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1. 发明申请

WO2005080552A1 METHODS FOR GENETIC DIVERSIFICATION IN GENE CONVERSION ACTIVE CELLS 审中-公开
标题翻译：基因转化活性细胞中遗传多样性的方法
公开(公告)号：WO2005080552A1
公开(公告)日：2005-09-01
申请号：PCT/EP2005/001897
申请日：2005-02-23
申请人： GSF-FORSCHUNGSZENTRUM FÜR UMWELT UND GESUNDHEIT, GMBH , BUERSTEDDE, Jean-Marie , ARAKAWA, Hiroshi
发明人： BUERSTEDDE, Jean-Marie , ARAKAWA, Hiroshi
IPC分类号： C12N5/06
CPC分类号： C07K16/00 , C12N15/1024
摘要： The invention relates to a modified lymphoid cell having gene conversion fully or partially replaced by hypermutation, wherein said cell has no deleterious mutations in genes encoding paralogues and analogues of the RAD51 protein, and wherein said cell is capable of directed and selective genetic diversification of a target nucleic acid by hypermutation or a combination of hypermutation and gene conversion. The invention also relates to a method for diversifying any transgenic target gene in said cell. Preferably, the target gene is integrated into the immunoglobulin light or heavy chain locus by targeted integration.
摘要翻译：本发明涉及具有完全或部分被超突变替代的基因转化的修饰淋巴细胞，其中所述细胞在编码RAD51蛋白的旁系同源物和类似物的基因中没有有害突变，并且其中所述细胞能够进行定向和选择性的遗传多样化靶核酸通过超突变或超突变和基因转化的组合。本发明还涉及用于使所述细胞中的任何转基因靶基因多样化的方法。优选地，靶基因通过靶向整合整合到免疫球蛋白轻链或重链基因座中。

2. 发明申请

WO2007048618A1 METHOD FOR ENHANCING SOMATIC HYPERMUTATION, GENE CONVERSION AND CLASS SWITCH RECOMBINATION 审中-公开
标题翻译：增强血液透析，基因转换和分类开关重建的方法
公开(公告)号：WO2007048618A1
公开(公告)日：2007-05-03
申请号：PCT/EP2006/010353
申请日：2006-10-26
申请人： GSF-FORSCHUNGSZENTRUM FÜR UMWELT UND GESUNDHEIT GMBH , BUERSTEDDE, Jean-Marie , SCHÖTZ, Ulrike
发明人： BUERSTEDDE, Jean-Marie , SCHÖTZ, Ulrike
IPC分类号： C12N15/00
CPC分类号： C07K14/4702 , C07K16/00 , C12N15/1024
摘要： The invention relates to a method for enhancing somatic hypermutation, gene conversion and/or immunoglobulin class switch recombination in a cell by increasing the level of an E2A encoded protein in the cell. The invention also relates to cells and cell lines with an increased level of said activities.
摘要翻译：本发明涉及通过增加细胞中E2A编码的蛋白质的水平来增强细胞中体细胞超突变，基因转化和/或免疫球蛋白类转换重组的方法。本发明还涉及具有增加的所述活性水平的细胞和细胞系。

