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1. 发明申请

WO2010013359A1 EFFICIENT METHOD FOR ESTABLISHING INDUCED PLURIPOTENT STEM CELLS 审中-公开
标题翻译：建立诱导型脑干细胞的有效方法
公开(公告)号：WO2010013359A1
公开(公告)日：2010-02-04
申请号：PCT/JP2008/068320
申请日：2008-10-02
申请人： GIFU UNIVERSITY , Kyoto University , TEZUKA, Kenichi , SHIBATA, Toshiyuki , KUNISADA, Takahiro , TAMAOKI, Naritaka , TAKEDA, Tomoko , YAMANAKA, Shinya , TAKAHASHI, Kazutoshi
发明人： TEZUKA, Kenichi , SHIBATA, Toshiyuki , KUNISADA, Takahiro , TAMAOKI, Naritaka , TAKEDA, Tomoko , YAMANAKA, Shinya , TAKAHASHI, Kazutoshi
IPC分类号： C12N5/00
CPC分类号： C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2506/1361 , C12N2510/00
摘要： The present invention provides a method of producing induced pluripotent stem (iPS) cells, comprising bringing a nuclear reprogramming substance into contact with dental pulp stem cells. By using dental pulp stem cells as a source of somatic cells, the efficiency of establishment of human iPS cells by transfer of 3 or 4 factors can be improved dramatically. Additionally, dental pulp stem cells are easily available because they can be isolated and prepared from extracted wisdom teeth and teeth extracted because of periodontal disease and the like, so that they can be used widely as a source of somatic cells for iPS cell banks.
摘要翻译：本发明提供了一种产生诱导多能干细胞（iPS）细胞的方法，包括使核重编程物质与牙髓干细胞接触。通过使用牙髓干细胞作为体细胞来源，可以显着改善通过转移3或4个因子来建立人iPS细胞的效率。此外，牙髓干细胞易于获得，因为它们可以由提取的智齿和由于牙周病等提取的牙齿分离和制备，使得它们可以广泛用作iPS细胞库的体细胞来源。

2. 发明申请

WO2012060473A1 METHOD OF EFFICIENTLY ESTABLISHING INDUCED PLURIPOTENT STEM CELLS 审中-公开
标题翻译：有效建立诱导型脑干细胞的方法
公开(公告)号：WO2012060473A1
公开(公告)日：2012-05-10
申请号：PCT/JP2011/076017
申请日：2011-11-04
申请人： Kyoto University , YAMANAKA, Shinya , TAKAHASHI, Kazutoshi , TANABE, Koji , HYENJONG, Hong
发明人： YAMANAKA, Shinya , TAKAHASHI, Kazutoshi , TANABE, Koji , HYENJONG, Hong
IPC分类号： C12N15/09 , C12N5/10
CPC分类号： C12N15/01 , C12N5/0696 , C12N9/12 , C12N9/18 , C12N15/86 , C12N2501/405 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/999
摘要： Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate.
摘要翻译：提供了一种提高iPS细胞建立效率的方法，其包括将核苷酸D家族中的一种或多种因子和编码该细胞的核酸与体细胞接触的一个或多个因子体细胞重编程。还提供了一种生产iPS细胞的方法，包括使因子和核重编程物质与体细胞接触的步骤，iPS细胞包含编码属于细胞周期蛋白D家族的蛋白质的核酸可以通过生产iPS细胞的方法获得，以及通过强迫iPS细胞分化的体细胞产生的方法。

3. 发明申请

WO2010137746A1 METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS AND METHOD FOR CULTURING THE SAME 审中-公开
标题翻译：用于产生诱导性的细胞干细胞的方法和用于培养它们的方法
公开(公告)号：WO2010137746A1
公开(公告)日：2010-12-02
申请号：PCT/JP2010/059493
申请日：2010-05-28
申请人： KYOTO UNIVERSITY , YAMANAKA, Shinya , TAKAHASHI, Kazutoshi
发明人： YAMANAKA, Shinya , TAKAHASHI, Kazutoshi
IPC分类号： C12N5/10
CPC分类号： C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2502/00 , C12N2502/13 , C12N2502/1323 , C12N2510/00
摘要： Induced pluripotent stem cells are produced from human somatic cells by co-culturing human somatic cells having a reprogrammed nucleus with human cells as feeder cells. Induced pluripotent stem cells are produced from somatic cells by co-culturing somatic cells having a reprogrammed nucleus with autologous cells as feeder cells. Induced pluripotent stem cells are cultured with culture supernatant of somatic cells.
摘要翻译：通过将具有重编程核的人体细胞与人细胞作为饲养细胞共培养，从人体细胞产生诱导的多能干细胞。通过将具有重编程核的体细胞与自体细胞共培养为饲养细胞，从体细胞产生诱导的多能干细胞。诱导的多能干细胞与体细胞的培养上清液一起培养。

