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    • 5. 发明申请
    • METHODOLOGY FOR DETECTION, ENUMERATION, PROPAGATION AND MANIPULATION OF BACTERIOPHAGES
    • 检测,产生,传播和控制杀虫剂的方法
    • WO2008094202A2
    • 2008-08-07
    • PCT/US2007/017000
    • 2007-07-27
    • ROTH, JonathanLARKIN, JamesHSU, Fu-ChihWONG, Choi-lok, RebeccaBATTIGELLI, David, A.ROTH, Geoffrey, N.
    • ROTH, JonathanLARKIN, JamesHSU, Fu-ChihWONG, Choi-lok, RebeccaBATTIGELLI, David, A.ROTH, Geoffrey, N.
    • C12Q1/04C12Q1/045
    • A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature- independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages.
    • 设计了一种在水样品或其他含水样品中繁殖,枚举和定量噬菌体的方法,其包含刺激特定噬菌体感染的选择细菌物种生长的成分,其中干扰背景生物 被抑制或无关紧要。 培养基的重要特征包括产生有色和/或荧光产物的氧化还原化合物,显色和/或荧光酶底物和与温度无关的胶凝剂。 优选的组合是含有2,3,5-三苯基四唑氯化物,5-溴-4-氯-3-吲哚基-β-半乳糖苷和适当的胶凝剂的生长培养基(当适当使用时)产生深红色细菌 草坪含有蓝绿色,不规则圆形的斑点,代表单个噬菌体斑块,在可见光下都可以看出眼睛。 该程序也可以方便地应用于人造照明下的自动计数系统。 该程序可以与水样和洗脱缓冲液一起使用,洗脱缓冲液可以在缓冲液与食物,土壤,硬表面和可能被噬菌体污染的其它固体接触后保持悬浮状态。
    • 6. 发明申请
    • BUILDING CONSTRUCTION ASSEMBLY OF STRUCTURAL MODULES
    • 建筑结构模块的建筑装配
    • WO2003072884A1
    • 2003-09-04
    • PCT/IL2003/000149
    • 2003-02-26
    • DYNAMIC SHELLS LTD.ROTH, Jonathan
    • ROTH, Jonathan
    • E04B1/12
    • E04B1/12E04B1/6158E04B1/6175E04C2/22E04C2/34
    • A building construction assembly composed of factory-manufactured thermoplastic structural modules which when interconnected at a building site then create the entire shell of an edifice having side walls and a roof. Each module which is formed of a pair of parallel panels bridged by transverse webs is provided on either side along its length with a pair of flexible catch flanges projecting from the panels. When assembling these modules to create the shell of the edifice, adjacent modules are interconnected in coplanar relation by a double-faced joiner on each face of which is a pair of teeth. When one face of the joiner is pressed against a side of a module, its catch flanges then flex to snap into and behind the teeth to connect the joiner to this module. The other face of the joiner is similarly connected to the side of an adjacent module whereby the modules are then interconnected in coplanar relation. When it becomes necessary to interconnect four modules in a cross formation, use is then made of a square, quad-faced joiner on each face of which are a pair of teeth which are engageable by the catch flanges projecting from the side of the module to be joined to this face.
    • 由工厂制造的热塑性结构模块组成的建筑施工组件,当在建筑工地相互连接时,建造具有侧壁和屋顶的大厦的整个外壳。 由横向腹板桥接的一对平行面板形成的每个模块在其长度的两侧设置有一对从面板突出的柔性捕获凸缘。 当组装这些模块以形成大厦的外壳时,相邻的模块通过双面连接器在共面关系中互相连接,双面接头在其每个面上都是一对齿。 当连接器的一个面压靠在模块的一侧时,其卡扣凸缘然后弯曲以卡入到齿内和后面,以将连接器连接到该模块。 接合器的另一面类似地连接到相邻模块的侧面,由此模块以共面关系互连。 当需要在交叉形式中互连四个模块时,然后使用正方形的四面连接器,其中每个面都是一对齿,其可以通过从模块的侧面突出的捕获凸缘接合到 加入这个脸。