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1. 发明申请

WO2004076557A1 BIODEGRADABLE POLYACETALS 审中-公开
标题翻译：生物可降解聚酯
公开(公告)号：WO2004076557A1
公开(公告)日：2004-09-10
申请号：PCT/US2004/005363
申请日：2004-02-24
申请人： NITTO DENKO CORPORATION , YU, Lei , VAN, Sang , JI, Shouping , MATSUMOTO, Kenji
发明人： YU, Lei , VAN, Sang , JI, Shouping , MATSUMOTO, Kenji
IPC分类号： C08L59/00
CPC分类号： C08G73/0213 , A61K47/59 , C08G65/002
摘要： A polymer comprising recurring units represented by formula (I); wherein X is selected from the group consisting of C(O)OR 1 , C(O)SR 1 , C(O)NR 1 R 2 , and VZ, where R 1 and R 2 are each individually selected from the group consisting of hydrogen, C 1 to C 10 alkyl, and C 6 to C 10 aryl, where V is a labile linker group, and where Z is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, and hexadecaamine dendrimer; and wherein Y is selected from the group consisting of -(CH 2 ) 2 -, -(CH 2 ) 2 -O-(CH 2 ) 2 -, -(CH 2 ) 2 -O-(CH 2 ) 2 -O-(CH 2 ) 2 -, and -(CH 2 ) 3 -NHC(O)-(CH 2 ) 6 -C(O)NH-(CH 2 ) 3 - is useful in nucleic acid delivery applications. Polyacetals of the formula (I) are preferably made by reacting appropriate diols and divinyl ethers. In preferred embodiments, complexes formed between polyacetals of the formula (I) and polynucleotides are useful as transfection reagents.
摘要翻译：一种包含由式（I）表示的重复单元的聚合物; 其中X选自C（O）OR 1，C（O）SR 1，C（O）NR 1 R 2和V Z，其中R 1和R 各自独立地选自氢，C1至C10烷基和C6至C10芳基，其中V是不稳定的连接基团，其中Z选自聚（乙烯亚胺），聚（乙烯亚胺）丙烯亚胺），聚（赖氨酸），PAMAM树枝状聚合物，八胺树枝状聚合物和十六胺树枝状聚合物; 并且其中Y选自 - （CH 2）2 - ， - （CH 2）2 -O-（CH 2）2 - ， - （CH 2）2 -O - （CH 2）2 -O - （CH 2）2 - 和 - （CH 2）3 -NHC（O） - （CH 2）6 -C（O）NH-（CH 2）3 - 可用于核酸递送应用。式（I）的聚缩醛优选通过使适当的二醇和二乙烯基醚反应来制备。在优选的实施方案中，在式（I）的聚缩醛和多核苷酸之间形成的络合物可用作转染试剂。

2. 发明申请

WO2005085458A3 POLYACETAL-PEPTIDE MEDIATED TRANSFECTION 审中-公开
公开(公告)号：WO2005085458A3
公开(公告)日：2005-09-15
申请号：PCT/US2005/005858
申请日：2005-02-24
申请人： NITTO DENKO CORPORATION , VAN, Sang , VIROONCHATAPAN, Nitnara , JI, Shouping , MATSUMOTO, Kenji , YU, Lei
发明人： VAN, Sang , VIROONCHATAPAN, Nitnara , JI, Shouping , MATSUMOTO, Kenji , YU, Lei
IPC分类号： C12N15/87 , A61K47/48
摘要： Complexes are disclosed which are a combination of a polynucleotide and a biodegradable polyacetal-peptide. The disclosed complexes are useful for cell transfection.

3. 发明申请

WO2003097107A3 CONTROLLABLY DEGRADABLE POLYMERIC BIOMOLECULE OR DRUG CARRIER AND METHOD OF SYNTHESIZING SAID CARRIER 审中-公开
公开(公告)号：WO2003097107A3
公开(公告)日：2003-11-27
申请号：PCT/US2003/015003
申请日：2003-05-13
申请人： NITTO DENKO CORPORATION , YU, Lei , DU, Fusheng , JI, Shouping , MATSUMOTO, Kenji
发明人： YU, Lei , DU, Fusheng , JI, Shouping , MATSUMOTO, Kenji
IPC分类号： A61K48/00
摘要： The present invention provides a controllably degradable cationic polymer for delivery of biomolecules (nucleic acids, peptides, etc.), drugs, molecules used in medical imaging applications, sensitizing agents used in cancer treatments, and molecules used in tissue engineering. The present invention also provides a method for synthesizing the polymer according to the present invention.

4. 发明申请

WO2003097107A2 CONTROLLABLY DEGRADABLE POLYMERIC BIOMOLECULE OR DRUG CARRIER AND METHOD OF SYNTHESIZING SAID CARRIER 审中-公开
标题翻译：控制性可降解的聚合物生物分子或药物载体和合成载体的方法
公开(公告)号：WO2003097107A2
公开(公告)日：2003-11-27
申请号：PCT/US2003/015003
申请日：2003-05-13
申请人： NITTO DENKO CORPORATION , YU, Lei , DU, Fusheng , JI, Shouping , MATSUMOTO, Kenji
发明人： YU, Lei , DU, Fusheng , JI, Shouping , MATSUMOTO, Kenji
IPC分类号： A61K48/00
CPC分类号： C08G73/02 , A61K47/59 , A61K47/593 , A61K47/60 , A61K47/645 , A61K48/0041
摘要： The present invention provides a controllably degradable cationic polymer for delivery of biomolecules (nucleic acids, peptides, etc.), drugs, molecules used in medical imaging applications, sensitizing agents used in cancer treatments, and molecules used in tissue engineering. The present invention also provides a method for synthesizing the polymer according to the present invention.
摘要翻译：本发明提供用于递送生物分子（核酸，肽等）的可控降解阳离子聚合物，用于医学成像应用的药物，分子，用于癌症治疗的敏化剂和组织工程中使用的分子。本发明还提供了合成本发明聚合物的方法。