4. 发明申请

WO2009157593A1 METHOD OF EFFICIENTLY ESTABLISHING INDUCED PLURIPOTENT STEM CELLS 审中-公开
标题翻译：有效建立诱导型脑干细胞的方法
公开(公告)号：WO2009157593A1
公开(公告)日：2009-12-30
申请号：PCT/JP2009/062173
申请日：2009-06-26
申请人： KYOTO UNIVERSITY , YAMANAKA, Shinya , TAKAHASHI, Kazutoshi , OKITA, Keisuke
发明人： YAMANAKA, Shinya , TAKAHASHI, Kazutoshi , OKITA, Keisuke
IPC分类号： C12N5/06
CPC分类号： C12N5/0696 , C12N15/85 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2506/11 , C12N2510/00
摘要： The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells, comprising inhibiting the p53 function in the step of somatic cell nuclear reprogramming. The inhibition of p53 function is achieved by bringing a substance selected from the group consisting of (1) chemical inhibitors of p53, (2) dominant negative mutants of p53 and nucleic acids that encode the same, (3) siRNAs and shRNAs against p53 and DNAs that encode the same, and (4) p53 pathway inhibitors, into contact with a somatic cell, and the like. The present invention also provides an agent for improving the efficiency of establishment of iPS cells, the agent comprising an inhibitor of p53 function, particularly (1) chemical inhibitors of p53, (2) dominant negative mutants of p53 and nucleic acids that encode the same, (3) siRNAs and shRNAs against p53 and DNAs that encode the same, and (4) p53 pathway inhibitors. The present invention further provides a method of producing an iPS cell, comprising bringing a nuclear reprogramming substance and an inhibitor of p53 function into contact with a somatic cell.
摘要翻译：本发明提供了提高诱导多能干细胞（iPS）细胞的建立效率的方法，包括抑制体细胞核重编程步骤中的p53功能。通过使选自（1）p53的化学抑制剂，（2）p53的显性失活突变体和编码其的核酸，（3）siRNA和shRNA的物质与p53和编码相同的DNA，（4）p53途径抑制剂，与体细胞接触等。本发明还提供了用于提高iPS细胞的建立效率的试剂，该试剂包含p53功能的抑制剂，特别是（1）p53的化学抑制剂，（2）p53的显性失活突变体和编码其的核酸，（3）针对p53的siRNA和shRNA，编码相同的DNA，以及（4）p53途径抑制剂。本发明还提供了一种制备iPS细胞的方法，包括使核重编程物质和p53功能抑制剂与体细胞接触。

5. 发明申请

WO2013014929A1 METHOD FOR SCREENING INDUCED PLURIPOTENT STEM CELLS 审中-公开
标题翻译：筛选诱导型脑干细胞的方法
公开(公告)号：WO2013014929A1
公开(公告)日：2013-01-31
申请号：PCT/JP2012/004747
申请日：2012-07-25
申请人： KYOTO UNIVERSITY , YAMANAKA, Shinya , TAKAHASHI, Kazutoshi , KOYANAGI, Michiyo , OHNUKI, Mari
发明人： YAMANAKA, Shinya , TAKAHASHI, Kazutoshi , KOYANAGI, Michiyo , OHNUKI, Mari
IPC分类号： C12N15/09 , C12N5/10 , C12Q1/02 , C12Q1/68 , G01N33/50 , G01N33/53
CPC分类号： C12Q1/6888 , C12Q1/6881 , C12Q2600/158 , G01N33/5023 , G01N33/5073
摘要： The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.
摘要翻译：本发明提供了使用在鉴定为表现出耐分化性的iPS细胞系中特异表达的鉴定为lincRNA或mRNA的标记物以及这些标记物来筛选显示分化抗性的iPS细胞的方法。

6. 发明申请

WO2011158960A1 METHOD FOR SELECTING HUMAN INDUCED PLURIPOTENT STEM CELLS 审中-公开
标题翻译：选择人类诱导的大脑干细胞的方法
公开(公告)号：WO2011158960A1
公开(公告)日：2011-12-22
申请号：PCT/JP2011/064195
申请日：2011-06-15
申请人： KYOTO UNIVERSITY , YAMANAKA, Shinya , TAKAHASHI, Kazutoshi , OHNUKI, Mari
发明人： YAMANAKA, Shinya , TAKAHASHI, Kazutoshi , OHNUKI, Mari
IPC分类号： C12N5/10
CPC分类号： C12N5/0081 , C12N5/0696 , C12N2501/15 , C12N2501/155
摘要： The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of:(1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells.
摘要翻译：本发明提供了可以安全地用于移植的人诱导多能干细胞（iPS）细胞的选择方法。也就是说，本发明提供了选择具有降低的分化抗性的人iPS细胞的方法，包括以下步骤：（1）诱导人iPS细胞的分化; （2）在步骤（1）之后检测剩余的未分化细胞; 和（3）选择在步骤（2）中检测到的剩余未分化细胞的速率等于或不超过对照细胞的人iPS细胞。

