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1. 发明申请

WO2008144588A3 RAPID PROTEIN LABELING AND ANALYSIS 审中-公开
标题翻译：快速蛋白标记和分析
公开(公告)号：WO2008144588A3
公开(公告)日：2009-02-05
申请号：PCT/US2008064014
申请日：2008-05-16
申请人： INVITROGEN CORP , DILLER THOMAS , HANSON GEORGE , SUTTON BETHANY , UPDYKE TIMOTHY
发明人： DILLER THOMAS , HANSON GEORGE , SUTTON BETHANY , UPDYKE TIMOTHY
IPC分类号： C12Q1/37 , C12Q1/68 , G01N33/00 , G01N33/53
CPC分类号： G01N33/582 , B33Y80/00 , G01N33/6803 , G01N2550/00
摘要： The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.
摘要翻译：本发明提供用于在细胞裂解物或蛋白质混合物中标记，分离和分析蛋白质，特别是感兴趣的特定蛋白质的方法和组合物。蛋白质用胺反应性或硫醇反应性荧光染料或胺反应性荧光试剂标记，其与位于蛋白质上的胺基团反应变为荧光。在标记步骤之后，可以分离和分析混合物内的蛋白质。在另一个实施方案中，加入可结合标记蛋白上的标签的标签结合荧光试剂以特异性标记所关注的蛋白质。

2. 发明申请

WO2012149180A2 FLUORESCENT TRACKING DYE 审中-公开
标题翻译：荧光跟踪眼镜
公开(公告)号：WO2012149180A2
公开(公告)日：2012-11-01
申请号：PCT/US2012035242
申请日：2012-04-26
申请人： LIFE TECHNOLOGIES CORP , DILLER THOMAS , UPDYKE TIMOTHY
发明人： DILLER THOMAS , UPDYKE TIMOTHY
IPC分类号： G01N33/68
CPC分类号： G01N33/6803 , G01N2496/00 , G01N2550/00
摘要： The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments provide for a fluorescent tracking that can be used to monitor the progression of the ion front through an electrophoresis gel. Additional embodiments are directed to methods for making a fluorescent tracking dye. Yet further embodiments are directed to kits containing a fluorescent tracking dye and a dual labeled protein standard.
摘要翻译：本发明提供了用于同时测定受试蛋白的分子量以及存在于电泳泳道中的受试蛋白的相对质量（即，量）的双重标记蛋白质标准。本发明还涉及适用于制备这种双重标记的蛋白质标准品的方法以及使用这种双标记蛋白质同时测定受试蛋白质的分子量和相对量的方法。另外的实施方案提供可用于通过电泳凝胶监测离子前沿的进展的荧光跟踪。另外的实施方案涉及制备荧光跟踪染料的方法。另外的实施方案涉及含有荧光跟踪染料和双标记蛋白质标准品的试剂盒。

3. 发明申请

WO2011031497A3 QUANTITATIVE FLUORESCENT PROTEIN STANDARDS 审中-公开
标题翻译：定量荧光蛋白标准
公开(公告)号：WO2011031497A3
公开(公告)日：2011-07-14
申请号：PCT/US2010046695
申请日：2010-08-25
申请人： LIFE TECHNOLOGIES CORP , DILLER THOMAS , UPDYKE TIMOTHY
发明人： DILLER THOMAS , UPDYKE TIMOTHY
IPC分类号： G01N33/68 , G01N33/52 , G01N33/58
CPC分类号： G01N33/6803 , B33Y80/00 , C07K1/26 , Y10T436/10 , Y10T436/105831
摘要： The present invention provides dual labeled protein standards useful for the simultaneous determination of the molecular weight of a subject protein as well as the relative mass (i.e., amount) of the subject protein present in an electrophoresis lane. The invention is also directed to methods suitable for the preparation of such dual labeled protein standards and to methods of using such dual labeled proteins to simultaneously determine the molecular weight and the relative amount of a subject protein. Further embodiments are directed to the use dual labeled protein standards to make a more accurate determination of the amount of a protein present in an electrophoresis lane. Yet further embodiments are directed to kits containing the presently described dual protein standards. Dual labeled protein standards made and used in accordance with the embodiments set forth herein may be used to simultaneously determine the molecular weight and the relative amount of a subject protein in real time.
摘要翻译：本发明提供了用于同时测定主题蛋白质的分子量以及存在于电泳泳道中的主题蛋白质的相对质量（即量）的双重标记的蛋白质标准品。本发明还涉及适用于制备这种双重标记的蛋白质标准物的方法，以及使用这种双重标记的蛋白质同时测定主题蛋白质的分子量和相对量的方法。进一步的实施方案涉及使用双重标记的蛋白质标准品来更精确地确定电泳泳道中存在的蛋白质的量。另外的实施方案涉及含有目前描述的双重蛋白质标准品的试剂盒。根据本文阐述的实施方案制备和使用的双重标记的蛋白质标准物可以用于实时同时测定对象蛋白质的分子量和相对量。

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