5. 发明申请

WO2004065636A1 SOLID SURFACE FOR BIOMOLECULE DELIVERY AND HIGH-THROUGHPUT ASSAY 审中-公开
标题翻译：生物分子交付和高通量测定的固体表面
公开(公告)号：WO2004065636A1
公开(公告)日：2004-08-05
申请号：PCT/US2003/038498
申请日：2003-12-04
申请人： NITTO DENKO CORPORATION , YU, Lei , MATSUMOTO, Kenji , JI, Shouping , DU, Fusheng
发明人： YU, Lei , MATSUMOTO, Kenji , JI, Shouping , DU, Fusheng
IPC分类号： C12Q1/68
CPC分类号： C12N15/87 , A61K47/644 , A61K47/645 , A61K48/00
摘要： The present invention is related to a method for introducing biomolecules, such as nucleic acids, into cells by culturing cells on a solid surface which is coated with a transfection reagents and biomolecules.
摘要翻译：本发明涉及通过在被转染试剂和生物分子包被的固体表面上培养细胞而将生物分子如核酸引入细胞的方法。

6. 发明申请

WO2005023899A1 ACID-SENSITIVE POLYACETALS AND METHODS 审中-公开
标题翻译：酸敏感聚合物和方法
公开(公告)号：WO2005023899A1
公开(公告)日：2005-03-17
申请号：PCT/US2004/024284
申请日：2004-07-28
申请人： NITTO DENKO CORPORATION , VAN, Sang , VIROONCHATAPAN, Nitnara , JI, Shouping , YU, Lei
发明人： VAN, Sang , VIROONCHATAPAN, Nitnara , JI, Shouping , YU, Lei
IPC分类号： C08G4/00
CPC分类号： C08G4/00 , C07C229/22 , C08G73/028
摘要： Polyacetals comprising a recurring units represented by a formula selected from the group consisting of formula (I) and (II): wherein A comprises at least one acetal group; B is selected from the group consisting of -CH-, -CH(CH3)-, CH2CH-, -CH2C(CH3)-, -CH(CH3)CH-, and -CHCH(CH3)CH(CH3)-; Z is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VU; V is a linker group; U is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, hexadecaamine dendrimer, enhancer, and targeting receptor; R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl; D is a linkage selected from the group consisting of carboxylic amide, carboxylic ester, urea, and urethane; and G is selected from the group consisting of C4 to C20 alkyl, C6-C10 aryl, and -(OCH2CH2)n-, where n is in the range of 1 to about 250, are useful in nucleic acid delivery applications. In preferred embodiments, complexes formed between polyacetals comprising recurring units of the formulae (I) or (II) and polynucleotides are useful as transfection reagents.
摘要翻译：包含由选自式（I）和（II）的式所表示的重复单元的聚缩醛：其中A包含至少一个缩醛基; B选自-CH-，-CH（CH 3） - ，CH 2 CH - ， - CH 2 C（CH 3） - ， - CH（CH 3）CH-和-CHCH（CH 3）CH（CH 3） Z选自C（O）OR1，C（O）SR1，C（O）NR1R2和VU; V是连接基团; U选自聚（亚乙基亚胺），聚（丙烯亚胺），聚（赖氨酸），PAMAM树枝状聚合物，八胺树枝状聚合物，十六胺树枝状聚合物，增强剂和靶向受体; R 1和R 2各自独立地选自氢，C 1至C 10烷基和C 6至C 10芳基; D是选自羧酸酰胺，羧酸酯，脲和氨基甲酸酯的连接体; 并且G选自C 4至C 20烷基，C 6 -C 10芳基和 - （OCH 2 CH 2）n - ，其中n在1至约250的范围内，可用于核酸递送应用。在优选的实施方案中，在包含式（I）或（II）的重复单元的聚缩醛和多核苷酸之间形成的络合物可用作转染试剂。

7. 发明申请

WO2005083091A1 CULTURE DEVICE AND METHOD FOR EUKARYOTIC CELL TRANSFECTION 审中-公开
标题翻译：培养装置和用于真核细胞转移的方法
公开(公告)号：WO2005083091A1
公开(公告)日：2005-09-09
申请号：PCT/US2004/004818
申请日：2004-02-18
申请人： NITTO DENKO CORPORATION , TANAKA, Yasunobu , YU, Lei , JI, Shouping
发明人： TANAKA, Yasunobu , YU, Lei , JI, Shouping
IPC分类号： C12N15/64
CPC分类号： C12M23/12 , C12M35/00 , C12N15/87
摘要： A cell culture device for transfecting a eukaryotic cell is disclosed. The cell culture/transfection device may be a multiwell plate or slide which has been coated with a metal salt such as CaCl2. Methods of using the cell culture device to transfect mammalian cells and /or to monitor cell transfection are described. Kits are also described which include the cell transfection device, eukaryotic cells to be transformed and nucleic acid for transformation.
摘要翻译：公开了用于转染真核细胞的细胞培养装置。细胞培养/转染装置可以是已经用金属盐如CaCl 2涂覆的多孔板或载玻片。描述了使用细胞培养装置转染哺乳动物细胞和/或监测细胞转染的方法。还描述了包括细胞转染装置，待转化的真核细胞和用于转化的核酸的试剂盒。

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