7. 发明申请

WO2011087154A1 METHOD FOR SCREENING INDUCED PLURIPOTENT STEM CELLS 审中-公开
标题翻译：筛选诱导型脑干细胞的方法
公开(公告)号：WO2011087154A1
公开(公告)日：2011-07-21
申请号：PCT/JP2011/051144
申请日：2011-01-17
申请人： KYOTO UNIVERSITY , YAMANAKA, Shinya , KOYANAGI, Michiyo
发明人： YAMANAKA, Shinya , KOYANAGI, Michiyo
IPC分类号： C12N5/073
CPC分类号： C12Q1/6881 , C12Q2600/158 , C12Q2600/178
摘要： The present invention relates to miRNA or genes expressed in induced pluripotent stem cells, and a method for screening for induced pluripotent stem cells having functions equivalent to those of embryonic stem cells by confirming methylation of specific gene regions of induced pluripotent stem cells.
摘要翻译：本发明涉及在诱导多能干细胞中表达的miRNA或基因，以及通过确认诱导的多能干细胞的特定基因区域的甲基化来筛选具有与胚胎干细胞相同功能的诱导多能干细胞的方法。

8. 发明申请

WO2010137348A1 METHOD FOR SELECTING CLONE OF INDUCED PLURIPOTENT STEM CELLS 审中-公开
标题翻译：选择诱导型干细胞克隆的方法
公开(公告)号：WO2010137348A1
公开(公告)日：2010-12-02
申请号：PCT/JP2010/003620
申请日：2010-05-28
申请人： KEIO UNIVERSITY , KYOTO UNIVERSITY , OKANO, Hideyuki , OKADA, Yohei , YAMANAKA, Shinya , MIURA, Kyoko
发明人： OKANO, Hideyuki , OKADA, Yohei , YAMANAKA, Shinya , MIURA, Kyoko
IPC分类号： C12N5/0735
CPC分类号： C12Q1/6881 , C12N5/0619 , C12N5/0696 , C12N2501/115 , C12N2501/385 , C12N2501/606 , C12N2506/45 , C12N2510/00 , C12Q2600/158
摘要： To efficiently identify and select a clone from clones of induced pluripotent stem cells (iPS cell) having low tumor formation rate in vivo when allowed to differentiate and transplanted in a living body, iPS cells of the clones are induced to differentiate, undifferentiated cells among the cells after the induction of differentiation are detected, and a clone having the content of the undifferentiated cell below a control is selected.
摘要翻译：为了有效地鉴定并选择在允许分化并移植到活体中的体内具有低肿瘤形成速率的诱导多能干细胞（iPS细胞）克隆的克隆中，诱导克隆的iPS细胞分化未分化细胞检测诱导分化后的细胞，并选择具有低于对照的未分化细胞含量的克隆。

9. 发明申请

WO2010050626A1 METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS 审中-公开
标题翻译：用于产生诱导的大脑干细胞的方法
公开(公告)号：WO2010050626A1
公开(公告)日：2010-05-06
申请号：PCT/JP2009/069015
申请日：2009-10-30
申请人： KYOTO UNIVERSITY , YAMANAKA, Shinya , TANABE, Koji
发明人： YAMANAKA, Shinya , TANABE, Koji
IPC分类号： C12N15/09 , C12N5/10
CPC分类号： C12N5/0696 , C12N2501/603 , C12N2501/605 , C12N2501/608 , C12N2501/727 , C12N2510/00
摘要： The present invention relates to a method for producing mammalian induced pluripotent stem cells, comprising introducing mammal-derived reprogramming factors comprising Oct3/4 and Nanog, or nucleic acids encoding Oct3/4 and Nanog, into mammal-derived somatic cells and thereby inducing induced pluripotent stem cells from the somatic cells, wherein the reprogramming factors comprise neither Sox2 nor nucleic acid encoding Sox2.
摘要翻译：本发明涉及一种用于产生哺乳动物诱导的多能干细胞的方法，其包括将包含Oct3 / 4和Nanog的哺乳动物衍生的重编程因子或编码Oct3 / 4和Nanog的核酸引入哺乳动物衍生的体细胞，从而诱导诱导的多能干细胞来自体细胞的干细胞，其中重编程因子既不包括Sox2也不包含编码Sox2的核酸。

10. 发明申请

WO2012067265A1 METHOD FOR SCREENING DRUGS FOR SUPPRESSING INFLAMMASOME ACTIVITY 审中-公开
标题翻译：用于筛选用于抑制炎症活性的药物的方法
公开(公告)号：WO2012067265A1
公开(公告)日：2012-05-24
申请号：PCT/JP2011/077265
申请日：2011-11-17
申请人： KYOTO UNIVERSITY , NAKAHATA, Tatsutoshi , SAITO, Megumu , TANAKA, Takayuki , YAMANAKA, Shinya
发明人： NAKAHATA, Tatsutoshi , SAITO, Megumu , TANAKA, Takayuki , YAMANAKA, Shinya
IPC分类号： G01N33/50 , G01N33/15
CPC分类号： G01N33/5055 , C12N5/0696 , G01N33/6869 , G01N33/6872 , G01N2333/545 , G01N2500/10
摘要： The present invention provides a method for screening drugs for suppressing inflammasome activity, using macrophages derived from induced pluripotent stem cells (iPS cells) having mutant NLRP3 gene.
摘要翻译：本发明提供了使用衍生自具有突变体NLRP3基因的诱导多能干细胞（iPS细胞）的巨噬细胞来筛选用于抑制炎症性活性的药物的方法。

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