专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2011039519A3 ACETYL LYSINE INCORPORATION WITH tRNA SYNTHETASE 审中-公开
标题翻译：乙酰胆碱配合tRNA合成酶
公开(公告)号：WO2011039519A3
公开(公告)日：2011-06-23
申请号：PCT/GB2010001848
申请日：2010-10-01
申请人： MEDICAL RES COUNCIL UNITED KINGDOM , NEUMANN HEINZ , CHIN JASON
发明人： NEUMANN HEINZ , CHIN JASON
IPC分类号： C12N9/00 , C12P21/02
CPC分类号： C12P21/02 , C12N9/93
摘要： The invention relates to a tRNA synthetase capable of binding Ne-acetyl lysine, wherein said synthetase comprises a polypeptide having at least 90 % sequence identity to the amino acid sequence of MbPyIRS, and wherein said synthetase comprises a L266M mutation.
摘要翻译：本发明涉及能够结合新乙酰赖氨酸的tRNA合成酶，其中所述合成酶包含与MbPyIRS的氨基酸序列具有至少90％序列同一性的多肽，其中所述合成酶包含L266M突变。

2. 发明申请

WO2010139948A2 METHODS 审中-公开
标题翻译：方法
公开(公告)号：WO2010139948A2
公开(公告)日：2010-12-09
申请号：PCT/GB2010001083
申请日：2010-06-02
申请人： MEDICAL RES COUNCIL , UNIV NORTH CAROLINA STATE , NGUYEN DUY P , NEUMANN HEINZ , DEITERS ALEXANDER , CHIN JASON , LUSIC HRVOJE
发明人： NGUYEN DUY P , NEUMANN HEINZ , DEITERS ALEXANDER , CHIN JASON , LUSIC HRVOJE
IPC分类号： C12N15/70
CPC分类号： C12N15/67 , C07C233/49 , C07C271/22 , C12N9/93 , C12P21/02
摘要： The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.
摘要翻译：本发明涉及一种制备包含正交官能团的多肽的方法，所述正交官能团由脂肪族氨基酸或氨基酸衍生物构成，所述方法包括提供宿主细胞; 提供编码目的多肽的核酸; 提供与所述宿主细胞正交的tRNA-tRNA合成酶对; 添加包含所述正交官能团的氨基酸或氨基酸衍生物，其中所述氨基酸或氨基酸衍生物是所述正交tRNA合成酶的底物，其中所述氨基酸或氨基酸衍生物具有脂族碳骨架; 并孵育以允许所述氨基酸或氨基酸衍生物通过正交的tRNA-tRNA合成酶对引入目的多肽。本发明还涉及某些氨基酸，以及包含其的多肽。

3. 发明申请

WO2008065398A3 EVOLVED ORTHOGONAL RIBOSOMES 审中-公开
标题翻译：进化的正交RIBOSOMES
公开(公告)号：WO2008065398A3
公开(公告)日：2008-07-17
申请号：PCT/GB2007004562
申请日：2007-11-28
申请人： MEDICAL RES COUNCIL , CHIN JASON , WANG KAIHANG , NEUMANN HEINZ
发明人： CHIN JASON , WANG KAIHANG , NEUMANN HEINZ
IPC分类号： C12N15/11
CPC分类号： C12N15/11 , C12N15/111 , C12N2320/13 , C12P21/02 , C40B10/00 , C40B40/08
摘要： There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.
摘要翻译：提供了用于演化正交rRNA分子的方法，包括以下步骤：提供一个或多个突变正交rRNA分子文库，并将所述文库引入细胞中，使得正交rRNA并入核糖体以提供正交核糖体; 提供（i）不被天然核糖体翻译并且（ii）包含一个或多个正交mRNA密码子的一个或多个正交mRNA分子; 测定正交mRNA的翻译并选择翻译正交mRNA的正交rRNA分子，其中步骤（c）中的测定需要在正交mRNA中翻译一个或多个正交mRNA密码子; 和掺入这种rRNA分子的正交核糖体。

