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    • 2. 发明申请
    • 刃先付マイクロピペット保持装置および卵子細胞質内精子注入方法
    • 刀片式安装微型刀片保持装置及将其注射到卵巢细胞中的方法
    • WO2015174275A1
    • 2015-11-19
    • PCT/JP2015/062816
    • 2015-04-28
    • 株式会社北里サイエンス
    • 神野 正雄井上 太
    • C12M1/00C12N5/075C12N5/076G02B21/32
    • C12M23/48A61B17/43C12M21/06C12M35/00G02B21/32
    • 刃先付マイクロピペット保持装置1は、生体細胞に刺入するための刃先付マイクロピペット15を先端部に装着可能な筒状マイクロピペットホルダー5と、マイクロピペットホルダー5を保持するホルダー保持具2とを備える。ホルダー保持具2は、ベース部材20と、ベース部材20に回動可能に保持され、かつ、筒状マイクロピペットホルダー5が挿通可能な筒状回動部材3とを備え、さらに、筒状回動部材3は、筒状回動部材3の側面かつ筒状回動部材3の周方向に異なる位置に設けられた複数の側孔35と、側孔35より、筒状回動部材3内に侵入可能かつ、挿通するマイクロピペットホルダー5の外面に当接可能なホルダー位置調整部材62,72とからなるホルダー位置調整機構6,7を備えている。
    • 一种刀片式微量吸移管保持装置(1),其具有圆筒形微量吸管支架(5),所述微量吸管支架(5)允许用于穿刺活细胞的刀片安装的微量移液管(15)安装在其尖端, 以及保持所述微量移液管支架(5)的支架夹持工具(2)。 保持器保持工具(2)设置有基部构件(20)和圆柱形可旋转构件(3),所述圆柱形可旋转构件(3)以可旋转的方式被基座构件(20)保持,并且允许 圆筒形微量吸管支架(5)穿过其中。 此外,圆筒形可旋转构件(3)设置有包括多个侧孔(35)的保持器位置调节机构(6,7),所述侧孔(35)形成在圆筒形可旋转构件的侧面 3)在圆筒形可旋转构件(3)的圆周方向的不同位置处,以及保持器位置调整构件(62,72),所述保持器位置调节构件(62,72)能够进入圆筒形可旋转构件 )通过侧孔(35)并且能够抵接在插入的微量移液管保持器(5)的外表面上。
    • 4. 发明申请
    • MICROINJECTOR MOUNTING MODULE AND NEEDLE-HOLDER COMPONENT
    • 微型安装模块和针座组件
    • WO2013071898A3
    • 2013-07-11
    • PCT/CN2013070289
    • 2013-01-10
    • HUNAN CHANGSHA AO FIGURE BIOLOG TECHNOLOGY CO LTDPENG JIANJUN
    • PENG JIANJUN
    • C12M1/00
    • C12N15/89C12M33/04C12M35/00
    • A microinjector needle mounting module and a needle holder assembly, comprising a micro-injection needle (1), a needle seat (2) and a needle holder (3); the microinjector mounting module is formed by means of inserting the rear end of the microinjector (1) into a hole in the base (21) of the needle seat; the needle holder (3) is provided with an inner hole (32) passing through the barrel body; a conical socket (24) is provided inside the needle seat (2) and one end of the needle-holder (3) is provided with a conical connector (31), said conical connector (31) and conical socket (24) thus constituting a mutually-complementary insertion connection. In addition to a micro-injection needle being assembled with the needle seat to form a mounting module, other micro-operation needles such as holding pipettes or biopsy needles can also be assembled into a mounting module with said needle seat.
    • 一种微型注射针安装模块和针保持器组件,包括微注射针(1),针座(2)和针保持器(3); 微型注射器安装模块通过将微型注射器(1)的后端插入针座的基座(21)中的孔中而形成; 针保持器(3)设置有穿过筒体的内孔(32); 在针座(2)的内部设有锥形插座(24),针架(3)的一端设置有锥形连接器(31),所述锥形连接器(31)和锥形插座(24)构成 互补的插入连接。 除了与针座组装以形成安装模块的微型注射针之外,还可以将其它微操作针(例如保持移液管或活检针)组装到具有所述针座的安装模块中。
    • 7. 发明申请
    • ENHANCED LIPID PRODUCTION FROM ALGAE
    • 从ALGAE增强脂肪生产
    • WO2011035042A3
    • 2011-08-25
    • PCT/US2010049145
    • 2010-09-16
    • ROSEN BARRY H
    • ROSEN BARRY H
    • C12P7/64C12M1/36C12M3/02C12N1/12C12R1/89
    • C12P7/6463C12M21/02C12M35/00C12M41/06C12M41/12C12M41/32C12M43/02C12M47/10C12N1/12
    • A method for stimulating enhanced lipid accumulation by algae includes growing algae in a bioreactor medium including nutrients. The algae have an average lipid content that averages a first % of total cell biomass. A stress inducing environmental condition is initiated that keeps the algae alive, stops cell reproduction, and induces the algae to accumulate additional lipids resulting in a second average lipid content that is at least 50% more than the first %. The method can include measuring a lipid concentration of the algae while under the stress inducing environmental condition, harvesting lipids from more than 50% but not all of the algae when the lipid concentration is above a predetermined lipid limit, adding fresh medium to the bioreactor medium having algae not involved in the harvesting therein, and repeating the method, wherein the algae not involved in harvesting serves as a source for new algae growth.
