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1. 发明申请

WO2011060557A1 METHOD FOR SELECTION OF APTAMERS 审中-公开
标题翻译：选择APTAMERS的方法
公开(公告)号：WO2011060557A1
公开(公告)日：2011-05-26
申请号：PCT/CA2010/001887
申请日：2010-11-22
申请人： GOVERNORS OF THE UNIVERSITY OF ALBERTA , LI, Xing-Fang , ZHANG, Jing , LIU, Yanming , LE, X. Chris
发明人： LI, Xing-Fang , ZHANG, Jing , LIU, Yanming , LE, X. Chris
IPC分类号： C12Q1/68 , C40B30/04 , C12N15/115
CPC分类号： C12N15/115 , C12N2310/16 , C12N2330/31 , C12Q1/6811 , C12Q2600/136 , G01N33/5308 , C12Q2565/519 , C12Q2525/205
摘要： A method of selecting aptamers which specifically bind to target molecules is provided in which the target molecules are separated by gel electrophoresis and transferred onto a membrane. The membrane is incubated with a randomized mixture of aptamers, particularly single stranded DNA, under conditions to form aptamer-target molecule complexes on the membrane. The membrane is washed or contacted with DNAse I to remove unbound aptamers and weakly bound aptamers. The bound aptamers are eluted from the aptamer-target molecule complexes, amplified and sequenced.
摘要翻译：提供选择特异性结合靶分子的适体的方法，其中通过凝胶电泳分离靶分子并转移到膜上。在膜上形成适体 - 靶分子复合物的条件下，将膜与适配体，特别是单链DNA的随机混合物一起温育。将膜洗涤或与DNA酶I接触以除去未结合的适体和弱结合的适体。结合的适体从适体 - 靶分子复合物中洗脱，扩增和测序。

2. 发明申请

WO2011109901A1 DETECTION AND QUANTIFICATION OF VIABLE BACTERIA IN A SAMPLE USING RPOS MRNA 审中-公开
标题翻译：使用RPOS MRNA在样品中检测和定量可见细菌
公开(公告)号：WO2011109901A1
公开(公告)日：2011-09-15
申请号：PCT/CA2011/000261
申请日：2011-03-10
申请人： THE GOVERNORS OF THE UNIVERSITY OF ALBERTA , LI, Xing-Fang , LIU, Yanming
发明人： LI, Xing-Fang , LIU, Yanming
IPC分类号： C12Q1/68 , C12Q1/06
CPC分类号： C12Q1/689 , C12Q1/6851 , C12Q2600/158
摘要： A method and kit for detecting and quantifying viable bacteria in a sample is provided. The bacteria can be Escherichia coli O157:H7 and can be detected and quantified using a selective marker within the rpoS gene. Specifically designed primers and probe combinations can differentiate E. coli O157:H7 from closely related bacteria and other common bacteria. A real-time reverse transcription polymerase chain reaction on mRNA extracted from the cells can determine a threshold cycle value of the polymerase chain reaction which can be used to detect and quantify viable but nonculturable bacteria in the sample. The method and kit can detect and quantify viable bacteria even if the bacteria is nonculturable.
摘要翻译：提供了用于检测和定量样品中的活细菌的方法和试剂盒。细菌可以是大肠杆菌O157：H7，可以使用rpoS基因内的选择性标记进行检测和定量。具体设计的引物和探针组合可以区分大肠杆菌O157：H7与密切相关的细菌和其他常见细菌。从细胞提取的mRNA上的实时逆转录聚合酶链反应可以确定聚合酶链反应的阈值循环值，其可以用于检测和定量样品中可行但不可培养的细菌。即使细菌是不可培养的，该方法和试剂盒也可以检测和定量活细菌。

3. 发明申请

WO2011022820A1 BINDING-INDUCED HAIRPIN DETECTION SYSTEM 审中-公开
标题翻译：绑定诱发的毛发检测系统
公开(公告)号：WO2011022820A1
公开(公告)日：2011-03-03
申请号：PCT/CA2010/001303
申请日：2010-08-30
申请人： THE GOVERNORS OF THE UNIVERSITY OF ALBERTA , LE, Xiaochun , ZHANG, Hongquan , LI, Xing-Fang
发明人： LE, Xiaochun , ZHANG, Hongquan , LI, Xing-Fang
IPC分类号： C12Q1/68 , C40B30/04 , G01N33/53
CPC分类号： G01N33/53 , C12Q1/6816 , G01N33/542 , G01N2458/10 , C12Q2525/301
摘要： This application relates to a binding-induced hairpin detection system comprising: a. a first probe comprising a targeting molecule and an oligonucleotide that has a free end and an end attached to the targeting molecule; and b. a second probe comprising a targeting molecule and an oligonucleotide that has an end attached to the targeting molecule and a free end comprising a nucleotide sequence that is complementary to a nucleotide sequence at or near the free end of the oligonucleotide of the first probe; wherein upon binding of the targeting molecule to a target molecule, the free end of the oligonucleotide of the second probe hybridizes at or near the free end of the oligonucleotide of the first probe forming a hairpin stem, the non-hybridized portions of the first and second probes together with the target molecule bound thereto forming a hairpin loop, thereby providing a binding-induced hairpin.
摘要翻译：本申请涉及绑定诱导的发夹检测系统，包括：a。第一探针，其包含靶向分子和寡核苷酸，所述寡核苷酸具有连接于所述靶向分子的游离末端和末端; 和b。第二探针，其包含靶向分子和具有连接于所述靶向分子的末端的寡核苷酸，以及包含与所述第一探针的寡核苷酸的游离末端或其附近的核苷酸序列互补的核苷酸序列的自由端; 其中当靶向分子与靶分子结合时，第二探针的寡核苷酸的游离末端在形成发夹茎的第一探针的寡核苷酸的自由端或其附近杂交，第一和第二探针的非杂交部分第二探针与结合的靶分子一起形成发夹环，从而提供结合诱导的发夹。

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