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1. 发明申请

WO2011039518A3 INCORPORATION OF METHYL LYSINE INTO POLYPEPTIDES 审中-公开
公开(公告)号：WO2011039518A3
公开(公告)日：2011-04-07
申请号：PCT/GB2010/001847
申请日：2010-10-01
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , NGUYEN, Duy, P.
发明人： CHIN, Jason , NGUYEN, Duy, P.
IPC分类号： C12N9/00 , C12P21/02
摘要： The invention relates to A method of making a polypeptide comprising at least one N ε -methyl-lysine at a specific site in said polypeptide, said method comprising (a) genetically directing the incorporation of R-N ε -methyl-lysine into said polypeptide, wherein R comprises an auxiliary group; and (b) catalysing the removal of R from the polypeptide of (a). In particular the invention relates to such a method wherein genetically directing the incorporation of R- Nε-methyl-lysine into said polypeptide comprises arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, and wherein said translation is carried out in the presence of an amber tRNA charged with R-N ε -methyl-lysine.

2. 发明申请

WO2011039518A2 INCORPORATION OF METHYL LYSINE INTO POLYPEPTIDE 审中-公开
标题翻译：将甲基赖氨酸加入多肽中
公开(公告)号：WO2011039518A2
公开(公告)日：2011-04-07
申请号：PCT/GB2010001847
申请日：2010-10-01
申请人： MEDICAL RES COUNCIL , CHIN JASON , NGUYEN DUY P
发明人： CHIN JASON , NGUYEN DUY P
IPC分类号： C12N9/00
CPC分类号： C07K14/47 , C12N9/93 , C12P21/02 , G01N33/566
摘要： The invention relates to A method of making a polypeptide comprising at least one Ne-methyl-lysine at a specific site in said polypeptide, said method comprising (a) genetically directing the incorporation of R-Ne-methyl-lysine into said polypeptide, wherein R comprises an auxiliary group; and (b) catalysing the removal of R from the polypeptide of (a). In particular the invention relates to such a method wherein genetically directing the incorporation of R- Ne-methyl-lysine into said polypeptide comprises arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, and wherein said translation is carried out in the presence of an amber tRNA charged with R-Ne-methyl-lysine.
摘要翻译：本发明涉及一种在所述多肽的特定位点制备包含至少一个Ne-甲基 - 赖氨酸的多肽的方法，所述方法包括（a）将R-Ne-甲基赖氨酸引入所述多肽中，其中 R包括辅助基团; 和（b）催化从（a）的多肽中除去R。特别地，本发明涉及这样一种方法，其中将R-Ne-甲基 - 赖氨酸掺入到所述多肽中的方法包括安排编码所述多肽的RNA的翻译，其中所述RNA包含琥珀密码子，并且其中所述翻译是在带有R-Ne-甲基 - 赖氨酸的琥珀色tRNA的存在下进行。

3. 发明申请

WO2011117583A3 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2011117583A3
公开(公告)日：2012-01-12
申请号：PCT/GB2011000420
申请日：2011-03-23
申请人： MEDICAL RES COUNCIL , CHIN JASON , NGUYEN DUY P , VIRDEE SATPAL
发明人： CHIN JASON , NGUYEN DUY P , VIRDEE SATPAL
IPC分类号： C07K14/00 , C12N9/00
CPC分类号： C07K14/4702 , C07K1/064 , C07K1/065 , C07K1/068 , C07K1/1075 , C07K1/1077 , C07K14/435 , C12N9/50 , C12N9/93 , C12P21/02 , C12P21/06 , Y02P20/55
摘要： The invention relates to a method of modifying a specific lysine residue in a polypeptide comprising at least two lysine residues, said method comprising (a) providing a polypeptide comprising a target lysine residue protected by a first protecting group, and at least one further lysine residue; (b) treating the polypeptide to protect said further lysine residue(s), wherein the protecting group for said further lysine residues is different to the protecting group for the target lysine residue; (c) selectively deprotecting the target lysine residue; and (d) modifying the deprotected lysine residue of (c).
摘要翻译：本发明涉及一种修饰包含至少两个赖氨酸残基的多肽中的特定赖氨酸残基的方法，所述方法包括（a）提供包含被第一保护基团保护的靶赖氨酸残基的多肽和至少一种赖氨酸残基 ; （b）处理多肽以保护所述另外的赖氨酸残基，其中所述另外的赖氨酸残基的保护基与靶赖氨酸残基的保护基不同; （c）选择性脱保护目标赖氨酸残基; 和（d）修饰（c）的去保护的赖氨酸残基。

