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    • 2. 发明申请
    • CATTLE BETA-CASEIN GENE TARGETING VECTOR USING HOMOLOGOUS RECOMBINATION
    • CATTLE BETA-CASEIN基因导向矢量使用同源重建
    • WO2006057499A1
    • 2006-06-01
    • PCT/KR2005/003923
    • 2005-11-18
    • Korea Research institute of Bioscience and BiotechnologyHAN, Yong-MahnLEE, Kyung-KwangCHANG, MiraKOO, Deog-Bon
    • HAN, Yong-MahnLEE, Kyung-KwangCHANG, MiraKOO, Deog-Bon
    • C12N15/85C12N15/00C12N15/63C07H21/02A01H5/00
    • C12N15/8771A01K67/0275A01K2217/05A01K2227/101A01K2267/01C12N15/8509C12N2830/008
    • The present invention relates to a bovine beta-casein gene targeting vector comprising (1) a first region having a length of 5 to 12 kb which is homologous to the promoter and its flanking nucleic acid sequences of bovine beta-casein gene, and comprising exon 1, intron 1, and exon 2 of bovine beta-casein gene; (2) a region for cloning a nucleic acid coding for desired proteins; (3) a region for coding a positive selection marker; (4) a second region having a length of 2.8 to 3.5 kb which is homologous to the nucleic acid sequences of bovine beta-casein gene, and comprising exon 5, 6, 7 and 8, and intron 5, 6 and 7 of bovine beta-casein gene; wherein the nucleic acid segment corresponding to the first region is located upstream to the nucleic acid segment corresponding to the second region in the 5' -3' arrangement of beta-casein gene. The present invention also relates to method producing the transgenic cattle which is bovine beta-casein gene-targeted with a gene coding a desired protein using the said vector and obtaining a large scale of a desired protein from the milk of the said transgenic cattle.
    • 本发明涉及一种牛β-酪蛋白基因靶向载体,其包含(1)长度为5至12kb的第一区,其与牛β-酪蛋白基因的启动子及其侧翼核酸序列同源,并且包含外显子 1,牛β酪蛋白基因的内含子1和外显子2; (2)克隆编码所需蛋白质的核酸的区域; (3)用于编码正选择标记的区域; (4)具有与牛β-酪蛋白基因的核酸序列同源的2.8至3.5kb长度的第二区域,并且包含外周血5,6,7和8以及牛β内含子5,6和7 -casein基因; 其中对应于第一区域的核酸区段位于对应于β-酪蛋白基因的5'-3'排列中的第二区域的核酸区段的上游。 本发明还涉及生产转基因牛的方法,该转基因牛是使用所述载体用编码所需蛋白质的基因靶向的牛β-酪蛋白基因,并从所述转基因牛的奶中获得大规模的所需蛋白质。