these nucleotide guides to edit specific genomic regions and minimize edits to genomic regions not intended for editing.

3. 发明申请

WO2017222773A1 NOVEL CAS SYSTEMS AND METHODS OF USE 审中-公开
标题翻译：新的CAS系统和使用方法
公开(公告)号：WO2017222773A1
公开(公告)日：2017-12-28
申请号：PCT/US2017/035425
申请日：2017-06-01
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew Mark , HOU, Zhenglin , KING, Matthew G. , LIN, Haining , YOUNG, Joshua K.
IPC分类号： C12N9/22 , C12N15/10 , C12N15/113 , C12N15/82
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/ Cas endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are Cas endonucleases comprising previously undefined nuclease domains and methods employing said Cas endonucleases for production of a guide polynucleotide/ Cas endonuclease systems, for genome editing of a nucleotide sequence in the genome of a prokaryotic or eukaryotic cell, and/or for inserting or deleting a polynucleotide of interest into or from the genome of an organism.
摘要翻译：提供组合物和方法用于基因组修饰细胞基因组中的靶序列。该方法和组合物使用引导多核苷酸/ Cas核酸内切酶系统来提供用于修饰或改变细胞或生物体基因组内的靶序列的有效系统。还提供了包含先前未确定的核酸酶结构域的Cas核酸内切酶和使用所述Cas核酸内切酶用于产生指导性多核苷酸/ Cas核酸内切酶系统，用于基因组编辑原核或真核细胞基因组中的核苷酸序列和/或用于插入或从感兴趣的生物基因组中删除感兴趣的多核苷酸。

4. 发明申请

WO2017155715A1 NOVEL CAS9 SYSTEMS AND METHODS OF USE 审中-公开
公开(公告)号：WO2017155715A1
公开(公告)日：2017-09-14
申请号：PCT/US2017/019631
申请日：2017-02-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew Mark , KING, Matthew G. , LIN, Haining , YOUNG, Joshua K.
IPC分类号： C12N15/113 , C12N9/22 , C12N15/82
CPC分类号： C12N15/113 , C12N9/22 , C12N15/8207 , C12N15/821 , C12N15/8213 , C12N2310/20
摘要： Compositions and methods are provided for novel Cas9 systems, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having an altered target site or altered polynucleotide of interest produced by the methods described herein.

5. 发明申请

WO2017155714A1 NOVEL CAS9 SYSTEMS AND METHODS OF USE 审中-公开
标题翻译：新型CAS9系统和使用方法
公开(公告)号：WO2017155714A1
公开(公告)日：2017-09-14
申请号：PCT/US2017/019624
申请日：2017-02-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： CIGAN, Andrew Mark , KING, Matthew G. , LIN, Haining , YOUNG, Joshua K.
IPC分类号： C12N15/113 , C12N9/22 , C12N15/82
摘要： Compositions and methods are provided for novel Cas9 systems, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The novel Cas9 systems described herein are derived from a CRISPR-Cas locus that differs from type II CRISPR-Cas loci, in that the novel CRISPR-Cas locus described herein contains multiple CRISPR arrays flanking a tracrRNA encoding region and cas9 gene and does not contain a cas1 gene and a cas2 gene. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
摘要翻译：为新的Cas9系统提供了组合物和方法，包括但不限于新的指导性多核苷酸/ Cas9内切核酸酶复合物，单或双引导RNA，引导RNA元件和Cas9内切核酸酶。本文描述的新型Cas9系统来源于不同于II型CRISPR-Cas基因座的CRISPR-Cas基因座，因为本文所述的新型CRISPR-Cas基因座包含侧接tracrRNA编码区和cas9基因且不含有多个CRISPR阵列 cas1基因和cas2基因。本公开还描述了用于基因组修饰细胞基因组中的靶序列，用于基因编辑以及用于将目的多核苷酸插入细胞基因组中的方法。

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