4. 发明申请

WO2011107747A2 GENETICALLY ENCODED PHOTOCONTROL 审中-公开
标题翻译：基因编码光子
公开(公告)号：WO2011107747A2
公开(公告)日：2011-09-09
申请号：PCT/GB2011/000304
申请日：2011-03-04
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , NGUYEN, Duy, P. , GAUTIER, Arnaud , DIETERS, Alexander , THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
发明人： CHIN, Jason , NGUYEN, Duy, P. , GAUTIER, Arnaud , DIETERS, Alexander
CPC分类号： C12N9/1205 , C07D317/50 , C07K14/805 , C12N9/93 , C12Y207/12002 , C12Y601/01026
摘要： The invention relates to a caged lysine, wherein the caged lysine is according to Formula (I): or salts thereof. The invention further relates to polypeptides comprising a caged lysine, and to methods of making same. The invention further relates to tRNA synthetases capable of charging tRNA with caged lysine.
摘要翻译：本发明涉及笼状赖氨酸，其中笼养的赖氨酸是根据式（I）：或其盐。本发明进一步涉及包含笼养赖氨酸的多肽及其制备方法。本发明还涉及能够将tRNA与笼养的赖氨酸一起加入的tRNA合成酶。

5. 发明申请

WO2010139948A2 METHODS 审中-公开
标题翻译：方法
公开(公告)号：WO2010139948A2
公开(公告)日：2010-12-09
申请号：PCT/GB2010001083
申请日：2010-06-02
申请人： MEDICAL RES COUNCIL , UNIV NORTH CAROLINA STATE , NGUYEN DUY P , NEUMANN HEINZ , DEITERS ALEXANDER , CHIN JASON , LUSIC HRVOJE
发明人： NGUYEN DUY P , NEUMANN HEINZ , DEITERS ALEXANDER , CHIN JASON , LUSIC HRVOJE
IPC分类号： C12N15/70
CPC分类号： C12N15/67 , C07C233/49 , C07C271/22 , C12N9/93 , C12P21/02
摘要： The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.
摘要翻译：本发明涉及一种制备包含正交官能团的多肽的方法，所述正交官能团由脂肪族氨基酸或氨基酸衍生物构成，所述方法包括提供宿主细胞; 提供编码目的多肽的核酸; 提供与所述宿主细胞正交的tRNA-tRNA合成酶对; 添加包含所述正交官能团的氨基酸或氨基酸衍生物，其中所述氨基酸或氨基酸衍生物是所述正交tRNA合成酶的底物，其中所述氨基酸或氨基酸衍生物具有脂族碳骨架; 并孵育以允许所述氨基酸或氨基酸衍生物通过正交的tRNA-tRNA合成酶对引入目的多肽。本发明还涉及某些氨基酸，以及包含其的多肽。

6. 发明申请

WO2011107747A8 GENETICALLY ENCODED PHOTOCONTROL 审中-公开
标题翻译：基因编码光子
公开(公告)号：WO2011107747A8
公开(公告)日：2012-09-27
申请号：PCT/GB2011000304
申请日：2011-03-04
申请人： MEDICAL RES COUNCIL , CHIN JASON , NGUYEN DUY P , GAUTIER ARNAUD , DEITERS ALEXANDER , UNIV NORTH CAROLINA STATE
发明人： CHIN JASON , NGUYEN DUY P , GAUTIER ARNAUD , DEITERS ALEXANDER
IPC分类号： C12N9/00 , C12N9/10 , C12N15/74
CPC分类号： C12N9/1205 , C07D317/50 , C07K14/805 , C12N9/93 , C12Y207/12002 , C12Y601/01026
摘要： The invention relates to a caged lysine, wherein the caged lysine is according to Formula (I): or salts thereof. The invention further relates to polypeptides comprising a caged lysine, and to methods of making same. The invention further relates to tRNA synthetases capable of charging tRNA with caged lysine.
摘要翻译：本发明涉及笼状赖氨酸，其中笼养的赖氨酸是根据式（I）：或其盐。本发明进一步涉及包含笼养赖氨酸的多肽及其制备方法。本发明还涉及能够将tRNA与笼养的赖氨酸一起加入的tRNA合成酶。