4. 发明申请

WO2011107747A3 GENETICALLY ENCODED PHOTOCONTROL 审中-公开
标题翻译：基因编码的光控制
公开(公告)号：WO2011107747A3
公开(公告)日：2011-12-29
申请号：PCT/GB2011000304
申请日：2011-03-04
申请人： MEDICAL RES COUNCIL , CHIN JASON , NGUYEN DUY P , GAUTIER ARNAUD , DIETERS ALEXANDER , UNIV NORTH CAROLINA
发明人： CHIN JASON , NGUYEN DUY P , GAUTIER ARNAUD , DIETERS ALEXANDER
IPC分类号： C12N9/00 , C12N9/10 , C12N15/74
CPC分类号： C12N9/1205 , C07D317/50 , C07K14/805 , C12N9/93 , C12Y207/12002 , C12Y601/01026
摘要： The invention relates to a caged lysine, wherein the caged lysine is according to Formula (I): or salts thereof. The invention further relates to polypeptides comprising a caged lysine, and to methods of making same. The invention further relates to tRNA synthetases capable of charging tRNA with caged lysine.
摘要翻译：本发明涉及笼蔽赖氨酸，其中笼蔽赖氨酸根据式（I）：或其盐。本发明还涉及包含笼蔽赖氨酸的多肽，并涉及制备它的方法。本发明进一步涉及能够用笼蔽赖氨酸对tRNA进行充电的tRNA合成酶。

5. 发明申请

WO2010028366A2 METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING VALIDATION, CALIBRATION AND NORMALIZATION 审中-公开
标题翻译：用于核酸测序，校准和正常化的方法和系统
公开(公告)号：WO2010028366A2
公开(公告)日：2010-03-11
申请号：PCT/US2009056225
申请日：2009-09-08
申请人： LIFE TECHNOLOGIES CORP , GREINER DOUGLAS P , GJERSTAD CARMEN , ZIEGLE JANET S , JONES LEE W , SHEN MIN-YI , CHIN JASON , BREU HEINZ
发明人： GREINER DOUGLAS P , GJERSTAD CARMEN , ZIEGLE JANET S , JONES LEE W , SHEN MIN-YI , CHIN JASON , BREU HEINZ
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6874 , C12Q2545/101 , C12Q2563/149
摘要： A system for performing quality control for nucleic acid sample sequencing is disclosed. The system has a set of solid supports, each support having attached thereto a plurality of nucleic acid sequences. The set has plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived. A method of preparing a quality control for performing nucleic acid sample sequencing and a method of validating a nucleic acid sequencing instrument are also disclosed.
摘要翻译：公开了一种用于执行核酸样品测序的质量控制的系统。该系统具有一组固体支持物，每个支持物附着有多个核酸序列。该组具有多组固体支持物，并且每组包含具有与其相连的相同核酸序列的固体支持物。各组的核酸序列彼此不同。核酸序列是合成来源的。还公开了制备用于执行核酸样品测序的质量控制的方法和验证核酸测序仪器的方法。

6. 发明申请

WO2011107747A8 GENETICALLY ENCODED PHOTOCONTROL 审中-公开
标题翻译：基因编码光子
公开(公告)号：WO2011107747A8
公开(公告)日：2012-09-27
申请号：PCT/GB2011000304
申请日：2011-03-04
申请人： MEDICAL RES COUNCIL , CHIN JASON , NGUYEN DUY P , GAUTIER ARNAUD , DEITERS ALEXANDER , UNIV NORTH CAROLINA STATE
发明人： CHIN JASON , NGUYEN DUY P , GAUTIER ARNAUD , DEITERS ALEXANDER
IPC分类号： C12N9/00 , C12N9/10 , C12N15/74
CPC分类号： C12N9/1205 , C07D317/50 , C07K14/805 , C12N9/93 , C12Y207/12002 , C12Y601/01026
摘要： The invention relates to a caged lysine, wherein the caged lysine is according to Formula (I): or salts thereof. The invention further relates to polypeptides comprising a caged lysine, and to methods of making same. The invention further relates to tRNA synthetases capable of charging tRNA with caged lysine.
摘要翻译：本发明涉及笼状赖氨酸，其中笼养的赖氨酸是根据式（I）：或其盐。本发明进一步涉及包含笼养赖氨酸的多肽及其制备方法。本发明还涉及能够将tRNA与笼养的赖氨酸一起加入的tRNA合成酶。

