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1. 发明申请

WO2009104825A1 METHOD FOR INDUCING THE DEFFERENTIATION OF EMBRYONIC STEM CELLS INTO HEMANGIOBLAST 审中-公开
标题翻译：诱导胚胎干细胞分化为HEMANGIOBLAST的方法
公开(公告)号：WO2009104825A1
公开(公告)日：2009-08-27
申请号：PCT/KR2008/000952
申请日：2008-02-18
申请人： KAIST , HAN, Yong-Mahn , PARK, Sang-Wook , LEE, Eun-Young
发明人： HAN, Yong-Mahn , PARK, Sang-Wook , LEE, Eun-Young
IPC分类号： C12N5/08 , C12N5/02
CPC分类号： C12N5/069 , C12N5/0647 , C12N5/0691 , C12N5/0692 , C12N2501/115 , C12N2501/155 , C12N2501/165 , C12N2501/999 , C12N2506/02 , C12N2506/28
摘要： The present invention relates to a composition for inducing embryonic stem cell differentiation comprising a MEK/ERK (mitogen-activated protein kinase kinase/extracellular regulated kinase) signal transduction inhibitor and BMP (bone morphogenetic protein), and a method for inducing differentiation of embryonic stem cells into mesodermal cells using the same. Further, the mesodermal cells obtained by the above method are able to differentiate into various mesenchymal tissue cells. In particular, the present invention relates to a method for inducing differentiation into hemangioblast by culturing the mesodermal cells obtained by the above method in the presence of VEGF (vascular endothelial cell growth factor) and bFGF (basic fibroblast growth factor). The differentiated hemangioblasts can be further differentiated into vascular endothelial cells, vascular smooth muscle cells, and hematopoietic stem cells under various culture conditions.
摘要翻译：本发明涉及一种用于诱导胚胎干细胞分化的组合物，其包含MEK / ERK（丝裂原活化蛋白激酶激酶/细胞外调节激酶）信号转导抑制剂和BMP（骨形态发生蛋白）），以及诱导胚胎干细胞分化的方法细胞使用相同的细胞进入中胚层细胞。此外，通过上述方法获得的中胚层细胞能够分化成各种间充质组织细胞。特别地，本发明涉及通过在VEGF（血管内皮细胞生长因子）和bFGF（碱性成纤维细胞生长因子）的存在下培养通过上述方法获得的中胚层细胞，诱导分化成成血管细胞的方法。分化的成血管细胞可以在各种培养条件下进一步分化成血管内皮细胞，血管平滑肌细胞和造血干细胞。

2. 发明申请

WO2011025179A2 METHOD OF INDUCING THE DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO MESENCHYMAL STEM CELLS 审中-公开
标题翻译：诱导人胚胎干细胞分化为间质干细胞的方法
公开(公告)号：WO2011025179A2
公开(公告)日：2011-03-03
申请号：PCT/KR2010/005471
申请日：2010-08-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , HAN, Yong-Mahn , TUNG, Tran-Ngoc , PARK, Sang-Wook , JANG, Mi-jin
发明人： HAN, Yong-Mahn , TUNG, Tran-Ngoc , PARK, Sang-Wook , JANG, Mi-jin
IPC分类号： C12N5/00 , C12N7/00
CPC分类号： C12N5/0606 , C12N5/0662 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2501/415 , C12N2501/999 , C12N2506/02
摘要： Disclosed is a method for inducing human embryonic stem cells to differentiate into mesenchymal stem cells, using an optimal medium. Human embryonic stem cells are induced differentiate into mesodermal progenitors by activating TGF-β (Transforming growth factor-β), WNT and BMP (Bone morphogenic protein) signaling pathways and further into mesenchymal stem cells by treating with bFGF. The method is effective for producing mesenchymal stem cells, useful cell-based therapies, on a large scale.
摘要翻译：公开了使用最佳培养基诱导人胚胎干细胞分化为间充质干细胞的方法。通过激活TGF-β（转化生长因子-β），WNT和BMP（骨形态发生蛋白）信号传导途径并通过用bFGF处理进一步进入间充质干细胞，诱导人胚胎干细胞分化成中胚层祖细胞。该方法对于大规模生产间充质干细胞是有效的基于细胞的疗法是有效的。

3. 发明申请

WO2006083057A1 DROSOPHILA SHORT NEUROPEPTIDE F REGULATING FEEDING BEHAVIOR 审中-公开
标题翻译： DROSOPHILA短神经元F调节饲养行为
公开(公告)号：WO2006083057A1
公开(公告)日：2006-08-10
申请号：PCT/KR2005/000770
申请日：2005-03-17
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , YU, Kweon , LEE, Kyu-Sun , HAN, Yong-Mahn
发明人： YU, Kweon , LEE, Kyu-Sun , HAN, Yong-Mahn
IPC分类号： C07K14/435
CPC分类号： C07K14/43581
摘要： The present invention relates to the short neuropeptide F isolated from Drosophila melanogaster which is involved in regulating feeding behavior. More specifically, the present invention relates to the Drosophila short neuropeptide F regulating feeding behavior and the gene encoding the protein, wherein the protein has an activity similar to that of the neuropeptide Y of mammals, while being able to isolate target genes thus enabling to perform in vivo studies of the relevant genes thereby being able to serve as a model for identifying the mechanism of feeding behavior in animals.
摘要翻译：本发明涉及从果蝇黑腹果蝇分离的短神经肽F，其涉及调节进食行为。更具体地，本发明涉及果蝇短神经肽F调节进食行为和编码蛋白质的基因，其中蛋白质具有与哺乳动物的神经肽Y的活性相似的活性，同时能够分离靶基因，从而能够执行相关基因的体内研究，从而能够作为识别动物饲养行为机制的模型。

