会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 1. 发明申请
    • PROBE FOR DETECTING A PARTICULAR NUCLEIC ACID SEQUENCE
    • 用于检测特定核酸序列的探针
    • WO2008142571A2
    • 2008-11-27
    • PCT/IB2008/002111
    • 2008-05-21
    • HUMBOLDT-UNIVERSITÄT ZU BERLINSEITZ, OliverGROSSMANN, Tom, N.RÖGLIN, Lars
    • SEITZ, OliverGROSSMANN, Tom, N.RÖGLIN, Lars
    • C12Q1/68
    • C12Q1/6818C12Q1/6832C12Q2537/119C12Q2525/301
    • The invention relates to a detection molecule (triplex Molecular Beacon, tMB) for detecting a particular nucleic acid sequence, in particular for detecting a single nucleotide mismatch in a target nucleic acid. Such a detection molecule comprises a) an oligonucleotide, and b) a probe comprising a first stem forming portion with a fluorophore for generating a detection signal, a second stem forming portion with a quencher for quenching the detection signal from the fluorophore when the quencher is in proximity to the fluorophore, and a loop forming portion located between the first and the second stem forming portion, for allowing the first stem forming portion to form a stem structure together with the second stem forming portion of the probe and with the oligonucleotide. According to the invention, the oligonucleotide comprises a portion allowing it to form a triplex structure with the first and second stem forming portions of the probe.
    • 本发明涉及用于检测特定核酸序列的特异性检测分子(三重分子信标(triplex Molecular Beacon,tMB)),特别是用于检测目标核酸中的单核苷酸错配。 这样的检测分子包含:a)寡核苷酸,和b)包含具有产生检测信号的荧光团的第一茎形成部分的探针,当猝灭剂为猝灭剂时,具有猝灭剂的第二茎形成部分用于猝灭来自荧光团的检测信号 位于第一和第二茎形成部分之间的环形部分,用于允许第一茎形成部分与探针的第二茎形成部分和寡核苷酸一起形成茎结构。 根据本发明,寡核苷酸包括允许其与探针的第一和第二茎形成部分形成三重结构的部分。