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1. 发明申请

WO2006107312A8 NANOSENSORS 审中-公开
公开(公告)号：WO2006107312A8
公开(公告)日：2007-02-15
申请号：PCT/US2005020974
申请日：2005-06-15
申请人： HARVARD COLLEGE , WANG WAYNE , CHEN CHUO , LIN KENG-HUI , FANG YING , LIEBER CHARLES M
发明人： WANG WAYNE , CHEN CHUO , LIN KENG-HUI , FANG YING , LIEBER CHARLES M
IPC分类号： G01N27/414 , G01N33/543
CPC分类号： B82Y15/00 , G01N27/4146 , G01N33/54373
摘要： The present invention generally relates to nanoscale wires for use in determining analytes suspected to be present in a sample, especially in connection with determining information about a sample containing, or suspected of containing, two or more analytes. For example, the invention can involve a competitive, uncompetitive, or non-competitive binding assay including a nanoscale wire to a sample containing a species able to interact with the retain entity to produce a product, where the sample also contains or is suspected of containing a second species able to interact with the reaction entity to prevent production of the product resulting from interaction of the first species and the reaction entity. Based upon determination of production of the product, determination of the second species in the sample can be made. In one set of embodiments, nanoscale wires can be used that have been functionalized at their surface, and/or in close proximity to their surface, for example, by immobilizing a protein or an enzyme relative to the nanoscale wire. Functionalization may permit interaction of the nanoscale wire with various analytes, and such interaction may induce a determinable change in a property of the nanoscale wire. Determination of two or more analytes, o one analyte and the suspected presence of another analyte can involve, for example, binding species to a protein or an enzyme immobilized relative to the nanoscale wire. Other aspects of the invention include assays, sensors, detectors, and/or other devices that include functionalized nanoscale wires, methods of making and/or using functionalized nanoscale wires (for example, in drug screening or high throughput screening) and the like.
摘要翻译：本发明一般涉及用于确定疑似存在于样品中的分析物的纳米尺度丝，特别是关于确定含有或怀疑含有两种或多种分析物的样品的信息。例如，本发明可涉及竞争性的，无竞争性的或非竞争性的结合测定，其包括纳米尺度的丝线，其含有能够与保留实体相互作用以产生产品的样品，其中样品还含有或怀疑含有能够与反应实体相互作用以防止由第一种和反应实体的相互作用产生的产物的第二种。基于产品生产的确定，可以确定样品中的第二种。在一组实施方案中，可以使用在其表面和/或其表面附近功能化的纳米线，例如通过相对于纳米线固定蛋白质或酶。功能化可以允许纳米尺度线与各种分析物的相互作用，并且这种相互作用可以诱导纳米尺度线的性质的可确定的变化。测定两种或更多种分析物，一种分析物和怀疑存在另一种分析物可涉及例如将物质结合到相对于纳米尺度线固定的蛋白质或酶。本发明的其它方面包括测定，传感器，检测器和/或包括功能化的纳米线的其它器件，制备和/或使用官能化的纳米线的方法（例如，在药物筛选或高通量筛选中）等。

2. 发明申请

WO2008051316A2 NANOSENSORS AND RELATED TECHNOLOGIES 审中-公开
标题翻译：纳米传感器和相关技术
公开(公告)号：WO2008051316A2
公开(公告)日：2008-05-02
申请号：PCT/US2007013700
申请日：2007-06-11
申请人： HARVARD COLLEGE , LIEBER CHARLES M , FANG YING , PATOLSKY FERNANDO
发明人： LIEBER CHARLES M , FANG YING , PATOLSKY FERNANDO
IPC分类号： C12Q1/68
CPC分类号： G01N33/551 , B82Y10/00 , C12Q1/6825 , C12Q2600/156 , G01N27/4145 , G01N27/4146 , G01N33/553 , H01L51/0049 , H01L51/0093 , C12Q2563/155
摘要： The present invention generally relates to nanotechnology and sub-microelectronic circuitry, as well as associated methods and devices, for example, nanoscale wire devices and methods for use in determining nucleic acids or other analytes suspected to be present in a sample (for example, their presence and/or dynamical information), e.g., at the single molecule level. For example, a nanoscale wire device can be used in some cases to detect single base mismatches within a nucleic acid (e.g., by determining association and/or dissociation rates). In one aspect, dynamical information such as a binding constant, an association rate, and/or a dissociation rate, can be determined between a nucleic acid or other analyte, and a binding partner immobilized relative to a nanoscale wire. In some cases, the nanoscale wire includes a first portion comprising a metal-semiconductor compound, and a second portion that does not include a metal-semiconductor compound. The binding partner, in some embodiments, is immobilized relative to at least the second portion of the nanoscale wire, and the size of the second portion of the nanoscale wire may be minimized and/or controlled in some instances. Articles and devices of size greater than the nanoscale are also included in certain embodiments. Still other aspects of the invention include assays, sensors, kits, and/or other devices that include such nanoscale wires, methods of making and/or using such nanoscale wires, or the like.
摘要翻译：本发明一般涉及纳米技术和亚微电子电路以及相关的方法和装置，例如纳米级线装置和用于确定疑似存在于样品中的核酸或其他分析物的方法（例如它们的存在和/或动态信息），例如在单分子水平。例如，在一些情况下可以使用纳米级线装置来检测核酸内的单碱基错配（例如通过确定缔合和/或解离速率）。在一个方面，可以在核酸或其他分析物与相对于纳米线固定的结合配偶体之间确定动力学信息，例如结合常数，结合速率和/或解离速率。在一些情况下，纳米级线包括包含金属半导体化合物的第一部分和不包含金属半导体化合物的第二部分。在一些实施方案中，结合配偶体相对于纳米线的至少第二部分固定，并且在一些情况下纳米线的第二部分的尺寸可以被最小化和/或控制。尺寸大于纳米级的制品和器件也包括在某些实施方案中。本发明的其他方面包括分析，传感器，套件和/或包括这种纳米线的其他装置，制造和/或使用这种纳米线的方法等。