3. 发明申请

WO2009015904A1 FLUORESCENT GFP VARIANT DISPLAYING HIGHLY INCREASED FLUORESCENCE INTENSITY WITHOUT A SPECTRAL SHIFT 审中-公开
标题翻译：荧光显微镜荧光显示荧光强度高，无光谱转换
公开(公告)号：WO2009015904A1
公开(公告)日：2009-02-05
申请号：PCT/EP2008/006372
申请日：2008-08-01
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) , BUERSTEDDE, Jean-Marie , ARAKAWA, Hiroshi
发明人： BUERSTEDDE, Jean-Marie , ARAKAWA, Hiroshi
IPC分类号： C07K14/435
CPC分类号： C07K14/43595 , C07K2319/01 , C07K2319/20 , G01N33/533 , G01N33/582 , Y10T436/143333
摘要： The present invention relates to a nucleic acid molecule encoding a polypeptide having a fluorescence emission activity with a maximum emission at 505 to 515 nm; wherein said nucleic acid molecule is selected from the group consisting of (a) a nucleic acid molecule encoding a polypeptide having, the amino acid sequence of SEQ ID NO: 2; (b) a nucleic acid molecule having the DNA sequence of SEQ ID NO: 1; (c) a nucleic acid molecule hybridizing under stringent conditions to the complementary strand of (i.) a nucleic acid molecule of (a), wherein said nucleic acid molecule of (c) encodes a polypeptide having at the position corresponding to position 146 of SEQ NO:2 a phenylalanine and at the position corresponding to position 203 of SEQ NO:2 a threonine; or (ii) a nucleic acid molecule of (b), wherein said nucleic acid molecule of (c) has at the positions corresponding to positions 438 to 440 of SEQ ID NO: 1 a nucleotide triplet selected from the group consisting of TTT and TTC; and at the positions corresponding to positions 609 to 611 of SEQ ID NO: 1 a nucleotide triplet selected from the group consisting of ACT, ACC, ACA, ACG; wherein the polypeptide encoded by the nucleic acid molecule of (c) has a fluorescence enhanced by at least the factor of 2.5 as compared to the polypeptide having the amino acid sequence of SEQ ID NO: 10; or (d) a nucleic acid molecule degenerate with respect to the nucleic acid molecule of (b). The present invention furthermore relates to a polypeptide encoded by the nucleic acid molecule of the invention, a vector and a host cell comprising the nucleic acid molecule of the invention, a method of producing said polypeptide, a fusion protein comprising the polypeptide of the invention and methods of detecting the presence and/or localization of a protein of interest as well as methods of detecting the activity of a promoter.
摘要翻译：本发明涉及编码在505至515nm具有最大发射的荧光发射活性的多肽的核酸分子; 其中所述核酸分子选自（a）编码具有SEQ ID NO：2的氨基酸序列的多肽的核酸分子; （b）具有SEQ ID NO：1的DNA序列的核酸分子; （c）在严格条件下与（a）的核酸分子的互补链杂交的核酸分子，其中（c）的核酸分子编码在对应于位置146的位置的位置处的多肽 SEQ ID NO：2为苯丙氨酸，并且在对应于SEQ ID NO：2的203位的位置为苏氨酸; 或（ii）（b）的核酸分子，其中所述（c）的核酸分子在与SEQ ID NO：1的438〜440位相对应的位置处选自TTT和TTC的核苷酸三联体 ; 在与SEQ ID NO：1的位置609〜611相对应的位置，选自ACT，ACC，ACA，ACG的核苷酸三联体; 其中由（c）的核酸分子编码的多肽与具有SEQ ID NO：10的氨基酸序列的多肽相比，其荧光增强至少2.5倍; 或（d）相对于（b）的核酸分子简并的核酸分子。本发明还涉及由本发明的核酸分子编码的多肽，载体和包含本发明的核酸分子的宿主细胞，产生所述多肽的方法，包含本发明的多肽的融合蛋白和检测感兴趣的蛋白质的存在和/或定位的方法以及检测启动子的活性的方法。

4. 发明申请

WO2009124777A1 CIS-ACTING DIVERSIFICATION ACTIVATOR AND METHOD FOR SELECTIVE DIVERSIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：多样化ACTIVATOR ACTING <我
公开(公告)号：WO2009124777A1
公开(公告)日：2009-10-15
申请号：PCT/EP2009/002680
申请日：2009-04-09
申请人： HELMHOLTZ ZENTRUM MÜNCHEN DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) , BUERSTEDDE, Jean-Marie , CALDWELL, Randolph, B.
发明人： BUERSTEDDE, Jean-Marie , CALDWELL, Randolph, B.
IPC分类号： C12N15/00 , C12N15/01 , C12N5/06
CPC分类号： C12N15/1024
摘要： The invention relates to identification of a cis -acting diversification activator ( DIVAC ) that is necessary and sufficient for the activation of diversification in transcription units linked thereto. The invention provides a method for diversification of a target nucleic acid comprising introducing a genetic construct comprising the diversification activator into a recipient cell, wherein the diversification activator is linked to the target nucleic acid.
摘要翻译：公开了一种多样化活化剂的顺式作用（迪瓦茨）是必要的和足够的多样化在转录单位与其连接的激活的识别。本发明提供一种靶核酸，其包含导入的基因构建体，其包括多样化活化剂到受体细胞的多样化的方法，所述多样化活化剂连接到靶核酸。

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