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1. 发明申请

WO2005054501A1 METHODS AND DEVICES FOR COMPOUND SCREENING 审中-公开
标题翻译：用于化合物筛选的方法和装置
公开(公告)号：WO2005054501A1
公开(公告)日：2005-06-16
申请号：PCT/EP2004/013379
申请日：2004-11-25
申请人： PAMGENE BV , VAN BEUNINGEN, Marinus, Gerardus, Johannes , BLOK, Herman, Jacobus
发明人： VAN BEUNINGEN, Marinus, Gerardus, Johannes , BLOK, Herman, Jacobus
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00378 , B01J2219/00387 , B01J2219/00427 , B01J2219/00641 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00734 , B01J2219/0074 , C40B40/06 , C40B40/10 , C40B60/14 , G01N2500/00
摘要： The present invention relates methods for screening of compounds for drug candidates comprising: (a) providing a solid porous support having first and second surfaces and at least one area with a plurality of through-going channels wherein said solid porous support comprises compounds within predefined regions of the said support; wherein said compounds within the porous structure are stored in dried condition, wherein said dried condition is obtained after a drying treatment by slow evaporation, vacuum drying or by blowing air or inert gas above and below said solid support; (b) providing a liquid sample comprising at least one molecular target; (c) mixing said dried compounds of step (a) with said liquid sample of step (b) by flow of the sample through said predefined regions of the solid support through the said through-going channels; (d) screening said compounds for drug candidates; said screening is by monitoring in an assay a compound-target interaction by measurement of a signal, said signal indicating interaction between a compound and a molecular target; (e) optionally screening for a compound having a putative effect on a drug candidate identified in step (d). The present invention further relates to the uses of said methods and solid supports and methods for the manufacture of said solid supports for carrying out said methods as well as to the use of a porous support for the preparation of a kit for carrying out said methods.
摘要翻译：本发明涉及用于筛选药物候选化合物的方法，包括：（a）提供具有第一和第二表面的固体多孔载体和至少一个具有多个通过通道的区域，其中所述固体多孔载体包含预定区域内的化合物的支持; 其中所述多孔结构体内的所述化合物在干燥状态下储存，其中所述干燥条件通过缓慢蒸发，真空干燥或通过吹入所述固体支持物上方和下方的空气或惰性气体进行干燥处理后获得; （b）提供包含至少一种分子靶的液体样品; （c）通过样品通过所述通过通道的固体支持物的所述预定区域的流动将步骤（a）的所述干燥的化合物与步骤（b）的所述液体样品混合; （d）筛选所述化合物用于候选药物; 所述筛选是通过在测定中监测化合物 - 靶相互作用，通过测量信号，所述信号指示化合物和分子靶之间的相互作用; （e）任选地筛选对步骤（d）中鉴定的药物候选物具有推定作用的化合物。本发明还涉及所述方法的用途和用于制备用于实施所述方法的所述固体支持物的固体支持物和方法，以及使用多孔载体来制备用于实施所述方法的试剂盒。

2. 发明申请

WO2003089137A1 SYSTEM, SUBSTRATE PLATE AND INCUBATION DEVICE FOR CONDUCTING BIOSSAYS 审中-公开
标题翻译：系统，用于导电BIOS的基板和插入装置
公开(公告)号：WO2003089137A1
公开(公告)日：2003-10-30
申请号：PCT/EP2003/050115
申请日：2003-04-17
申请人： PAMGENE B.V. , BLOK, Herman, Jacobus , VAN BEUNINGEN, Marinus, Gerardus, Johannes
发明人： BLOK, Herman, Jacobus , VAN BEUNINGEN, Marinus, Gerardus, Johannes
IPC分类号： B01L3/00
CPC分类号： B01L3/50255 , B01J2219/00315 , B01J2219/00317 , B01J2219/00495 , B01J2219/00641 , B01J2219/00662 , B01L3/50851 , B01L7/00 , B01L2200/0689 , B01L2200/12 , B01L2300/042 , B01L2300/0636 , B01L2300/0829 , B01L2400/0487 , B01L2400/049 , B29C66/472 , C40B60/14
摘要： A system for conducting bioassays comprises a substrate plate with wells, and an incubation device for holding the plate. The substrate plate comprises a microplate with an array of wells arranged in rows and columns, wherein the bottom of each well is a microarray substrate having oriented flow-through channels. The incubation device comprises an incubation chamber fox holding the microplate and a cover for sealing the incubation chamber. The incubation device has a heat block with array of openings, each opening adapted to receive a well of the microplate. A sealing gasket is provided for individually sealing each well of the microplate.
摘要翻译：用于进行生物测定的系统包括具有孔的基板和用于保持板的孵育装置。衬底板包括具有排列成行和列的孔阵列的微板，其中每个孔的底部是具有定向的流通通道的微阵列衬底。孵育装置包括保持微量培养板的孵育室狐和用于密封孵育室的盖。培养装置具有带有开口阵列的热块，每个开口适于接收微板的孔。提供密封垫片用于单独密封微板的每个孔。