3. 发明申请

WO2011156656A2 MULTIPLEX IMMUNE EFFECTOR MOLECULE ASSAY 审中-公开
标题翻译：多重免疫效应分子测定
公开(公告)号：WO2011156656A2
公开(公告)日：2011-12-15
申请号：PCT/US2011039874
申请日：2011-06-09
申请人： UNIV SOUTH DAKOTA , US AGRICULTURE , CHRISTOPHER-HENNINGS JANE , LAWSON STEVEN , NELSON ERIC , FANG YING , LUNNEY JOAN K
发明人： CHRISTOPHER-HENNINGS JANE , LAWSON STEVEN , NELSON ERIC , FANG YING , LUNNEY JOAN K
IPC分类号： G01N33/53 , G01N33/533
CPC分类号： G01N33/6869 , G01N33/54306 , G01N33/6863 , G01N33/6866
摘要： Methods for detecting at least seven cytokines in a porcine biological sample are provided. Also provided are multiplex assay kits that allow for the detection and quantification of the cytokines in a single reaction mixture. Use of the methods and kits for diagnosis, prognosis, and monitoring of immunity is also contemplated.
摘要翻译：提供了检测猪生物样品中至少七种细胞因子的方法。还提供了允许在单个反应混合物中检测和定量细胞因子的多重测定试剂盒。还考虑使用用于诊断，预后和免疫监测的方法和试剂盒。

4. 发明申请

WO2010062395A2 IDENTIFICATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS 审中-公开
标题翻译： PORCINE生殖和呼吸道综合症病毒的鉴定
公开(公告)号：WO2010062395A2
公开(公告)日：2010-06-03
申请号：PCT/US2009006289
申请日：2009-11-25
申请人： UNIV SOUTH DAKOTA , FANG YING , BROWN ELIZABETH M , WELBON CRAIG , NELSON ERIC A
发明人： FANG YING , BROWN ELIZABETH M , WELBON CRAIG , NELSON ERIC A
IPC分类号： G01N33/569 , C07K14/135
CPC分类号： C07K14/005 , C12N2770/10022 , G01N33/56983 , G01N2333/08
摘要： An enzyme-linked immunosorbent assay (ELISA) is based on the non-structural protein 7 (nsp7) of porcine reproductive and respiratory syndrome virus (PRRSV) and provides for the simultaneous detection and differentiation of serum antibodies directed against Type 1 (European) and Type 2 (North American) PRRSV. The invention provides a serological assay for the detection and/or differentiation of serum antibodies directed against Type 1 and/or Type 2 PRRSV utilizing PRRSV nsp7 as an antigen, and provides a diagnostic method for the detection of PRRSV infection, epidemiological surveys, and outbreak investigations. The invention may be used either alone or as a follow-up assay to determine the true status of unexpected positive results that may occur using other assays, such as the IDEXX HERDCHEK PRRS ELISA.
摘要翻译：酶联免疫吸附测定（ELISA）基于猪繁殖和呼吸综合征病毒（PRRSV）的非结构蛋白7（nsp7），并且提供针对1型（欧洲）和 2型（北美）PRRSV。本发明提供了使用PRRSV nsp7作为抗原的针对1型和/或2型PRRSV的血清抗体的检测和/或分化的血清学测定，并提供了用于检测PRRSV感染，流行病学调查和爆发的诊断方法调查。本发明可以单独使用或作为随访测定来确定可能使用其它测定法（例如IDEXX HERDCHEK PRRS ELISA）发生的意想不到的阳性结果的真实状态。

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