7. 发明申请

WO2012175924A3 INCORPORATION OF SUBSTITUTED LYSINES INTO POLYPEPTIDES 审中-公开
标题翻译：将替代的LYSINES纳入多肽中
公开(公告)号：WO2012175924A3
公开(公告)日：2013-05-23
申请号：PCT/GB2012000543
申请日：2012-06-22
申请人： MEDICAL RES COUNCIL , CHIN JASON , VIRDEE SATPAL
发明人： CHIN JASON , VIRDEE SATPAL
IPC分类号： C12N9/00 , C07K19/00 , C12N15/52
CPC分类号： C12P21/00 , C07C271/22 , C07C323/59 , C07K2319/95 , C12N9/104 , C12N9/93 , C12Y601/01026
摘要： The invention related to a tRNA synthetase capable of binding delta-substituted lysine, wherein said tRNA synthetase comprises amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS, wherein said sequence comprises 5 or fewer substitutions within the amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS; and wherein said synthetase comprises W at amino acid position 349 relative to MbPyIRS.
摘要翻译：本发明涉及能够结合δ-取代的赖氨酸的tRNA合成酶，其中所述tRNA合成酶包含对应于MbPyIRS的至少L271至Y349的氨基酸序列的氨基酸序列，其中所述序列在氨基酸内包含5个或更少个取代对应于MbPyIRS的至少L271至Y349的氨基酸序列的序列; 并且其中所述合成酶在相对于MbPyIRS的氨基酸位置349处包含W。

8. 发明申请

WO2011039518A2 INCORPORATION OF METHYL LYSINE INTO POLYPEPTIDE 审中-公开
标题翻译：将甲基赖氨酸加入多肽中
公开(公告)号：WO2011039518A2
公开(公告)日：2011-04-07
申请号：PCT/GB2010001847
申请日：2010-10-01
申请人： MEDICAL RES COUNCIL , CHIN JASON , NGUYEN DUY P
发明人： CHIN JASON , NGUYEN DUY P
IPC分类号： C12N9/00
CPC分类号： C07K14/47 , C12N9/93 , C12P21/02 , G01N33/566
摘要： The invention relates to A method of making a polypeptide comprising at least one Ne-methyl-lysine at a specific site in said polypeptide, said method comprising (a) genetically directing the incorporation of R-Ne-methyl-lysine into said polypeptide, wherein R comprises an auxiliary group; and (b) catalysing the removal of R from the polypeptide of (a). In particular the invention relates to such a method wherein genetically directing the incorporation of R- Ne-methyl-lysine into said polypeptide comprises arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, and wherein said translation is carried out in the presence of an amber tRNA charged with R-Ne-methyl-lysine.
摘要翻译：本发明涉及一种在所述多肽的特定位点制备包含至少一个Ne-甲基 - 赖氨酸的多肽的方法，所述方法包括（a）将R-Ne-甲基赖氨酸引入所述多肽中，其中 R包括辅助基团; 和（b）催化从（a）的多肽中除去R。特别地，本发明涉及这样一种方法，其中将R-Ne-甲基 - 赖氨酸掺入到所述多肽中的方法包括安排编码所述多肽的RNA的翻译，其中所述RNA包含琥珀密码子，并且其中所述翻译是在带有R-Ne-甲基 - 赖氨酸的琥珀色tRNA的存在下进行。

9. 发明申请

WO2011117583A3 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2011117583A3
公开(公告)日：2012-01-12
申请号：PCT/GB2011000420
申请日：2011-03-23
申请人： MEDICAL RES COUNCIL , CHIN JASON , NGUYEN DUY P , VIRDEE SATPAL
发明人： CHIN JASON , NGUYEN DUY P , VIRDEE SATPAL
IPC分类号： C07K14/00 , C12N9/00
CPC分类号： C07K14/4702 , C07K1/064 , C07K1/065 , C07K1/068 , C07K1/1075 , C07K1/1077 , C07K14/435 , C12N9/50 , C12N9/93 , C12P21/02 , C12P21/06 , Y02P20/55
摘要： The invention relates to a method of modifying a specific lysine residue in a polypeptide comprising at least two lysine residues, said method comprising (a) providing a polypeptide comprising a target lysine residue protected by a first protecting group, and at least one further lysine residue; (b) treating the polypeptide to protect said further lysine residue(s), wherein the protecting group for said further lysine residues is different to the protecting group for the target lysine residue; (c) selectively deprotecting the target lysine residue; and (d) modifying the deprotected lysine residue of (c).
摘要翻译：本发明涉及一种修饰包含至少两个赖氨酸残基的多肽中的特定赖氨酸残基的方法，所述方法包括（a）提供包含被第一保护基团保护的靶赖氨酸残基的多肽和至少一种赖氨酸残基 ; （b）处理多肽以保护所述另外的赖氨酸残基，其中所述另外的赖氨酸残基的保护基与靶赖氨酸残基的保护基不同; （c）选择性脱保护目标赖氨酸残基; 和（d）修饰（c）的去保护的赖氨酸残基。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式