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1. 发明申请

WO2008094202A2 METHODOLOGY FOR DETECTION, ENUMERATION, PROPAGATION AND MANIPULATION OF BACTERIOPHAGES 审中-公开
标题翻译：检测，产生，传播和控制杀虫剂的方法
公开(公告)号：WO2008094202A2
公开(公告)日：2008-08-07
申请号：PCT/US2007/017000
申请日：2007-07-27
申请人： ROTH, Jonathan , LARKIN, James , HSU, Fu-Chih , WONG, Choi-lok, Rebecca , BATTIGELLI, David, A. , ROTH, Geoffrey, N.
发明人： ROTH, Jonathan , LARKIN, James , HSU, Fu-Chih , WONG, Choi-lok, Rebecca , BATTIGELLI, David, A. , ROTH, Geoffrey, N.
CPC分类号： C12Q1/04 , C12Q1/045
摘要： A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature- independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages.
摘要翻译：设计了一种在水样品或其他含水样品中繁殖，枚举和定量噬菌体的方法，其包含刺激特定噬菌体感染的选择细菌物种生长的成分，其中干扰背景生物被抑制或无关紧要。培养基的重要特征包括产生有色和/或荧光产物的氧化还原化合物，显色和/或荧光酶底物和与温度无关的胶凝剂。优选的组合是含有2,3,5-三苯基四唑氯化物，5-溴-4-氯-3-吲哚基-β-半乳糖苷和适当的胶凝剂的生长培养基（当适当使用时）产生深红色细菌草坪含有蓝绿色，不规则圆形的斑点，代表单个噬菌体斑块，在可见光下都可以看出眼睛。该程序也可以方便地应用于人造照明下的自动计数系统。该程序可以与水样和洗脱缓冲液一起使用，洗脱缓冲液可以在缓冲液与食物，土壤，硬表面和可能被噬菌体污染的其它固体接触后保持悬浮状态。

2. 发明申请

WO2008094202A3 METHODOLOGY FOR DETECTION, ENUMERATION, PROPAGATION AND MANIPULATION OF BACTERIOPHAGES 审中-公开
标题翻译：用于检测，测定，传播和操作菌的方法学
公开(公告)号：WO2008094202A3
公开(公告)日：2008-10-09
申请号：PCT/US2007017000
申请日：2007-07-27
申请人： ROTH JONATHAN , LARKIN JAMES , HSU FU-CHIH , WONG CHOI-LOK REBECCA , BATTIGELLI DAVID A , ROTH GEOFFREY N
发明人： ROTH JONATHAN , LARKIN JAMES , HSU FU-CHIH , WONG CHOI-LOK REBECCA , BATTIGELLI DAVID A , ROTH GEOFFREY N
IPC分类号： C12Q1/70 , G01N33/569
CPC分类号： C12Q1/04 , C12Q1/045
摘要： A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature- independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages.
摘要翻译：设计了一种在水样或其他含水样品中增殖，计数和定量噬菌体的方法，该方法含有刺激容易被特定噬菌体感染的选定细菌物种的生长的成分，其中干扰背景生物受到抑制或无关紧要。培养基的重要特征包括产生有色和/或荧光产物的氧化还原化合物，发色和/或荧光酶底物和温度不依赖性胶凝剂。优选的组合是含有2,3,5-三苯基四唑鎓氯化物，5-溴-4-氯-3-吲哚基-D-半乳糖苷和合适的胶凝剂的生长培养基，其（当适当使用时）产生深红色细菌含有深蓝绿色的草坪，代表单个噬菌斑的不规则圆形斑点，在可见光下全部可见。该程序也可以很容易地应用于人工照明下的自动计数系统。该程序可用于水样品和洗脱缓冲液，缓冲液与食品，土壤，硬表面和其他可能被噬菌体污染的固体接触后可保留噬菌体悬浮液。

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