3. 发明申请

WO2003089136A1 SUBSTRATE PLATE, METHOD AND APPARATUS FOR MANUFACTURING SUCH A SUBSTRATE PLATE, AND SYSTEM FOR CONDUCTING BIOSSAYS COMPRISUNG SUCH A SUBSTRATE PLATE 审中-公开
标题翻译：用于制造这种基板的基板，方法和装置，以及用于引导包含基板的BIOSS的系统
公开(公告)号：WO2003089136A1
公开(公告)日：2003-10-30
申请号：PCT/EP2003/050114
申请日：2003-04-17
申请人： PAMGENE B.V. , BLOK, Herman, Jacobus , VAN BEUNINGEN, Marinus, Gerardus, Johannes
发明人： BLOK, Herman, Jacobus , VAN BEUNINGEN, Marinus, Gerardus, Johannes
IPC分类号： B01L3/00
CPC分类号： B29C65/64 , B01J2219/00315 , B01J2219/00317 , B01J2219/00495 , B01J2219/00641 , B01J2219/00662 , B01L3/50255 , B01L3/50851 , B01L7/00 , B01L2200/0689 , B01L2200/12 , B01L2300/042 , B01L2300/0636 , B01L2300/0829 , B01L2400/0487 , B01L2400/049 , B29C65/02 , B29C65/18 , B29C65/242 , B29C65/245 , B29C65/44 , B29C65/48 , B29C65/7847 , B29C66/003 , B29C66/1122 , B29C66/21 , B29C66/24221 , B29C66/30326 , B29C66/472 , B29C66/542 , B29C66/63 , B29C66/71 , B29C66/73365 , B29C66/742 , B29C66/7461 , B29C66/81433 , B29C66/8181 , B29C66/8282 , B29C66/8322 , B29C66/8432 , B29C66/92445 , B29C66/9292 , B29C66/92921 , B29K2023/00 , B29K2709/02 , B29L2031/756 , C40B60/14 , B29K2023/38
摘要： A substrate plate comprises a microplate (1) made of a plastic material, having an array of wells (2) arranged in rows and columns, the bottom of at least one well (2) being provided by a porous substrate (3). Each porous substrate (3) is incorporated into the well (2) by means of a thermal bond.
摘要翻译：衬底板包括由塑料材料制成的微板（1），具有以行和列排列的孔阵列（2），至少一个孔（2）的底部由多孔基底（3）提供。每个多孔基材（3）通过热粘合结合到孔（2）中。