4. 发明申请

WO2006057466A1 BETA-CASEIN GENE TARGETING VECTOR USING HOMOLOGOUS RECOMBINATION 审中-公开
标题翻译： BETA-CASEIN基因靶向载体使用同源重组
公开(公告)号：WO2006057466A1
公开(公告)日：2006-06-01
申请号：PCT/KR2004/003034
申请日：2004-11-23
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , HAN, Yong-Mahn , LEE, Kyung-Kwang , CHANG, Mira , KOO, Deog-Bon
发明人： HAN, Yong-Mahn , LEE, Kyung-Kwang , CHANG, Mira , KOO, Deog-Bon
IPC分类号： C12N15/85
CPC分类号： C12N15/8771 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2267/01 , C12N15/8509 , C12N2830/008
摘要： The present invention relates to a beta-casein gene targeting vector comprising (1) a first region having a length of 5 to 12 kb which is homologous to the promoter and its flanking nucleic acid sequences of beta-casein gene; (2) a region for cloning a nucleic acid coding for desired proteins; (3) a region for coding a positive selection marker; (4) a second region having a length of 2 to 4 kb which is homologous to the nucleic acid sequences of beta-casein gene; wherein the nucleic acid segment corresponding to the first region is located upstream to the nucleic acid segment corresponding to the second region in the 5'-3' arrangement of beta-casein gene, animal cells which are gene-targeted with the said vector and embryos which are nuclear-transferred with the said animal cells. The present invention also relates to a method for obtaining a desired protein from the milk of transgenic animal which is prepared by implanting the said embryos.
摘要翻译：本发明涉及β-酪蛋白基因靶向载体，其包含（1）与启动子及其β-酪蛋白基因的侧翼核酸序列同源的长度为5至12kb的第一区域; （2）克隆编码所需蛋白质的核酸的区域; （3）用于编码正选择标记的区域; （4）与β-酪蛋白基因的核酸序列同源的长度为2〜4kb的第二区域; 其中对应于第一区域的核酸区段位于与β-酪蛋白基因的5'-3'排列中的第二区域相对应的核酸区段的上游，所述动物细胞与所述载体和胚胎进行基因靶向其与所述动物细胞进行核转移。本发明还涉及从通过植入所述胚胎制备的转基因动物的乳中获得所需蛋白质的方法。

5. 发明申请

WO2006057499A1 CATTLE BETA-CASEIN GENE TARGETING VECTOR USING HOMOLOGOUS RECOMBINATION 审中-公开
标题翻译： CATTLE BETA-CASEIN基因导向矢量使用同源重建
公开(公告)号：WO2006057499A1
公开(公告)日：2006-06-01
申请号：PCT/KR2005/003923
申请日：2005-11-18
申请人： Korea Research institute of Bioscience and Biotechnology , HAN, Yong-Mahn , LEE, Kyung-Kwang , CHANG, Mira , KOO, Deog-Bon
发明人： HAN, Yong-Mahn , LEE, Kyung-Kwang , CHANG, Mira , KOO, Deog-Bon
IPC分类号： C12N15/85 , C12N15/00 , C12N15/63 , C07H21/02 , A01H5/00
CPC分类号： C12N15/8771 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A01K2267/01 , C12N15/8509 , C12N2830/008
摘要： The present invention relates to a bovine beta-casein gene targeting vector comprising (1) a first region having a length of 5 to 12 kb which is homologous to the promoter and its flanking nucleic acid sequences of bovine beta-casein gene, and comprising exon 1, intron 1, and exon 2 of bovine beta-casein gene; (2) a region for cloning a nucleic acid coding for desired proteins; (3) a region for coding a positive selection marker; (4) a second region having a length of 2.8 to 3.5 kb which is homologous to the nucleic acid sequences of bovine beta-casein gene, and comprising exon 5, 6, 7 and 8, and intron 5, 6 and 7 of bovine beta-casein gene; wherein the nucleic acid segment corresponding to the first region is located upstream to the nucleic acid segment corresponding to the second region in the 5' -3' arrangement of beta-casein gene. The present invention also relates to method producing the transgenic cattle which is bovine beta-casein gene-targeted with a gene coding a desired protein using the said vector and obtaining a large scale of a desired protein from the milk of the said transgenic cattle.
摘要翻译：本发明涉及一种牛β-酪蛋白基因靶向载体，其包含（1）长度为5至12kb的第一区，其与牛β-酪蛋白基因的启动子及其侧翼核酸序列同源，并且包含外显子 1，牛β酪蛋白基因的内含子1和外显子2; （2）克隆编码所需蛋白质的核酸的区域; （3）用于编码正选择标记的区域; （4）具有与牛β-酪蛋白基因的核酸序列同源的2.8至3.5kb长度的第二区域，并且包含外周血5,6,7和8以及牛β内含子5,6和7 -casein基因; 其中对应于第一区域的核酸区段位于对应于β-酪蛋白基因的5'-3'排列中的第二区域的核酸区段的上游。本发明还涉及生产转基因牛的方法，该转基因牛是使用所述载体用编码所需蛋白质的基因靶向的牛β-酪蛋白基因，并从所述转基因牛的奶中获得大规模的所需蛋白质。

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