    • 用于刺激藻类增强的脂质积累的方法包括在包括营养物质的生物反应器培养基中生长藻类。 藻类的平均脂质含量平均为总细胞生物量的第一%。 引发应激诱导环境条件,其保持藻类活跃,停止细胞繁殖,并诱导藻类积累额外的脂质,导致第二平均脂质含量比第一%高至少50%。 该方法可以包括在应激诱导环境条件下测量藻类的脂质浓度,当脂质浓度高于预定脂质限度时,从超过50%而不是全部藻类中收获脂质,向生物反应器培养基中加入新鲜培养基 藻类不参与其中的收获,并且重复该方法,其中不涉及收获的藻类作为新藻类生长的来源。
    • 8. 发明申请
    • INTRACELLULAR MOLECULAR DELIVERY BASED ON NANOSTRUCTURE INJECTORS
    • 基于纳米结构引物的细胞分子分析
    • WO2008133755A3
    • 2009-01-08
    • PCT/US2007088323
    • 2007-12-20
    • UNIV CALIFORNIACHEN XINGBERTOZZI CAROLYNZETTL ALEXANDER
    • CHEN XINGBERTOZZI CAROLYNZETTL ALEXANDER
    • C12M1/00
    • C12M35/00
    • There is disclosed a method and device for the delivery of molecules into individual cells. A device for injecting a biological molecule into a target cell comprises a microscopic tip attached to a mechanical scanning device for positioning the tip relative to the target cell and for moving the tip into the target cell; a nanostructure, such as a carbon nanotube, fixed on an end of the microscopic tip; and a biological molecule attached to the nanotube by a cleavable electrostatic or chemical linker linking the biomolecule to the nanotube, said chemical linker having a chemical linkage which is cleaved in an intracellular environment. The biological molecule may be one or more of proteins, nucleic acids, small molecule drugs, and optical labels, and combinations thereof. Exemplified are multiple walled carbon nanotubes to which a polycyclic aromatic compound is adsorbed, the aromatic compound having a side chain containing a cleavable disulfide linkage and a biotin functionality for coupling to a streptavidin-linked payload.
    • 公开了用于将分子递送到单个细胞中的方法和装置。 用于将生物分子注入靶细胞的装置包括附接到机械扫描装置的微小尖端,用于相对于靶细胞定位尖端并将尖端移动到靶细胞中; 固定在微小尖端的端部的纳米结构,例如碳纳米管; 以及通过将生物分子连接到纳米管的可切割静电或化学连接子连接到纳米管的生物分子,所述化学连接体具有在细胞内环境中被切割的化学连接。 生物分子可以是蛋白质,核酸,小分子药物和光学标记物中的一种或多种,​​以及它们的组合。 例示了吸附有多环芳族化合物的多壁碳纳米管,具有含有可切割二硫键的侧链的芳族化合物和与链亲和素链接的有效负载偶联的生物素功能。
    • 10. 发明申请
    • ROTATIONALLY OSCILLATING INJECTOR
    • 旋转式振荡式注射器
    • WO2008046051A2
    • 2008-04-17
    • PCT/US2007081266
    • 2007-10-12
    • UNIV CONNECTICUTOLGAC NEJAT
    • OLGAC NEJAT
    • A61M5/46
    • C12M35/00C12M21/06
    • A microinjection device is provided that includes an injection element defining a longitudinal axis, and that further includes a motor. The injection element is rotatable about the longitudinal axis by the rotational motor. The injection element is for penetrating a target, such as a cell. A microinjection system is provided that includes the microinjection device and a control unit. The control unit is for controlling a rotational amplitude and a frequency of oscillation of the injection element. A method for penetrating a target to facilitate injecting material therein is provided that includes providing the material to an injection element, contacting the target with a distal end of the injection element, rotating the injection element about a longitudinal axis to form a hole in the target, and penetrating the target with the injection element via the hole formed in the target. A method for performing intra-cytoplasmic sperm injection is provided that includes providing a solution comprising sperm to an injection element, contacting an oocyte with a distal end of the injection element, rotating the injection element alternately clockwise and counterclockwise about a longitudinal axis to form a hole in the oocyte, penetrating the oocyte with the distal end of the injection element via the hole formed in the oocyte, and expelling the solution comprising sperm into the penetrated oocyte.
    • 提供了一种显微注射装置,其包括限定纵向轴线的注射元件,并且还包括马达。 注射元件可以通过旋转电动机围绕纵向轴线旋转。 注射元件用于穿透靶,例如细胞。 提供了一种显微注射系统,其包括显微注射装置和控制单元。 控制单元用于控制喷射元件的旋转振幅和振荡频率。 提供了一种用于穿透目标以便于在其中注入材料的方法,其包括将材料提供给注射元件,使靶与注射元件的远端接触,使注射元件围绕纵向轴线旋转以在靶中形成孔 并且通过形成在靶中的孔用注射元件穿透靶。 提供了一种用于进行细胞质内精子注射的方法,其包括向注射元件提供包含精子的溶液,使卵母细胞与注射元件的远端接触,使注射元件围绕纵轴交替地顺时针和逆时针旋转,以形成 在卵母细胞中的孔,通过在卵母细胞中形成的孔穿过注射元件的远端的卵母细胞,并将包含精子的溶液排出到穿透的卵母细胞中。