4. 发明申请

WO2007025594A1 METHOD FOR DETECTION AND QUANTIFICATION OF TARGET NUCLEIC ACIDS IN A SAMPLE 审中-公开
标题翻译：在样品中检测和定量目标核酸的方法
公开(公告)号：WO2007025594A1
公开(公告)日：2007-03-08
申请号：PCT/EP2006/006542
申请日：2006-07-05
申请人： PAMGENE BV , WU, Ying , VAN BEUNINGEN, Marinus, Gerardus, Johannes
发明人： WU, Ying , VAN BEUNINGEN, Marinus, Gerardus, Johannes
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6851 , C12Q2537/149 , C12Q2531/137 , C12Q2531/113
摘要： The present invention relates to methods for multiplex detection and quantification of target nucleic acid sequences in a sample comprising the steps of: (i) providing a solid support having immobilized thereon an array of detector oligonucleotides, wherein said array of detector oligonucleotides is designed by random selection of non-eukaryotic genomic sequences followed by random selection of oligonucleotide sequences and subsequent conversion of these oligonucleotide sequences such that these are composed of only three types of nucleotides; (ii) providing a sample having added thereto a fixed amount of control nucleic acid of known sequence; (iii) contacting said sample with at least two probes that hybridise to adjacent sites of a target sequence under conditions favouring hybridisation between the sample nucleic acids and the said at least two probes, wherein, a) a first probe is composed of a 5' end sequence part for hybridisation to a PCR primer and a 3' end sequence part for hybridisation to the target nucleic acid; and b) a second probe is composed of a 5' end sequence part for hybridisation to the target nucleic acid, and a 3' end sequence part for hybridisation with a PCR primer; and c) an intermediate sequence is present in between said 5' and 3' end sequence parts of said first or second probe; and d) said second probe is characterized by having a 5' phosphate group allowing ligation with a 3' hydroxyl group at the said first probe forming a ligation-mediated probe; (iv) ligation of the said hybridised first and second probes to form ligation-mediated probes; (v) contacting a set of detectable labelled PCR primers with the ligation-mediated probes allowing amplification thereof; (vi) detection and quantification of sample nucleic acids via hybridisation of the said intermediate parts within the amplified ligation-mediated probes onto the array of detector oligonucleotides provided in The present invention also relates to the use of said methods as well as microarrays and kits for performing said methods.
摘要翻译：本发明涉及在样品中多重检测和定量目标核酸序列的方法，其包括以下步骤：（i）提供在其上固定有检测器寡核苷酸阵列的固体支持物，其中所述检测器寡核苷酸阵列通过随机选择非真核生物基因组序列，然后随机选择寡核苷酸序列，随后转化这些寡核苷酸序列，使得它们仅由三种类型的核苷酸组成; （ii）提供具有已知序列的固定量的对照核酸的样品; （iii）在有利于样品核酸和所述至少两种探针之间的杂交的条件下，使所述样品与至少两种与靶序列的相邻位点杂交的探针接触，其中a）第一探针由5' 用于与PCR引物杂交的末端序列部分和用于与靶核酸杂交的3'末端序列部分; 和b）第二探针由用于与靶核酸杂交的5'末端序列部分和用于与PCR引物杂交的3'末端序列部分组成; 和c）所述第一或第二探针的所述5'和3'末端序列部分之间存在中间序列; 和d）所述第二探针的特征在于具有5'磷酸基，允许在所述第一探针处与3'羟基连接形成连接介导的探针; （iv）连接所述杂交的第一和第二探针以形成连接介导的探针; （v）将一组可检测的标记的PCR引物与连接介导的探针接触，允许其扩增; （vi）通过将扩增的连接介导的探针内的所述中间体部分杂交到本发明提供的检测器寡核苷酸阵列上来检测和定量样品核酸还涉及所述方法以及微阵列和试剂盒的用途执行所述方法。

5. 发明申请

WO2006037527A1 MASKED SOLID POROUS SUPPORTS ALLOWING FAST AND EASY REAGENT EXCHANGE TO ACCELERATE ELECTRODE-BASED MICROARRAYS 审中-公开
标题翻译：屏蔽固体多孔支持体，可快速，轻松地进行交换，以加速电极微型化
公开(公告)号：WO2006037527A1
公开(公告)日：2006-04-13
申请号：PCT/EP2005/010465
申请日：2005-09-28
申请人： PAMGENE BV , VAN BEUNINGEN, Marinus, Gerardus, Johannes , VAN DAMME, Hendrik, Sibolt , DE WIJN, Richard , HANNINK, Jurry, Maurice , INGHAM, Colin, John
发明人： VAN BEUNINGEN, Marinus, Gerardus, Johannes , VAN DAMME, Hendrik, Sibolt , DE WIJN, Richard , HANNINK, Jurry, Maurice , INGHAM, Colin, John
IPC分类号： G01N33/543
CPC分类号： G01N33/5438 , B01L3/502707 , B01L2300/0645 , B01L2300/12 , B01L2400/0415 , B01L2400/086 , G01N2035/00158
摘要： Solid porous supports find use in array analysis, as they offer high surface area for contacting the analyzed sample. The present invention provides a solid porous support suitable for array analysis having first and second surfaces, and comprising channels extending from said first surface to said second surface, characterised in that at least one conductive material is applied to predefined regions on said first surface and/or on said second surface and/or inside the channels contained within said solid porous support. Such conductive material(s) may form a high-precision grid delineating physically distinct compartments within the support and thus reduce the risk of cross-contamination in array analysis. Additionally, such conductive material(s) may directly participate in reactions performed on the array by means of their electrical and/or thermal conductivity.
摘要翻译：固体多孔载体用于阵列分析，因为它们提供高表面积以接触分析的样品。本发明提供了一种适于阵列分析的固体多孔载体，其具有第一和第二表面，并且包括从所述第一表面延伸到所述第二表面的通道，其特征在于，至少一种导电材料被施加到所述第一表面上的预定区域和/ 或在所述第二表面和/或包含在所述固体多孔载体内的通道的内部。这种导电材料可以形成描述支撑体内物理上不同的隔室的高精度网格，从而降低阵列分析中交叉污染的风险。另外，这种导电材料可以通过它们的电和/或导热性直接参与在阵列上进行的反应。