7. 发明申请

WO2011107747A3 GENETICALLY ENCODED PHOTOCONTROL 审中-公开
标题翻译：基因编码的光控制
公开(公告)号：WO2011107747A3
公开(公告)日：2011-12-29
申请号：PCT/GB2011000304
申请日：2011-03-04
申请人： MEDICAL RES COUNCIL , CHIN JASON , NGUYEN DUY P , GAUTIER ARNAUD , DIETERS ALEXANDER , UNIV NORTH CAROLINA
发明人： CHIN JASON , NGUYEN DUY P , GAUTIER ARNAUD , DIETERS ALEXANDER
IPC分类号： C12N9/00 , C12N9/10 , C12N15/74
CPC分类号： C12N9/1205 , C07D317/50 , C07K14/805 , C12N9/93 , C12Y207/12002 , C12Y601/01026
摘要： The invention relates to a caged lysine, wherein the caged lysine is according to Formula (I): or salts thereof. The invention further relates to polypeptides comprising a caged lysine, and to methods of making same. The invention further relates to tRNA synthetases capable of charging tRNA with caged lysine.
摘要翻译：本发明涉及笼蔽赖氨酸，其中笼蔽赖氨酸根据式（I）：或其盐。本发明还涉及包含笼蔽赖氨酸的多肽，并涉及制备它的方法。本发明进一步涉及能够用笼蔽赖氨酸对tRNA进行充电的tRNA合成酶。

8. 发明申请

WO2011117583A2 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2011117583A2
公开(公告)日：2011-09-29
申请号：PCT/GB2011/000420
申请日：2011-03-23
申请人： MEDICAL RESEARCH COUNCIL , CHIN, Jason , NGUYEN, Duy, P. , VIRDEE, Satpal
发明人： CHIN, Jason , NGUYEN, Duy, P. , VIRDEE, Satpal
IPC分类号： C07K14/00 , C12N9/00
CPC分类号： C07K14/4702 , C07K1/064 , C07K1/065 , C07K1/068 , C07K1/1075 , C07K1/1077 , C07K14/435 , C12N9/50 , C12N9/93 , C12P21/02 , C12P21/06 , Y02P20/55
摘要： The invention relates to a method of modifying a specific lysine residue in a polypeptide comprising at least two lysine residues, said method comprising (a) providing a polypeptide comprising a target lysine residue protected by a first protecting group, and at least one further lysine residue; (b) treating the polypeptide to protect said further lysine residue(s), wherein the protecting group for said further lysine residues is different to the protecting group for the target lysine residue; (c) selectively deprotecting the target lysine residue; and (d) modifying the deprotected lysine residue of (c).
摘要翻译：本发明涉及一种修饰包含至少两个赖氨酸残基的多肽中的特定赖氨酸残基的方法，所述方法包括（a）提供包含被第一保护基团保护的靶赖氨酸残基的多肽和至少一种赖氨酸残基 ; （b）处理多肽以保护所述另外的赖氨酸残基，其中所述另外的赖氨酸残基的保护基与靶赖氨酸残基的保护基不同; （c）选择性脱保护目标赖氨酸残基; 和（d）修饰（c）的去保护的赖氨酸残基。

9. 发明申请

WO2010139948A3 METHOD FOR INCORPORATING ALIPHATIC AMINO ACIDS COMPRISING ALKYNE, AZIDE OR ALIPHATIC KETONE FUNCTIONAL GROUPS USING APPROPRIATE TRNA / TRNA SYNTHASE PAIRS 审中-公开
公开(公告)号：WO2010139948A3
公开(公告)日：2010-12-09
申请号：PCT/GB2010/001083
申请日：2010-06-02
申请人： MEDICAL RESEARCH COUNCIL , NORTH CAROLINA STATE UNIVERSITY , NGUYEN, Duy, P. , NEUMANN, Heinz , DEITERS, Alexander , CHIN, Jason , LUSIC, Hrvoje
发明人： NGUYEN, Duy, P. , NEUMANN, Heinz , DEITERS, Alexander , CHIN, Jason , LUSIC, Hrvoje
IPC分类号： C12N15/67
摘要： The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.

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