6. 发明申请

WO2003054551A1 NORMALISATION OF MICROARRAY DATA BASED ON HYBRIDISATION WITH AN INTERNAL REFERENCE 审中-公开
标题翻译：基于内部参考的混合微波数据的正规化
公开(公告)号：WO2003054551A1
公开(公告)日：2003-07-03
申请号：PCT/EP2002/014426
申请日：2002-12-17
申请人： PAMGENE B.V. , VAN BEUNINGEN, Marinus, Gerardus, Johannes
发明人： VAN BEUNINGEN, Marinus, Gerardus, Johannes
IPC分类号： G01N33/96
CPC分类号： C12Q1/68 , C12M1/34 , G01N33/48 , G01N33/50 , G01N35/00594 , G01N35/00693 , G01N2035/00158 , G01N2496/00 , G06F19/00
摘要： The invention relates to methods and corresponding arrays especially suited to correct for signal errors due to variations in sample preparation. Methods and compositions for performing quantitative array-based assays are provided. In the subject methods, both a reporter and an analyte is employed, where the reporter is characterized by binding selectively to an internal reference present on the array, i.e. at least a subset of, if not all of, the spots present on the array employed in the method contain an internal reference which can be bound by the reporter.
摘要翻译：本发明涉及特别适合于校正由于样品制备变化引起的信号误差的方法和相应阵列。提供了用于进行定量阵列测定的方法和组合物。在主题方法中，采用报告物和分析物，其中报道子的特征在于选择性地结合阵列上存在的内部参考，即至少一个子集，如果不是全部，则使用阵列上存在的斑点在该方法中包含可由报告人约束的内部参考。

7. 发明申请

WO2003047755A1 SYSTEM AND METHOD FOR ANALYZING A BLOOD SAMPLE AND DISPOSABLE CARTRIDGE FOR USE IN THIS SYSTEM OR METHOD 审中-公开
标题翻译：用于分析本系统或方法中使用的血液样品和可弃置的盒子的系统和方法
公开(公告)号：WO2003047755A1
公开(公告)日：2003-06-12
申请号：PCT/EP2002/013734
申请日：2002-12-03
申请人： PAMGENE B.V. , VAN BEUNINGEN, Marinus, Gerardus, Johannes
发明人： VAN BEUNINGEN, Marinus, Gerardus, Johannes
IPC分类号： B01L3/00
CPC分类号： B01L7/00 , B01L3/5027 , B01L3/502715 , B01L7/52 , B01L2200/0605 , B01L2300/0654 , B01L2300/0825 , B01L2400/0406 , B01L2400/065
摘要： A system for analyzing a blood sample comprises an element for nucleic acid (NA) isolation, an element for NA amplification, an incubator and a detector for detecting a parameter of the blood sample. The system comprises a single disposable cartridge having the NA isolation element and the NA amplification element, and a device having the incubator and the detector. The device is provided with a receiving space and each cartridge is adapted to be inserted into the receiving space. The NA amplification part of the cartridge is located within the incubator when the cartridge has been inserted into the receiving space.
摘要翻译：用于分析血液样品的系统包括用于核酸（NA）分离的元件，用于NA扩增的元件，用于检测血液样品参数的培养箱和检测器。该系统包括具有NA隔离元件和NA放大元件的单个一次性盒，以及具有培养箱和检测器的装置。设备设置有接收空间，并且每个盒适于插入接收空间。当墨盒已经插入接收空间时，墨盒的NA放大部分位于培养箱内。

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